啤酒酵母细胞壁环境压力应答机制研究进展
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摘要
酵母细胞壁在细胞形态学的建立和维持中起重要作用,有助于细胞抵御环境变化。细胞壁主要由β-葡聚糖、甘露糖蛋白和几丁质组成,其组成和结构会由于压力胁迫发生动态重构。同时为了适应环境压力变化,啤酒酵母细胞壁在长期驯化过程中表现出相关压力应答机制。文中介绍了啤酒酵母细胞壁组成与结构,并综述了细胞壁重构与信号通路调控的分子机制。
Yeast cell wall plays an important role in the establishment and maintenance of cell morphology upon the cell wall stress.The cell wall of yeast consists of β-glucans,mannoproteins and chitin.The composition and structure remodel due to cell wall stress.Brewer's yeast cell wall exhibits stress response during long-term acclimation in order to adapt to environmental changes.This paper reviews the composition and structure of yeast cell wall and the molecular mechanisms of cell wall remodeling and signal pathway regulation.
Yeast cell wall plays an important role in the establishment and maintenance of cell morphology upon the cell wall stress.The cell wall of yeast consists of β-glucans,mannoproteins and chitin.The composition and structure remodel due to cell wall stress.Brewer's yeast cell wall exhibits stress response during long-term acclimation in order to adapt to environmental changes.This paper reviews the composition and structure of yeast cell wall and the molecular mechanisms of cell wall remodeling and signal pathway regulation.
引文
[1]Klis FM,Mol P,Hellingwerf K,et al.Dynamics of cell wall structure in Saccharomyces cerevisiae.FEMSMicrobiol Rev,2002,26(3):239-256.
[2]Wang JJ,Xu WN,Li XE,et al.Absence of Fks1p in lager brewing yeast results in aberrant cell wall composition and improved beer flavor stability.World JMicrobiol Biotechnol,2014,30(6):1901-1908.
[3]Alexandre H,Guilloux-Benatier M.Yeast autolysis in sparkling wine-a review.Aust J Grape Wine Res,2006,12(2):119-127.
[4]Xu WN,Wang JJ,Chen X,et al.Changes in autolysis solution of brewer’s yeasts and the evaluation of autolysis.J Food Sci Biotechnol,2013,32(6):574-580(in Chinese).许维娜,王金晶,陈希,等.啤酒酵母自溶液中的物质变化及酵母自溶评价指标探索.食品与生物技术学报,2013,32(6):574-580.
[5]Wang JJ,Yang JJ,Ding HJ,et al.Research progress in yeast global stress response during beer brewing.Food Ferment Ind,2017,43(1):266-270,275(in Chinese).王金晶,杨静静,丁华建,等.啤酒发酵过程中酵母环境压力应答机制研究进展.食品与发酵工业,2017,43(1):266-270,275.
[6]Lesage G,Bussey H.Cell wall assembly in Saccharomyces cerevisiae.Microbiol Mol Biol Rev,2006,70(2):317-343.
[7]Wang JJ,Li MQ,Zheng FY,et al.Cell wall polysaccharides:before and after autolysis of brewer’s yeast.World J Microbiol Biotechnol,2018,34(9):137.
[8]Sanz AB,García R,Rodriguez-Pe?a JM,et al.The CWIpathway:regulation of the transcriptional adaptive response to cell wall stress in yeast.J Fungi,2018,4(1):E1.
[9]Orlean P.Architecture and biosynthesis of the Saccharomyces cerevisiae cell wall.Genetics,2012,192(3):775-818.
[10]Lipke PN,Ovalle R.Cell wall architecture in yeast:new structure and new challenges.J Bacteriol,1998,180(15):3735-3740.
[11]Klis FM,Boorsma A,De Groot PWJ.Cell wall construction in Saccharomyces cerevisiae.Yeast,2006,23(3):185-202.
[12]Gibson BR,Lawrence SJ,Leclaire JPR,et al.Yeast responses to stresses associated with industrial brewery handling.FEMS Microbiol Rev,2007,31(5):535-569.
