广西地方葛根种质资源遗传多样性的SCoT分析
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摘要
为了解广西地方葛根种质资源之间的亲缘关系,本研究以SCoT分子标记对44份广西葛根种质资源的基因组DNA进行PCR扩增,并进行遗传多样性分析。结果表明,25条SCoT引物共扩增出223个条带,其中具有多态性的条带有194条,平均每条引物获得7.76个多态性条带,多态性比例为86.99%。聚类分析表明,44份葛根种质资源的遗传相似系数在0.587～0.982之间。在系数0.65处,44份葛根分为两大类,37号材料单独成为一类;在系数为0.74处,可聚为六类。综上所述,SCoT分子标记适用于葛根种质资源遗传多样性分析,本研究结果为广西葛根种质资源鉴定评价和新品种选育奠定了一定的理论基础。
In order to analyze the genetic similarity of 44 Pueraria germplasm resources in Guangxi, the DNA of the germplasm resources was amplified by SCoT primers.The results showed that, 25 SCoT primers produced 233 bands, among which 194 bands were polymorphic, the percentage of polymorphic rate was up to 86.99%, and the average polymorphic number was 7.76 per primer. The cluster analysis showed that the range of genetic similarity(GS)was 0.587 to 0.982 among 44 Pueraria samples,the samples could be divided into 2 groups at the GS of 0.65, the 37 th germplasm alone was clustered into one group, and the rest accessions was clustered into another group. All of the accessions could be clustered into six groups at the GS of 0.74. The result revealed that SCoT molecular markers are suitable for the analysis of genetic diversity of the Pueraria germplasm resources, and will lay a foundation for the evaluation and selection of the germplasm resources of Pueraria in Guangxi.
In order to analyze the genetic similarity of 44 Pueraria germplasm resources in Guangxi, the DNA of the germplasm resources was amplified by SCoT primers.The results showed that, 25 SCoT primers produced 233 bands, among which 194 bands were polymorphic, the percentage of polymorphic rate was up to 86.99%, and the average polymorphic number was 7.76 per primer. The cluster analysis showed that the range of genetic similarity(GS)was 0.587 to 0.982 among 44 Pueraria samples,the samples could be divided into 2 groups at the GS of 0.65, the 37 th germplasm alone was clustered into one group, and the rest accessions was clustered into another group. All of the accessions could be clustered into six groups at the GS of 0.74. The result revealed that SCoT molecular markers are suitable for the analysis of genetic diversity of the Pueraria germplasm resources, and will lay a foundation for the evaluation and selection of the germplasm resources of Pueraria in Guangxi.
