正交法优化ZnO/MMT抗菌材料制备条件及抑菌活性研究
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摘要
以氯化锌、氢氧化钠为原料,蒙脱石(Montmorillonite,MMT)为载体,最小抑菌浓度(MIC)和最小杀菌浓度(MBC)为测试指标,采用正交试验考察物料配比、反应温度、反应时间等因素对该抗菌材料抑菌活性的影响,确定ZnO/MMT抗菌材料的最佳制备条件,并通过XRD、SEM和BET对其结构进行了表征。结果表明,影响该抗菌材料性能的因素依次为反应温度>物料配比>反应时间,制备ZnO/MMT抗菌材料的最佳条件为氯化锌:氢氧化钠=1:8,反应温度为70℃,反应时间为18 h。ZnO/MMT抗菌材料对沙门氏菌(Salmonella)、大肠杆菌(E.coli)和金黄色葡萄球菌(S.aureus)具有广谱抗菌性。
The antibacterial material ZnO/MMT was synthesized with zinc chloride and sodium hydroxide as raw materials and montmorillonite( MMT) as carrier,which was characterized by XRD,SEM and BET. The optimum preparation conditions of ZnO/MMT,such as material ratio,reaction temperature and reaction time,were investigated by orthogonal test with the indexes of minimum inhibition concentration( MIC) and minimum bactericidal concentration( MBC). The results showed that the sequences of influencing factors are reaction temperature > material ratio > reaction time. The optimal preparation conditions of ZnO/MMT are as follows,zinc chloride:sodium hydroxide = 1:8,the reaction temperature at 70 ℃ and the reaction time for 18 h. What's more,ZnO/MMT has a broad spectrum of antimicrobial activity against Salmonella,E.coli and S.aureus.
The antibacterial material ZnO/MMT was synthesized with zinc chloride and sodium hydroxide as raw materials and montmorillonite( MMT) as carrier,which was characterized by XRD,SEM and BET. The optimum preparation conditions of ZnO/MMT,such as material ratio,reaction temperature and reaction time,were investigated by orthogonal test with the indexes of minimum inhibition concentration( MIC) and minimum bactericidal concentration( MBC). The results showed that the sequences of influencing factors are reaction temperature > material ratio > reaction time. The optimal preparation conditions of ZnO/MMT are as follows,zinc chloride:sodium hydroxide = 1:8,the reaction temperature at 70 ℃ and the reaction time for 18 h. What's more,ZnO/MMT has a broad spectrum of antimicrobial activity against Salmonella,E.coli and S.aureus.
引文
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[2] Jennings E,Thurston T L M,Holden D W. Salmonella SPI-2 Type Ⅲ Secretion System Effectors:Molecular Mechanisms And Physiological Consequences[J]. Cell Host&Microbe,2017,22(2):217-231.
[3]郝喜海,孙淼,邓靖.抗菌材料的研究进展[J].化工技术与开发,2011,40(9):21-24.
[4] Pandimurugan R,Thambidurai S. Novel seaweed capped ZnO nanoparticles for effective dye photodegradation and antibacterial activity[J].Advanced Powder Technology,2016,27(4):1062-1072.
[5]麻晓霞,裴阳阳,雷云,等.负载型无机抗菌材料的研究进展[J].功能材料,2017,9(48):09038-09042.
[6] Jayrajsinh S,Shankar G,Agrawal Y K,et al. Montmorillonite nanoclay as a multifaceted drug-delivery carrier:A review[J]. Journal of Drug Delivery Science and Technology,2017,39:200-209.
[7]陈继发,康克浪,曲湘勇.蒙脱石的作用机制及其在家禽生产中的应用[J].动物营养学报,2018,30(4):1-7.
[8]唐佳丽,徐章韬.正交试验中的极差分析与方差分析[J].中学数学,2017,9:31-34.
[9] Boccard J,Rudaz S. Exploring Omics data from designed experiments using analysis of variance multiblock Orthogonal Partial Least Squares[J].Analytica Chimica Acta,2016,920:18-28.
[10]吴晓松,褚学宁,李玉鹏.基于正交试验的PECVD法沉积氮化硅薄膜工艺参数优化研究[J].人工晶体学报,2014,43(8):1913-1920.
[11] Gu N,Gao J L,Wang K,et al. ZnO-montmorillonite as photocatalyst and flocculant for inhibition of cyanobacterial bloom[J]. Water Air Soil Poll,2015,226(5):1-12.
[12]麻晓霞,马玉龙.氧化锌型复合抗菌材料抗菌活性研究进展[J].功能材料,2018,49(9):09061-09066.
[13] Siddique S,Shah Z H,Shahid S,et al. Preparation,Characterization and Antibacterial Activity of ZnO Nanoparticles on Broad Spectrum of Microorganisms[J]. Acta Chimica Slovenica,2013,60(3):660-665.
[14] Kumar R,Umar A,Kumar G,et al. Antimicrobial Properties of ZnO Nanomaterials:A Review[J]. Ceramics International,2016,43(5):3940-3961.