[13]Arroyo J,Farka?V,Sanz AB,et al.Strengthening the fungal cell wall through chitin-glucan cross-links:effects on morphogenesis and cell integrity.Cell Microbiol,2016,18(9):1239-1250.
[14]Ene IV,Walker LA,Schiavone M,et al.Cell wall remodeling enzymes modulate fungal cell wall elasticity and osmotic stress resistance.mBio,2015,6(4):e00986-15.
[15]Li J,Wang JJ,Li Q.Overexpression of FKS1 to improve yeast autolysis-stress.Chin J Biotech,2015,31(9):1344-1354(in Chinese).李佳,王金晶,李崎.18S rDNA介导的FKS1基因过表达对酵母自溶性能的影响.生物工程学报,2015,31(9):1344-1354.
[16]Bzducha-Wróbel A,B?a?ejak S,Kieliszek M,et al.Modification of the cell wall structure of Saccharomyces cerevisiae strains during cultivation on waste potato juice water and glycerol towards biosynthesis of functional polysaccharides.J Biotechnol,2018,281:1-10.
[17]Li XE,Wang JJ,Phornsanthia S,et al.Strengthening of cell wall structure enhances stress resistance and fermentation performance in lager yeast.J Am Soc Brew Chem,2014,72(2):88-94.
[18]Bzducha-Wróbel A,Kieliszek M,B?a?ejak S.Chemical composition of the cell wall of probiotic and brewer’s yeast in response to cultivation medium with glycerol as a carbon source.Eur Food Res Technol,2013,237(4):489-499.
[19]Sestak S,Hagen I,Tanner W,et al.Scw10p,a cell-wall glucanase/transglucosidase important for cell-wall stability in Saccharomyces cerevisiae.Microbiology,2004,150(10):3197-3208.
[20]Tudela R,Gallardo-Chacón JJ,Rius N,et al.Ultrastructural changes of sparkling wine lees during long-term aging in real enological conditions.FEMSYeast Res,2012,12(4):466-476.
[21]Lawrence SJ,Gibson BR,Smart KA.Expression of the cell wall mannoprotein genes CWP and DAN during industrial-scale lager fermentations.J Am Soc Brew Chem,2009,67(1):58-62.
[22]Bastos R,Coelho E,Coimbra MA.Modifications of Saccharomyces pastorianus cell wall polysaccharides with brewing process.Carbohydr Polym,2015,124:322-330.
[23]Bzducha-Wróbel A,B?a?ejakS,Kawarska A,et al.Evaluation of the efficiency of different disruption methods on yeast cell wall preparation forβ-glucan isolation.Molecules,2014,19(12):20941-20961.
[24]Tabera L,Mu?oz R,Gonzalez R.Deletion of BCY1 from the Saccharomyces cerevisiae genome is semidominant and induces autolytic phenotypes suitable for improvement of sparkling wines.Appl Environ Microbiol,2006,72(4):2351-2358.
[25]Jiang B,Ram AFJ,Sheraton J,et al.Regulation of cell wallβ-glucan assembly:PTC1 Negatively affects PBS2Action in a pathway that includes modulation of EXG1transcription.Mol Gen Genet,1995,248(3):260-269.
[26]Rodríguez-pe?a JM,Cid VJ,Arroyo J,et al.A novel family of cell wall-related proteins regulated differently during the yeast life cycle.Mol Cell Biol,2000,20(9):3245-3255.
[27]Cabib E.Two novel techniques for determination of polysaccharide cross-links show that Crh1p and Crh2p attach chitin to bothβ(1-6)-andβ(1-3)glucan in the Saccharomyces cerevisiae cell wall.Eukaryot Cell,2009,8(11):1626-1636.
[28]Wang JJ,Mao JC,Yang G,et al.The FKS family genes cause changes in cell wall morphology resulted in regulation of anti-autolytic ability in Saccharomyces cerevisiae.Bioresour Technol,2018,249:49-56.
[29]Smits GJ,Kapteyn JC,van den Ende H,et al.Cell wall dynamics in yeast.Curr Opin Microbiol,1999,2(4):348-352.
