γ-谷氨酰转肽酶基因工程菌的研究进展
|
摘要
谷氨酰转肽酶是一种生物体内非常关键的酶,普遍存在于微生物、和动植物体内,能够特异性催化谷氨酰类化合物的合成,对氨基酸的转运过程有着不可替代的作用。γ-谷氨酰转肽酶广泛运用于临床诊断、食品工业、催化合成药物等行业。由于γ-谷氨酰转肽酶的重要性,研究者们对该酶的相关性质和实践应用日益重视。本文主要对γ-谷氨酰转肽酶性质和作用机理以及固定化技术和应用进行了综述。
Gamma-glutamyl transpeptidase is a very key enzyme in the biological body,which is widely found in microorganisms,animals and plants. It can specifically catalyze the synthesis of glutamyl compounds and plays an irreplaceable role in the transport process of amino acids. Gamma-glutamyl transpeptidase is widely used in clinical diagnosis,food industry,catalytic synthesis of drugs and other industries. Due to the importance of gamma-glutamyl transpeptidase,researchers pay more and more attention to the related properties and practical applications of gamma-glutamyl transpeptidase. In this paper,the nature and mechanism of gamma-glutamyl transpeptidase and the immobilization technology and application of gamma-glutamyl transpeptidase were reviewed.
Gamma-glutamyl transpeptidase is a very key enzyme in the biological body,which is widely found in microorganisms,animals and plants. It can specifically catalyze the synthesis of glutamyl compounds and plays an irreplaceable role in the transport process of amino acids. Gamma-glutamyl transpeptidase is widely used in clinical diagnosis,food industry,catalytic synthesis of drugs and other industries. Due to the importance of gamma-glutamyl transpeptidase,researchers pay more and more attention to the related properties and practical applications of gamma-glutamyl transpeptidase. In this paper,the nature and mechanism of gamma-glutamyl transpeptidase and the immobilization technology and application of gamma-glutamyl transpeptidase were reviewed.
引文
[1]Meister Alton,Suresh S Tate,Owen W G.Eriffitr.γ-lutamyltranspeptidase[J].Methods in Enzymology,1981,77:237-253.
[2]Ogawa Y,Hosoyama H,Hamano M,et al.Purification and properties of gamma-glutamyl-transpeptidase from Bacillus subtilis(natto)[J].Agricultural and Biological Chemistry,1991,55(12):2971-2977.
[3]Minami H,Suzuki H.Salt-tolerant gammaglutamyltranspeptidase from Bacillus subtilis 168 with glutaminase activity[J].Enzyme and Microbial Technology,2003,32(3):431-438.
[4]Abe K,Ito Y,Ohmachi T,et al.Purification and properties of two isozymes of gamma-glutamyltranspeptidase from Bacillus subtilis TAM-4[J].Bioscience Biotechnology and Biochemistry,1997,61(10):1621-1625.
[5]Roselyne C,Dany H K.Kinetic characterization and identification of the acylation and glycosylation sites of recombinant humanγ-glutamyltranspeptidase[J].Biochemistry,2007,46(43):12253-12262.
[6]Toshihiro O,Hideyuki S.Crystal structure of theγ-glutamyltranspeptidase precursor protein from E.coli:Structural changes upon autocatalytic processing and implications for the maturation mechanism[J].J Biom Chen,2007,282:2433-2439.
[7]Wataru H,Hideyuki S,Sttoko N,et al.Subunit association ofγ-glutamyltranspeptidase of Escherichia coli K-12[J].J.Biochem,1995,118(6):1216-1223.
[8]Keillor J W,Castonguay R,Lherbet C.Gammaglutamyltranspeptidase substrate specificity anf catalytic mechanism[J].Method Enzymol,2005,401:449-467.
[9]Suzuki H,Yamada C,Kato K.Gamma-Glutamy compounds and their enzymatic production using bacterial gammaglutamyltrainspeptidase[J].Amino Acids,2007,32(3):333-340.
