雨生红球藻破壁孢子粉对小鼠酒精性损伤肾脏的保护作用研究
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摘要
为了研究雨生红球藻破壁孢子粉对酒精性损伤小鼠肾脏的保护作用,将小鼠随机分为正常对照组、模型组和雨生红球藻粉低、中、高剂量组(100 mg/kg、200 mg/kg、400 mg/kg)。其中,剂量组动物先灌胃受试物7 d,第8天开始,给予剂量组和模型组50%酒精连续灌胃7 d,建立酒精性损伤模型。最后一次灌胃16 h后处死小鼠,取血清和相关脏器,测定小鼠血清尿素氮(blood urea nitrogen, BUN)和肌酐(creatinine, CREA)浓度,同时测定肾脏中超氧化物歧化酶(superoxide dismutase, SOD)活性以及谷胱甘肽(glutathione, GSH)、丙二醛(malondialdehyde, MDA)、肿瘤坏死因子-α(tumor necrosis factor-α, TNF-α)和白细胞介素-1β(interleukin-1β, IL-β)含量。结果显示:与模型组相比,雨生红球藻破壁孢子粉各剂量组肾脏指数降低, BUN和CREA含量显著降低(P<0.01),SOD活性和GSH含量显著升高(P<0.01), MDA显著降低(P<0.01),炎性因子TNF-α和IL-β也显著降低(P<0.05)。上述实验结果表明雨生红球藻破壁孢子粉对小鼠酒精性肾脏损伤具有明显的保护作用。
To explore the kidney protective effect of Haematococcus pluvialis on alcoholic injury, mice were randomly divided into the control group, model group and groups treated with different doses(100 mg/kg,200 mg/kg and 400 mg/kg) of the H. pluvialis powder. The alcoholic liver injury model was induced by intragastric administration with 50% ethanol for 7 days. About 16 h after the last administration, the animals were killed, and the sera and kidneys were obtained for determining the related markers, including the blood urea nitrogen(BUN) and creatinine(CREA), and superoxide dismutase(SOD), glutathione(GSH), malondialdehyde(MDA), tumor necrosis factor-α(TNF-α) and interleukin-1β(IL-1β) in kidney. Compared with the model group, mice in treatment groups had a reduced kidney index, significantly decreased levels of BUN and CREA(P<0.01), significantly increased SOD activity and contents of GSH(P<0.01), and significantly decreased contents of MDA(P<0.01), and also levels of inflammatory factors(TNF-α and IL-1β)(P<0.05). These results suggested that H. pluvialis had a significant kidney protective effect on alcohol-induced injury in mice.
To explore the kidney protective effect of Haematococcus pluvialis on alcoholic injury, mice were randomly divided into the control group, model group and groups treated with different doses(100 mg/kg,200 mg/kg and 400 mg/kg) of the H. pluvialis powder. The alcoholic liver injury model was induced by intragastric administration with 50% ethanol for 7 days. About 16 h after the last administration, the animals were killed, and the sera and kidneys were obtained for determining the related markers, including the blood urea nitrogen(BUN) and creatinine(CREA), and superoxide dismutase(SOD), glutathione(GSH), malondialdehyde(MDA), tumor necrosis factor-α(TNF-α) and interleukin-1β(IL-1β) in kidney. Compared with the model group, mice in treatment groups had a reduced kidney index, significantly decreased levels of BUN and CREA(P<0.01), significantly increased SOD activity and contents of GSH(P<0.01), and significantly decreased contents of MDA(P<0.01), and also levels of inflammatory factors(TNF-α and IL-1β)(P<0.05). These results suggested that H. pluvialis had a significant kidney protective effect on alcohol-induced injury in mice.
引文
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[12]李勇超,贺青华,刘瑞雪,等.雨生红球藻源虾青素对糖尿病小鼠的降糖作用及其机制[J].食品工业科技(LI Yong-chao,HE Qing-hua,LIU Rui-xue,et al.Effect of Haematococcus pluvialis astaxanthin on diabetes mice for decreasing blood glucose and its mechanisms[J].Science and Technology of Food Industry),2016,37(24):355-359.
