食源性动物组织中β-受体激动剂研究进展
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摘要
β-受体激动剂是一类苯乙醇胺类药物,化学结构和生理功能类似于肾上腺素和去甲肾上腺素,常用于防治人和动物支气管哮喘等疾病。由于β-受体激动剂类药物可促进动物体内蛋白质合成并抑制脂肪沉淀,使动物体内营养成分再分配,所以此类药物常被添加到动物饲养过程中用于提高瘦肉率,继而造成药物在动物体内残留,人类食用后会引发急性中毒而威胁健康。虽然大多数国家已经明令禁止在动物饲养中使用此类药物,但是仍然存在非法使用的情况。为了加强动物源性食品安全,各国已经展开了对β-受体激动剂类药物的研究,已建立多种快速准确的检测方法。本研究主要对β-受体激动剂的结构和性质、在动物源性食品中代谢消除规律和β-受体激动剂检测技术3个方面进行阐述,并对β-受体激动剂药物残留检测的未来研究方向进行展望。
β-agonists are a class of phenylethanolamines with chemical structures and physiological functions similar to epinephrine and norepinephrine. They are commonly used to prevent diseases such as bronchial asthma in humans and animals. Since β-agonists can promote protein synthesis and inhibit fat deposition in animals, and redistribute nutrients in animals, such drugs are often added to animal feeding processes to increase lean meat rate, which in turn leads to drugs residue in animals, causing acute poisoning and threatening health after human consumption. Although most countries have explicitly banned the use of such drugs in animal husbandry, there are still cases of illegal use. In order to enhance the safety of animal-derived foods, countries have begun research on β-agonist agonists, and a variety of rapid and accurate detection methods have been established. This article summarized the structure and properties, the metabolic elimination rule in animal-derived foods and the detection technology of β-agonists, and prospected the future research direction of β-agonist drug residue detection.
β-agonists are a class of phenylethanolamines with chemical structures and physiological functions similar to epinephrine and norepinephrine. They are commonly used to prevent diseases such as bronchial asthma in humans and animals. Since β-agonists can promote protein synthesis and inhibit fat deposition in animals, and redistribute nutrients in animals, such drugs are often added to animal feeding processes to increase lean meat rate, which in turn leads to drugs residue in animals, causing acute poisoning and threatening health after human consumption. Although most countries have explicitly banned the use of such drugs in animal husbandry, there are still cases of illegal use. In order to enhance the safety of animal-derived foods, countries have begun research on β-agonist agonists, and a variety of rapid and accurate detection methods have been established. This article summarized the structure and properties, the metabolic elimination rule in animal-derived foods and the detection technology of β-agonists, and prospected the future research direction of β-agonist drug residue detection.
引文
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[3]李阳,苏晓鸥,王瑞国,等.超高效液相色谱-串联质谱法同时测定绵羊唾液中14种β-受体激动剂[J].分析化学,2013,41(6):899-904.Li Y,Su XO,Wang RG,et al.Simultaneous determination of 14 kinds ofβ-agonists residues in sheep saliva by ulta performance liquid chramatogrephy-tandem mass spectrometry[J].Chin J Anal Chem,2013,41(6):899-904.
[4]Juan C,Igualada C,Moragues F,et al.Development and validation of a liquid chromatography tandem mass spectrometry method for the analysis ofβ-agonists in animal feed and drinking water[J].J Chromatogr A,2010,1217(39):6061-6068.
[5]韩婉清,吴楚森,吴玉銮,等.超高效液相色谱-串联质谱测定动物肌肉组织中32种β-激动剂、β-阻滞剂和糖肽类抗生素药物残留[J].分析化学,2016,44(2):289-296.Han WQ,Wu CS,Wu YL,et al.Analysis of 32 kinds ofβ-agonists,β-blockers and glycopeptide antibiotics in animal meat by UPLC-MS/MS[J].Chin J Anal Chem,2016,44(2):289-296.
[6]宁霄,金绍明,梁瑞强,等.超高效液相色谱-串联质谱法测定猪肉中β-受体激动剂残留量的不确定度分析[J].食品科学,2015,38(6):315-320.Ning X,Jin SM,Liang RQ,et al.Uncertainty evaluation for the detcetion ofβ-agonists in pork by ultra performance liquid chromatography-tandem mass spectrometry[J].Food Sci,2015,38(6):315-320.
