空气辅助微生物提高采收率的室内实验研究
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摘要
从三叠系长6油藏产出污泥分离出3株具有较强驱油能力的优势菌株,一株为好氧菌,一株为厌氧菌,一株为兼性厌氧菌。对不同溶氧量下3株菌复配菌液进行了生长规律进行了测定,同时,对空气辅助微生物降解原油能力进行了测定,测试了非均质模型中微生物与空气交替注入驱油效果。通过实验优选出溶氧浓度为4.5 mg/L时,微生物相对较快达到对数生长期。溶氧浓度为4.5~5.5 mg/L时微生物对原油降解率较高。微生物与空气交替注入驱油时,采收率提高17.08%,体现出空气辅助微生物提高采收率优势,对拓宽提高采收率理论及提高矿场增油效果很有意义。
Three fermentative enrichment cultures were obtained from the sludge samples of the Triassic Yanchang 6 reservoir.One strain is aerobic strain,one strain is anaerobic bacteria,and the third one is facultative anaerobes.The growth rhythm of three strains was measured.In addition,the microbial flooding and air-injection displacement in heterogeneous physical model was created.Experiments showed that microorganisms entered the stationary phase relatively quickly at dissolved oxygen concentration of 4.5 mg/L.Physical simulation of oil displacement indicated that oil recovery factor of alternate injection is increased by 17.08%.The synergistic effect of microbial flooding and air-injection displacement is of important practical significance to broaden the improved recovery theory and Improve the effect of field test.
Three fermentative enrichment cultures were obtained from the sludge samples of the Triassic Yanchang 6 reservoir.One strain is aerobic strain,one strain is anaerobic bacteria,and the third one is facultative anaerobes.The growth rhythm of three strains was measured.In addition,the microbial flooding and air-injection displacement in heterogeneous physical model was created.Experiments showed that microorganisms entered the stationary phase relatively quickly at dissolved oxygen concentration of 4.5 mg/L.Physical simulation of oil displacement indicated that oil recovery factor of alternate injection is increased by 17.08%.The synergistic effect of microbial flooding and air-injection displacement is of important practical significance to broaden the improved recovery theory and Improve the effect of field test.
引文
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11 刘涛,曹功泽,巴燕,等.沾3区块内源微生物激活及现场试验.石油与天然气化工,2012;41(4):411—414Liu Tao,Cao Gongze,Ba Yan,et al.Activation of the indigenous microorganism and field test in Zhan 3 block.Chemical Industry of Oil and Gas.2012;41(4):411—414
12 程明明,夏添,雷光伦,等.低渗透油藏多段塞微生物驱开发指标预测.中国石油大学学报(自然科学版),2014;38(6):113 —120Cheng Mingming,Xia Tian,Lei Guanglun,et al.Development index prediction of multi-slug microbial flooding in low permeability reservoir.Journal of China University of Petroleum(Natural Science Edition),2014;38(6):113—120
2 雷光伦,程明明,高雪梅,等.注入参数对微生物提高采收率的影响研究.石油与天然气化工,2014;43(3):287—291Lei Guanglun,Cheng Mingming,Gao Xuemei,et al.Impact of injection parameter on microbial enhanced oil recovery.Chemical Engineering of Oil&Gas,2014;43(3):287—291
3 谢昆,姜康,于登飞,等.微生物油藏反应动力学模型的构建.科学技术与工程,2015;22(2):148—152Xie Kun,Jiang Kang,Yu Dengfei,et al.Development of microorganism reaction kinetic model under reservoir condition.Science Technology and Engineering.2015;22(2):148—152
4 刘建英,程明明,雷光伦,等.安塞特低渗油田微生物驱油室内研究及先导试验.科学技术与工程,2014;14(12):26—30Liu Jianying,Cheng Mingming,Lei Guanglun,et al.Laboratory evaluation and field trial of microbial oil displacement in ansai ultralow permeable oilfield.Science Technology and Engineering,2014;14 (12):26—30
5 Fan Z,She Y H,Li H M,et al.Impact of an indigenous microbial enhanced oil recovery field trial on microbial community structure in a high pour-point oil reservoir.Applied Microbiology and Biotechnology,2012;95(3):811—821
6 包木太,王丽娜,李一鸣,等.一株石油烃降解菌D2-1解烃性能研究.深圳大学学报(理工版),2012;29(6):515—520Bao Mutai,Wang Lina,Li Yiming,et al.Degradation characteristics research of a petroleum-degrading bacteria D2-1.Journal of Shenzhen University Science and Engineering,2012;29(6):515—520
7 王克亮,王继红,李世强,等.定边区块微生物驱油效果研究.科学技术与工程,2011;23(11):5529—5532Wang Keliang,Wang Jihong,Li Shiqiang,et al.Development of microbial enhanced oil recovery in Dingbian block.Science Technology and Engineering,2011;23(11):5529—5532
8 雷光伦,陈月明.微生物采油产量预测模型.石油大学学报(自然科学版),2002;26(1):35—38Lei Guanglun,Chen Yueming.Microbial oil production prediction model.Journal of Petroleum University(Natural Science Edition),2002;26(2):53—56
9 Wang J,Ma T,Zhao L,et al.Monitoring exogenous and indigenous bacteria by PCR-DGGE technology during the process of microbial enhanced oil recovery.Journal of Industrial Microbiology&Biotechnology,2008;35(6):619—628
10 雷光伦,马继业,郭省学,等.微生物菌液物质油水界面扩张黏弹性研究.西安石油大学学报(自然科学版),2008;23(6):74 —77Lei Guanglun,Ma Jiye,Guo Shengxue,et al.Study on the dilation viscoelasticity of the inferface between bacteria liquid and crude oil.Journal of Xi'an Shiyou University(Natural Science Edition),2008;23(6):74—77
11 刘涛,曹功泽,巴燕,等.沾3区块内源微生物激活及现场试验.石油与天然气化工,2012;41(4):411—414Liu Tao,Cao Gongze,Ba Yan,et al.Activation of the indigenous microorganism and field test in Zhan 3 block.Chemical Industry of Oil and Gas.2012;41(4):411—414
12 程明明,夏添,雷光伦,等.低渗透油藏多段塞微生物驱开发指标预测.中国石油大学学报(自然科学版),2014;38(6):113 —120Cheng Mingming,Xia Tian,Lei Guanglun,et al.Development index prediction of multi-slug microbial flooding in low permeability reservoir.Journal of China University of Petroleum(Natural Science Edition),2014;38(6):113—120