[13]郭艳,车茜,刘科亮.雨生红球藻软胶囊对小鼠的增强免疫力功能实验研究[J].食品与发酵科技(GUO Yan,CHE Qian,LIU Ke-liang.Immunological function of Haematococcus pluvialis soft capsule on mice[J].Food and Fermentation Technology),2016,52(3):33-36
[14]魏芬芬,王文娟,贺青华,等.雨生红球藻破壁孢子粉对酒精性肝损伤的保护作用研究[J].食品工业科技(WEI Fen-fen,WANG Wen-juan,HE Qing-hua,et al.Study on the protective effect of Haematococcus pluvialis on alcoholic liver injury[J].Science and Technology of Food Industry),2019,40(5):270-274.
[15]GARAYCOECHEA J I,CROSSAN G P,LANGEVIN F,et al.Alcohol and endogenous aldehydes damage chromosomes and mutate stem cells[J].Nature,2018,553(7687):171-177.
[16]ROSWALL N,WEIDERPASS E.Alcohol as a risk factor for cancer:existing evidence in a global perspective[J].Journal of Preventive Medicine and Public Health,2015,48(1):1-9.
[17]李亚琳,权美平,陆苗.四氯化碳联合二乙基亚硝胺对小鼠肾脏毒性作用的研究[J].生命科学研究(LI Ya-lin,QUAN Meiping,LU Miao.Study on the toxicity of carbon tetrachloride combining with diethylnitrosamine to mouse kidney tissue[J].Life Science Research,2017,21(1):6-9.
[18]刘莹莹,武俊紫,李伟,等.金钗石斛多糖对2型糖尿病大鼠心肌组织凋亡相关蛋白的影响[J].中西医结合心脑血管病杂志(LIU Ying-ying,WU Jun-zi,LI Wei,et al.Effects of Dendrobium nobile polysaccharides on apoptosis related proteins of alleviates myocardium in type 2 diabetic rats[J].Chinese Journal of Integrative Medicine on Cardio-Cerebrovascular Disease),2018,16(18):2621-2626.
[19]齐玉增,苟欣,周青松,等.骨髓间充质干细胞移植在小鼠急性肾损伤肾内的存活与分化情况研究[J].生命科学研究(QIYu-zeng,GOU Xin,ZHOU Qing-song,et al.Survival and differentiation of transplanted bone marrow mesenchymal stem cells in kidneys of acute renal injury mice[J].Life Science Research),2011,15(6):471-475.
[20]王楠,蔡夏夏,鲍雷,等.膳食5′-核苷酸对大鼠酒精性肾损伤保护作用的研究[J].科技导报(WANG Nan,CAI Xia-xia,BAO Lei,et al.Protective effect of dietary 5′-nucleotides on alcohol-induced kidney injury of mice[J].Science and Technology Review),2017,35(10):64-70.
[21]CHOI S H,LEE A Y,PARK C H,et al.Protective effect of Carthamus tinctorius L.seed on oxidative stress and cognitive impairment induced by chronic alcohol consumption in mice[J].Food Science and Biotechnology,2018,27(5):1475-1484.
[22]WU B Y,ZHU M,RUAN T,et al.Oxidative stress,apoptosis and abnormal expression of apoptotic protein and gene and cell cycle arrest in the cecal tonsil of broilers induces by dietary methionine deficiency[J].Research in Veterinary Science,2018,121:65-75.
[23]WU F,HUANG W,LIU Q,et al.Responses of antioxidant defense and immune gene expression in early life stages of large yellow croaker(Pseudosciaena crocea)under methyl mercury exposure[J].Frontiers in Physiology,2018,DOI:10.3389/fphys.2018.01436.
[24]王潮岗,韩燊,陈甄倩,等.雨生红球藻抗氧化系统对活性氧的清除机制[J].水生生物学报(WANG Chao-gang,HAN Shen,CHEN Zhen-qian,et al.The scavenging of reactive oxygen species with antioxidant systems in Haematococcus pluvialis[J].Acta Hydrobiologica Sinica),2012,36(4):804-808.
