三种果蔬发酵液抗氧化和免疫调节功能研究
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摘要
对比研究A、B、C三种果蔬发酵液产品的抗氧化能力和免疫调节能力。采用偶氮二异丁脒盐酸盐(AAPH)诱导的HepG2细胞氧化应激模型评价三种发酵液产品对细胞内活性氧簇(ROS)的影响,应用刀豆蛋白A(ConA)和脂多糖(LPS)诱导小鼠T/B淋巴细胞,检测其增殖能力,应用LPS诱导小鼠腹腔巨噬细胞,检测其吞噬能力和NO释放量。结果表明:三种果蔬发酵液具有较强的降低细胞内ROS含量的能力,果蔬发酵液A可使胞内ROS含量降低至55%;三种果蔬发酵液可提高淋巴细胞的增殖能力和腹腔巨噬细胞的吞噬能力,促进NO释放,在稀释倍数为104时果蔬发酵液A处理组NO释放量最高(4.06μmol/L)。三种果蔬发酵液具有较好的抗氧化活性和增强免疫的功能。
The antioxidant activity and immunoregulationon of three kinds of fruit and vegetable zymotic fluid, which were named A, B and C, were researched. The effect of the three kinds of fruit and vegetable zymotic fluid on ROS level in cell was evaluated by AAPH-induced in HepG2 cells model. To study the immunomodulatory ability, concanavalin A and lipopolysaccharide were used to induce the proliferation of mouse T/B lymphocytes, and lipopolysaccharide was used to induce mouse peritoneal macrophages, detecting phagocytic ability and NO release. The results showed that the three kinds of fruit and vegetable zymotic fluid had strong ability to eliminate ROS in cells, A fruit and vegetable zymotic fluid can make intracellular ROS content as low as 55%. They can improve lymphocyte proliferation ability, phagocytic ability of peritoneal macrophages and promote NO release, A fruit and vegetable zymotic fluid has a maximum NO release of 4.06 μmol/L at dilution ratio of 104. These results indicated that the three kinds of fruit and vegetable zymotic fluid have good antioxidant activity and enhance immune function.
The antioxidant activity and immunoregulationon of three kinds of fruit and vegetable zymotic fluid, which were named A, B and C, were researched. The effect of the three kinds of fruit and vegetable zymotic fluid on ROS level in cell was evaluated by AAPH-induced in HepG2 cells model. To study the immunomodulatory ability, concanavalin A and lipopolysaccharide were used to induce the proliferation of mouse T/B lymphocytes, and lipopolysaccharide was used to induce mouse peritoneal macrophages, detecting phagocytic ability and NO release. The results showed that the three kinds of fruit and vegetable zymotic fluid had strong ability to eliminate ROS in cells, A fruit and vegetable zymotic fluid can make intracellular ROS content as low as 55%. They can improve lymphocyte proliferation ability, phagocytic ability of peritoneal macrophages and promote NO release, A fruit and vegetable zymotic fluid has a maximum NO release of 4.06 μmol/L at dilution ratio of 104. These results indicated that the three kinds of fruit and vegetable zymotic fluid have good antioxidant activity and enhance immune function.
引文
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[2]任清,于晓艳,潘妍,等.微生物酵素美白抗衰老功效研究[J].香料香精化妆品,2008(3):28-32.
[3]曲佳乐,赵金凤,皮子凤,等.植物发酵液解酒护肝保健功能研究[J].食品科技,2013,38(9):51-55.
[4]赵金凤,曲佳乐,皮子凤,等.植物发酵液润肠通便保健功能研究[J].食品与发酵科技,2012,48(3):54-56.
[5]潘宣如,毛旸昊.浙江果蔬发酵液新兴产业现状分析及对策[J].北方经贸,2015(11):103-105.
[6]陈守江,姜松.果蔬中的酚类化合物及其抗氧化作用(综述)[J].安徽技术师范学院学报,2003,17(2):144-148.
[7]李婧文,周长林.多糖的免疫调节作用及多糖药物的研究进展[J].中国生化药物杂志,2016,36(4):24-28.
[8]管晓冉,张德纯,李金玲,等.双歧杆菌发酵果蔬汁对小鼠免疫功能的影响[J].中国微生态学杂志,2010,22(2):110-113.
[9]尹曼,魏颖,马勇,等.胶原肽和大米肽对黑色素生成的影响[J].食品科技,2017(10):244-247.
[10]魏颖,刘艳,林峰,等.刺玫果免疫调节和体外抗氧化活性研究[J].中国食品学报,2014,14(8):41-46.
[11]魏颖,谷瑞增,林峰,等.乌鸡肽免疫调节作用的研究[J].食品研究与开发,2014(16):1-4.
[12]洪美玲,陈立侨.水产动物养殖胁迫评价及预防[M].北京:中国林业出版社,2012.
[13]于晓艳,任清,卢舒娴,等.微生物发酵液主要功效酶活力的测定[J].食品科技,2008,33(7):193-196.
[14]贾刚,谢丽阳,张迪,等.黄酮对AAPH诱导的Hep G2细胞氧化应激的作用[J].食品科技,2011(9):23-26.
[15]曾丛梅,蔡树涛,周凤兰,等.Con A激活小鼠胸腺T淋巴细胞增殖过程中c-myc与核骨架蛋白的结合[J].中国科学,1996,39(4):511-516.
[16]周灵,彭代智,陈博,等.PHA、Con A及CD3ε单克隆抗体对小鼠淋巴细胞迁移及分泌趋化因子的影响[J].免疫学杂志,2012,28(4):295-299.
[17]HIROTAKA O,SU I H,KENSUKE M,et al.The toll-like receptor protein Rp105 regulates lipopolysaccharide signaling in B cells:[J].Journal of Experimental Medicine,2000,192(1):23.
[18]复方中药对感染NIB鸡淋巴细胞刺激指数的影响[J].山西农业大学学报(自然科学版),2009,29(6):575-578.
[19]MANTOVANI A,SICA A,SOZZANI S,et al.The chemokine system in diverse forms of macrophage activation and polarization[J].Trends in Immunology,2004,25(12):677-686.
[20]PENGAL R A,GANESAN L P,WEI G,et al.Lipopolysaccharide-induced production of interleukin-10 is promoted by the serine/threonine kinase Akt[J].Molecular Immunology,2006,43(10):1557-1564.