茶树新品系“53-34”父本的EST-SSR标记鉴定
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摘要
本研究采用EST-SSR标记对茶树新品系"53-34"及其母本和4个可能父本进行了分析。结果表明:24对引物共检测到69个等位位点,平均2.88;53-34与湘波绿及4个可能父本的相同位点数分别占其扩增总位点数的85.71%、71.43%、65.71%、60.00%、74.26%,最大为湘波绿,最小为福云7号。6份供试材料间相似系数变幅为0.44~0.77,平均0.57。根据相似系数矩阵按UPGMA法进行聚类,在相似系数平均值0.57处可将参试的6份材料划分为三大类:第Ⅰ类高芽齐;第Ⅱ类湘波绿、53-34与古蔺牛皮茶;第Ⅲ类云大72-04与福云7号。参试材料的相似系数和亲缘关系树状图在分子水平上显示了53-34的4个可能父本中,古蔺牛皮茶与其亲缘关系最近,可能为53-34的父本。
EST-SRR primers were used to identify the male parents for 53-34 tea cultivar among 4 possible male parents. The result showed that totally 69 alleles were amplifi ed using 24 core EST-SSR primers, on average of 2.88.The percentage of common alleles with 53-34 is 85.71%, 71.43%, 65.71%, 60.00%, 74.26%, the maximum is Xiangbolv, the minimum is Fuyun 7. The similarity coeffi cient among different cultivars varied from 0.44-0.77, on average of 0.57. The 6 accessions were classifi ed into 3 groups based on the UPGMA method with the similarity coeffi cient at 0.57, and the group Ⅰ contained Gaoyaqi; the group Ⅱ contained Xiangbolv, 53-34 and Gulin-niupicha; the group Ⅲ contained Yunda 72-04, Fuyun 7. The results of the similarity coeffi cient and dendrogram showed a closer relationship between 53-34 and Gulinniupicha, Gulin-niupicha is the possible male parent of 53-34.
EST-SRR primers were used to identify the male parents for 53-34 tea cultivar among 4 possible male parents. The result showed that totally 69 alleles were amplifi ed using 24 core EST-SSR primers, on average of 2.88.The percentage of common alleles with 53-34 is 85.71%, 71.43%, 65.71%, 60.00%, 74.26%, the maximum is Xiangbolv, the minimum is Fuyun 7. The similarity coeffi cient among different cultivars varied from 0.44-0.77, on average of 0.57. The 6 accessions were classifi ed into 3 groups based on the UPGMA method with the similarity coeffi cient at 0.57, and the group Ⅰ contained Gaoyaqi; the group Ⅱ contained Xiangbolv, 53-34 and Gulin-niupicha; the group Ⅲ contained Yunda 72-04, Fuyun 7. The results of the similarity coeffi cient and dendrogram showed a closer relationship between 53-34 and Gulinniupicha, Gulin-niupicha is the possible male parent of 53-34.
引文
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[10]刘振,赵洋,杨培迪,等.茶树新品种“湘波绿2号”父本的SSR标记鉴定[J].茶叶通讯,2014,41(1):8-14.
[11]Wang D,Liao X L,Cheng L,et al.Development of novel ESTSSR markers in common carp by data mining from public EST sequences[J].Aquaculture,2007,(271):558-574.
[12]Powell W,Machray G.C,Provan J.Polymorphism revealed by simple sequence repeats[J].Trends Plan Sci,1996,(1):215-222.
[13]陈亮,陈大明,高其康,等.茶树基因DNA提取与鉴定[J].茶叶科学,1997,17(2):177-181.
[14]刘振,王新超,赵丽萍,等.基于EST-SSR的西南地区茶树资源遗传多样性和亲缘关系分析[J].分子植物育种,2008,6(1):100-110.
[15]Zhao L P,Liu Z,Chen L,et al.Generation and characterization of polymorphic expressed sequence tag-derived polymorphic microsatellites from tea plant(Camellia sinensis)and crossspecies amplification in its closely related species and varieties[J].Conservation Genetics,2008,(9):1327-1331.
[16]Botstein D,White R L,Skolnick M,et al.Construction of a genetic linkage map in man using restriction fragment length polymorphisms[J].American Journal of Human Genetics,1980,(32):314-331.
[17]Rohlf F J.NTSYSpc:Numerical taxonomy and multivariate analysis system,Version 2.0.New York:Exeter Software,Applied Biostatistics Inc,New York,USA,2000:16-29.
[2]白堃元,虞富莲,杨亚军,等.中国茶树品种志[M].上海:上海科技出版社,2001.
[3]黄映萍.DNA分子标记研究进展[J].中山大学研究生学刊(自然科学、医学版),2010,32(2):27-26.
[4]王春侠,仇敬运.DNA分子标记技术在遗传育种中的应用[J].重庆科技学院学报(自然科学版),2007,9(3):17-19.
[5]田中淳一.Use of RAPD markers for the identifi cation of parentage of tea cultlvars[J].野菜.茶叶试验场研究报告,1996,B(茶叶)9:31-36.
[6]梁月荣,田中淳一.茶树品种资源遗传多态性RAPD分析[J].浙江林学院学报,2000,17(2):215-218.
[7]梁月荣,田中淳一,武田善行,等.应用RAPD分子标记分析“晚绿”品种的杂交亲本[J].茶叶科学,2002,2(1):22-26.
[8]黎星辉,施兆鹏,刘春林,等.云南大叶茶与汝城白毛茶杂交后代的RAPD亲子鉴定[J].茶叶科学,2001,21(2):99-102.
[9]张曙光,董丽娟,杨阳,等.茶树新品种“玉绿”和“玉笋”父本的EST-SSR标记鉴定[J].茶叶科学,2009,29(6):430-435.
[10]刘振,赵洋,杨培迪,等.茶树新品种“湘波绿2号”父本的SSR标记鉴定[J].茶叶通讯,2014,41(1):8-14.
[11]Wang D,Liao X L,Cheng L,et al.Development of novel ESTSSR markers in common carp by data mining from public EST sequences[J].Aquaculture,2007,(271):558-574.
[12]Powell W,Machray G.C,Provan J.Polymorphism revealed by simple sequence repeats[J].Trends Plan Sci,1996,(1):215-222.
[13]陈亮,陈大明,高其康,等.茶树基因DNA提取与鉴定[J].茶叶科学,1997,17(2):177-181.
[14]刘振,王新超,赵丽萍,等.基于EST-SSR的西南地区茶树资源遗传多样性和亲缘关系分析[J].分子植物育种,2008,6(1):100-110.
[15]Zhao L P,Liu Z,Chen L,et al.Generation and characterization of polymorphic expressed sequence tag-derived polymorphic microsatellites from tea plant(Camellia sinensis)and crossspecies amplification in its closely related species and varieties[J].Conservation Genetics,2008,(9):1327-1331.
[16]Botstein D,White R L,Skolnick M,et al.Construction of a genetic linkage map in man using restriction fragment length polymorphisms[J].American Journal of Human Genetics,1980,(32):314-331.
[17]Rohlf F J.NTSYSpc:Numerical taxonomy and multivariate analysis system,Version 2.0.New York:Exeter Software,Applied Biostatistics Inc,New York,USA,2000:16-29.