妊娠早期动物体液外泌体miRNA的研究进展
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摘要
外泌体(exosomes)广泛存在于动物的唾液、血液、尿液及乳汁等体液中。大量研究表明,miRNA是一类长度为18～25 nt的内源性非编码RNA,其在不同样本中表达呈现独特的miRNA指纹,可以作为理想的生物标志物。了解外泌体miRNA的分子作用机制,对动物早期妊娠诊断和治疗具有重要意义。此外,外泌体miRNA参与了生物的生长发育、细胞增殖分化、凋亡、糖脂代谢、神经元形成、造血、妊娠以及疾病发生过程的调节。本研究总结了近几年外泌体miRNA的研究进展,对了解母畜繁殖调控有着重要意义。
Exosomes are found in animal saliva,blood and urine,milk,etc.Most studies have shown that miRNAs are endogenous non-coding RNA of 18-25 nt in length,and its expression according to different samples shows differences of unique miRNA fingerprint.Understanding the molecular mechanism of exosomes miRNAs plays an important role in the diagnosis and treatment of early pregnancy.Moreover,Exosomes miRNAs are involved in the growth and development of organisms,cell proliferation and differentiation,apoptosis,glucose and lipid metabolism,neuronal formation,hematopoiesis,pregnancy,and disease progression.This article reviews the recent advances in the study of exosomes miRNA which had great significance to understand the regulation of female breeding.
Exosomes are found in animal saliva,blood and urine,milk,etc.Most studies have shown that miRNAs are endogenous non-coding RNA of 18-25 nt in length,and its expression according to different samples shows differences of unique miRNA fingerprint.Understanding the molecular mechanism of exosomes miRNAs plays an important role in the diagnosis and treatment of early pregnancy.Moreover,Exosomes miRNAs are involved in the growth and development of organisms,cell proliferation and differentiation,apoptosis,glucose and lipid metabolism,neuronal formation,hematopoiesis,pregnancy,and disease progression.This article reviews the recent advances in the study of exosomes miRNA which had great significance to understand the regulation of female breeding.
引文
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[2] BARTEL D P.Micrornas:target recognition and regulatory functions[J].Cell,2009,136(2):215-233.
[3] GHAI V,WANG K.Recent progress toward the use of circulating micrornas as clinical biomarkers[J].Arch Toxicol,2016,90(12):2959-2978.
[4] 徐源,孙铁成,张爱玲,等.卵巢 miRNA 研究进展[J].畜牧兽医学报,2014,45(4):509-516.
[5] 吴金恩,郑亚东,孔贺磊,等.感染绵羊痘病毒的绵羊睾丸细胞外泌体源性蛋白组学分析[J].中国兽医学报,2018,38(2):259-265.
[6] 章杰,柴捷,陈磊,等.猪血清和血浆外泌体miRNA的鉴定与比较[J].中国兽医学报,2018,38(4):795-808.
[7] EGAWA N,KOSHIKAWA N,TOMARI T,et al.Membrane type 1 matrix metalloproteinase (mt1-mmp/mmp-14) cleaves and releases a 22-kda extracellular matrix metalloproteinase inducer (emmprin) fragment from tumor cells[J].J Biol Chem,2006,281(49):37576-37585.
[8] PAN B,JOHNSTONE R M.Fate of the transferrin receptor during maturation of sheep reticulocytes in vitro:selective externalization of the receptor[J].Cell,1983,33(3):967-978.
[9] THéRY C,REGNAULT A,GARIN J,et al.Molecular characterization of dendritic cell-derived exosomes:selective accumulation of the heat shock protein Hsc73[J].J Cell Biol,1999,147(3):599-610.
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[12] ADMYRE C,TELEMO E,ALMQVIST N,et al.Exosomes-nanovesicles with possible roles in allergic inflammation[J].Allergy,2008,63(4):404-408.
[13] MEARS R,CRAVEN RA,HANRAHAN S,et al.Proteomic analysis of melanoma-derived exosomes by two-dimensional polyacrylamide gel electrophoresis and mass spectrometry[J].Proteomics,2004,4(12):4019-4031.
[14] 孙默萱,王洪江.血清miRNA的表达与肿瘤诊断的研究进展[J].中国医学前沿杂志,2012,4(5):72-75.
[15] VALADI H,EKSTR?M K,BOSSIOS A,et al.Exosome-mediated transfer of mrnas and micrornas is a novel mechanism of genetic exchange between cells[J].Nature Cell Biol,2007,9(6):654.
[16] HUANG X,YUAN T,LIANG M,et al.Exosomal Mir-1290 and Mir-375 as prognostic markers in castration-resistant prostate cancer[J].Eur Urol,2015,67(1):33-41.
[17] MITCHELL PS,PARKIN R K,KROH EM,et al.Circulating micrornas as stable blood-based markers for cancer detection[J].P Natl Acad Sci,2008,105(30):10513-10518.