[25]YIN Y,LIU X,LIU J,et al.Beta-sitosterol and its derivatives repress lipopolysaccharide/D-galactosamine-induced acute hepatic injury by inhibiting the oxidation and inflammation in mice[J].Bioorganic&Medicinal Chemistry Letters,2018,28(9):1525-1533.
[2]TANAKA K,TAKAHASHI H,HYOGO H,et al.Epidemiological survey of hemoglobin A1c and liver fibrosis in a general population with non-alcoholic fatty liver disease[J].Hepatology Research,2018,DOI:10.1111/hepr.13282.
[3]YUAN Q,HONG S,HAN S,et al.Preconditioning with physiological levels of ethanol protect kidney against ischemia/reperfusion injury by modulating oxidative stress[J].PLoS One,2011,6(10):e25811.
[4]LEAL S,RICARDO JORGE D,JOANA B,et al.Heavy alcohol consumption effects on blood pressure and on kidney structure persist after long-term withdrawal[J].Kidney and Blood Pressure Research,2017,42(4):664-675.
[5]MOAN I S,STORVOLL E E,LUND I O.Worries about others’substance use-Differences between alcohol,cigarettes and illegal drugs?[J].International Journal of Drug Policy,2017,48:108-114.
[6]鲁政,张静,高兆兰,等.牛蒡菊糖和菊芋菊糖对酒精诱导大鼠慢性氧化损伤的防治作用[J].食品科学(LU Zheng,ZHANGJing,GAO Zhao-lan,et al.Protective effect of burdock inulin and Jerusalem artichoke inulin against chronic alcohol-induced oxidative injury in mice[J].Food Science),2010,31(5):270-273.
[7]高桂玲,成家杨,马炯.雨生红球藻和虾青素的研究[J].水产学报(GAO Gui-ling,CHENG Jia-yang,MA Jiong.Research review of Haematococcus pluvialis and astaxathin[J].Journal of Fisheries of China),2014,38(2):297-304.
[8]赵晓燕,朱海涛,毕玉平,等.雨生红球藻中虾青素的研究进展[J].食品研究与开发(ZHAO Xiao-yan,ZHU Hai-tao,BI Yuping,et al.Research of astaxanthin in the Haematococcus pluvialis[J].Food Research and Development),2016,37(4):191-194,195.
[9]孟春晓,高政权,王依涛,等.雨生红球藻中虾青素提取方法研究现状[J].水产科学(MENG Chun-xiao,GAO Zheng-quan,WANG Yi-tao,et al.A review:methods of astaxanthin extraction from alga Haematococcus pluvialis[J].Fisheries Science),2010,29(12):745-748.
[10]黄浦俊,朱巧巧,颜美秋,等.破壁、未破壁雨生红球藻粉体外抗氧化活性及体内抗衰老作用及比较研究[J].中药药理与临床(HUANG Pu-jun,ZHU Qiao-qiao,YAN Mei-qiu,et al.Study on and comparison antioxidant activities in vitro and antiaging effects in vivo between Haematococcus pluvialis powders and broken Haeatococcus pluvialis powders[J].Pharmacology and Clinics of Chinese Materia Medica),2018,34(2):83-87.
[11]刘颖芬,辛乃宏,李炳乾,等.雨生红球藻虾青素对小鼠免疫调节的研究[J].食品研究与开发(LIU Ying-fen,XIN Nai-hong,LI Bing-qian,et al.Study of astaxanthin from Haematococcus pluvialis on immune regulation function in mice[J].Food Research and Development),2017,38(20):183-187.
[12]李勇超,贺青华,刘瑞雪,等.雨生红球藻源虾青素对糖尿病小鼠的降糖作用及其机制[J].食品工业科技(LI Yong-chao,HE Qing-hua,LIU Rui-xue,et al.Effect of Haematococcus pluvialis astaxanthin on diabetes mice for decreasing blood glucose and its mechanisms[J].Science and Technology of Food Industry),2016,37(24):355-359.