[10]苟志金,贺忠,张征,等.γ-谷氨酰转肽酶及其应用[J].南京工业大学学报,2004,26(4):106-110.
[11]Suzuki H,Izuka S,Miyakawa N,et al.Enzymatic production of the anine,an"umami"component of tea,from glutamine and ethylamine with bacterial gamma-glutamyltranspeptidase[J].Enzyme and Microbial Technology,2002,31(6):884-889.
[12]Suzuki H,Miyakawa N,Kumagai H,et al.Enzymatic production ofγ-L-glutamyltaurine through the transpeptidation reaction ofγ-glutamyltranspeptidase from E.coli K-12[J].Enzyme Microd.Tech,2002,30(7):883-888.
[13]Suzuki H,Kato K,Kumagai H.Development of an efficient enzymatic production of D-glutamyl-L-tryptophan(SCV-07),a prospective medicine for tuberculosis,with bacterialγ-glutamyltranspeptidase[J].J Biotechnol,2004,111(3):291-295.
[14]Suzuki H,Izuka S,Minami H,et al.Use of bacterialγ-glutamyltranspeptidase for enzymatic synthesis ofγ-D-glutamyl compounds[J].Appl Environ Microb,2003,69(11):6399-6404.
[15]Bridge W J,Zarka M H.Process for the production ofγ-glutamlcysteine:US,11/887 667[P].2012-07-08.
[16]张琛,张宽朝,章琛.海藻酸钠包埋法制备固定化菠萝蛋白酶[J].生物加工过程,2009(6):31-34.
[17]李向阳,单守水,徐世艾.固定化真菌α-淀粉酶在海参饵料添加剂方面的应用研究[J].烟台大学学报(自然科学与工程版),2011,24(4):275-280.
[18]Chibata I,Tosa T,Sato T,et al.Immobilized aspartasecontaining microbial cells:preparation and enzymatic properties[J].Applied Microbiology,1974,27(5):878-885.
[19]曹泽虹,秦卫东,李超,等.响应面法优化内切型菊粉酶发酵生产培养基[J].食品科学,2013,34(13):186-192.
[20]Boanca G,Sand A,Barycki J J.Uncoupling the enzymatic and autoprocessing activities of Helicobacter pylori gammaglutamyltranspeptidase[J].Joumal of Biological Chemistry,2006,281(28):19029-19037.
[21]Suzuki H,Kumagai H.Autocatalytic processing of gammaglutamyltranspeptidase[J].The Joumal of Biological Chemistry,2002,277(45):43536-43543.
[22]Kinlough C L,Poland P A,Bruns J B,et al.Gammaglutamyltranspeptidase:Disulfide bridges,propeptide cleavage,and activation in the endoplasmic reticulum[J].Gluthione Transferases and Gamma-Glutamyl Transpe ptidases,2005,401:426-449.
[23]Meister Alton,Suresh S Tate,Owen W G eriffitr.-glutamyltranspeptidase[J].Methods in Enzymology,1981,77:237-253.
[24]蒋敏丽,孟佩佩,高鹏,等.γ-谷氨酰转肽酶酶学性质的研究[J].广东药学院学报,2010,26(5):529-532.
[25]Wada K,Irie M,Suzuki H,et al.Crystal structure of the halotolerant gamma-glutamyltranspeptidase from Bacillus subtilis in complex with glutamate reveals a unique architecture of the solvent-exposed catalytic pocket[J].Febs Jourmal,2010,277(4):1000-1009.
[26]李伟桥,江波.γ-谷氨酰转肽酶的发酵生产及酶法合成γ-谷氨酰甲胺的研究[D].无锡:江南大学,2011.
[27]Castellano,I.&Merlino,A.γ-Glutamyltranspeptidase:sequence,structure,biochemical properties,and biotechnological applications[J].Cellular and Molecular Life Sciences,2012,69(20):3381-3394.
[28]刘存飞,刘承云.谷氨酰转肽酶与代谢综合征的关系研究进展[J].临床心血管病杂志,2011,27(4):246-248.
