种子萌发期高粱抗旱材料的筛选与鉴定
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摘要
种子萌发期,筛选、鉴定高粱抗旱材料对挖掘种子萌发期抗旱基因以及培育抗旱性更强的高粱品种都具有重要意义。本研究采用聚乙二醇(PEG6000)模拟干旱胁迫,设置0(CK)、5%、10%、15%、17.5%、20%、22.5%和25%8个不同浓度梯度PEG6000对粒用高粱BTx623和甜高粱Rio进行干旱处理,确定PEG6000初步筛选浓度,然后利用PEG6000初步筛选浓度对全球收集的396份高粱进行初步筛选,筛选出较抗旱的材料逐渐加大选择压力,最终筛选出高抗旱性的高粱材料。基于以上试验,确定了大批材料初步筛选时PEG6000浓度为15%较为适宜。396份高粱材料经过15%PEG6000初步筛选时,相对出苗率达到80%以上的有89份,逐步加大筛选压力,最终筛选出4份抗旱能力很强的高粱材料,其中3份来自中国的粒用高粱,1份来自东亚的粒用高粱。随着处理浓度的增加,高粱抗旱性和耐盐性两者之间的相关性存在不确定性。
At the seed germin at ion stage,screening and identification the drought-resistant materials,which is great significance for exploring drought-resistant genes and cultivating the drought-resistant varieties.In this research,we set,0(CK),5%,10%,15%,17.5%,20%,22.5% and 25%,8 different concentration gradients PEG6000 for drought treatment of grain sorghum BTx623 and sweet sorghum Rio,which was aim at determine the initial screening concentration of PEG6000.Then,396 sorghum materials,from the whole world,were treated by the PEG6000 initial screening concentration to screen drought-resistant materials,and then gradually increase selection pressure to screen stronger drought-resistant materials.Based on the above test,we confirmed that 15% PEG6000 was appropriate for initial screening of a large number of materials.396 sorghum materials were treated by 15%PEG6000,89 higher drought-resistant materials were obtained,which the relative seedling-emergence rates were more than 80%.Step up the screening pressure,4 mostly stronger drought-resistant materials were obtained.3 materials of them were from China,1 material were from East Asia,which were all grain sorghum.With the increasing of treatment concentration,the correlation between drought resistance and salinity tolerance of sorghum might exist uncertainty.
At the seed germin at ion stage,screening and identification the drought-resistant materials,which is great significance for exploring drought-resistant genes and cultivating the drought-resistant varieties.In this research,we set,0(CK),5%,10%,15%,17.5%,20%,22.5% and 25%,8 different concentration gradients PEG6000 for drought treatment of grain sorghum BTx623 and sweet sorghum Rio,which was aim at determine the initial screening concentration of PEG6000.Then,396 sorghum materials,from the whole world,were treated by the PEG6000 initial screening concentration to screen drought-resistant materials,and then gradually increase selection pressure to screen stronger drought-resistant materials.Based on the above test,we confirmed that 15% PEG6000 was appropriate for initial screening of a large number of materials.396 sorghum materials were treated by 15%PEG6000,89 higher drought-resistant materials were obtained,which the relative seedling-emergence rates were more than 80%.Step up the screening pressure,4 mostly stronger drought-resistant materials were obtained.3 materials of them were from China,1 material were from East Asia,which were all grain sorghum.With the increasing of treatment concentration,the correlation between drought resistance and salinity tolerance of sorghum might exist uncertainty.
