阴虚证、水肿证、阴虚水肿证中医动物模型的造模方法及评价
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摘要
目的总结近年来中医阴虚、水肿证动物模型的造模方法及检测方法,为中医阴虚水肿证动物模型的建立提供思路与借鉴。方法从中医病因病机、西医病因病理角度探讨及评价阴虚、水肿证的动物模型。结果中医病因病机为指导的动物模型接近中医证候实际,但稳定性、可重复性差,西医病因病理为指导的动物模型稳定性高、可重复性强,但与中医证候实际吻合度欠佳。结论中医病因病机指导下结合西医病因病机的病证结合动物模型是相对理想的中医阴虚水肿证动物模型。
Objective To summarize the modeling methods and detectio n methods of animal models of " yin deficiency" and "edema" in recent years, providing ideas and reference for the establishment of animal model of TCM " yin deficiency edema".Methods The animal models of " yin deficiency" and " edema" were discussed and evaluated from the etiology and pathogenesis of traditional Chinese medicine and the etiology and pathology of Western medicine. Results The animal model guided by the etiology and pathogenesis of traditional Chinese medicine is close to the practice of TCM syndrome, but the stability and repeatability are poor. The animal model with the guidance of the etiology and pathology of Western medicine is high and repeatable, but it is not in good agreement with the TCM syndrome. Conclusion Under the guidance of the etiology and pathogenesis of TCM, the animal model combining disease and syndrome with the etiology and pathogenesis of Western medicine is an ideal animal model of " yin deficiency and edema" in TCM.
Objective To summarize the modeling methods and detectio n methods of animal models of " yin deficiency" and "edema" in recent years, providing ideas and reference for the establishment of animal model of TCM " yin deficiency edema".Methods The animal models of " yin deficiency" and " edema" were discussed and evaluated from the etiology and pathogenesis of traditional Chinese medicine and the etiology and pathology of Western medicine. Results The animal model guided by the etiology and pathogenesis of traditional Chinese medicine is close to the practice of TCM syndrome, but the stability and repeatability are poor. The animal model with the guidance of the etiology and pathology of Western medicine is high and repeatable, but it is not in good agreement with the TCM syndrome. Conclusion Under the guidance of the etiology and pathogenesis of TCM, the animal model combining disease and syndrome with the etiology and pathogenesis of Western medicine is an ideal animal model of " yin deficiency and edema" in TCM.
引文
[1]乔天阳,王阿美,刘文兰.中医虚证动物模型的建立和评价[J].浙江中医药大学学报,2017,41(11):923-927,940.
[2]董海军,吴雷涛,杨倩倩,等.中医阴虚证动物模型制备方法概述[J].山东中医杂志,2015,34(1):74-76.
[3]吴柳花,吕圭源,李波.黄精对长期超负荷游泳致阴虚内热模型大鼠的作用研究[J].中国中药杂志,2014,39(10):1886-1891.
[4]汪泳涛,张伟荣,宋红普,等.长期超负荷游泳运动建立阴虚内热证动物模型的研究[J].中医药学刊,2003,21(7):1115-1142.
[5]俸道荣,韦斌,黄正团.高脂血症大鼠肝肾阴虚证动物模型实验研究[J].广西中医学院学报,2010,13(3):4-5,16.
[6]韩冰冰,王世军.基因芯片技术研究虚热证大鼠阴虚内热的分子机制[J].四川中医,2013,31(8):37-40.
[7]冯志毅,崔瑛,丁岗,等.地黄对“阴虚热盛”家兔血清药效指纹图的影响[J].中药材,2007,30(12):1544-1547.
[8]姜丽芳,贺伟平,李少华,等.阴虚证动物模型的造模方法与思路评述[J].山东中医药大学学报,2009,33(1):15-18.
[9]凌昌全,李敏,李德增,等.阴阳虚证与糖皮质激素受体关系的临床与实验研究[J].浙江中西医结合杂志,2001,11(9):532-533.
[10]易杰,李德新,夏永良.脾阴虚大鼠脾、肝组织蛋白激酶C活性变化的实验研究[J].中医药学刊,2002,20(3):328-329.
[11]李慧芬,张学兰,赵资堂.酒蒸女贞子对肾阴虚模型大鼠睾丸组织氧化应激损伤的保护作用[J].中成药,2014,36(10):2144-2147.
[12]毛连根,刘昌铭,王冲,等.阴虚“上火”本质特征探究[J].基础医学与临床,2017,37(10):1452-1453.