[30]Kono K,Ikui AE.A new cell cycle checkpoint that senses plasma membrane/cell wall damage in budding yeast.BioEssays,2017,39(4):1600210.
[31]Jendretzki A,Wittland J,Wilk S,et al.How do I begin?Sensing extracellular stress to maintain yeast cell wall integrity.Eur J Cell Biol,2011,90(9):740-744.
[32]Yang G.The function of FKS family genes on the yeast cells morphology and anti-autolytic ability[D].Wuxi:Jiangnan University,2017(in Chinese).杨歌.FKS家族基因对酵母细胞形态及抗自溶性能的作用[D].无锡:江南大学,2017.
[33]Xu WN,Wang JJ,Li Q.Microarray studies on lager brewer’s yeasts reveal cell status in the process of autolysis.FEMS Yeast Res,2014,14(5):714-728.
[34]Arroyo J,Bermejo C,García R,et al.Genomics in the detection of damage in microbial systems:cell wall stress in yeast.Clin Microbiol Infect,2009,15(S1):44-46.
[35]Xu WN,Wang JJ,Li Q.Comparative proteome and transcriptome analysis of lager brewer’s yeast in the autolysis process.FEMS Yeast Res,2014,14(8):1273-1285.
[36]Bühligen F,Rüdinger P,Fetzer I,et al.Sustainability of industrial yeast serial repitching practice studied by gene expression and correlation analysis.J Biotechnol,2013,168(4):718-728.
[37]Dunayevich P,Baltanás R,Clemente JA,et al.Heat-stress triggers MAPK crosstalk to turn on the hyperosmotic response pathway.Sci Rep,2018,8:15168.
[38]Winkler A,Arkind C,Mattison CP,et al.Heat stress activates the yeast high-osmolarity glycerol mitogen-activated protein kinase pathway,and protein tyrosine phosphatases are essential under heat stress.Eukar Cell,2002,1(2):163-173.
[39]Levin DE.Cell wall integrity signaling in Saccharomyces cerevisiae.Microbiol Mol Biol Rev,2005,69(2):262-291.
[40]Haghnazari E,Heyer WD.The Hog1 MAP kinase pathway and the Mec1 DNA damage checkpoint pathway independently control the cellular responses to hydrogen peroxide.DNA Repair,2004,3(7):769-776.
[41]Bermejo C,Rodríguez E,García R,et al.The sequential activation of the yeast HOG and SLT2 pathways is required for cell survival to cell wall stress.Mol Biol Cell,2008,19(3):1113-1124.
[42]Rodriguez-Pe?a JM,García R,Nombela C,et al.The high-osmolarity glycerol(HOG)and cell wall integrity(CWI)signalling pathways interplay:a yeast dialogue between MAPK routes.Yeast,2010,27(8):495-502.
[2]Wang JJ,Xu WN,Li XE,et al.Absence of Fks1p in lager brewing yeast results in aberrant cell wall composition and improved beer flavor stability.World JMicrobiol Biotechnol,2014,30(6):1901-1908.
[3]Alexandre H,Guilloux-Benatier M.Yeast autolysis in sparkling wine-a review.Aust J Grape Wine Res,2006,12(2):119-127.
[4]Xu WN,Wang JJ,Chen X,et al.Changes in autolysis solution of brewer’s yeasts and the evaluation of autolysis.J Food Sci Biotechnol,2013,32(6):574-580(in Chinese).许维娜,王金晶,陈希,等.啤酒酵母自溶液中的物质变化及酵母自溶评价指标探索.食品与生物技术学报,2013,32(6):574-580.
[5]Wang JJ,Yang JJ,Ding HJ,et al.Research progress in yeast global stress response during beer brewing.Food Ferment Ind,2017,43(1):266-270,275(in Chinese).王金晶,杨静静,丁华建,等.啤酒发酵过程中酵母环境压力应答机制研究进展.食品与发酵工业,2017,43(1):266-270,275.