[18] WEBER J A,BAXTER D H,ZHANG S,et al.The microRNA spectrumin 12 body fluids[J].Clin Chem,2010,56(11):1733-1741.
[19] XIE Z,CHEN G,ZHANG X,et al.Saliva supernatant Mir-21:a novel potential biomarker for esophageal cancer detection[J].Asian Pac J Cancer P,2012,13(12):6145-6149.
[20] ZHENG D,HADDADIN S,WANG Y,et al.Plasma micrornas as novel biomarkers for early detection of lung cancer[J].Int J Clin Exp Pathol,2011,4(6):575-586.
[21] XIE Z,YIN X,GONG B,et al.Salivary micrornas show potential as a noninvasive biomarker for detecting resectable pancreatic cancer[J].Cancer Prev Res,2015,8(2):165-173.
[22] ZHU H.MicroRNA functions in animal development and human disease[J].Development,2011,132:4653-4662.
[23] KOTLABOVA K,DOUCHA J,HROMADNIKOVA I.Placental-specific microrna in maternal circulation-identification of appropriate pregnancy-associated micrornas with diagnostic potential[J].Am J Reprod Immunol,2011,89(2):185-191.
[24] IOANNIDIS J,DONADEU F X.Circulating mirna signatures of early pregnancy in cattle[J].BMC Genom,2016,17(1):184.
[25] KLOHONATZ K M,CAMERON A D,HERGENREDER J R,et al.Circulating mirnas as potential alternative cell signaling associated with maternal recognition of pregnancy in the mare[J].Biol Reprod,2016,95(6):124.
[26] POHLER K G,GREEN J A,MOLEY L A,et al.Circulating microrna as candidates for early embryonic viability in cattle[J].Mol Reprod Dev,2017,84(8):731-743.
[27] CLEYS E R,HALLERAN J L,MCWHORTER E,et al.Identification of micrornas in exosomes isolated from serum and umbilical cord blood,as well as placentomes of gestational day 90 pregnant sheep[J].Mol Reprod Dev,2014,81(11):983-993.
[28] BRASE J C,JOHANNES M,SCHLOMM T,et a1.Circulating miRNAs are correlated with tumor progression in prostate cancer[J].Int J Cancer,201l,128:608-616.
[29] SILVEIRA D A,ANDRADE G M,PERECIN F,et al.Microrna protocols[M].Springer,2018:53-63.
[30] WANG Y,HU T,WU L,et al.Identification of non-coding and coding rnas in porcine endometrium[J].Genomics,2017,109(1):43-50.
[31] SU L,LIU R,CHENG W,et al.Expression patterns of micrornas in porcine endometrium and their potential roles in embryo implantation and placentation[J].PLoS One,2014,9(2):e87867.
[32] CHAKRABARTY A,TRANGUCH S,DAIKOKU T,et al.Microrna regulation of cyclooxygenase-2 during embryo implantation[J].Proc Natl Acad Sci,2007,104(38):15144-15149.
[33] KRAWCZYNSKI K,NAJMULA J,BAUERSACHS S,et al.Micrornaome of porcine conceptuses and trophoblasts:expression profile of micrornas and their potential to regulate genes crucial for establishment of pregnancy[J].Biol Reprod,2015,92(1):21.
[34] BURNS G,BROOKS K,WILDUNG M,et al.Extracellular vesicles in luminal fluid of the ovine uterus[J].PLos One,2014,9(3):e90913.
[35] LI W,XI Y,XUE S,et al.Sequence analysis of micrornas during pre-implantation between meishan and yorkshire pigs[J].Gene,2017,646:20-27.
[36] HAN W,ZHU Y,SU Y,et al.High-throughput sequencing reveals circulating mirnas as potential biomarkers for measuring puberty onset in chicken (gallus gallus)[J].PLoS One,2016,11(5):e0154958.
[37] 童超.外泌体miRNA和lncRNA作为奶牛乳腺炎分子标记的筛选和功能鉴定[D].陕西杨凌:西北农林科技大学,2017.
[38] ZHENG Y,CHEN K,ZHENG X,et al.Identification and bioinformatics analysis of micrornas associated with stress and immune response in serum of heat-stressed and normal holstein cows[J].Cell Stress Chaperon,2014,19(6):973-981.
[39] SALOMON C,TORRES M J,KOBAYASHI M,et al.A gestational profile of placental exosomes in maternal plasma and their effects on endothelial cell migration[J].PLoS One,2014,9(6):e98667.
[40] GO A T,VAN J M,OUDEJANS C B.Non-invasive aneuploidy detection using free fetal dna and rna in maternal plasma:recent progress and future possibilities[J].Hum Reprod Update,2010,17(3):372-382.
