摘要
目的建立超高效液相色谱法(UPLC)快速测定人血浆中替考拉宁的浓度。方法分析色谱柱为Acquity UPLC BEHC18(2.1 mm×50 mm,1.7μm)。流动相A为含20 mmol·L~(-1)乙酸铵的超纯水,流动相B为乙腈。柱温:35℃。流速为0.5mL·min~(-1),梯度洗脱。检测波长为215 nm。用哌拉西林钠为内标。采用SPSS对内标法和外标法的计算结果进行配对t检验,以考察两种计算结果是否存在差异。结果人血浆替考拉宁线性范围为3.125~100.00μg·mL~(-1)(r=0.999 9)。内标法与外标法计算结果无显著性差异,可相互转换。结论该方法灵敏、回收率高、重现性好,适用于人血浆中替考拉宁浓度监测,指导临床合理用药。
OBJECTIVE To establish a rapid ultra-high performance liquid chromatography method for determining teicoplanin concentration in human plasma. METHODS The samples were chromatographed on Waters Acquity UPLC BEH-C18 column using gradient elution method( mobile phase A: 20 mmol·L~(-1) ammonium acetate; mobile phase B: acetonitrile). The absorption wavelength was set at 215 nm. The column temperature were maintained at 35 ℃. The teicoplanin concentration was calculated by internal standard method and external standard method,respectively. The results of the two methods were compared by paired t-test using SPSS statisticals software. RESULTS The liner range of the calibration curve for teicoplanin was 3. 15-100 μg·mL~(-1)( r = 0. 999 9). The concentrations calculated by the two methods had good correlation without significant difference. CONCLUSION This method is proved to be rapid,sensitive and suitable for the therapeutic drug monitoring and pharmacokinetic investigation of teicoplanin in human plasma.
引文
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