引文
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[21] 查美琴,赵玉玲,李疆,朱瑜,罗淑萍,胡月.新疆枸杞种质资源遗传多样性分析及DNA指纹图谱构建[J].植物遗传资源学报,2016,17(6):1065-1071
[22] 陈虎,何新华,罗聪,朱建华,李峰.龙眼24个品种的SCoT遗传多样性分析[J].园艺学报,2010,37(10):1651-1654
[23] 陈香玲,苏伟强,刘业强,朱建华,李峰.36份菠萝种质的遗传多样性SCoT分析[J].西南农业学报,2012,25(2):625-629
[24] 高岭,冯尚国,何仁锋,赵妙玉,王慧中.兰属植物目标起始密码子(SCoT)遗传多样性分析[J].园艺学报,2013,40(10):2026-2032
[25] 韩国辉,向素琼,汪卫星,贾志刚,洪棋斌,梁国鲁.柑橘SCoT分子标记技术体系的建立及其在遗传分析中的应用[J].园艺学报,2011,38(7):1243-1250
[26] 蒋晓梅,黄琳凯,张新全,张瑜,聂刚,杨盛婷,赵欣欣,严海东.利用SCoT分析柳枝稷遗传资源的多样性[J].植物遗传资源学报,2014,15(1):89-95
[27] 赵瑞强,高燕会,章晓玲,斯金平.铁皮石斛SCoT-PCR反应体系构建及优化[J].核农学报,2012,26(4):648-655
[28] 何仁锋,冯尚国,陈喆,高岭,沈晓霞,沈宇峰,王志安,王慧中.药用菊花SCoT-PCR反应体系的正交优化[J].核农学报,2014,28(10):1781-1789
[29] 李丕睿,蒋甲福,陈素梅,管志勇,廖园,房伟民,陈发棣.菊属植物SCoT分子标记技术在遗传多样性分析中的应用[J].园艺学报,2013,40(10):2015-2025
[30] 王丰丰,王萍,杨静,代彩玲.基于SCoT标记分析籽用南瓜的遗传多样性[J].植物生理学报,2017,53(8):1569-1576
[31] 周慧文,单建伟,冯斗,严华兵.SCoT分子标记技术在木薯遗传多样性分析中的应用[J].热带作物学报,2015,36(8):1440-1444
[32] 陈明辉,张保青,宋修鹏,陈虎,杨丽涛,李杨瑞,陈保善.宿根矮化病菌诱导甘蔗差异表达基因的cDNA-SCoT分析[J].作物学报,2013,39(6):1119-1126
[33] 贺梁琼,熊发前,唐秀梅,蒋菁,韩柱强,钟瑞春,高忠奎,李忠,何新华,唐荣华.花生属人工异源多倍体进化早期基因表达变化的cDNA-SCoT分析[J].作物学报,2014,40(10):1767-1775
[34] 吴建明,李杨瑞,王爱勤,杨柳,杨丽涛.赤霉素诱导甘蔗节间伸长基因的cDNA-SCoT差异表达分析[J].作物学报,2010,36(11):1883-1890
[2] 顾志平,陈碧珠,冯瑞芝,陈四保,仲耘,连文琰.中药葛根及其同属植物的资源利用和评价[J].药学学报,1996,31(5):387-393
[3] 陈元生,柳雪芳.我国葛种质资源的研究与利用[J].长江蔬菜,2008(6):6-9
[4] 时龙飞.广西藤县和平镇葛根之乡[DB/OL].(2017-03-26)[2018-03-01].https://club.1688.com/article/35314966.htm
[5] 杨东.走进“中国粉葛之乡”藤原产业促农民增收[OB/OL].(2012-12-25)[2018-03-01].http://www.gxnews.com.cn/staticpages/20121225/newgx50d988a0-6663330.shtml
[6] 郝建平,王峰,宋强,张静,魏文恺.山西省野葛种质资源分布与植物学性状研究[J].植物遗传资源学报,2016,17(1):39-44
[7] 罗亚红,欧珍贵,周明强,郭春应,李志芳.8个葛根种质资源的性状表现与产量评价[J].贵州农业科学,2015,43(12):158-160
[8] 谭燕群,陈建芳,揭雨成,邢虎成,张英,熊力夫.不同葛种质资源的植物学性状、藤蔓产量和营养品质分析[J].湖南林业科技,2016,43(5):85-87,91
[9] 景戌,徐莉,陈俊意,曾祥琼,黄祖春,朱照静,李化,袁超,王志虹,李士敏,张静文,陈典全.重庆地区葛根遗传多样性分析和葛根素含量聚类分析[J].中国农学通报,2010,26(24):80-82
[10] 魏文恺.山西不同产地野葛的快速繁殖及RAPD分析[D].太原:山西大学,2015:28-32
[11] 周精华,揭雨成,杜晓华,邢虎成,熊力夫.葛种质资源亲缘关系的RAPD分析[J].作物研究,2013,27(4):347-350
[12] 郭艳艳,成春燕,黄静丽,杨旭东,卢家仕,王爱勤,何龙飞,姜伯乐,黄荣韶.不同来源葛根遗传多样性ISSR分析[J].大众科技,2013,15(164):134-136
[13] 袁灿,钟文娟,龚一耘,蒲德强,戢沛城,黄海燕,杨泽湖,张超.葛根资源遗传多样性和性状关联分析[J].植物遗传资源学报,2017,18(2):233-241
[14] 陈大霞,彭锐,李隆云,张雪,王钰.部分粉葛品种遗传关系的SRAP 研究[J].中国中药杂志,2011,36(5):538-541