[41] CHOI S,YUN J,LEE O,et al.Microrna expression profiles in placenta with severe preeclampsia using a pna-based microarray[J].Placenta,2013,34(9):799-804.
[42] YU Y,WANG L,LIU T,et al.Microrna-204 suppresses trophoblast-like cell invasion by targeting matrix metall oproteinase-9[J].Biochem Bioph Res Co,2015,463(3):285-291.
[43] OUYANG Y,MOUILLET J,COYNE C B,et al.Placenta-specific micrornas in exosomes-good things come in nano-packages[J].Placenta,2014,35:S69-73.
[44] INUI M,MARTELLO G,PICCOLO S.Microrna control of signal transduction[J].Nat Rev Mol Cell Bio,2010,11(4):252.
[45] BAEK D,VILLéN J,SHIN C,et al.The impact of micrornas on protein output[J].Nature,2008,455(7209):64.
[2] BARTEL D P.Micrornas:target recognition and regulatory functions[J].Cell,2009,136(2):215-233.
[3] GHAI V,WANG K.Recent progress toward the use of circulating micrornas as clinical biomarkers[J].Arch Toxicol,2016,90(12):2959-2978.
[4] 徐源,孙铁成,张爱玲,等.卵巢 miRNA 研究进展[J].畜牧兽医学报,2014,45(4):509-516.
[5] 吴金恩,郑亚东,孔贺磊,等.感染绵羊痘病毒的绵羊睾丸细胞外泌体源性蛋白组学分析[J].中国兽医学报,2018,38(2):259-265.
[6] 章杰,柴捷,陈磊,等.猪血清和血浆外泌体miRNA的鉴定与比较[J].中国兽医学报,2018,38(4):795-808.
[7] EGAWA N,KOSHIKAWA N,TOMARI T,et al.Membrane type 1 matrix metalloproteinase (mt1-mmp/mmp-14) cleaves and releases a 22-kda extracellular matrix metalloproteinase inducer (emmprin) fragment from tumor cells[J].J Biol Chem,2006,281(49):37576-37585.
[8] PAN B,JOHNSTONE R M.Fate of the transferrin receptor during maturation of sheep reticulocytes in vitro:selective externalization of the receptor[J].Cell,1983,33(3):967-978.
[9] THéRY C,REGNAULT A,GARIN J,et al.Molecular characterization of dendritic cell-derived exosomes:selective accumulation of the heat shock protein Hsc73[J].J Cell Biol,1999,147(3):599-610.
[10] MCLELLAN A.Exosome release by primary B cells[J].Crit Rev Immunol,2009,29(3):203-217.
[11] BLANCHARD N,LANKAR D,FAURE F,et al.Tcr activation of human T cells induces the production of exosomes bearing the Tcr/cd3/ζ complex[J].J Immunol,2002,168(7):3235-3241.
[12] ADMYRE C,TELEMO E,ALMQVIST N,et al.Exosomes-nanovesicles with possible roles in allergic inflammation[J].Allergy,2008,63(4):404-408.
[13] MEARS R,CRAVEN RA,HANRAHAN S,et al.Proteomic analysis of melanoma-derived exosomes by two-dimensional polyacrylamide gel electrophoresis and mass spectrometry[J].Proteomics,2004,4(12):4019-4031.
[14] 孙默萱,王洪江.血清miRNA的表达与肿瘤诊断的研究进展[J].中国医学前沿杂志,2012,4(5):72-75.
[15] VALADI H,EKSTR?M K,BOSSIOS A,et al.Exosome-mediated transfer of mrnas and micrornas is a novel mechanism of genetic exchange between cells[J].Nature Cell Biol,2007,9(6):654.
[16] HUANG X,YUAN T,LIANG M,et al.Exosomal Mir-1290 and Mir-375 as prognostic markers in castration-resistant prostate cancer[J].Eur Urol,2015,67(1):33-41.
[17] MITCHELL PS,PARKIN R K,KROH EM,et al.Circulating micrornas as stable blood-based markers for cancer detection[J].P Natl Acad Sci,2008,105(30):10513-10518.
[18] WEBER J A,BAXTER D H,ZHANG S,et al.The microRNA spectrumin 12 body fluids[J].Clin Chem,2010,56(11):1733-1741.
[19] XIE Z,CHEN G,ZHANG X,et al.Saliva supernatant Mir-21:a novel potential biomarker for esophageal cancer detection[J].Asian Pac J Cancer P,2012,13(12):6145-6149.
[20] ZHENG D,HADDADIN S,WANG Y,et al.Plasma micrornas as novel biomarkers for early detection of lung cancer[J].Int J Clin Exp Pathol,2011,4(6):575-586.
[21] XIE Z,YIN X,GONG B,et al.Salivary micrornas show potential as a noninvasive biomarker for detecting resectable pancreatic cancer[J].Cancer Prev Res,2015,8(2):165-173.