[6]Lesage G,Bussey H.Cell wall assembly in Saccharomyces cerevisiae.Microbiol Mol Biol Rev,2006,70(2):317-343.
[7]Wang JJ,Li MQ,Zheng FY,et al.Cell wall polysaccharides:before and after autolysis of brewer’s yeast.World J Microbiol Biotechnol,2018,34(9):137.
[8]Sanz AB,García R,Rodriguez-Pe?a JM,et al.The CWIpathway:regulation of the transcriptional adaptive response to cell wall stress in yeast.J Fungi,2018,4(1):E1.
[9]Orlean P.Architecture and biosynthesis of the Saccharomyces cerevisiae cell wall.Genetics,2012,192(3):775-818.
[10]Lipke PN,Ovalle R.Cell wall architecture in yeast:new structure and new challenges.J Bacteriol,1998,180(15):3735-3740.
[11]Klis FM,Boorsma A,De Groot PWJ.Cell wall construction in Saccharomyces cerevisiae.Yeast,2006,23(3):185-202.
[12]Gibson BR,Lawrence SJ,Leclaire JPR,et al.Yeast responses to stresses associated with industrial brewery handling.FEMS Microbiol Rev,2007,31(5):535-569.
[13]Arroyo J,Farka?V,Sanz AB,et al.Strengthening the fungal cell wall through chitin-glucan cross-links:effects on morphogenesis and cell integrity.Cell Microbiol,2016,18(9):1239-1250.
[14]Ene IV,Walker LA,Schiavone M,et al.Cell wall remodeling enzymes modulate fungal cell wall elasticity and osmotic stress resistance.mBio,2015,6(4):e00986-15.
[15]Li J,Wang JJ,Li Q.Overexpression of FKS1 to improve yeast autolysis-stress.Chin J Biotech,2015,31(9):1344-1354(in Chinese).李佳,王金晶,李崎.18S rDNA介导的FKS1基因过表达对酵母自溶性能的影响.生物工程学报,2015,31(9):1344-1354.
[16]Bzducha-Wróbel A,B?a?ejak S,Kieliszek M,et al.Modification of the cell wall structure of Saccharomyces cerevisiae strains during cultivation on waste potato juice water and glycerol towards biosynthesis of functional polysaccharides.J Biotechnol,2018,281:1-10.
[17]Li XE,Wang JJ,Phornsanthia S,et al.Strengthening of cell wall structure enhances stress resistance and fermentation performance in lager yeast.J Am Soc Brew Chem,2014,72(2):88-94.
[18]Bzducha-Wróbel A,Kieliszek M,B?a?ejak S.Chemical composition of the cell wall of probiotic and brewer’s yeast in response to cultivation medium with glycerol as a carbon source.Eur Food Res Technol,2013,237(4):489-499.
[19]Sestak S,Hagen I,Tanner W,et al.Scw10p,a cell-wall glucanase/transglucosidase important for cell-wall stability in Saccharomyces cerevisiae.Microbiology,2004,150(10):3197-3208.
[20]Tudela R,Gallardo-Chacón JJ,Rius N,et al.Ultrastructural changes of sparkling wine lees during long-term aging in real enological conditions.FEMSYeast Res,2012,12(4):466-476.
[21]Lawrence SJ,Gibson BR,Smart KA.Expression of the cell wall mannoprotein genes CWP and DAN during industrial-scale lager fermentations.J Am Soc Brew Chem,2009,67(1):58-62.
[22]Bastos R,Coelho E,Coimbra MA.Modifications of Saccharomyces pastorianus cell wall polysaccharides with brewing process.Carbohydr Polym,2015,124:322-330.
[23]Bzducha-Wróbel A,B?a?ejakS,Kawarska A,et al.Evaluation of the efficiency of different disruption methods on yeast cell wall preparation forβ-glucan isolation.Molecules,2014,19(12):20941-20961.
[24]Tabera L,Mu?oz R,Gonzalez R.Deletion of BCY1 from the Saccharomyces cerevisiae genome is semidominant and induces autolytic phenotypes suitable for improvement of sparkling wines.Appl Environ Microbiol,2006,72(4):2351-2358.