引文
Crasta O.R.,Xu W.W.,Rosenow D.T.,Mullet J.,and Nguyen H.T.,1999,Mapping of post-flowering drought resistance traits in grain sorghum:association between QTLs influencing premature senescence and maturity,Mol.Gen.Genet.,262(3):579-588
Fracasso A.,Trindade L.M.,and Amaducci S.,2016,Drought stress tolerance strategies revealed by RNA-Seq in two sorghum genotypes with contrasting WUE,BMC Plant Biol.,16(1):115-132
Hamza N.B.,Sharma N.,Tripathi A.,and Sanan-Mishra N.,2016,Micro RNA expression profiles in response to drought stress in Sorghum bicolor,Gene Exp.Patterns,20(2):88-98
Harris K.,Subudhi P.K.,Borrell A.,Jordan D.,Rosenow D.Nguyen H.,Klein P.,Klein R.,and Mullet J.,2007,Sorghum stay-green QTL individually reduce post-flowering droughtinduced leaf senescence,J.Exp.Bot.,58(2):327-338
He H.,Yan J.,Yu X.Y.,Liang Y.,Fang L.,Scheller H.V.,and Zhang A.Y.,2017,The NADPH-oxidase At Rboh I plays a positive role in drought-stress response in Arabidopsis thaliana,Biochem.Biophys.Res.Commun.,491(3):834-839
Johnson S.M.,Lim F.L.,Finkler A.,Fromm H.,Slabas A.R.,and Knight M.R.,2014,Transcriptomic analysis of Sorghum bicolor responding to combined heat and drought stress,BMCGenomics,15(1):456-474
Katiyar A.,Smita S.,Muthusamy S.K.,Chinnusamy V.,Pandey D.M.,and Bansal K.C.,2015,Identification of nove drought-responsive micro RNAs and trans-acting si RNAs from Sorghum bicolor(L.)Moench by high-throughput sequencing analysis,Front Plant Sci.,6:506-526
Kebede H.,Subudhi P.K.,Rosenow D.T.,and Nguyen H.T.2001,Quantitative trait loci influencing drought tolerance in grain sorghum(Sorghum bicolor L.Moench),Theor.Appl Genet.,103(2-3):266-276
Li S.J.,Zhu J.Q.,Ye X.Y.,Wang L.H.,Song Y.Y.,and Wang HZ.,2005,Double tolerant differences of rice under drough and salt stresses,Xinan Nongye Xuebao(Southwest China Journal of Agricultural Science),18(2):128-132(李姝晋,朱建清,叶小英,王丽华,宋永燕,王贺正,2005,干旱和盐胁迫下水稻品种的双重耐性差异,西南农业学报,18(2)128-132)
Li T.T.,Gao Y.F.,Ma X.,Chen Y.F.,Wang Y.,and Ma J.B.2016,Effects of exogenous brassinosteroid on photosynthesis of three spesies of Populus under drought,salt and copper stress,Jiyinzuxue Yu Yingyong Shengwuxue(Genomics and Applied Biology),36(1):218-226(李涛涛,高永峰,马瑄,陈永富,王阳,马金彪,2016,外源油菜素内酯对三种杨树在干旱、盐和铜胁迫下光合生理的影响,基因组学与应用生物学,36(1):218-226)
Li Z.Y.,Long R.C.,Zhang T.J.,Wang Z.,Zhang F.,Yang Q.C.Kang J.M.,and Sun Y.,2017,Molecular cloning and functional analysis of the drought tolerance gene Ms HSP70 from alfalfa(Medicago sativa L.),J.Plant Res.,130(2):387-396
Liang X.H.,Yi Z.B.,and Zhao W.J.,2005,Research advance on mapping the important resistant-genes of sorghum,Zuowu Zazhi(Crops),(3):7-9(梁小红,仪治本,赵威军,2005,高粱重要抗性性状的基因定位研究综述,作物杂志,(3):7-9)
Liu Z.,2008,Study and appraisal of salt tolerance drought resistance capacity of different alfalfa cultivar,Thesis for M.S.Jilin Agricultural University,Supervisor:Xu A.K.,pp.37-40(刘卓,2008,不同苜蓿品种性、抗旱性比较的研究,硕士学位论文,吉林农业大学,导师:徐安凯,pp.37-40)
