TLR4基因rs1927914位点多态性与甲状腺疾病的关联分析
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摘要
目的探讨Toll样受体4(TLR4)基因的单核苷酸多态性与Graves病(GD)、桥本甲状腺炎(HT)、单纯性甲状腺肿(SG)的相关性。方法选取2016年河北省沧州市居民甲状腺疾病调查的GD患者(GD组,n=94)、HT患者(HT组,n=54)、SG患者(SG组,n=56)及对照组为研究对象,应用PCR酶切鉴定结合直接测序方法进行基因分型。结果等位基因C和T在3种甲状腺疾病和对照组中比较,差异均无统计学意义(P>0.05);3种基因型CC、CT、TT在GD组、HT组中的分布与对照组比较,差异均无统计学意义(P>0.05);SG组与对照组比较,隐性遗传模型显示基因突变与疾病呈正相关(P=0.040,OR=1.923,95%CI:1.025~3.609)。结论 TLR4基因多态性可能与GD、HT的发病风险无相关性,携带TT基因型可能会增加SG的发病风险。
Objective To investigate the correlation between single nucleotide polymorphism(SNP)of Toll-like receptor 4(TLR4)gene and Graves′disease(GD),Hashimoto′s thyroiditis(HT)or simple goiter(SG).Methods Data was collected from GD patients(the GD group,n=94),HT patients(the HT group,n=54),SG patients(the SG group,n=56),and healthy people(the control group)of 2016 Thyroid Disease Survey in Hebei Cangzhou.Genetic typing was detected by polymerase chain reaction-restriction combined with direct sequencing.Results There was no significant difference in allele C and T at TLR4-C/T between the three thyroid diseases and the control group(P>0.05).The distribution of CC,CT,TT genotypes in the GD group and the HT group was not significantly different from the control group(P>0.05).And by comparing the SG group and the control group,the recessive genetic model showed that gene mutation was positively correlated with the disease(P=0.040,OR=1.923,95%CI:1.025-3.609).Conclusion There might be no correlation between TLR4 gene polymorphism and the incidence of GD or HT,and carrying with the TT genotype may increase the incidence of SG.
Objective To investigate the correlation between single nucleotide polymorphism(SNP)of Toll-like receptor 4(TLR4)gene and Graves′disease(GD),Hashimoto′s thyroiditis(HT)or simple goiter(SG).Methods Data was collected from GD patients(the GD group,n=94),HT patients(the HT group,n=54),SG patients(the SG group,n=56),and healthy people(the control group)of 2016 Thyroid Disease Survey in Hebei Cangzhou.Genetic typing was detected by polymerase chain reaction-restriction combined with direct sequencing.Results There was no significant difference in allele C and T at TLR4-C/T between the three thyroid diseases and the control group(P>0.05).The distribution of CC,CT,TT genotypes in the GD group and the HT group was not significantly different from the control group(P>0.05).And by comparing the SG group and the control group,the recessive genetic model showed that gene mutation was positively correlated with the disease(P=0.040,OR=1.923,95%CI:1.025-3.609).Conclusion There might be no correlation between TLR4 gene polymorphism and the incidence of GD or HT,and carrying with the TT genotype may increase the incidence of SG.
引文
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[12]刘奕婷,王巍,于晓松.幽门螺杆菌感染与甲状腺疾病的相关性研究[J].现代预防医学,2016,43(13):2423-2426.
[13]MORI K,NAKAGAWA Y,OZAKI H.Does the gut microbiota trigger Hashimoto′s thyroiditis?[J].Discov Med,2012,14(78):321-326.
[14]DESAILLOUD R,HOBER D.Viruses and thyroiditis:an update[J].Virol J,2009,6(1):5.
[15]SANTANA N L,RGO J L,OLIVEIRA J M,et al.Polymorphisms in genes TLR1,2and 4are associated with differential cytokine and chemokine serum production in patients with leprosy[J].Mem Inst Oswaldo Cruz,2017,112(4):260-268.
[2]钱晶.健康体检人群甲状腺疾病检出率分析[J].现代预防医学,2014,41(23):4324-4327.
[3]KAWAI T,AKIRA S.The role of pattern-recognition receptors in innate immunity:update on Toll-like receptors[J].Nat Immunoi,2010,11(5):373-384.
[4]MANDREKAR P,PRUETT S,ARTEEL G,et a1.RSA2004:combined basic research satellite symposium-session two:toll-like receptors and organ damage[J].Alcohol Clin Exp Res,2005,29(9):1744-1748.
[5]徐翔,那娜,潘旭东,等.TLR4基因多态性与大动脉粥样硬化性脑梗死及血管床选择性的关联分析[J].中华医学遗传学杂志,2014,31(4):455-461.
[6]梁宝云.TLR4基因多态性与缺血性脑卒中及其中医证型遗传易感性的关联研究[D].南宁:广西中医药大学,2015.
[7]张丽菊.TLR4介导的信号通路基因多态性与2型糖尿病遗传易感性的关联研究[D].广州:暨南大学,2014.
[8]古联,黄静妍,苏莉,等.Toll样受体4基因多态性与汉族男性中风证型关联性研究[J].南京中医药大学学报,2016,32(6):513-518.
[9]SINGH K,SINGH V K,AGRAWAL N K,et al.Association of Toll-like receptor 4polymorphisms with diabetic foot ulcers and application of artificial neural network in DFU risk assessment in type 2diabetes patients[J].Biomed Res Int,2013,2013(2):318686.
[10]SINGH K,KANT S,SINGH V K,et al.Toll-like receptor 4polymorphisms and their haplotypes modulate the risk of developing diabetic retinopathy in type 2diabetes patients[J].Mol Vis,2014,20(5):704-713.
[11]WENG P H,HUANG Y L,PAGE J H,et al.Polymorphisms of an innate immune gene,toll-like receptor 4,and aggressive prostate cancer risk:a systematic review and Meta-analysis[J].PLoS One,2014,9(10):e110569.
[12]刘奕婷,王巍,于晓松.幽门螺杆菌感染与甲状腺疾病的相关性研究[J].现代预防医学,2016,43(13):2423-2426.
[13]MORI K,NAKAGAWA Y,OZAKI H.Does the gut microbiota trigger Hashimoto′s thyroiditis?[J].Discov Med,2012,14(78):321-326.
[14]DESAILLOUD R,HOBER D.Viruses and thyroiditis:an update[J].Virol J,2009,6(1):5.
[15]SANTANA N L,RGO J L,OLIVEIRA J M,et al.Polymorphisms in genes TLR1,2and 4are associated with differential cytokine and chemokine serum production in patients with leprosy[J].Mem Inst Oswaldo Cruz,2017,112(4):260-268.