[7]Zhang ZH,Yan H,Cui FY,et al.Analysis of multipleβ-Agonist andβ-blocker residues in porcine muscle using improved QuEChERS method and UHPLC-LTQ orbitrap mass spectrometry[J].Food Anal Method,2016,9(4):915-924.
[8]Leporati M,Bergoglio M,Capra P,et al.Development,validation and application to real samples of a multiresidue LC-MS/MS method for determination ofβ2-agonists and anabolic steroids in bovine hair[J].JMass Spectrom,2014,49(9):936-946.
[9]Sung IK,Park SJ,Karig K,et al.Development and application of a method for rapid and simultaneous determination of threeβ-agonists(clenbuterol,ractopamine,and zilpaterol)using liquid chromatography-tandem mass spectrometry[J].Korean J Food Sci Anal,2015,35(1):121-129.
[10]孙晓亮,李雪莲,曹旭敏,等.超高效液相色谱-串联质谱法快速测定猪尿液中30种不同种类“瘦肉精”药物残留[J].分析化学,2017,45(1):124-132.Sun XL,Li XL,Cao XM,et al.An ultra-high performance liquid chromatography-tandem mass spectrometric method for fast determination of 30 diffierent species of lean meat agents residues in pig urine[J].Chin JAnal Chem,2017,45(1):124-132.
[11]熊琳,李维红,高雅琴,等.肉品中β-受体激动剂类药物残留检测技术研究进展[J].食品安全质量检测学报,2015,6(2):528-533.Xiong L,Li WH,Gao YQ,et al.A review of inspection technology ofβ-agonists drug residues in meat[J].J Food Saf Qual,2015,6(2):528-533.
[12]彭涛,赖卫华,张富生,等.20种β2-受体激动剂的性质及检测方法研究进展[J].食品与机械,2013,29(3):254-260.Peng T,Lai WH,Zhang FS,et al.Research progress of properties and detection methods of 20 kinds ofβ2-agonists[J].Food Mach,2013,29(3):254-260.
[13]王培龙.β-受体激动剂及其检测技术研究[J].农产品质量与安全,2014,(1):44-52.Wang PL.Rserach onβ-agonists’detections technologies[J].Qual Saf Agro-Prod,2014,(1):44-52.
[14]Commission regulation(EU)No 37/2010.On pharmacologically active substances and their classification regarding maximum residue in foodstuffs of animal origin[S].
[15]Codex Alimentarius Commission.Maximum residue limits(MRL)risk management recommendations(RMRs)for residues of veterinary drugs in foods[S].
[16]农业部第176号公告禁止在饲料和动物饮用水中使用的物质名单[S].Notice No.176th Ministry of Agriculture.List of substances prohibited from use in feed and animal drinking water[S].
[17]农业部第1519号公告禁止在饲料和动物饮用水中使用的物质名单[S].Notice No.1519th Ministry of Agriculture.List of substances prohibited from use in feed and animal drinking water[S].
[18]袁飞,张峰.解读我国饲料中违禁药物添加管理法规[J].饲料工业,2013,34(15):61-64.Yuan F,Zhang F.Interpretation of regulations on administration of prohibited drugs in feed[J].Feed Ind,2013,34(15):61-64.
[19]刘士杰.盐酸克伦特罗在动物组织中的残留分布及代谢规律[D].北京:中国农业科学院,2006.Liu SJ.The residues and depletion of clenbuterol in animal tissues[D].Beijing:Chinese Academy of Agricultural Sciences Institute,2006.
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[21]Churchwell MI,Holder CL,Little D,et al.Liquid chromatography/electrospray tandem mass spectrometric analysis of incurred ractopamine residues in livestock tissues[J].Rapid Comm Mass Spectrom,2002,16(13):1261-1265.
[22]卢艳芬,徐丽君,王辉,等.超高效液相色谱-串联质谱法同时测定饲料中的7种β2-兴奋剂[J].质谱学报,2012,33(5):280-285.Lu YF,Xu LJ,Wang H,et al.Simultaneous determination of 7 kinds ofβ2-agonists in feed by UPLC-MS/MS[J].J Chin Mass Spectr Soc,2012,33(5):280-285.
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