[25]Jiang B,Ram AFJ,Sheraton J,et al.Regulation of cell wallβ-glucan assembly:PTC1 Negatively affects PBS2Action in a pathway that includes modulation of EXG1transcription.Mol Gen Genet,1995,248(3):260-269.
[26]Rodríguez-pe?a JM,Cid VJ,Arroyo J,et al.A novel family of cell wall-related proteins regulated differently during the yeast life cycle.Mol Cell Biol,2000,20(9):3245-3255.
[27]Cabib E.Two novel techniques for determination of polysaccharide cross-links show that Crh1p and Crh2p attach chitin to bothβ(1-6)-andβ(1-3)glucan in the Saccharomyces cerevisiae cell wall.Eukaryot Cell,2009,8(11):1626-1636.
[28]Wang JJ,Mao JC,Yang G,et al.The FKS family genes cause changes in cell wall morphology resulted in regulation of anti-autolytic ability in Saccharomyces cerevisiae.Bioresour Technol,2018,249:49-56.
[29]Smits GJ,Kapteyn JC,van den Ende H,et al.Cell wall dynamics in yeast.Curr Opin Microbiol,1999,2(4):348-352.
[30]Kono K,Ikui AE.A new cell cycle checkpoint that senses plasma membrane/cell wall damage in budding yeast.BioEssays,2017,39(4):1600210.
[31]Jendretzki A,Wittland J,Wilk S,et al.How do I begin?Sensing extracellular stress to maintain yeast cell wall integrity.Eur J Cell Biol,2011,90(9):740-744.
[32]Yang G.The function of FKS family genes on the yeast cells morphology and anti-autolytic ability[D].Wuxi:Jiangnan University,2017(in Chinese).杨歌.FKS家族基因对酵母细胞形态及抗自溶性能的作用[D].无锡:江南大学,2017.
[33]Xu WN,Wang JJ,Li Q.Microarray studies on lager brewer’s yeasts reveal cell status in the process of autolysis.FEMS Yeast Res,2014,14(5):714-728.
[34]Arroyo J,Bermejo C,García R,et al.Genomics in the detection of damage in microbial systems:cell wall stress in yeast.Clin Microbiol Infect,2009,15(S1):44-46.
[35]Xu WN,Wang JJ,Li Q.Comparative proteome and transcriptome analysis of lager brewer’s yeast in the autolysis process.FEMS Yeast Res,2014,14(8):1273-1285.
[36]Bühligen F,Rüdinger P,Fetzer I,et al.Sustainability of industrial yeast serial repitching practice studied by gene expression and correlation analysis.J Biotechnol,2013,168(4):718-728.
[37]Dunayevich P,Baltanás R,Clemente JA,et al.Heat-stress triggers MAPK crosstalk to turn on the hyperosmotic response pathway.Sci Rep,2018,8:15168.
[38]Winkler A,Arkind C,Mattison CP,et al.Heat stress activates the yeast high-osmolarity glycerol mitogen-activated protein kinase pathway,and protein tyrosine phosphatases are essential under heat stress.Eukar Cell,2002,1(2):163-173.
[39]Levin DE.Cell wall integrity signaling in Saccharomyces cerevisiae.Microbiol Mol Biol Rev,2005,69(2):262-291.
[40]Haghnazari E,Heyer WD.The Hog1 MAP kinase pathway and the Mec1 DNA damage checkpoint pathway independently control the cellular responses to hydrogen peroxide.DNA Repair,2004,3(7):769-776.
[41]Bermejo C,Rodríguez E,García R,et al.The sequential activation of the yeast HOG and SLT2 pathways is required for cell survival to cell wall stress.Mol Biol Cell,2008,19(3):1113-1124.
[42]Rodriguez-Pe?a JM,García R,Nombela C,et al.The high-osmolarity glycerol(HOG)and cell wall integrity(CWI)signalling pathways interplay:a yeast dialogue between MAPK routes.Yeast,2010,27(8):495-502.