血清铁和铁蛋白水平在海人酸诱导的大鼠癫痫模型中的意义
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摘要
目的探讨海人酸(KA)诱导的癫痫大鼠血液中铁和铁蛋白水平的变化。方法 30只SD大鼠通过随机数字表法分为模型组和对照组(每组各15只),模型组采用一次性腹腔注射KA的方式建立癫痫大鼠模型,对照组采用腹腔注射等量生理盐水,分别于给药前1周和给药后2个月取静脉血,应用原子吸收光谱仪来测定血清铁,采用放射性标记酶联免疫分析法测定血清铁蛋白,分别观察两组大鼠血清铁和血清铁蛋白的变化。结果模型组血清铁、血清铁蛋白含量分别为(3.502±0.475) mg/L、(29.229±1.912)μg/L,均明显高于对照组的(2.408±0.760) mg/L、(23.449±1.711)μg/L(t=1.328、19.169,P=0.018 3、0.026 7)。血清铁、血清铁蛋白与癫痫发作具有较强相关性,相关系数分别为0.633、0.732(P值分别为0.035 0、0.013 9)。结论 KA诱导的大鼠癫痫模型血液中铁和铁蛋白水平升高,可能与海人酸诱导的癫痫发作有关。
Objective To explore the changes of serum iron and ferritin levels in kainic acid(KA)-induced epilepsy rats.Methods Thirty SD rats were randomized into model group(n=15) and control group(n=15) according to the random number table.Model group received an intraperitoneal injection of KA to establish the model of epilepsy,while control group was injected with normal saline.Venous blood was collected one week before the medicine was given and 2 months after.Serum iron was determined by atomic absorption spectrometer and serum ferritin was determined by radiolabeled enzyme linked immunosorbent assay(ELISA) in two groups of rats and the changes were observed respectively.Results The levels of serum iron and serum ferritin were(3.502±0.475) mg/L and(29.229±1.912) μg/L,respectively,which were significantly higher than the control group [(2.408±0.760) mg/L and(23.449±1.711) μg/L,respectively;t=1.328,19.169;P=0.018 3,0.026 7].There was a close correlation between serum iron,serum ferritin and the onset of seizures,and the correlation coefficients were 0.633 and 0.732(the P values were 0.035 0,0.013 9).Conclusions The increase of serum iron and ferritin may be related to kainic acid induced epilepsy.
Objective To explore the changes of serum iron and ferritin levels in kainic acid(KA)-induced epilepsy rats.Methods Thirty SD rats were randomized into model group(n=15) and control group(n=15) according to the random number table.Model group received an intraperitoneal injection of KA to establish the model of epilepsy,while control group was injected with normal saline.Venous blood was collected one week before the medicine was given and 2 months after.Serum iron was determined by atomic absorption spectrometer and serum ferritin was determined by radiolabeled enzyme linked immunosorbent assay(ELISA) in two groups of rats and the changes were observed respectively.Results The levels of serum iron and serum ferritin were(3.502±0.475) mg/L and(29.229±1.912) μg/L,respectively,which were significantly higher than the control group [(2.408±0.760) mg/L and(23.449±1.711) μg/L,respectively;t=1.328,19.169;P=0.018 3,0.026 7].There was a close correlation between serum iron,serum ferritin and the onset of seizures,and the correlation coefficients were 0.633 and 0.732(the P values were 0.035 0,0.013 9).Conclusions The increase of serum iron and ferritin may be related to kainic acid induced epilepsy.
引文
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[2] EID T,GRUENBAUM SE,DHAHER R,et al.The glutamate-glutamine cycle in epilepsy[J].Adv Neurobiol,2016,13:351-400.
[3] 云德波,杨宇焦,张逵,等.瘤周谷氨酸、天门冬氨酸水平与胶质瘤继发性癫痫的相关性[J].中国临床神经外科杂志,2016,21(6):331-332,335.
[4] 王静,王法祥,杨云鹏,等.Snail在海人酸诱导癫痫持续状态小鼠皮层中高表达[J].细胞与分子免疫学杂志,2016,32(9):1193-1196.
[5] 王潇慧,鄢泽然,张青,等.柴贝止痫汤调节海人酸致痫大鼠脑组织耐药蛋白MRP2表达的研究[J].中华中医药杂志,2016,31(12):4961-4965.
[6] 高芳,高向东,李翔.婴幼儿缺铁性贫血与其智能发育的关系分析[J].临床医药实践,2016,25(12):909-911.
[7] ACOSTA-CABRONERO J,CARDENAS-BLANCO A,BETTS MJ,et al.The whole-brain pattern of magnetic susceptibility perturbations in Parkinson's disease[J].Brain,2017,140(1):118-131.
[8] 吴靖,周宏斌,潘松青.莲心碱对氯化锂—匹鲁卡品致癫模型急性期皮层脑电图的影响[J].安徽医药,2016,20(3):449-453.
[9] ZHANG Z,LIAO W,BERNHARDT B,et al.Brain iron redistribution in mesial temporal lobe epilepsy:a susceptibility-weighted magnetic resonance imaging study[J].BMC Neurosci,2014,15:117.
[10] 李欣,罗海龙,陈雪寒,等.海人酸癫痫大鼠海马区能量代谢异常和线粒体功能障碍的变化[J].中国老年学杂志,2013,33(11):2595-2596.
[11] JO A,HEO C,SCHWARTZ TH,et al.Nanoscale intracortical iron injection induces chronic epilepsy in rodent[J].J Neurosci Res,2014,92(3):389-397.
[12] DAS J,SINGH R,SHARMA D.Antiepileptic effect of fisetin in iron-induced experimental model of traumatic epilepsy in rats in the light of electrophysiological,biochemical,and behavioral observations[J].Nutr Neurosci,2017,20(4):255-264.
[13] 罗赟飞,吴迪炯,温晓文,等.姜黄素在铁过载疾病治疗中的研究进展[J].浙江临床医学,2014,16(5):822-823.
[14] JYOTI A,SETHI P,SHARMA D.Curcumin protects against electrobehavioral progression of seizures in the iron-induced experimental model of epileptogenesis[J].Epilepsy Behav,2009,14(2):300-308.
[15] 徐其岭,闫莉,谭毅.外伤性癫痫发病机制的研究进展[J].中国临床新医学,2013,6(9):913-916.
[16] FRIEDMAN A,KAUFER D,HEINEMANN U.Blood-brain barrier breakdown-inducing astrocytic transformation:Novel targets for the prevention of epilepsy[J].Epilepsy Research,2009,85(2):142-149.
[17] MICHALAK Z,SANO T,ENGEL T,et al.Spatio-temporally restricted blood-brain barrier disruption after intra-amygdala kainic acid-induced status epilepticus in mice[J].Epilepsy Res,2013,103(2/3):167-179.