大豆花粉活力测定方法的比较研究
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摘要
为研究不同方法测定大豆花粉活力的问题,选取‘绥农4’、‘吉林47’和‘CK-P’为研究对象,通过蔗糖、琼脂、硼酸、温度单因子正交试验筛选出的培养基进行离体萌发,比较I2-KI染色法、TTC染色法和离体萌发法3种花粉活力测定方法的不同。结果表明:I2-KI染色法容易操作,因这种方法较离体萌发法简单,测定结果比较准确,成本低,所需时间短,常用来观测大豆花粉活力。TTC法染色效果与I2-KI染色法接近,没有差异,也可作为检测大豆花粉活力的方法。离体萌发法测定大豆花粉活力的方法和很多因素有关,与I2-KI染色法和TTC染色法有显著差异,且其培养基的配制繁琐,所以用I2-KI染色法和TTC染色法来测定大豆的花粉活力。
To study different methods for determining soybean pollen vitality,‘Suinong4’‘,Jilin47’and‘CK-P’were used as the materials,a single factor orthogonal test was conducted based on sucrose,agar,H_3BO_3 and temperature,to screen culture medium for pollen germination,and the difference of pollen viability under I_2-KI,TTC staining and in vitro germination were compared.The results showed that the I_2-KI staining method was easy to operate,and was simpler than the in vitro germination method,its determination result was accurate,the cost was low and the time was short,so it was suitable for testing pollen vitality.TTC staining was similar to I_2-KI staining,and could also be used as a method to detect soybean pollen vitality.The method of in vitro germination of pollen viability was related to many factors,and was significantly different from I_2-KI and TTC staining,and its preparation was complex,so I_2-KI and TTC staining method could be used to determine soybean pollen viability.soybean;pollen vitality;culture;staining method
To study different methods for determining soybean pollen vitality,‘Suinong4’‘,Jilin47’and‘CK-P’were used as the materials,a single factor orthogonal test was conducted based on sucrose,agar,H_3BO_3 and temperature,to screen culture medium for pollen germination,and the difference of pollen viability under I_2-KI,TTC staining and in vitro germination were compared.The results showed that the I_2-KI staining method was easy to operate,and was simpler than the in vitro germination method,its determination result was accurate,the cost was low and the time was short,so it was suitable for testing pollen vitality.TTC staining was similar to I_2-KI staining,and could also be used as a method to detect soybean pollen vitality.The method of in vitro germination of pollen viability was related to many factors,and was significantly different from I_2-KI and TTC staining,and its preparation was complex,so I_2-KI and TTC staining method could be used to determine soybean pollen viability.soybean;pollen vitality;culture;staining method
引文
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[10]李继爱,姜贺飞,赵慧恩.洋水仙花粉生活力的测定花粉初探[J].中国农学通报,2016,26(17):242-245.
[11]于晓英.刮叶菊花粉生活力测定[J].湖南农业大学学报,2005(2):41-45.
[12]廖明安,沈向,司龙亭,等.园艺植物研究法[M].北京:中国农业出版社,2005:140.
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[14]赵统利,周翔,朱朋波,等.百合花粉生活力测定方法的比较研究[J].江苏农业科学,2006(6):88.
[15]王国荣.花粉萌发实验的改进报告[J].中学生物教学,2002(5):40.
[16]郭光明,张福锁,尚忠林,等.硼对百合花粉萌发过程中细胞内游离钙离子的影响[J].中国农业大学学报,2002,7(5):32-37.
[17]王超,张桂玲.甘蓝花粉萌发离体培养最佳蔗糖硼酸配方初探[J].园艺学报,2004,31(2):209.
[18]Kakegawa K,Ishii T,Matsunaga T.Effects of boron deficiency in cell suspension cultures of Populus alba L.[J].Plant Cell Rep,2005,23:573-578.
[19]年玉欣,罗凤霞,张颖,等.测定百合花粉生活力的液体培养基研究[J].园艺学报,2005,32(5):922-925.
[20]杜克兵,沈宝仙,许林,等.不同贮藏条件下杨树花粉活力变化及隔年杂交授粉应用的可行性研究[J].华中农业大学学报,2007,26(3):385-389.
[2]王志新,郭泰,赵丽梅,等.大豆杂交种异地鉴定及选育方法初探[J].中国农学通报,2013,29(36):114-118.
[3]赵鸿杰,乔龙巴图,殷爱华,等.3种山茶属植物花粉活力测定方法的比较[J].中南林业科技大学学报,2010,30(3):105-107.
[4]张超仪,耿兴敏.六种杜鹃花属植物花粉活力测定方法的比较研究[J].植物科学学报,2012,30(1):92-99.
[5]孙春丽,潘延云.拟南芥花粉活力的测定及其在花粉发育研究中的应用[J].植物学通报,2008,25(3):268-275.
[6]王艳哲,崔彦宏,张丽华,等.玉米花粉活力测定方法的比较研究[J].玉米科学,2010,18(3):173-176.
[7]韩成刚,盖树鹏.芍药花粉活力测定方法的研究[J].江苏农业科学,2012,40(5):124-126.
[8]王刚,刘辉,吕新,等.新陆早43号棉花父本花粉活力测定方法的比较[J].江苏农业科学,2013,41(2):88-90.
[9]张志良.植物生理学实验指导[M].北京:高等教育出版社,2000:240-242.
[10]李继爱,姜贺飞,赵慧恩.洋水仙花粉生活力的测定花粉初探[J].中国农学通报,2016,26(17):242-245.
[11]于晓英.刮叶菊花粉生活力测定[J].湖南农业大学学报,2005(2):41-45.
[12]廖明安,沈向,司龙亭,等.园艺植物研究法[M].北京:中国农业出版社,2005:140.
[13]胡晋.花粉的保存和生活力的测定[J].种子,1990(6):33-35.
[14]赵统利,周翔,朱朋波,等.百合花粉生活力测定方法的比较研究[J].江苏农业科学,2006(6):88.
[15]王国荣.花粉萌发实验的改进报告[J].中学生物教学,2002(5):40.
[16]郭光明,张福锁,尚忠林,等.硼对百合花粉萌发过程中细胞内游离钙离子的影响[J].中国农业大学学报,2002,7(5):32-37.
[17]王超,张桂玲.甘蓝花粉萌发离体培养最佳蔗糖硼酸配方初探[J].园艺学报,2004,31(2):209.
[18]Kakegawa K,Ishii T,Matsunaga T.Effects of boron deficiency in cell suspension cultures of Populus alba L.[J].Plant Cell Rep,2005,23:573-578.
[19]年玉欣,罗凤霞,张颖,等.测定百合花粉生活力的液体培养基研究[J].园艺学报,2005,32(5):922-925.
[20]杜克兵,沈宝仙,许林,等.不同贮藏条件下杨树花粉活力变化及隔年杂交授粉应用的可行性研究[J].华中农业大学学报,2007,26(3):385-389.