生地黄汤对卵巢早衰大鼠骨代谢的干预作用
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摘要
目的研究生地黄汤对卵巢早衰大鼠骨代谢的影响。方法 48只成年雌性SD大鼠,随机分为空白组、模型组、阳性对照组、生地黄汤高、中、低剂量治疗组。除空白组外,其余各组每日灌胃雷公藤多甙片75 mg/kg,连续造模14 d。从第15 d起,模型组灌胃生理盐水2 mL/kg;阳性对照组灌胃补佳乐1 mg/kg;高、中、低剂量治疗组灌胃生地黄汤(12、6、3 g/kg),每日1次,连续给药21天。放射免疫法测定血清中雌激素(E2)、孕酮(P)的含量;比色法检测血清中钙、磷的含量;ELISA法检测血清中碱性磷酸酶(ALP)、骨碱性磷酸酶(BALP)、1,25二羟基维生素D_3(1,25(OH)_2D_3)含量。结果与模型组比较,生地黄汤高、中剂量治疗组血清中E2、P水平升高(P<0.05);生地黄汤高剂量组血清钙、磷的含量高于模型组(P<0.05);生地黄汤各剂量组血清ALP、BALP含量均较模型组降低(P<0.05),1,25(OH)2D3含量高于模型组(P<0.01)。结论生地黄汤可以通过类雌激素样作用升高血清雌二醇水平,改善早衰卵巢功能,同时增强成骨细胞活性,使骨形成大于骨吸收,调节骨代谢平衡,改善卵巢功能减退引起骨质疏松。
Objective To investigate the protective effect of raw radix rehmanniae decoction( RRRD) on bone metabolism in rats with premature ovarian failure. Methods A total of 48 adult female SD rats were randomly divided into control group,model group,positive control group,and high,medium,or low dose RRRD group. Except in control group,rats in other groups received tripterygium glycosides for 14 d to set up model of premature ovarian failure. From the 15 d,rats in model group received 2 mL/kg of normal saline,rats in positive control group received 1 mg/kg of Bujiale,and rats in high,medium,or low dose RRRD group received 12,6,or 3 g/kg of raw radix rehmanniae decoction,respectively,once a day for 21 days. The content of E2 and P was detected with radioimmunoassay. Serum calcium and phosphorus were determined with colorimetric method. Serum ALP,BALP,and 1, 25(OH)_2D_3 were determined with ELISA. Results Compared with those in the model group,the serum levels of E2 and P in the high and middle dose treatment group of RRRD increased( P < 0. 05). The serum contents of Ca~(2+) and phosphorus in the high dose group of RRRD were higher than those in the model group( P < 0. 05). The contents of ALP and BALP in the RRRD groups were lower than those in the model group( P < 0. 05). The content of 1, 25(OH)_2D_3 in the SDHD group was higher than that in the model group( P < 0. 01). Conclusion RRRD increases serum estradiol level and enhance osteoblast activity in premature ovarian function,improves bone formation over bone resorption,regulates bone metabolism balance,and relieves osteoporosis caused by ovarian dysfunction.
Objective To investigate the protective effect of raw radix rehmanniae decoction( RRRD) on bone metabolism in rats with premature ovarian failure. Methods A total of 48 adult female SD rats were randomly divided into control group,model group,positive control group,and high,medium,or low dose RRRD group. Except in control group,rats in other groups received tripterygium glycosides for 14 d to set up model of premature ovarian failure. From the 15 d,rats in model group received 2 mL/kg of normal saline,rats in positive control group received 1 mg/kg of Bujiale,and rats in high,medium,or low dose RRRD group received 12,6,or 3 g/kg of raw radix rehmanniae decoction,respectively,once a day for 21 days. The content of E2 and P was detected with radioimmunoassay. Serum calcium and phosphorus were determined with colorimetric method. Serum ALP,BALP,and 1, 25(OH)_2D_3 were determined with ELISA. Results Compared with those in the model group,the serum levels of E2 and P in the high and middle dose treatment group of RRRD increased( P < 0. 05). The serum contents of Ca~(2+) and phosphorus in the high dose group of RRRD were higher than those in the model group( P < 0. 05). The contents of ALP and BALP in the RRRD groups were lower than those in the model group( P < 0. 05). The content of 1, 25(OH)_2D_3 in the SDHD group was higher than that in the model group( P < 0. 01). Conclusion RRRD increases serum estradiol level and enhance osteoblast activity in premature ovarian function,improves bone formation over bone resorption,regulates bone metabolism balance,and relieves osteoporosis caused by ovarian dysfunction.
