帕金森病患者血浆外泌体miR-331-5p表达水平及意义
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摘要
目的探讨帕金森病患者血浆外泌体miR-331-5p表达水平及意义。方法选取2016年8月—2018年7月我院收治的75例帕金森病患者作为研究对象。选取同期体检健康者50例作为对照组。采用RTqPCR法检测二组受试者血浆外泌体miR-331-5p表达水平。比较不同分期帕金森病患者血浆外泌体miR-331-5p表达水平。采用受试者工作特征曲线(ROC)分析血浆外泌体miR-331-5p诊断Ⅱ～Ⅲ期及Ⅳ～Ⅴ期帕金森病的效能。结果帕金森病组患者血浆外泌体miR-331-5p表达水平为(1.03±0.36),明显高于对照组[(0.22±0.06),P<0.05]。不同分期帕金森病患者血浆外泌体miR-331-5p表达水平比较,差异有统计学意义(均P<0.05),其中Ⅴ期患者血浆外泌体miR-331-5p表达水平明显高于Ⅳ、Ⅲ、Ⅱ、Ⅰ期患者(均P<0.05)。血浆外泌体miR-331-5p诊断Ⅱ～Ⅲ期帕金森病的AUC、敏感度、特异度分别为0.863、70.67%、92.00%;血浆外泌体miR-331-5p诊断Ⅳ～Ⅴ期帕金森病的曲线下面积(AUC)、敏感度及特异度分别为0.768、75.00%、74.58%。结论帕金森病患者血浆外泌体miR-331-5p表达水平升高,检测血浆外泌体miR-331-5p表达量有助于评估帕金森病临床分期。
Objective To investigate the expression and significance of plasma exosome miR-331-5p in Parkinson′s disease.Methods Seventy-five patients with Parkinson′s disease admitted to The Central Hospital of Wuhan from August 2016 to July 2018 were selected as the study subjects.Fifty healthy persons in the same period were selected as control group.RT-q PCR was used to detect the expression of miR-331-5p in plasma exosomes of the two groups.The levels of plasma exosome miR-331-5p in patients with Parkinson′s disease at different stages were compared.Subject operating characteristic curve(ROC) was used to analyze the diagnostic efficacy of plasma exosome miR-331-5p in Ⅱ-Ⅲ-stage,Ⅳ-Ⅴ-stage Parkinson′s disease.Results The expression level of plasma exosome miR-331-5p in Parkinson′s group was(1.03 ± 0.36),which was significantly higher than that in the control group(0.22 ± 0.06)(P<0.05).The levels of plasma exosome miR-331-5p in different stages of Parkinson′s disease were significantly different(P<0.05),and the expression level of miR-331-5p in patients with stageVwere significantly higher than that in patients with stage Ⅳ,Ⅲ,Ⅱ and Ⅰ(P<0.05).The AUC,sensitivity and specificity of plasma exosome miR-331-5p in the diagnosis of stage Ⅱ-Ⅲ Parkinson′s were 0.863,70.67% and 92.00%,respectively; the AUC,sensitivity and specificity of plasma exosome miR-331-5p in the diagnosis ofstage Ⅳ-Ⅴ Parkinson′s disease were 0.768,75.00% and 74.58%,respectively.Conclusion The expression level of plasma exosome microRNA-331-5p in patients with Parkinson′s disease is elevated.Detecting the expression level of plasma exosome microRNA-331-5p is helpful to evaluate the clinical stage of Parkinson′s disease.
Objective To investigate the expression and significance of plasma exosome miR-331-5p in Parkinson′s disease.Methods Seventy-five patients with Parkinson′s disease admitted to The Central Hospital of Wuhan from August 2016 to July 2018 were selected as the study subjects.Fifty healthy persons in the same period were selected as control group.RT-q PCR was used to detect the expression of miR-331-5p in plasma exosomes of the two groups.The levels of plasma exosome miR-331-5p in patients with Parkinson′s disease at different stages were compared.Subject operating characteristic curve(ROC) was used to analyze the diagnostic efficacy of plasma exosome miR-331-5p in Ⅱ-Ⅲ-stage,Ⅳ-Ⅴ-stage Parkinson′s disease.Results The expression level of plasma exosome miR-331-5p in Parkinson′s group was(1.03 ± 0.36),which was significantly higher than that in the control group(0.22 ± 0.06)(P<0.05).The levels of plasma exosome miR-331-5p in different stages of Parkinson′s disease were significantly different(P<0.05),and the expression level of miR-331-5p in patients with stageVwere significantly higher than that in patients with stage Ⅳ,Ⅲ,Ⅱ and Ⅰ(P<0.05).The AUC,sensitivity and specificity of plasma exosome miR-331-5p in the diagnosis of stage Ⅱ-Ⅲ Parkinson′s were 0.863,70.67% and 92.00%,respectively; the AUC,sensitivity and specificity of plasma exosome miR-331-5p in the diagnosis ofstage Ⅳ-Ⅴ Parkinson′s disease were 0.768,75.00% and 74.58%,respectively.Conclusion The expression level of plasma exosome microRNA-331-5p in patients with Parkinson′s disease is elevated.Detecting the expression level of plasma exosome microRNA-331-5p is helpful to evaluate the clinical stage of Parkinson′s disease.
