山东地区新型鸭呼肠孤病毒致病特点与变异分析
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摘要
本研究将2011~2018年从山东地区分离到的8株新型鸭呼肠孤病毒进行了毒力和致病性试验,结果显示,8株毒株对鸭胚、鸭胚成纤维细胞、雏鸭的致病性基本一致,鸭胚毒价为10-5.00~10-6.00/0.1 m L,鸭胚成纤维细胞的毒价为10-5.33~10-6.30/0.1 m L;对8株毒株的主要抗原σC蛋白核苷酸和氨基酸序列进行比对分析,结果显示,核苷酸同源性为94.5%~99.6%,氨基酸同源性为95.0%~99.4%;通过绘制遗传进化树发现,与全国其他地区毒株相比,山东地区分离的这8株分离株处于一个独立分支,并且这8株分离株的遗传进化与时间有着密切的相关性,暗示山东地区新型鸭呼肠孤病毒随着时间的推移在不断发生着变异。
In this study, the virulence and pathogenicity tests of 8 novel duck reovirus isolate strains from Shandong area in 2011-2018 were carried out. It was found that the pathogenicities of these above strains in duck embryo, duck embryo fibroblast and duckling were basically consistent. The titer in duck embryo were 10-5.00~10-6.00/0.1 m L, and the titers in duck embryo fibroblast were 10-5.33 ~ 10-6.30/0.1 m L. The nucleotide and amino acid sequences of the major antigen σC protein of these strains were compared and analyzed, conclusively found that the homologies of their nucleotides were 94.5% ~ 99.6%, the homologies of amino acid were 95% ~ 99.4%. By mapping the phylogenetic tree, it is found that Shandong isolates are in a separate branch compared with other strains in other parts of the country. The conclusion is as follows the genetic evolution of σC proteins has one close correlation with their isolating time, so novel duck reovirus in Shandong area is mutating over time.
In this study, the virulence and pathogenicity tests of 8 novel duck reovirus isolate strains from Shandong area in 2011-2018 were carried out. It was found that the pathogenicities of these above strains in duck embryo, duck embryo fibroblast and duckling were basically consistent. The titer in duck embryo were 10-5.00~10-6.00/0.1 m L, and the titers in duck embryo fibroblast were 10-5.33 ~ 10-6.30/0.1 m L. The nucleotide and amino acid sequences of the major antigen σC protein of these strains were compared and analyzed, conclusively found that the homologies of their nucleotides were 94.5% ~ 99.6%, the homologies of amino acid were 95% ~ 99.4%. By mapping the phylogenetic tree, it is found that Shandong isolates are in a separate branch compared with other strains in other parts of the country. The conclusion is as follows the genetic evolution of σC proteins has one close correlation with their isolating time, so novel duck reovirus in Shandong area is mutating over time.
引文
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[10]韩宏宇,马秀丽,黄兵,等.用荧光定量RT-PCR方法检测新型鸭呼肠孤病毒[J].浙江农业学报,2015,27(8):1331-1336.
[2]黄瑜,苏敬良,施少华,等.我国鸭呼肠孤病毒感染相关的疫病[J].中国兽医杂志,2009,45(7):57-58.
[3]王劭,陈少莺,陈仕龙,等.新型鸭呼肠孤病毒NP03株S3基因序列分析[J].农业生物技术学报,2010,18(3):567-572.
[4]陈少莺,陈仕龙,林锋强,等.一种新的鸭病(暂名鸭出血性坏死性肝炎)病原学研究初报[J].中国农学通报,2009,25(16):28-31.
[5]黄瑜,傅光华,施少华,等.新致病型鸭呼肠孤病毒的分离鉴定[J].中国兽医杂志,2009,45(12):29-31.
[6]陈宗艳,朱英奇,王世传,等.一株新型鸭源呼肠孤病毒(TH11株)的分离与鉴定[J].中国动物传染病学报,2012,20(1):10-15.
[7]梁国智,卿柯香,梅敏敏,等.新型鸭呼肠孤病毒DRVXX株全基因组序列测定及分析[J].中国预防兽医学报,2017,39(1):24-27.
[8]张大丙.水禽新现疾病的发生概况和防制思路[J].中国兽药杂志,2009,43(10):42-45.
[9]林锋强,程晓霞,陈仕龙,等.新型鸭呼肠孤病毒在番鸭体内分布和排毒规律[J].中国家禽,2018,40(3):53-55.
[10]韩宏宇,马秀丽,黄兵,等.用荧光定量RT-PCR方法检测新型鸭呼肠孤病毒[J].浙江农业学报,2015,27(8):1331-1336.