高粱种子萌发期耐盐材料的筛选与鉴定
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摘要
【目的】种子萌发期,筛选、鉴定耐盐材料对保证高粱群体数量、高粱产量以及培育高粱耐盐品种都具有重要意义。【方法】首先以粒用高粱BTx623和甜高粱Rio为代表,采用Na Cl模拟盐胁迫,设置0(CK),0. 2%、0. 4%、0. 6%、0. 8%、1. 0%、1. 2%、1. 4%、1. 6%、1. 8%和2. 0%不同浓度梯度处理,确定Na Cl初步筛选浓度。其次,利用Na Cl初步筛选浓度对全球收集的396份高粱进行初步筛选,筛选出较耐盐的材料逐渐加大选择压力,最终筛选出强耐盐材料。【结果】经过以上试验,确定了大批材料的初步筛选时Na Cl浓度为1. 2%较为适宜。396份高粱材料经过1. 2%、1. 4%、1. 6%、1. 8%逐步筛选、鉴定,最终筛选出5份耐盐能力很强的高粱材料,其中4份是中国的粒用高粱,1份是美国的粒用高粱。【结论】高粱种子萌发期,对大量种质资源进行耐盐材料筛选时,需要确定较为适宜的Na Cl筛选浓度以及需要逐级加大选择压力。
【Objective】At the seed germination stage of sorghum,screening and identification of the salt-resistant materials,are important for assuring crop population,yield and cultivating the salt-resistant varieties. 【Method】Firstly,grain sorghum BTx623 and sweet sorghum Rio,were separately treated with 0(CK),0. 2 %,0. 4 %,0. 6 %,0. 8 %,1. 0 %,1. 2 %,1. 4 %,1. 6 %,1. 8 % and 2. 0 % Na Cl,which was aimed at determining the initial screening concentration of Na Cl. Secondly,396 sorghum materials,from the whole world,were treated by the Na Cl initial screening concentration to screen the salt-resistant materials,and then gradually increase selection pressure to screen the stronger salt-resistant materials. 【Result】Our data showed that 1. 2 % Na Cl was appropriate for initial screening for a large number of materials. 396 sorghum materials were treated by 1. 2 %,1. 4 %,1. 6 % and 1. 8 % Na Cl stepwise selection,5 higher salt-resistant materials were obtained,4 materials were from China,1 material was from America,which were all grain sorghum. 【Conclusion】At the seed germination stage of sorghum,we need determining the appropriate screening concentration of Na Cl and gradually increasing the selection pressure during screening and identification of the salt-resistant materials.
【Objective】At the seed germination stage of sorghum,screening and identification of the salt-resistant materials,are important for assuring crop population,yield and cultivating the salt-resistant varieties. 【Method】Firstly,grain sorghum BTx623 and sweet sorghum Rio,were separately treated with 0(CK),0. 2 %,0. 4 %,0. 6 %,0. 8 %,1. 0 %,1. 2 %,1. 4 %,1. 6 %,1. 8 % and 2. 0 % Na Cl,which was aimed at determining the initial screening concentration of Na Cl. Secondly,396 sorghum materials,from the whole world,were treated by the Na Cl initial screening concentration to screen the salt-resistant materials,and then gradually increase selection pressure to screen the stronger salt-resistant materials. 【Result】Our data showed that 1. 2 % Na Cl was appropriate for initial screening for a large number of materials. 396 sorghum materials were treated by 1. 2 %,1. 4 %,1. 6 % and 1. 8 % Na Cl stepwise selection,5 higher salt-resistant materials were obtained,4 materials were from China,1 material was from America,which were all grain sorghum. 【Conclusion】At the seed germination stage of sorghum,we need determining the appropriate screening concentration of Na Cl and gradually increasing the selection pressure during screening and identification of the salt-resistant materials.
引文
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[13]籍贵苏,杜瑞恒,刘国庆,等.高粱耐盐性评价方法研究及耐盐碱资源的筛选[J].植物遗传资源学报,2013,14(1):25-30.