引文
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[2] Grimmig B,Daly L,Subbarayan M,et al.Astaxanthin is neuroprotective in an aged mouse model of Parkinson′s disease[J].Oncotarget,2018,9(12):10388-10401
[3] Modo M,Crum W R,Gerwig M,et al.Magnetic resonance imaging and tensor-based morphometry in the MPTP non-human primate model of Parkinson′s disease[J].Plos One,2017,12(7):180733
[4] Yoshimura A,Sawada K,Kimura T.Is the exosome a potential target for cancer immunotherapy?[J].Annals Transl Med,2017,5(5):117-118
[5] Kong F L,Wang X P,Li Y N,et al.The role of exosomes derived from cerebrospinal fluid of spinal cord injury in neuron proliferation in vitro[J].Artif Cells Nanomed Biotechnol,2017,46(1):200-205
[6] Loredana L,Silvia V,L′Episcopo Francesca,et al.microRNAs in Parkinson′s Disease:From Pathogenesis to Novel Diagnostic and Therapeutic Approaches[J].Int J Mol Sci,2017,18(12):2698-2727
[7] Yao YF,Qu MW,Li GC,et al.Circulating exosomal miRNAs as diagnostic biomarkers in Parkinson′s disease[J].Eur Rev Med Pharmacol Sci,2018,22:5278-5283
[8] 中华医学会神经病学分会帕金森病及运动障碍学组,中国医师协会神经内科医师分会帕金森病及运动障碍专业.中国帕金森病的诊断标准(2016版)[J].中华神经科杂志,2016,49(4):268-271
[9] Paolini L,Orizio F,Busatto S,et al.Exosomes secreted by hela cells shuttle on their surface the plasma membrane-associated sialidase NEU3[J].Biochemistry,2017,56(48):6401-6408
[10] Verweij F J,Bebelman M P,Jimenez C R.Quantifying exosome secretion from single cells reveals a modulatory role for GPCR signaling.[J].J Cell Biol,2018,217(3):1129-1142
[11] Cavallini C,Zannini C,Olivi E.Restoring in vivo-like membrane lipidomics promotes exosome trophic behavior from human placental mesenchymal stromal/stem cells[J].Cell Transplant,2018,27(1):55-69
[12] Yao W,Mei C,Nan X,et al.Evaluation and comparison of in vitro degradation kinetics of DNA in serum,urine and saliva:a qualitative study[J].Gene,2016,590(1):142-148
[13] Dean I,Dzinic S H,Bernardo M M,et al.The secretion and biological function of tumor suppressor maspin as an exosome cargo protein[J].Oncotarget,2016,8(5):8043-8056
[14] Bosque A,Dietz L,Gallegolleyda A,et al.Comparative proteomics of exosomes secreted by tumoral Jurkat T cells and normal human T cell blasts unravels a potential tumorigenic role for valosin-containing protein[J].Oncotarget,2016,7(20):29287-29305
[15] Tadayoshi K.MicroRNAs:Potential biomarkers and therapeutic targets for alveolar bone loss in periodontal disease[J].Int J Mol Sci,2016,17(8):1317-1328
[16] Yukawa H,Suzuki K,Aoki K.Imaging of angiogenesis of human umbilical vein endothelial cells by uptake of exosomes secreted from hepatocellular carcinoma cells[J].Sci Rep,2018,8(1):6765-6776
[17] Pasquier C,Gardès,Julien.Prediction of miRNA-disease associations with a vector space model[J].Sci Rep,2016,6(1):27036-27045
[18] Fabre A,Badens C.Human mendelian diseases related to abnormalities of the RNA exosome or its cofactors[J].Intractable Rare Dis Res,2014,3(1):8-11
[19] Margaillan G,Lvesque R,Guillemette C.Epigenetic regulation of steroid inactivating UDP-glucuronosyltransferases by microRNAs in prostate cancer[J].J Steroid Biochem Mol Biolo,2016,155:85-93
[20] Matsumura T,Sugimachi K,Iinuma H,et al.Exosomal microRNA in serum is a novel biomarker of recurrence in human colorectal cancer[J].Br J Cancer,2015,113(2):275-281