丝胶对2型糖尿病大鼠视网膜NF-κB表达的影响
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摘要
目的:探讨丝胶对2型糖尿病大鼠视网膜核因子-κB(NF-κB)表达的影响。方法:48只健康雄性SD大鼠随机分为4组:正常对照组、糖尿病模型组、丝胶治疗组和导升明组,每组12只。除正常对照组外,其余3组大鼠均采用高糖高脂饮食诱导联合小剂量链脲佐菌素(25mg/kg/d,3d)腹腔注射建立2型糖尿病大鼠模型。待模型建成后,丝胶治疗组大鼠给予丝胶(2.4g/kg/d,35d)灌胃治疗,导升明组大鼠给予导升明(200mg/kg/d,35d)灌胃。Western blot法检测视网膜NF-κB蛋白的表达,RT-PCR法检测视网膜NF-κB mRNA的表达。结果:与正常对照组比较,糖尿病模型组大鼠NF-κB蛋白和mRNA的表达明显升高(P<0.05);与糖尿病模型组比较,丝胶治疗组和导升明组大鼠NF-κB蛋白和mRNA的表达显著降低(P>0.05)。结论:丝胶可通过下调NF-κB表达,发挥对糖尿病视网膜损伤的保护作用。
Objective:To investigate the effects of sericin on expression of NF-κB in retina of type 2 diabetic rats.Methods:48 male SD rats were randomly divided into 4 groups(n=12):nomal control group,diabetes model group,sericin treatment group and doxium treatment group.Except rats in nomal control group,the type 2 diabetes model rats were made by high sugar and high fat diet combined with small dose of streptozotocin(25mg/kg/d,3d) by continuous intraperitoneal injection in the other 3 groups.After the diabetes animal model was successfully established,the rats in sericin treatment group were lavaged with sericin for 35d(2.4g/kg/d),the rats in doxium treatment group were lavaged with doxium for 35d(200mg/kg/d).Western blot and RT-PCR were used to detect the expression of NF-κB protein and m RNA in retina respectively.Results:Compared with nomal control group,the NF-κB expression in retina of rats in diabetes model group increased obviously(P<0.05).Compared with diabetes model group,the NF-κB expression in retina of rats in sericin treatment group and doxium treatment group decreased obviously(P>0.05).Conclusions:Sericin has protective effects on diabetic retinophathy by downregulating NF-κB expression in retina.
Objective:To investigate the effects of sericin on expression of NF-κB in retina of type 2 diabetic rats.Methods:48 male SD rats were randomly divided into 4 groups(n=12):nomal control group,diabetes model group,sericin treatment group and doxium treatment group.Except rats in nomal control group,the type 2 diabetes model rats were made by high sugar and high fat diet combined with small dose of streptozotocin(25mg/kg/d,3d) by continuous intraperitoneal injection in the other 3 groups.After the diabetes animal model was successfully established,the rats in sericin treatment group were lavaged with sericin for 35d(2.4g/kg/d),the rats in doxium treatment group were lavaged with doxium for 35d(200mg/kg/d).Western blot and RT-PCR were used to detect the expression of NF-κB protein and m RNA in retina respectively.Results:Compared with nomal control group,the NF-κB expression in retina of rats in diabetes model group increased obviously(P<0.05).Compared with diabetes model group,the NF-κB expression in retina of rats in sericin treatment group and doxium treatment group decreased obviously(P>0.05).Conclusions:Sericin has protective effects on diabetic retinophathy by downregulating NF-κB expression in retina.
引文
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[9]Aramwit P,Towiwat P,Srichana T.Anti-inflammatory potential of silk sericin[J].Nat Prod Commum,2013,8(4):501-504.
[10]Dash R,Acharya C,Bindu PC,et a1.Antioxidant potential of silk protein sericin against hydrogen peroxide-induced oxidative fibroblasts[J].BMB Rep,2008,41(3):236-241.
[11]Preda RC,Leisk G,Omenetto F,et al.Bioengineered silk proteins to control cell and tissue functions[J].Methods Mol Biol,2013,996:19-41.
[2]Dhoot DS,Avery RL.Vascular Endothelial Growth Factor Inhibitors for Diabetic Retinopathy[J].Curr Diab Rep,2016,16(12):122.
[3]Semeraro F,Cancarini A,dell’Omo R,et al.Diabetic Retinopathy:Vascular and Inflammatory Disease[J].J Diabetes Res,2015,2015:582060.
[4]Kern TS.Contributions of inflammatory processes to the development of the early stages of diabetic retinopathy[J].Exp Diabetes Res,2007,2007:95103.
[5]Sarkar FH,Li Y,Wang Z,et al.NF-kappaB signaling pathway and its therapeutic implications in human diseases[J].Int Rev Immunol,2008,27(5):293-319.
[6]付秀美,马宝君,赵立军,等.丝胶对2型糖尿病大鼠血糖的影响[J].2008,4(4):351-353.
[7]Hoesel B,Schmid JA.The complexity of NF-κB signaling in inflammation and cancer[J].Mol Cancer,2013,12:86.
[8]Li SY,Sun WG,Jia YH,et a1.Calcium signal-initiated early activation of NF-kappaB in neurons is a neuroprotective eventin response to kainic acid-induced excitotoxicity[J].Biochemistry(Mosc),2010,75(1):101-109.
[9]Aramwit P,Towiwat P,Srichana T.Anti-inflammatory potential of silk sericin[J].Nat Prod Commum,2013,8(4):501-504.
[10]Dash R,Acharya C,Bindu PC,et a1.Antioxidant potential of silk protein sericin against hydrogen peroxide-induced oxidative fibroblasts[J].BMB Rep,2008,41(3):236-241.
[11]Preda RC,Leisk G,Omenetto F,et al.Bioengineered silk proteins to control cell and tissue functions[J].Methods Mol Biol,2013,996:19-41.