[13]戴冰,张嘉妮,杨梦琳,等.氢化可的松致肾虚证小鼠模型的建立及相关指标的评价[J].中国实验动物学报,2017,25(1):70-73.
[14]邬林祥,常绍琴,刘云乐,等.蒙药参竹精片对肾阴虚小鼠的抗疲劳及耐缺氧作用研究[J].中国民族医药杂志,2017,10(10):63-65.
[15]廖圣宝,戴敏,刘光伟,等.手术法造2KIC—RHR模型(二肾一夹高血压)[J].中国中医基础医学杂志,2003,9(2):34-37.
[16]王永炎,鲁兆麟.中医内科学[M].2版.北京:人民卫生出版社,2016:565.
[17]周宁,郑晓珂,王秋红,等.桑白皮对肾源性水肿大鼠的代谢组学研究[J].中成药,2018,40(4):788-795.
[18]薛迪,杨晓旭,汪娜,等.阿霉素诱导心源性水肿动物模型的建立及相关防治药物的研究进展[J].长春中医药大学学报,2015,31(3):654-657.
[19]王洪玉,陈平平,董婉茹,等.阿霉素诱发肾性水肿病症大鼠模型的建立[J].中国比较医学杂志,2016,26(12):5-9.
[2]董海军,吴雷涛,杨倩倩,等.中医阴虚证动物模型制备方法概述[J].山东中医杂志,2015,34(1):74-76.
[3]吴柳花,吕圭源,李波.黄精对长期超负荷游泳致阴虚内热模型大鼠的作用研究[J].中国中药杂志,2014,39(10):1886-1891.
[4]汪泳涛,张伟荣,宋红普,等.长期超负荷游泳运动建立阴虚内热证动物模型的研究[J].中医药学刊,2003,21(7):1115-1142.
[5]俸道荣,韦斌,黄正团.高脂血症大鼠肝肾阴虚证动物模型实验研究[J].广西中医学院学报,2010,13(3):4-5,16.
[6]韩冰冰,王世军.基因芯片技术研究虚热证大鼠阴虚内热的分子机制[J].四川中医,2013,31(8):37-40.
[7]冯志毅,崔瑛,丁岗,等.地黄对“阴虚热盛”家兔血清药效指纹图的影响[J].中药材,2007,30(12):1544-1547.
[8]姜丽芳,贺伟平,李少华,等.阴虚证动物模型的造模方法与思路评述[J].山东中医药大学学报,2009,33(1):15-18.
[9]凌昌全,李敏,李德增,等.阴阳虚证与糖皮质激素受体关系的临床与实验研究[J].浙江中西医结合杂志,2001,11(9):532-533.
[10]易杰,李德新,夏永良.脾阴虚大鼠脾、肝组织蛋白激酶C活性变化的实验研究[J].中医药学刊,2002,20(3):328-329.
[11]李慧芬,张学兰,赵资堂.酒蒸女贞子对肾阴虚模型大鼠睾丸组织氧化应激损伤的保护作用[J].中成药,2014,36(10):2144-2147.
[12]毛连根,刘昌铭,王冲,等.阴虚“上火”本质特征探究[J].基础医学与临床,2017,37(10):1452-1453.
[13]戴冰,张嘉妮,杨梦琳,等.氢化可的松致肾虚证小鼠模型的建立及相关指标的评价[J].中国实验动物学报,2017,25(1):70-73.
[14]邬林祥,常绍琴,刘云乐,等.蒙药参竹精片对肾阴虚小鼠的抗疲劳及耐缺氧作用研究[J].中国民族医药杂志,2017,10(10):63-65.
[15]廖圣宝,戴敏,刘光伟,等.手术法造2KIC—RHR模型(二肾一夹高血压)[J].中国中医基础医学杂志,2003,9(2):34-37.
[16]王永炎,鲁兆麟.中医内科学[M].2版.北京:人民卫生出版社,2016:565.
[17]周宁,郑晓珂,王秋红,等.桑白皮对肾源性水肿大鼠的代谢组学研究[J].中成药,2018,40(4):788-795.
[18]薛迪,杨晓旭,汪娜,等.阿霉素诱导心源性水肿动物模型的建立及相关防治药物的研究进展[J].长春中医药大学学报,2015,31(3):654-657.
[19]王洪玉,陈平平,董婉茹,等.阿霉素诱发肾性水肿病症大鼠模型的建立[J].中国比较医学杂志,2016,26(12):5-9.