羊肚菌多糖提取及其抗氧化活性研究
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摘要
为了探讨碱法提取羊肚菌多糖的工艺条件并测定其抗氧化活性,该研究以四川北川羊肚菌为原料,采用碱法提取羊肚菌多糖,利用苯酚-硫酸法对羊肚菌多糖得率进行测定,并通过单因素探讨提取温度(70、80、90、100℃)、提取时间(2、4、6、8 h)、碱液浓度(0.4、0.6、0.8、1.0 mol·L~(-1))、料液比(1∶15、1∶20、1∶25、1∶30 g·mL~(-1))对羊肚菌多糖得率的影响,同时采用正交试验优化提取工艺,对其抗氧化活性进行测定。结果表明:在提取温度90℃、提取时间5 h、碱液浓度0.7 mol·L~(-1)、料液比1∶20(g·mL~(-1))条件下得到的羊肚菌多糖得率为5.39%。羊肚菌多糖具有较强的清除DPPH自由基、羟自由基、超氧阴离子的能力和较好的还原能力,其IC50分别为0.468、0.208、0.022、0.014 mg·mL~(-1),抗氧化能力依次为还原能力>超氧阴离子清除能力>羟自由基清除能力>DPPH自由基清除能力。优化后的羊肚菌多糖提取工艺合理、可行,且羊肚菌多糖具有较强的抗氧化活性。
The alkali extraction method of polysaccharide from Morchella was optimized and its antioxidant activity was studied. Morchella in Beichuan County of Sichuan Province was selected as raw material,adopting alkali extraction method to extract polysaccharide,and then the polysaccharide extraction content was determined by phenol-sulfuric acid method. The extraction process of the polysaccharide was studied by single factor experiment and orthogonal experiment,which controlled extraction temperature( 70,80,90,100 ℃),extraction time( 2,4,6,8 h),alkali concentration( 0.4,0.6,0.8,0.6 mol·L~(-1)),solid-liquid ratio( 1 ∶ 15,1 ∶ 20,1,25 ∶ 30 g·m L~(-1)). Those antioxidant activities of polysaccharide were also assayed. The results of the optimal extraction conditions were as follows: Extraction temperature was 90 ℃,extraction time 5 h,concentration of alkali concentration 0.7 mol·L~(-1) and solid-liquid ratio 1 ∶ 20 g·m L~(-1),the maximum yield of polysaccharide was 5.39%. The polysaccharide of Morchella had a powerful scavenge DPPH free radicals,hydroxyl radicals,superoxide anions,as well as the good reduction capacity,whose IC50 were 0.468,0.208,0.022,0.014 mg·m L~(-1),respectively. The polysaccharide of Morchella antioxidant capacity was arranged successively reduction capacity > superoxide anion > scavenging capacity > DPPH free radical scavenging capacity. The optimized extraction process of polysaccharide from Morchella was reasonable,feasible and had strong antioxidant activity.
The alkali extraction method of polysaccharide from Morchella was optimized and its antioxidant activity was studied. Morchella in Beichuan County of Sichuan Province was selected as raw material,adopting alkali extraction method to extract polysaccharide,and then the polysaccharide extraction content was determined by phenol-sulfuric acid method. The extraction process of the polysaccharide was studied by single factor experiment and orthogonal experiment,which controlled extraction temperature( 70,80,90,100 ℃),extraction time( 2,4,6,8 h),alkali concentration( 0.4,0.6,0.8,0.6 mol·L~(-1)),solid-liquid ratio( 1 ∶ 15,1 ∶ 20,1,25 ∶ 30 g·m L~(-1)). Those antioxidant activities of polysaccharide were also assayed. The results of the optimal extraction conditions were as follows: Extraction temperature was 90 ℃,extraction time 5 h,concentration of alkali concentration 0.7 mol·L~(-1) and solid-liquid ratio 1 ∶ 20 g·m L~(-1),the maximum yield of polysaccharide was 5.39%. The polysaccharide of Morchella had a powerful scavenge DPPH free radicals,hydroxyl radicals,superoxide anions,as well as the good reduction capacity,whose IC50 were 0.468,0.208,0.022,0.014 mg·m L~(-1),respectively. The polysaccharide of Morchella antioxidant capacity was arranged successively reduction capacity > superoxide anion > scavenging capacity > DPPH free radical scavenging capacity. The optimized extraction process of polysaccharide from Morchella was reasonable,feasible and had strong antioxidant activity.
引文
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LIU MM,SUN LP,ZHUANG YL,et al.,2013.Analysis of polysaccharide composition and in vitro antioxidant activities of fruiting bodies of Catathelasma ventricosum[J].Sci Technol Food Ind,34(21):72-77.[刘蒙蒙,孙丽平,庄永亮,等,2013.梭柄松孢菇子实体中多糖组分及体外抗氧化活性分析[J].食品工业科技,34(21):72-77.]
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LI FL,ZHAO LD,GAO Y,et al.,2011.Comparison between antioxidant activities of polysaccharides extracts from two kinds of edible mushroom[J].Hunan Agric Sci,(9):108-111.[李芳亮,赵立冬,高杨,等,2011.两种食用菌多糖提取物的抗氧化活性比较研究[J].湖南农业科学,(9):108-111.]
LIU JC,LIU XF,ZHANG X,et al.,2018.Optimization of the hot eater extraction technology of polysaccharide from nostoc commune[J].Mol Plant Breed,16(13):4425-4430.[刘继超,刘晓风,张璇,等,2018.地木耳多糖热水提取工艺优化[J].分子植物育种,16(13):4425-4430.]
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