[29]Suzuki H,Kumagai H,Tochikura T,.GammaGlutamyltranspeptidase from Escherichia coli K-12:formation and localization[J].J Bacteriol,1986,168(3):1332-1335.
[30]Suzuki H,Kumagai H.Autocatalytic processing of gammaglutamyltranspeptidase.J Biol Chem,2002,277(45):43536-43.
[31]Okada T,Suzuki H,Wada K,et al.Crystal structure of the gamma-glutamyltranspeptidase precursor protein from Escherichia coli.Structural changes upon autocatalytic processing and implications for the maturation mechanism[J].J Biol Chem,2007,282(4):2433-9.
[32]蒋毅,殷志敏.具有极高谷氨酰转肽酶活性的大肠杆菌基因工程菌的构建并利用它高效酶促合成茶氨酸[D].南京:南京师范大学,2014.
[33]刘佳,张蕾,申刚义.石墨烯固定化酶研究进展[J].化学通报,2017,80(1):41-46.
[34]蒋敏丽,孟佩佩,高鹏,等.γ-谷氨酰转肽酶酶学性质的研究[J].广东药学院学报,2010,26(5):529-532.
[35]韦敏,姚忠,叶丽静,等.载体两性电解质修饰对固定化γ-谷氨酰转肽酶p H耐受性和催化活性的影响[J].生物加工过程,2016,14(4):37-42.
[36]秦丹华,姚忠,王浩琦,等.介孔二氧化钛固载γ-谷氨酰转肽酶的制备及性质[J].化工学报,2011,62(2):378-385.
[37]肖彦羚,周治,姚忠,等.固定化γ-谷氨酰转肽酶的催化特性研究[J].现代化工,2009,29(2):147-151.
[38]宋希文,周治,姚忠,等.固定化γ-谷氨酰转肽酶催化合成γ-L-谷氨酰-L-半胱氨酸[J].化学反应工程与工艺,2009,25(5):240-245.
[39]李旭,张振华,蔡群,等.谷氨酰转肽酶/HBs Ag比值评估慢性乙型肝炎患者肝纤维化的价值探讨[J].实用肝脏病杂志,2015(2):141-144.
[40]Ousilahti P,Rastenyte D,Tuomilehto J.Serum gammaglutamyltransferase self-reported alcohol drinking,and the risk of stroke[J].Stroke,2000,31:1851-1855.
[41]Michele E,Claudio P,Alfonso P,et al.Serum gammaglutamyltranspeptidase:Aprognostic marker in cardiovascular diseases[J].Bio Factors,2003,(17):199-205.
[42]Okan T,Ahmet Y,Kenan Y,et al.γ-Glutamyltransferase is a promising biomarker for cardiovascular risk[J].Medical Hypotheses,2006(67):1060-1064.
[43]张威.血清γ-谷氨酰胺转肽酶(GGT)与初诊2型糖尿病患者胰岛素抵抗等相关性分析[D].长春:吉林大学,2014.
[44]王梦婷,徐礼生,柴存宝,等.谷氨酰转肽酶基因工程菌发酵条件优化的研究[J].山东化工,2018,47(5):17-19.
[2]Ogawa Y,Hosoyama H,Hamano M,et al.Purification and properties of gamma-glutamyl-transpeptidase from Bacillus subtilis(natto)[J].Agricultural and Biological Chemistry,1991,55(12):2971-2977.
[3]Minami H,Suzuki H.Salt-tolerant gammaglutamyltranspeptidase from Bacillus subtilis 168 with glutaminase activity[J].Enzyme and Microbial Technology,2003,32(3):431-438.
[4]Abe K,Ito Y,Ohmachi T,et al.Purification and properties of two isozymes of gamma-glutamyltranspeptidase from Bacillus subtilis TAM-4[J].Bioscience Biotechnology and Biochemistry,1997,61(10):1621-1625.
[5]Roselyne C,Dany H K.Kinetic characterization and identification of the acylation and glycosylation sites of recombinant humanγ-glutamyltranspeptidase[J].Biochemistry,2007,46(43):12253-12262.
