剩余污泥中微生物絮凝剂的提取方法比较
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摘要
分别采用低浓度和高浓度剩余污泥对剩余污泥中微生物絮凝剂(MBF)的多种提取方法(超声法、树脂法、超声-树脂法、树脂-超声法、超声-树脂-超声法和树脂-超声-树脂法等)进行了比较研究。结果表明,各种复合形式的提取效果均优于超声法或树脂法单独使用的提取效果。在各种复合形式中,以树脂-超声法所提取MBF的含量最高且絮凝效果最好。此外,所提取的MBF浓度与污泥浓度正相关。从剩余污泥中直接提取MBF,在降低MBF生产成本的同时实现了污泥的资源化利用。
With excess sludge samples of both low concentration and high concentration,microbial flocculant(MBF) extraction was investigated by various extraction methods,including ultrasonic method,cation resin method,ultrasonic-cation resin method,cation resin-ultrasonic method,ultrasonic-cation resin-ultrasonic method,and cation resin-ultrasonic-cation resin method.The experimental results showed that MBF extraction by each compound method was better than that by ultrasonic method or cation resin method alone.Among the compound methods,cation resin-ultrasonic method was the best one,which obtained the highest MBF concentration as well as the best flocculating efficiency.In addition,the extracted MBF concentration was positively correlated with the concentration of the excess sludge.MBF extraction from excess sludge would not only save the cost of MBF production,but also realize the resource reuse of excess sludge.
With excess sludge samples of both low concentration and high concentration,microbial flocculant(MBF) extraction was investigated by various extraction methods,including ultrasonic method,cation resin method,ultrasonic-cation resin method,cation resin-ultrasonic method,ultrasonic-cation resin-ultrasonic method,and cation resin-ultrasonic-cation resin method.The experimental results showed that MBF extraction by each compound method was better than that by ultrasonic method or cation resin method alone.Among the compound methods,cation resin-ultrasonic method was the best one,which obtained the highest MBF concentration as well as the best flocculating efficiency.In addition,the extracted MBF concentration was positively correlated with the concentration of the excess sludge.MBF extraction from excess sludge would not only save the cost of MBF production,but also realize the resource reuse of excess sludge.
引文
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[15]张志强,李向蓉,张艳君,等.一种利用剩余污泥制备生物絮凝剂的方法:中国专利,102336459,2012-02-01
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[19]Christensen J.R.,Srensen P.B.,Christensen G.L.,etal.Mechanisms for overdosing in sludge conditioning.Journal of Environmental Engineering,1993,119(1):159-171
[2]Bala S.S.,Yan S.,Tyagi R.D.,et al.Extracellularpolymeric substances(EPS)producing bacterial strains ofmunicipal wastewater sludge:Isolation,molecular identifi-cation,EPS characterization and performance for sludgesettling and dewatering.Water Research,2010,44(7):2253-2266
[3]Liu W.J.,Yuan H.L.,Yang J.S.,et al.Characterizationof bioflocculants from biologically aerated filter backwashedsludge and its application in dying wastewater treatment.Bioresource Technology,2009,100(9):2629-2632
[4]Wang S.G.,Gong W.X.,Liu X.W.,et al.Productionof a novel bioflocculant by culture of Klebsiella mobilis u-sing dairy wastewater.Biochemical Engineering Journal,2007,36(2):81-86
[5]Kurane R.,Hatamochi K.,Kakuno T.,et al.Productionof a bioflocculant by Rhdococcus erythropolis S-1 grow on al-cohol.Bioscience Biotechnology and Biochemistry,1994,58(2):428-429
[6]Fujita M.,Citrobacterlke M.,Tachibana S.,et al.Char-acterization of a bioflocculant produced by sp:TKF04 fromacetic and propionic acids.Journal of Bioscience and Bio-engineering,2000,89(1):40-46
[7]李大鹏,马放,侯宁,等.味精废水资源化制备复合型生物絮凝剂.湖南大学学报(自然科学版),2009,36(9):78-82Li Dapeng,Ma Fang,Hou Ning,et al.Preparation ofcompound bio-flocculants by using waste zymotic fluid ofglutamic acid fermentation as substrate.Journal of HunanUniversity(Natural Sciences),2009,36(9):78-82(inChinese)
[8]Zhang Z.Q.,Lin B.,Xia S.Q.,et al.Production andapplication of a novel bioflocculant by multiple-microorgan-ism consortia using brewery wastewater as carbon source.Journal of Environmental Sciences,2007,19(6):667-673
[9]高廷耀,顾国维,周琪.水污染控制工程(第3版)(下册).北京:高等教育出版社,2007
[10]国家环境保护总局.水和废水监测分析方法(第4版).北京:中国环境科学出版社,2002
[11]Adav S.S.,Lee D.J.Extraction of extracellular poly-meric substances from aerobic granule with compact interi-or structure.Journal of Hazardous Materials,2008,154(1-3):1120-1126
[12]Bradford M.M.A rapid and sensitive method for thequantitation of microgram quantities of protein utilizing theprinciple of proteindye binding.Analytical Biochemistry,1976,72(1-2):248-254
[13]Chaplin M.F.,Kennedy J.F.Carbohydrate analysis(2nd edition).New York:Oxford University Press,1994
[14]王春燕,周集体,金若菲.利用脱水污泥制备微生物絮凝剂及其絮凝条件研究.中国给水排水,2007,23(21):105-108Wang Chunyan,Zhou Jiti,Jin Ruofei.Preparation andflocculation properties of bioflocculant from dewateredsludge.China Water&Wastewater,2007,23(21):105-108(in Chinese)
[15]张志强,李向蓉,张艳君,等.一种利用剩余污泥制备生物絮凝剂的方法:中国专利,102336459,2012-02-01
[16]Nomura T.,Araki S.,Nagao T.,et al.Resource recov-ery treatment of waste sludge using a solubilizing reagent.Journal of Material Cycles and Waste Management,2007,9(1):34-39
[17]龙腾锐,罗太忠,龙向宇,等.超声波-树脂法提取活性污泥胞外聚合物的研究.给水排水,2008,34(5):34-39Long Tengrui,Luo Taizhong,Long Xiangyu,et al.Re-search on extraction of extracellular polymeric substancesfrom activated sludge by ultrasonic and cation resin.Wa-ter&Wastewater Engineering,2008,34(5):34-39(inChinese)
[18]Park C.,Novak J.T.Characterization of activated sludgeexocellular polymers using several cation-associated ex-traction methods.Water Research,2007,41(8):1679-1688
[19]Christensen J.R.,Srensen P.B.,Christensen G.L.,etal.Mechanisms for overdosing in sludge conditioning.Journal of Environmental Engineering,1993,119(1):159-171