Lv X.,Zhang F.Y.,Ping J.A.,Du Z.H.,Li H.M.,Tian Z.X.,Yang T.T.,and Qiao J.,2013,Correlation analysis of 25 main agronomic characters and drought resistance in forage sorghum restorer lines,Zhongguo Nongxue Tongbao(Chinese Agricultural Science Bulletin),29(29):6-13(吕鑫,张福耀,平俊爱,杜志宏,李慧明,田兆祥,杨婷婷,乔婧,2013,25个饲草高粱恢复系主要农艺性状及其抗旱性的相关分析,中国农学通报,29(29):6-13)
Mao H.D.,Wang H.W.,Liu S.X.,Li Z.G.,Yang X.H.,Yan J.B.,Li J.S.,Tran L.S.P.,and Qin F.,2015,A transposable element in a NAC gene is associated with drought tolerance in maize seedlings,Nat.Commun.,6:8326-8339
Menezes C.B.,Ticona-Benavente C.A.,Tardin F.D.,Cardoso M.J.,Bastos E.A.,Nogueira D.W.,Portugal A.F.,Santos C.V.,and Schaffert R.E.,2014,Selection indices to identify drought-tolerant grain sorghum cultivars,Genet.Mol.Res.,13(4):9817-9827
Paterson A.H.,Bowers J.E.,Bruggmann R.,Dubchak I.,Grimwood J.,Gundlach H.,Haberer G.,Hellsten U.,Mitros T.,Poliakov A.,Schmutz J.,Spannagl M.,Tang H.B.,Wang X.Y.,Wicker T.,Bharti A.K.,Chapman J.,Feltus F.A.,Gowik U.,Grigoriev I.V.,Lyons E.,Maher C.A.,Martis M.,Narechania A.,Otillar R.P.,Penning B.W.,Salamov A.A.,Wang Y.,Zhang L.F.,Carpita N.C.,Freeling M.,Gingle A.R.,Hash C.T.,Keller B.,Klein P.,Kresovich S.,Mc Cann M.C.,Ming R.,Peterson D.G.,Mehboob-ur-Rahman,Ware D.,Westhoff P.,Mayer K.F.X.,Messing J.,and Rokhsar D.S.,2009,The sorghum bicolor genome and the diversification of grasses,Nature,457(7229):551-556
Rama Reddy N.R.,Ragimasalawada M.,Sabbavarapu M.M.,Nadoor S.,and Patil J.V.,2014,Detection and validation of stay-green QTL in post-rainy sorghum involving widely adapted cultivar,M35-1 and a popular stay-green genotype B35,BMC Genomics,15(1):909-924
Tuinstra M.R.,Grote E.M.,Goldsbrough P.B.,and Ejeta G.,1996,Identification of quantitative trait loci associated with pre-flowering drought tolerance in sorghum,Crop Sci.,36(5):1337-1344
Wu Q.,Zhou Y.F.,Gao Y.,Zhang J.,Chen B.R.,Xu W.J.,and Huang R.D.,2016,Screening and identification for drought resistance during germination in sorghum cultivars,Zuowu Xuebao(Acta Agronomica Sinica),42(8):1233-1246(吴奇,周宇飞,高悦,张姣,陈冰嬬,许文娟,黄瑞冬,2016,不同高粱品种萌发期抗旱性筛选与鉴定,作物学报,42(8):1233-1246)
Xu W.W.,Subudhi P.K.,Crasta O.R.,Rosenow D.T.,Mullet J.E.,and Nguyen H.T.,2000,Molecular mapping of QTLs conferring stay-green in grain sorghum(Sorghum bicolor L.Moench),Genome,43(3):461-469
Yang F.,Wei X.C.,Zhang S.C.,and Wang B.S.,2015,Comparison on salt and drought resistances of different varieties of Sorghum bicolor at germination stage,Zhiwu Shengli Xue bao(Plant Physiology Journal),51(10):1604-1610(杨帆,魏晓岑,张士超,王宝山,2015,不同甜高粱品种萌发期抗盐和抗旱性比较,植物生理学报,51(10):1604-1610)
Yi Z.B.,Liang X.H.,Sun Y.,Li B.L.,Wang J.X.,Zhao W.J.,and Lv H.Q.,2003,Research advance on mapping quantitative trait loci of drought resistance in sorghum,Zaliang Zuowu(Rain Fed Crops),23(5):262-268(仪治本,梁小红,孙毅李炳林,王景雪,赵威军,吕慧卿,2003,高粱抗旱性数量性状位点基因定位研究进展,杂粮作物,23(5):262-268)
Zhang C.,Li C.,Liu J.,Lv Y.,Yu C.,Li H.,Zhao T.,and Liu B.,2017a,The Os ABF1 transcription factor improves drought tolerance by activating the transcription of COR413-TM1 in rice,J.Exp.Bot.,68(16):4695-4707
Zhang N.,Yin Y.J.,Liu X.Y.,Tong S.M.,Xing J.W.,Zhang Y.,Pudake R.N.,Izquierdo E.M.,Peng H.,Xin M.M.,Hu Z.R.,Ni Z.F.,Sun Q.X.,and Yao Y.Y.,2017b,The E3 ligase Ta S-AP5 alters drought stress responses by promoting the degradation of DRIP proteins,Plant Physiol.,175(4):1878-1892