[22] ZHU H.MicroRNA functions in animal development and human disease[J].Development,2011,132:4653-4662.
[23] KOTLABOVA K,DOUCHA J,HROMADNIKOVA I.Placental-specific microrna in maternal circulation-identification of appropriate pregnancy-associated micrornas with diagnostic potential[J].Am J Reprod Immunol,2011,89(2):185-191.
[24] IOANNIDIS J,DONADEU F X.Circulating mirna signatures of early pregnancy in cattle[J].BMC Genom,2016,17(1):184.
[25] KLOHONATZ K M,CAMERON A D,HERGENREDER J R,et al.Circulating mirnas as potential alternative cell signaling associated with maternal recognition of pregnancy in the mare[J].Biol Reprod,2016,95(6):124.
[26] POHLER K G,GREEN J A,MOLEY L A,et al.Circulating microrna as candidates for early embryonic viability in cattle[J].Mol Reprod Dev,2017,84(8):731-743.
[27] CLEYS E R,HALLERAN J L,MCWHORTER E,et al.Identification of micrornas in exosomes isolated from serum and umbilical cord blood,as well as placentomes of gestational day 90 pregnant sheep[J].Mol Reprod Dev,2014,81(11):983-993.
[28] BRASE J C,JOHANNES M,SCHLOMM T,et a1.Circulating miRNAs are correlated with tumor progression in prostate cancer[J].Int J Cancer,201l,128:608-616.
[29] SILVEIRA D A,ANDRADE G M,PERECIN F,et al.Microrna protocols[M].Springer,2018:53-63.
[30] WANG Y,HU T,WU L,et al.Identification of non-coding and coding rnas in porcine endometrium[J].Genomics,2017,109(1):43-50.
[31] SU L,LIU R,CHENG W,et al.Expression patterns of micrornas in porcine endometrium and their potential roles in embryo implantation and placentation[J].PLoS One,2014,9(2):e87867.
[32] CHAKRABARTY A,TRANGUCH S,DAIKOKU T,et al.Microrna regulation of cyclooxygenase-2 during embryo implantation[J].Proc Natl Acad Sci,2007,104(38):15144-15149.
[33] KRAWCZYNSKI K,NAJMULA J,BAUERSACHS S,et al.Micrornaome of porcine conceptuses and trophoblasts:expression profile of micrornas and their potential to regulate genes crucial for establishment of pregnancy[J].Biol Reprod,2015,92(1):21.
[34] BURNS G,BROOKS K,WILDUNG M,et al.Extracellular vesicles in luminal fluid of the ovine uterus[J].PLos One,2014,9(3):e90913.
[35] LI W,XI Y,XUE S,et al.Sequence analysis of micrornas during pre-implantation between meishan and yorkshire pigs[J].Gene,2017,646:20-27.
[36] HAN W,ZHU Y,SU Y,et al.High-throughput sequencing reveals circulating mirnas as potential biomarkers for measuring puberty onset in chicken (gallus gallus)[J].PLoS One,2016,11(5):e0154958.
[37] 童超.外泌体miRNA和lncRNA作为奶牛乳腺炎分子标记的筛选和功能鉴定[D].陕西杨凌:西北农林科技大学,2017.
[38] ZHENG Y,CHEN K,ZHENG X,et al.Identification and bioinformatics analysis of micrornas associated with stress and immune response in serum of heat-stressed and normal holstein cows[J].Cell Stress Chaperon,2014,19(6):973-981.
[39] SALOMON C,TORRES M J,KOBAYASHI M,et al.A gestational profile of placental exosomes in maternal plasma and their effects on endothelial cell migration[J].PLoS One,2014,9(6):e98667.
[40] GO A T,VAN J M,OUDEJANS C B.Non-invasive aneuploidy detection using free fetal dna and rna in maternal plasma:recent progress and future possibilities[J].Hum Reprod Update,2010,17(3):372-382.
[41] CHOI S,YUN J,LEE O,et al.Microrna expression profiles in placenta with severe preeclampsia using a pna-based microarray[J].Placenta,2013,34(9):799-804.
[42] YU Y,WANG L,LIU T,et al.Microrna-204 suppresses trophoblast-like cell invasion by targeting matrix metall oproteinase-9[J].Biochem Bioph Res Co,2015,463(3):285-291.
[43] OUYANG Y,MOUILLET J,COYNE C B,et al.Placenta-specific micrornas in exosomes-good things come in nano-packages[J].Placenta,2014,35:S69-73.
[44] INUI M,MARTELLO G,PICCOLO S.Microrna control of signal transduction[J].Nat Rev Mol Cell Bio,2010,11(4):252.
[45] BAEK D,VILLéN J,SHIN C,et al.The impact of micrornas on protein output[J].Nature,2008,455(7209):64.