平肝脉通片对阴虚阳亢型高血压大鼠一氧化氮、内皮素干预作用的研究
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摘要
目的:探讨平肝脉通片对阴虚阳亢型高血压大鼠一氧化氮(NO)、内皮素(ET)干预作用的研究。方法:采用二肾一夹方法并灌服附子汤制备阴虚阳亢型高血压大鼠模型,选择造模成功的75只大鼠,随机分为空白对照组、卡托普利对照组、平肝脉通片低剂量组、平肝脉通片中剂量组、平肝脉通片高剂量组,每组15只,分别给予2 m L/100g生理盐水、10 mg/m L卡托普利溶液及10、20、40 mg/m L的平肝脉通片溶液灌胃,连续4周,对比观察各组治疗前后尾动脉血压、血清NO、内皮素-1(ET-1)、血管紧张素Ⅱ(AngⅡ)、血浆醛固酮(ALDO)以及心功能指标左室射血分数(EF%)、短轴缩短率(FS%)变化。结果:与治疗前相比,治疗后空白对照组尾动脉血压、ET-1、AngⅡ、ALDO显著升高(P<0.05),NO显著降低(P<0.05),EF%、FS%无明显变化,而其他4组尾动脉血压、ET-1、AngⅡ、ALDO显著降低,NO、EF%、FS%显著升高,差异均有统计学意义(P<0.05),其中平肝脉通片高剂量组、中剂量组治疗后尾动脉血、ALDO显著低于托普利对照组,NO、EF%、FS%显著高于托普利对照组,治疗后平肝脉通片高剂量AngⅡ显著低于托普利对照组,差异均有统计学意义(P<0.05)。结论:平肝脉通片能有效降低阴虚阳亢型高血压大鼠血压,逆转左心室肥厚,应与其能调节血清NO、ET-1、AngⅡ、ALDO有关。
Objective: To explore the effect of nitric oxide,endothelin on the Yin deficiency type hand renal hypertensive rats intervened by Pinggan-Maitong slices. Methods: The deficiency type hand renal hypertensive rats has been made by adopting the method of two kidney one clip and irrigation clothing monkshood; 75 successfully built rats cases were selected,and they were randomly divided into blank control group,captopril group,hepatic vein on low dose group,hepatic vein on middle dose group,hepatic vein on high dose group,with 15 cases in each group. They were only given 2 m L /100 g physiological saline,10 mg / m L concentration of captopril solution,10 mg / m L,20 mg / m L,and 40 mg / m L concentration of hepatic vein through solution to fill the stomach respectively,for four weeks. Tail arterial blood pressure,serum NO,endothelin 1( ET-1),angiotensin Ⅱ( AngⅡ),plasma aldosterone( ALDO) and cardiac function index of left ventricular ejection fraction( EF) %,short axial shortening rate( FS) % between groups before and after treatment were observed. Results: The tail artery blood pressure,ET-1,AngⅡ in blank control group after treatment were significant rise,ALDO,NO significantly reduced,EF%,FS% had no obvious change,while the tail artery blood pressure,ET-1,Ang Ⅱ,ALDO in other four groups were significantly reduced,NO,EF%,FS% were significantly raised,differences were statistically significant( P < 0. 05); The tail artery blood pressure,ALDO in hepatic vein on middle or high dose group were significantly lower than the captopril control group,NO,EF%,FS% were significantly higher than the captopril control group,the AngⅡ in hepatic vein on high dose group was significantly lower than the captopril control group,the differences were statistically significant( P < 0.05). Conclusions: Pinggan-Maitong slice can reduce the blood pressure in yin deficiency type hand hypertension rats,it can reverse left ventricular hypertrophy,which may related to adjusting the serum NO,ET-1,Ang Ⅱ,ALDO.
Objective: To explore the effect of nitric oxide,endothelin on the Yin deficiency type hand renal hypertensive rats intervened by Pinggan-Maitong slices. Methods: The deficiency type hand renal hypertensive rats has been made by adopting the method of two kidney one clip and irrigation clothing monkshood; 75 successfully built rats cases were selected,and they were randomly divided into blank control group,captopril group,hepatic vein on low dose group,hepatic vein on middle dose group,hepatic vein on high dose group,with 15 cases in each group. They were only given 2 m L /100 g physiological saline,10 mg / m L concentration of captopril solution,10 mg / m L,20 mg / m L,and 40 mg / m L concentration of hepatic vein through solution to fill the stomach respectively,for four weeks. Tail arterial blood pressure,serum NO,endothelin 1( ET-1),angiotensin Ⅱ( AngⅡ),plasma aldosterone( ALDO) and cardiac function index of left ventricular ejection fraction( EF) %,short axial shortening rate( FS) % between groups before and after treatment were observed. Results: The tail artery blood pressure,ET-1,AngⅡ in blank control group after treatment were significant rise,ALDO,NO significantly reduced,EF%,FS% had no obvious change,while the tail artery blood pressure,ET-1,Ang Ⅱ,ALDO in other four groups were significantly reduced,NO,EF%,FS% were significantly raised,differences were statistically significant( P < 0. 05); The tail artery blood pressure,ALDO in hepatic vein on middle or high dose group were significantly lower than the captopril control group,NO,EF%,FS% were significantly higher than the captopril control group,the AngⅡ in hepatic vein on high dose group was significantly lower than the captopril control group,the differences were statistically significant( P < 0.05). Conclusions: Pinggan-Maitong slice can reduce the blood pressure in yin deficiency type hand hypertension rats,it can reverse left ventricular hypertrophy,which may related to adjusting the serum NO,ET-1,Ang Ⅱ,ALDO.