引文
[1]骆瑶,陈兰英,官紫祎,等.杜仲提取物对去卵巢骨质疏松大鼠骨代谢、骨密度及骨微结构的影响[J].中药材,2016,39(11):2624-2628.
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[9]王东生,韩婧,康文博,等.植物雌激素防治骨质疏松作用的机制进展[J].中国骨质疏松杂志,2016,22(5):632-639.
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[11]朱刚,孙海斌,徐刚.肉苁蓉醇提物对去卵巢骨质疏松大鼠的治疗作用及其机制[J].吉林大学学报(医学版),2018,44(1):68-72.
[12]Zhao D,WangJ,Liu Y,et al.Expressions and clinical significance of serum bone Gla-protein,bone alkaline phosphatase and Cterminal telopeptide of typeⅠcollagen in bone metabolism of patients with osteoporosis[J].Pak J Med Sci,2015,31(1):91-94.
[13]刘红,廖二元,伍贤平,等.正常女性与年龄相关的骨转换生化指标和骨密度的关系[J].中华内科杂志,2004,43(11):805.
[14]Bettica P,Bevilacqua M,Vago T,et al.High prevalence of hypovitaminosis D among free living postmenopausal women referred to an osteoporosis outpatient clinic in northern Italy for initial screening.Osteoporos Int,1999,9(3):226-229.
[15]李玲玲,刘蔚霞.补肾疏肝方对卵巢早衰患者骨代谢及免疫功能的影响[J].新中医,2017,49(9):81-85.
[16]唐娅辉,刘学武,刘锐,等.强骨生血口服液对去卵巢致骨质疏松大鼠的治疗作用及其机制[J].中草药,2017,48(23):4935-4910.(收稿日期:02;修回日期:30)
[2]刘爽,张玉莲,毕聪聪,等.补肾法中药对去卵巢大鼠骨质疏松骨代谢指标的影响[J].中国老年学杂志,2017,37(2):529-530.
[3]郑晓珂,刘朝妍,蒋贇,等.怀地黄雌激素样活性筛选的实验研究[J].中国药学杂志,2013,48(21):1831-1836.
[4]卢宁,张树峰,佟继铭,等.大黄水提物对成年大鼠子宫及卵巢的影响[J].中国实验方剂杂志,2013,19(21):258-261.
[5]马淑月,张树峰,佟继铭,等.大黄不同萃取物对大鼠子宫卵巢形态结构及血清中P、T、E2激素水平的影响[J].中药药理与临床,2017,33(3):91-94.
[6]郝娟,王春莲,王培嵩,等.雷公藤多甙片致卵巢早衰大鼠动物模型的研究[J].中国妇幼保健,2012,27(12):1866-1870.
[7]薛志远,赵良功,师志强,等.红芪和黄芪对去卵巢致大鼠骨质疏松防治作用的研究[J].现代中医研究与实践,2017,31(6):21-24.
[8]赵素霞,刘会丽,江红.菟丝子黄酮通过调节Wnt/β-catenin信号通路干预去卵巢大鼠骨代谢的机制研究[J].临床和实验医学杂志,2018,17(1):25-28.
[9]王东生,韩婧,康文博,等.植物雌激素防治骨质疏松作用的机制进展[J].中国骨质疏松杂志,2016,22(5):632-639.
[10]邹仲之,李继承.组织学与胚胎学[M].北京:人民卫生出版,2008:44-47.
[11]朱刚,孙海斌,徐刚.肉苁蓉醇提物对去卵巢骨质疏松大鼠的治疗作用及其机制[J].吉林大学学报(医学版),2018,44(1):68-72.
[12]Zhao D,WangJ,Liu Y,et al.Expressions and clinical significance of serum bone Gla-protein,bone alkaline phosphatase and Cterminal telopeptide of typeⅠcollagen in bone metabolism of patients with osteoporosis[J].Pak J Med Sci,2015,31(1):91-94.
[13]刘红,廖二元,伍贤平,等.正常女性与年龄相关的骨转换生化指标和骨密度的关系[J].中华内科杂志,2004,43(11):805.
[14]Bettica P,Bevilacqua M,Vago T,et al.High prevalence of hypovitaminosis D among free living postmenopausal women referred to an osteoporosis outpatient clinic in northern Italy for initial screening.Osteoporos Int,1999,9(3):226-229.
[15]李玲玲,刘蔚霞.补肾疏肝方对卵巢早衰患者骨代谢及免疫功能的影响[J].新中医,2017,49(9):81-85.
[16]唐娅辉,刘学武,刘锐,等.强骨生血口服液对去卵巢致骨质疏松大鼠的治疗作用及其机制[J].中草药,2017,48(23):4935-4910.(收稿日期:02;修回日期:30)