[6]Toshihiro O,Hideyuki S.Crystal structure of theγ-glutamyltranspeptidase precursor protein from E.coli:Structural changes upon autocatalytic processing and implications for the maturation mechanism[J].J Biom Chen,2007,282:2433-2439.
[7]Wataru H,Hideyuki S,Sttoko N,et al.Subunit association ofγ-glutamyltranspeptidase of Escherichia coli K-12[J].J.Biochem,1995,118(6):1216-1223.
[8]Keillor J W,Castonguay R,Lherbet C.Gammaglutamyltranspeptidase substrate specificity anf catalytic mechanism[J].Method Enzymol,2005,401:449-467.
[9]Suzuki H,Yamada C,Kato K.Gamma-Glutamy compounds and their enzymatic production using bacterial gammaglutamyltrainspeptidase[J].Amino Acids,2007,32(3):333-340.
[10]苟志金,贺忠,张征,等.γ-谷氨酰转肽酶及其应用[J].南京工业大学学报,2004,26(4):106-110.
[11]Suzuki H,Izuka S,Miyakawa N,et al.Enzymatic production of the anine,an"umami"component of tea,from glutamine and ethylamine with bacterial gamma-glutamyltranspeptidase[J].Enzyme and Microbial Technology,2002,31(6):884-889.
[12]Suzuki H,Miyakawa N,Kumagai H,et al.Enzymatic production ofγ-L-glutamyltaurine through the transpeptidation reaction ofγ-glutamyltranspeptidase from E.coli K-12[J].Enzyme Microd.Tech,2002,30(7):883-888.
[13]Suzuki H,Kato K,Kumagai H.Development of an efficient enzymatic production of D-glutamyl-L-tryptophan(SCV-07),a prospective medicine for tuberculosis,with bacterialγ-glutamyltranspeptidase[J].J Biotechnol,2004,111(3):291-295.
[14]Suzuki H,Izuka S,Minami H,et al.Use of bacterialγ-glutamyltranspeptidase for enzymatic synthesis ofγ-D-glutamyl compounds[J].Appl Environ Microb,2003,69(11):6399-6404.
[15]Bridge W J,Zarka M H.Process for the production ofγ-glutamlcysteine:US,11/887 667[P].2012-07-08.
[16]张琛,张宽朝,章琛.海藻酸钠包埋法制备固定化菠萝蛋白酶[J].生物加工过程,2009(6):31-34.
[17]李向阳,单守水,徐世艾.固定化真菌α-淀粉酶在海参饵料添加剂方面的应用研究[J].烟台大学学报(自然科学与工程版),2011,24(4):275-280.
[18]Chibata I,Tosa T,Sato T,et al.Immobilized aspartasecontaining microbial cells:preparation and enzymatic properties[J].Applied Microbiology,1974,27(5):878-885.
[19]曹泽虹,秦卫东,李超,等.响应面法优化内切型菊粉酶发酵生产培养基[J].食品科学,2013,34(13):186-192.
[20]Boanca G,Sand A,Barycki J J.Uncoupling the enzymatic and autoprocessing activities of Helicobacter pylori gammaglutamyltranspeptidase[J].Joumal of Biological Chemistry,2006,281(28):19029-19037.
[21]Suzuki H,Kumagai H.Autocatalytic processing of gammaglutamyltranspeptidase[J].The Joumal of Biological Chemistry,2002,277(45):43536-43543.
[22]Kinlough C L,Poland P A,Bruns J B,et al.Gammaglutamyltranspeptidase:Disulfide bridges,propeptide cleavage,and activation in the endoplasmic reticulum[J].Gluthione Transferases and Gamma-Glutamyl Transpe ptidases,2005,401:426-449.
[23]Meister Alton,Suresh S Tate,Owen W G eriffitr.-glutamyltranspeptidase[J].Methods in Enzymology,1981,77:237-253.
[24]蒋敏丽,孟佩佩,高鹏,等.γ-谷氨酰转肽酶酶学性质的研究[J].广东药学院学报,2010,26(5):529-532.