引文
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2 Werner F,Kojonazarov B,Gaβner B,et al.Endothelial actions of atrial natriuretic peptide prevent pulmonary hypertension in mice[J].Basic Res Cardiol,2016,111(2):22.
3 祁怡馨,谢雁鸣,黎元元等.基于文献分析的高血压中医证候及证候要素的研究[J].北京中医药大学学报,2014,37(11):732-735.
4 伍倩,辛效毅.高血压中医证型与相关因素的研究进展[J].新疆中医药,2015,33(1):56-58.
5 王北辰.探讨98例肾实质性高血压中医证型及肾脏病理损害等相关因素[J].中医临床研究,2015,7(13):7-9.
6 王玉民,范军铭.高血压病中医病机探讨[J].中医研究,2014,27(4):6-8.
7 杨一玲,方显明.原发性高血压中医证型的调查研究[J].中西医结合心脑血管病杂志,2014,12(2):175-177.
8 田采红,来春林,赵建强,等.心可舒片治疗高血压60例疗效观察[J].中医临床研究,2014,6(10):9-10.
9 宋磊,鲁成,王肖龙,等.高血压病中医证型与心血管危险因素的相关性研究[J].中国中医急症,2014,23(7):1224-1225,1230.
10 陈素红,刘小胖,苏敏霞,等.济脉通片对长期饮酒大鼠血压及相关活性物质的影响[J].中草药,2014,45(9):1278-1283.
11 玛依努尔·斯买拉洪.沈宝藩教授学术思想与临证经验总结及痰瘀同治法对冠心病心绞痛的疗效观察[D].北京:北京中医药大学,2012:19-20.
12 胡晓灵,孙德昱.沈宝藩教授治疗老年病高血压病的临床经验[A].中华中医药学会第十二次中医药防治老年病学术研讨会暨老年病防治科研进展学习班会议论文集[C].北京,2014:3.
13 Tang C,Wang L,Liu X,et al.Pharmacokinetic study of Gastrodia elata in rats[J].Anal Bioanal Chem,2015,407(29):8903-8910.
14 Xiao YC,Dong Q,Chi XF,et al.Simultaneous determination of gastrodin and eight nucleosides and nucleobases in Tibet culturedgastrodia elata by HPLC method[J].Zhongguo Zhong Yao Za Zhi,2014,39(19):3798-3802.
15 Seifan M,Samani AK,Berenjian A.Bioconcrete:next generation of self-healing concrete[J].Appl Microbiol Biotechnol,2016,100(6):2591-2602.
16 Katsanos K,Spiliopoulos S,Diamantopoulos A,et al.Wound healing outcomes and health-related quality-of-life changes in the ACHILLES Trial:1-year results from a prospective randomized controlled trial of infrapopliteal balloon angioplasty versus sirolimus-eluting stenting in patients with ischemic peripheral arterial disease[J].JACC Cardiovasc Interv,2016,9(3):259-267.
17 Atas E,Kismet E,Kesik V,et al.Cardiac troponin-I,brain natriuretic peptide and endothelin-1 levels in a rat model of doxorubicininduced cardiac injury[J].J Cancer Res Ther,2015,11(4):882-886.
18 Mardani S,Heidari M,Nasri H.Renin-angiotensin system blockage for reduction of plasma adiponectin level in maintenance hemodialysis patients:a randomized controlled trial[J].Iran J Kidney Dis,2016,10(2):62-67.
19 Molina MN,Ferder L,Manucha W.Emerging role of nitric oxide and heat shock proteins in Insulin Resistance[J].Curr Hypertens Rep,2016,18(1):1.
20 Song Y,He WW,Yang SM,et al.Evaluation of the ratio of plasma aldosterone to rennin concentration measured by an automated chemiluminescent immunoassay in screening for primary aldosteronis[J].Zhonghua Nei Ke Za Zhi,2016,55(1):6-10.