[15] Collard B C Y,Mackill D J.Start codon targeted (SCoT) polymorphism:A simple,novel DNA marker technique for generating gene-targeted markers in plants[J].Plant Molecular Biology Reporter,2009,27(1):86-93
[16] 熊发前,蒋菁,钟瑞春,韩柱强,贺梁琼,李忠,庄伟建,唐荣华.目标起始密码子多态性(SCoT)分子标记技术在花生属中的应用[J].作物学报,2010,36(12):2055-2061
[17] Chen D X,Zhao J F,Liu X,Wang C H,Zhang Z W,Qin S Y,Zhong G Y.Genetic diversity and geneticstructure of endangered wild Sinopodophyllum emodi by start codon targeted polymorphism[J].China Journal of Chinese Materia Medica,2013,38(2):278-283
[18] Guo D L,Zhang J Y,Liu C H.Genetic diversity in some grape varieties revealed by SCoT analyses[J].Molecular Biology Reports,2012,39(5):5307-5313
[19] Luo C,He X H,Chen H,Schnell R J.Genetic diversity of mango cultivars estimated using SCoT and ISSR markers[J].Biochemical Systematics & Ecologyl,2011,39:676-684
[20] Gorji A M,Poczai P,Polgar Z,Taller J.Efficiency of arbitrarily amplified dominant markers(SCoT,ISSR and RAPD) for diagnostic fingerprinting in tetraploid potato[J].American Journal of Potato Research,2011,88(3):226-237
[21] 查美琴,赵玉玲,李疆,朱瑜,罗淑萍,胡月.新疆枸杞种质资源遗传多样性分析及DNA指纹图谱构建[J].植物遗传资源学报,2016,17(6):1065-1071
[22] 陈虎,何新华,罗聪,朱建华,李峰.龙眼24个品种的SCoT遗传多样性分析[J].园艺学报,2010,37(10):1651-1654
[23] 陈香玲,苏伟强,刘业强,朱建华,李峰.36份菠萝种质的遗传多样性SCoT分析[J].西南农业学报,2012,25(2):625-629
[24] 高岭,冯尚国,何仁锋,赵妙玉,王慧中.兰属植物目标起始密码子(SCoT)遗传多样性分析[J].园艺学报,2013,40(10):2026-2032
[25] 韩国辉,向素琼,汪卫星,贾志刚,洪棋斌,梁国鲁.柑橘SCoT分子标记技术体系的建立及其在遗传分析中的应用[J].园艺学报,2011,38(7):1243-1250
[26] 蒋晓梅,黄琳凯,张新全,张瑜,聂刚,杨盛婷,赵欣欣,严海东.利用SCoT分析柳枝稷遗传资源的多样性[J].植物遗传资源学报,2014,15(1):89-95
[27] 赵瑞强,高燕会,章晓玲,斯金平.铁皮石斛SCoT-PCR反应体系构建及优化[J].核农学报,2012,26(4):648-655
[28] 何仁锋,冯尚国,陈喆,高岭,沈晓霞,沈宇峰,王志安,王慧中.药用菊花SCoT-PCR反应体系的正交优化[J].核农学报,2014,28(10):1781-1789
[29] 李丕睿,蒋甲福,陈素梅,管志勇,廖园,房伟民,陈发棣.菊属植物SCoT分子标记技术在遗传多样性分析中的应用[J].园艺学报,2013,40(10):2015-2025
[30] 王丰丰,王萍,杨静,代彩玲.基于SCoT标记分析籽用南瓜的遗传多样性[J].植物生理学报,2017,53(8):1569-1576
[31] 周慧文,单建伟,冯斗,严华兵.SCoT分子标记技术在木薯遗传多样性分析中的应用[J].热带作物学报,2015,36(8):1440-1444
[32] 陈明辉,张保青,宋修鹏,陈虎,杨丽涛,李杨瑞,陈保善.宿根矮化病菌诱导甘蔗差异表达基因的cDNA-SCoT分析[J].作物学报,2013,39(6):1119-1126
[33] 贺梁琼,熊发前,唐秀梅,蒋菁,韩柱强,钟瑞春,高忠奎,李忠,何新华,唐荣华.花生属人工异源多倍体进化早期基因表达变化的cDNA-SCoT分析[J].作物学报,2014,40(10):1767-1775
[34] 吴建明,李杨瑞,王爱勤,杨柳,杨丽涛.赤霉素诱导甘蔗节间伸长基因的cDNA-SCoT差异表达分析[J].作物学报,2010,36(11):1883-1890