[13]郭艳,车茜,刘科亮.雨生红球藻软胶囊对小鼠的增强免疫力功能实验研究[J].食品与发酵科技(GUO Yan,CHE Qian,LIU Ke-liang.Immunological function of Haematococcus pluvialis soft capsule on mice[J].Food and Fermentation Technology),2016,52(3):33-36
[14]魏芬芬,王文娟,贺青华,等.雨生红球藻破壁孢子粉对酒精性肝损伤的保护作用研究[J].食品工业科技(WEI Fen-fen,WANG Wen-juan,HE Qing-hua,et al.Study on the protective effect of Haematococcus pluvialis on alcoholic liver injury[J].Science and Technology of Food Industry),2019,40(5):270-274.
[15]GARAYCOECHEA J I,CROSSAN G P,LANGEVIN F,et al.Alcohol and endogenous aldehydes damage chromosomes and mutate stem cells[J].Nature,2018,553(7687):171-177.
[16]ROSWALL N,WEIDERPASS E.Alcohol as a risk factor for cancer:existing evidence in a global perspective[J].Journal of Preventive Medicine and Public Health,2015,48(1):1-9.
[17]李亚琳,权美平,陆苗.四氯化碳联合二乙基亚硝胺对小鼠肾脏毒性作用的研究[J].生命科学研究(LI Ya-lin,QUAN Meiping,LU Miao.Study on the toxicity of carbon tetrachloride combining with diethylnitrosamine to mouse kidney tissue[J].Life Science Research,2017,21(1):6-9.
[18]刘莹莹,武俊紫,李伟,等.金钗石斛多糖对2型糖尿病大鼠心肌组织凋亡相关蛋白的影响[J].中西医结合心脑血管病杂志(LIU Ying-ying,WU Jun-zi,LI Wei,et al.Effects of Dendrobium nobile polysaccharides on apoptosis related proteins of alleviates myocardium in type 2 diabetic rats[J].Chinese Journal of Integrative Medicine on Cardio-Cerebrovascular Disease),2018,16(18):2621-2626.
[19]齐玉增,苟欣,周青松,等.骨髓间充质干细胞移植在小鼠急性肾损伤肾内的存活与分化情况研究[J].生命科学研究(QIYu-zeng,GOU Xin,ZHOU Qing-song,et al.Survival and differentiation of transplanted bone marrow mesenchymal stem cells in kidneys of acute renal injury mice[J].Life Science Research),2011,15(6):471-475.
[20]王楠,蔡夏夏,鲍雷,等.膳食5′-核苷酸对大鼠酒精性肾损伤保护作用的研究[J].科技导报(WANG Nan,CAI Xia-xia,BAO Lei,et al.Protective effect of dietary 5′-nucleotides on alcohol-induced kidney injury of mice[J].Science and Technology Review),2017,35(10):64-70.
[21]CHOI S H,LEE A Y,PARK C H,et al.Protective effect of Carthamus tinctorius L.seed on oxidative stress and cognitive impairment induced by chronic alcohol consumption in mice[J].Food Science and Biotechnology,2018,27(5):1475-1484.
[22]WU B Y,ZHU M,RUAN T,et al.Oxidative stress,apoptosis and abnormal expression of apoptotic protein and gene and cell cycle arrest in the cecal tonsil of broilers induces by dietary methionine deficiency[J].Research in Veterinary Science,2018,121:65-75.
[23]WU F,HUANG W,LIU Q,et al.Responses of antioxidant defense and immune gene expression in early life stages of large yellow croaker(Pseudosciaena crocea)under methyl mercury exposure[J].Frontiers in Physiology,2018,DOI:10.3389/fphys.2018.01436.
[24]王潮岗,韩燊,陈甄倩,等.雨生红球藻抗氧化系统对活性氧的清除机制[J].水生生物学报(WANG Chao-gang,HAN Shen,CHEN Zhen-qian,et al.The scavenging of reactive oxygen species with antioxidant systems in Haematococcus pluvialis[J].Acta Hydrobiologica Sinica),2012,36(4):804-808.
[25]YIN Y,LIU X,LIU J,et al.Beta-sitosterol and its derivatives repress lipopolysaccharide/D-galactosamine-induced acute hepatic injury by inhibiting the oxidation and inflammation in mice[J].Bioorganic&Medicinal Chemistry Letters,2018,28(9):1525-1533.