[14]张云华,孙守均,王云,等.高粱萌发期和苗期耐盐性研究[J].内蒙古民族大学学报(自然科学版),2004,19(3):300-302.
[15]Franwis L E,Donovan J and Mass E V. Salinity effects on seed yield,growth and germination of grain sorghum[J]. Agron J,1984,76(5):741-744.
[16]高凤菊,曹鹏鹏,王乐政,等.盐度对不同类型甜高粱品种萌发的影响[J].山东农业科学,2011,11:44-47.
[17]孙璐,周宇飞,汪澈,等.高粱品种萌发期耐盐性筛选与鉴定[J].中国农业科学2012,45(9):1714-1722.
[2]Chen Z C,Yamaji N,Horie T,et al. A magnesium transporter OsMGT1 plays a critical role in salt tolerance in rice[J]. Plant Physiol,2017,174(3):1837-1849.
[4]Ali Q,Daud M K,Haider M Z,et al. Seed priming by sodium nitroprusside improves salt tolerance in wheat(Triticum aestivum L.)by enhancing physiological and biochemical parameters[J]. Plant Physiol Biochem,2017,119:50-58.
[5]Puranik S,Bahadur R P,Srivastava P S,et al. Molecular cloning and characterization of a membrane associated NAC family gene,SiNAC from foxtail millet[Setaria italica(L.)P. Beauv][J]. Mol Biotechnol,2011,49(2):138-150.
[6]Paterson A H,Bowers J E,Bruggmann R,et al. The sorghum bicolor genome and the diversification of grasses[J]. Nature,2009,457(7229):551-556.
[7]Sui N,Yang Z,Liu M,et al. Identification and transcriptomic profiling of genes involved in increasing sugar content during salt stress in sweet sorghum leaves[J]. BMC Genomics,2015,16:534-551.
[8]Almodares A,Hadi M R,Kholdebarin B,et al. The response of sweet sorghum cultivars to salt stress and accumulation of Na+,Cland K+ions in relation to salinity[J]. J Environ Biol,2014,35(4):733-739.
[9]Wang T T,Ren Z J,Liu Z Q,et al. Sb HKT1; 4,a member of the high-affinity potassium transporter gene family from Sorghum bicolor,functions to maintain optimal Na+/K+balance under Na+stress[J]. J Integr Plant Biol,2014,56(3):315-332.
[10]Anjaneyulu E,Reddy P S,Sunita M S,et al. Salt tolerance and activity of antioxidative enzymes of transgenic finger millet overexpressing a vacuolar H(+)-pyrophosphatase gene(Sb VPPase)from Sorghum bicolor[J]. J Plant Physiol,2014,171(10):789-798.
[11]Su M,Li X F,Ma X Y,et al. Cloning two P5CS genes from bioenergy sorghum and their expression profiles under abiotic stresses and Me JA treatment[J]. Plant Sci,2011,181(6):652-659.
[12]Wang H,Chen G,Zhang H,et al. Identification of QTLs for salt tolerance at germination and seedling stage of Sorghum bicolor L.Moench[J]. Euphytica,2014,196(1):117-127.
[13]籍贵苏,杜瑞恒,刘国庆,等.高粱耐盐性评价方法研究及耐盐碱资源的筛选[J].植物遗传资源学报,2013,14(1):25-30.
[14]张云华,孙守均,王云,等.高粱萌发期和苗期耐盐性研究[J].内蒙古民族大学学报(自然科学版),2004,19(3):300-302.
[15]Franwis L E,Donovan J and Mass E V. Salinity effects on seed yield,growth and germination of grain sorghum[J]. Agron J,1984,76(5):741-744.
[16]高凤菊,曹鹏鹏,王乐政,等.盐度对不同类型甜高粱品种萌发的影响[J].山东农业科学,2011,11:44-47.
[17]孙璐,周宇飞,汪澈,等.高粱品种萌发期耐盐性筛选与鉴定[J].中国农业科学2012,45(9):1714-1722.