[25]Wada K,Irie M,Suzuki H,et al.Crystal structure of the halotolerant gamma-glutamyltranspeptidase from Bacillus subtilis in complex with glutamate reveals a unique architecture of the solvent-exposed catalytic pocket[J].Febs Jourmal,2010,277(4):1000-1009.
[26]李伟桥,江波.γ-谷氨酰转肽酶的发酵生产及酶法合成γ-谷氨酰甲胺的研究[D].无锡:江南大学,2011.
[27]Castellano,I.&Merlino,A.γ-Glutamyltranspeptidase:sequence,structure,biochemical properties,and biotechnological applications[J].Cellular and Molecular Life Sciences,2012,69(20):3381-3394.
[28]刘存飞,刘承云.谷氨酰转肽酶与代谢综合征的关系研究进展[J].临床心血管病杂志,2011,27(4):246-248.
[29]Suzuki H,Kumagai H,Tochikura T,.GammaGlutamyltranspeptidase from Escherichia coli K-12:formation and localization[J].J Bacteriol,1986,168(3):1332-1335.
[30]Suzuki H,Kumagai H.Autocatalytic processing of gammaglutamyltranspeptidase.J Biol Chem,2002,277(45):43536-43.
[31]Okada T,Suzuki H,Wada K,et al.Crystal structure of the gamma-glutamyltranspeptidase precursor protein from Escherichia coli.Structural changes upon autocatalytic processing and implications for the maturation mechanism[J].J Biol Chem,2007,282(4):2433-9.
[32]蒋毅,殷志敏.具有极高谷氨酰转肽酶活性的大肠杆菌基因工程菌的构建并利用它高效酶促合成茶氨酸[D].南京:南京师范大学,2014.
[33]刘佳,张蕾,申刚义.石墨烯固定化酶研究进展[J].化学通报,2017,80(1):41-46.
[34]蒋敏丽,孟佩佩,高鹏,等.γ-谷氨酰转肽酶酶学性质的研究[J].广东药学院学报,2010,26(5):529-532.
[35]韦敏,姚忠,叶丽静,等.载体两性电解质修饰对固定化γ-谷氨酰转肽酶p H耐受性和催化活性的影响[J].生物加工过程,2016,14(4):37-42.
[36]秦丹华,姚忠,王浩琦,等.介孔二氧化钛固载γ-谷氨酰转肽酶的制备及性质[J].化工学报,2011,62(2):378-385.
[37]肖彦羚,周治,姚忠,等.固定化γ-谷氨酰转肽酶的催化特性研究[J].现代化工,2009,29(2):147-151.
[38]宋希文,周治,姚忠,等.固定化γ-谷氨酰转肽酶催化合成γ-L-谷氨酰-L-半胱氨酸[J].化学反应工程与工艺,2009,25(5):240-245.
[39]李旭,张振华,蔡群,等.谷氨酰转肽酶/HBs Ag比值评估慢性乙型肝炎患者肝纤维化的价值探讨[J].实用肝脏病杂志,2015(2):141-144.
[40]Ousilahti P,Rastenyte D,Tuomilehto J.Serum gammaglutamyltransferase self-reported alcohol drinking,and the risk of stroke[J].Stroke,2000,31:1851-1855.
[41]Michele E,Claudio P,Alfonso P,et al.Serum gammaglutamyltranspeptidase:Aprognostic marker in cardiovascular diseases[J].Bio Factors,2003,(17):199-205.
[42]Okan T,Ahmet Y,Kenan Y,et al.γ-Glutamyltransferase is a promising biomarker for cardiovascular risk[J].Medical Hypotheses,2006(67):1060-1064.
[43]张威.血清γ-谷氨酰胺转肽酶(GGT)与初诊2型糖尿病患者胰岛素抵抗等相关性分析[D].长春:吉林大学,2014.
[44]王梦婷,徐礼生,柴存宝,等.谷氨酰转肽酶基因工程菌发酵条件优化的研究[J].山东化工,2018,47(5):17-19.