Fracasso A.,Trindade L.M.,and Amaducci S.,2016,Drought stress tolerance strategies revealed by RNA-Seq in two sorghum genotypes with contrasting WUE,BMC Plant Biol.,16(1):115-132
Hamza N.B.,Sharma N.,Tripathi A.,and Sanan-Mishra N.,2016,Micro RNA expression profiles in response to drought stress in Sorghum bicolor,Gene Exp.Patterns,20(2):88-98
Harris K.,Subudhi P.K.,Borrell A.,Jordan D.,Rosenow D.Nguyen H.,Klein P.,Klein R.,and Mullet J.,2007,Sorghum stay-green QTL individually reduce post-flowering droughtinduced leaf senescence,J.Exp.Bot.,58(2):327-338
He H.,Yan J.,Yu X.Y.,Liang Y.,Fang L.,Scheller H.V.,and Zhang A.Y.,2017,The NADPH-oxidase At Rboh I plays a positive role in drought-stress response in Arabidopsis thaliana,Biochem.Biophys.Res.Commun.,491(3):834-839
Johnson S.M.,Lim F.L.,Finkler A.,Fromm H.,Slabas A.R.,and Knight M.R.,2014,Transcriptomic analysis of Sorghum bicolor responding to combined heat and drought stress,BMCGenomics,15(1):456-474
Katiyar A.,Smita S.,Muthusamy S.K.,Chinnusamy V.,Pandey D.M.,and Bansal K.C.,2015,Identification of nove drought-responsive micro RNAs and trans-acting si RNAs from Sorghum bicolor(L.)Moench by high-throughput sequencing analysis,Front Plant Sci.,6:506-526
Kebede H.,Subudhi P.K.,Rosenow D.T.,and Nguyen H.T.2001,Quantitative trait loci influencing drought tolerance in grain sorghum(Sorghum bicolor L.Moench),Theor.Appl Genet.,103(2-3):266-276
Li S.J.,Zhu J.Q.,Ye X.Y.,Wang L.H.,Song Y.Y.,and Wang HZ.,2005,Double tolerant differences of rice under drough and salt stresses,Xinan Nongye Xuebao(Southwest China Journal of Agricultural Science),18(2):128-132(李姝晋,朱建清,叶小英,王丽华,宋永燕,王贺正,2005,干旱和盐胁迫下水稻品种的双重耐性差异,西南农业学报,18(2)128-132)
Li T.T.,Gao Y.F.,Ma X.,Chen Y.F.,Wang Y.,and Ma J.B.2016,Effects of exogenous brassinosteroid on photosynthesis of three spesies of Populus under drought,salt and copper stress,Jiyinzuxue Yu Yingyong Shengwuxue(Genomics and Applied Biology),36(1):218-226(李涛涛,高永峰,马瑄,陈永富,王阳,马金彪,2016,外源油菜素内酯对三种杨树在干旱、盐和铜胁迫下光合生理的影响,基因组学与应用生物学,36(1):218-226)
Li Z.Y.,Long R.C.,Zhang T.J.,Wang Z.,Zhang F.,Yang Q.C.Kang J.M.,and Sun Y.,2017,Molecular cloning and functional analysis of the drought tolerance gene Ms HSP70 from alfalfa(Medicago sativa L.),J.Plant Res.,130(2):387-396
Liang X.H.,Yi Z.B.,and Zhao W.J.,2005,Research advance on mapping the important resistant-genes of sorghum,Zuowu Zazhi(Crops),(3):7-9(梁小红,仪治本,赵威军,2005,高粱重要抗性性状的基因定位研究综述,作物杂志,(3):7-9)
Liu Z.,2008,Study and appraisal of salt tolerance drought resistance capacity of different alfalfa cultivar,Thesis for M.S.Jilin Agricultural University,Supervisor:Xu A.K.,pp.37-40(刘卓,2008,不同苜蓿品种性、抗旱性比较的研究,硕士学位论文,吉林农业大学,导师:徐安凯,pp.37-40)
Lv X.,Zhang F.Y.,Ping J.A.,Du Z.H.,Li H.M.,Tian Z.X.,Yang T.T.,and Qiao J.,2013,Correlation analysis of 25 main agronomic characters and drought resistance in forage sorghum restorer lines,Zhongguo Nongxue Tongbao(Chinese Agricultural Science Bulletin),29(29):6-13(吕鑫,张福耀,平俊爱,杜志宏,李慧明,田兆祥,杨婷婷,乔婧,2013,25个饲草高粱恢复系主要农艺性状及其抗旱性的相关分析,中国农学通报,29(29):6-13)
Mao H.D.,Wang H.W.,Liu S.X.,Li Z.G.,Yang X.H.,Yan J.B.,Li J.S.,Tran L.S.P.,and Qin F.,2015,A transposable element in a NAC gene is associated with drought tolerance in maize seedlings,Nat.Commun.,6:8326-8339
Menezes C.B.,Ticona-Benavente C.A.,Tardin F.D.,Cardoso M.J.,Bastos E.A.,Nogueira D.W.,Portugal A.F.,Santos C.V.,and Schaffert R.E.,2014,Selection indices to identify drought-tolerant grain sorghum cultivars,Genet.Mol.Res.,13(4):9817-9827
Paterson A.H.,Bowers J.E.,Bruggmann R.,Dubchak I.,Grimwood J.,Gundlach H.,Haberer G.,Hellsten U.,Mitros T.,Poliakov A.,Schmutz J.,Spannagl M.,Tang H.B.,Wang X.Y.,Wicker T.,Bharti A.K.,Chapman J.,Feltus F.A.,Gowik U.,Grigoriev I.V.,Lyons E.,Maher C.A.,Martis M.,Narechania A.,Otillar R.P.,Penning B.W.,Salamov A.A.,Wang Y.,Zhang L.F.,Carpita N.C.,Freeling M.,Gingle A.R.,Hash C.T.,Keller B.,Klein P.,Kresovich S.,Mc Cann M.C.,Ming R.,Peterson D.G.,Mehboob-ur-Rahman,Ware D.,Westhoff P.,Mayer K.F.X.,Messing J.,and Rokhsar D.S.,2009,The sorghum bicolor genome and the diversification of grasses,Nature,457(7229):551-556
Rama Reddy N.R.,Ragimasalawada M.,Sabbavarapu M.M.,Nadoor S.,and Patil J.V.,2014,Detection and validation of stay-green QTL in post-rainy sorghum involving widely adapted cultivar,M35-1 and a popular stay-green genotype B35,BMC Genomics,15(1):909-924
Tuinstra M.R.,Grote E.M.,Goldsbrough P.B.,and Ejeta G.,1996,Identification of quantitative trait loci associated with pre-flowering drought tolerance in sorghum,Crop Sci.,36(5):1337-1344
Wu Q.,Zhou Y.F.,Gao Y.,Zhang J.,Chen B.R.,Xu W.J.,and Huang R.D.,2016,Screening and identification for drought resistance during germination in sorghum cultivars,Zuowu Xuebao(Acta Agronomica Sinica),42(8):1233-1246(吴奇,周宇飞,高悦,张姣,陈冰嬬,许文娟,黄瑞冬,2016,不同高粱品种萌发期抗旱性筛选与鉴定,作物学报,42(8):1233-1246)
Xu W.W.,Subudhi P.K.,Crasta O.R.,Rosenow D.T.,Mullet J.E.,and Nguyen H.T.,2000,Molecular mapping of QTLs conferring stay-green in grain sorghum(Sorghum bicolor L.Moench),Genome,43(3):461-469
Yang F.,Wei X.C.,Zhang S.C.,and Wang B.S.,2015,Comparison on salt and drought resistances of different varieties of Sorghum bicolor at germination stage,Zhiwu Shengli Xue bao(Plant Physiology Journal),51(10):1604-1610(杨帆,魏晓岑,张士超,王宝山,2015,不同甜高粱品种萌发期抗盐和抗旱性比较,植物生理学报,51(10):1604-1610)
Yi Z.B.,Liang X.H.,Sun Y.,Li B.L.,Wang J.X.,Zhao W.J.,and Lv H.Q.,2003,Research advance on mapping quantitative trait loci of drought resistance in sorghum,Zaliang Zuowu(Rain Fed Crops),23(5):262-268(仪治本,梁小红,孙毅李炳林,王景雪,赵威军,吕慧卿,2003,高粱抗旱性数量性状位点基因定位研究进展,杂粮作物,23(5):262-268)
Zhang C.,Li C.,Liu J.,Lv Y.,Yu C.,Li H.,Zhao T.,and Liu B.,2017a,The Os ABF1 transcription factor improves drought tolerance by activating the transcription of COR413-TM1 in rice,J.Exp.Bot.,68(16):4695-4707
Zhang N.,Yin Y.J.,Liu X.Y.,Tong S.M.,Xing J.W.,Zhang Y.,Pudake R.N.,Izquierdo E.M.,Peng H.,Xin M.M.,Hu Z.R.,Ni Z.F.,Sun Q.X.,and Yao Y.Y.,2017b,The E3 ligase Ta S-AP5 alters drought stress responses by promoting the degradation of DRIP proteins,Plant Physiol.,175(4):1878-1892