电针心经心包经穴对大脑中动脉梗阻模型大鼠心肌酶谱的影响
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摘要
目的:观察电针心经心包经穴对大脑中动脉梗阻(MCAO)模型大鼠脑缺血损伤后心肌酶谱的影响。方法:将120只SD大鼠随机分为6组,每组分设24、72 h两个亚组,每组10只。经穴组予以电针相应穴位,每12 h 1次,模型组、正常组捆绑处理,各组电针及捆绑处理大鼠后,颈动脉取血,采用双抗夹心酶联免疫法检测CK、CK-MB、LDH、α-HBDH的变化。结果:各组MCAO大鼠造模后血清CK、CK-MB、LDH、α-HBDH明显增高;电针内关组能明显下调CK、CK-MB、α-HBDH的升高,并优于照海穴、人中穴。电针内关、人中能抑制LDH升高,照海组能抑制α-HBDH表达。结论:电针内关、神门穴(尤其是内关)能抑制CK、CK-MB、α-HBDH在心肌中的过度释放,达到减轻心肌组织损伤,保护心脏作用,且作用强于人中、照海穴。
Objective:To observe the effects of different acupoints on myocardial enzyme in MCAO model rats. Methods: Totally 120 SD rats were randomly divided into 6 groups, with 24 hand 72 h subgroups in each group, 10 for each group. The changes of CK, CK-MB, LDH and alpha-HBDH were detected by ELISA after treatment of rats. Results:(1)The serum levels of CK, ck-mb, LDH and alpha-HBDH of the MCAO rats in each group were significantly higher.(2)The Neiguan(PC6)control group can significantly lower the rise of CK, ck-mb and alpha-HBDHand better than electroacupuntrue at Zhaohai(K16) and Renzhong(DU26). Electroacupuncture can inhibit the elevation of LDH and the expression of alpha-HBDH can be suppressed by electroacupunture at Zhaohai(K16). Conclusion:The electroacupuncture at Neiguan(PC6) and Shenmen(HT7) can inhibit the release of CK, CK-MB and alpha-HBDH in myocardial tissue to reduce the damage, protect the heart function, and the effect is stronger than electroacupunture at Zhaohai(K16) and Renzhong(DU26).
Objective:To observe the effects of different acupoints on myocardial enzyme in MCAO model rats. Methods: Totally 120 SD rats were randomly divided into 6 groups, with 24 hand 72 h subgroups in each group, 10 for each group. The changes of CK, CK-MB, LDH and alpha-HBDH were detected by ELISA after treatment of rats. Results:(1)The serum levels of CK, ck-mb, LDH and alpha-HBDH of the MCAO rats in each group were significantly higher.(2)The Neiguan(PC6)control group can significantly lower the rise of CK, ck-mb and alpha-HBDHand better than electroacupuntrue at Zhaohai(K16) and Renzhong(DU26). Electroacupuncture can inhibit the elevation of LDH and the expression of alpha-HBDH can be suppressed by electroacupunture at Zhaohai(K16). Conclusion:The electroacupuncture at Neiguan(PC6) and Shenmen(HT7) can inhibit the release of CK, CK-MB and alpha-HBDH in myocardial tissue to reduce the damage, protect the heart function, and the effect is stronger than electroacupunture at Zhaohai(K16) and Renzhong(DU26).
引文
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[2] 赵步长,伍海勤,赵涛,等.中医脑心同治论[M].北京:人民卫生出版社,2010:14-21.
[3] 刘卫红,周明学,李思耐,等. 脑心同治理论对临床的指导意义[J]. 世界中医药,2017,12(2):241-242,246
[4] 赵涛,赵步长,贾力夫,等. “脑心同治”理论研究进展[J]. 中医临床研究,2015,7(27) :8-10,13.
[5] 章薇,严洁.试论中医心脑与“神明”[J].湖南中医药导报,2003,1:11-13.
[6] 章薇.试论心神、脑神、五脏神及其关系[J].湖南中医学院学报,2003,3:28-29,44.
[7] Zea Longa EL, Weinstein PR, Carlson S, et al. Reversible middle cerebral artery: occlusion without craniectomy in rats[J].Stroke,1989,20:84-91.
[8] Koizumi J,Yoshidy,Nakazawa t,et al.Experimental studies of ischemia brina edema,A new Experimental model of cerebral embolism in rats in which recirulation can be introduced in the ischemia area[J]. stroke,1986,8:1.
[9] 潘江,章薇,葛卫军,等. 改良永久性线栓法制作大鼠大脑中动脉梗阻模型的探讨[J]. 湖南中医药大学学报,2011(3):24-26,44.
[11] 孙运娟.脑缺血后神经元凋亡基因凋亡研究进展[J].医学综述,1999,5(11):481-482.
[12] 李艳茹.“脑心同治”理论研究发展概况[J].世界中西医结合杂志,2011,11(1): 58-59.
[13] 吴玉彬,刘玉灵.心脑同治冠心病心绞痛26例[J].中国临床药理学与治疗学,2001,6(1): 75.
[14] 曹健,周美启,吴生兵.针刺对脑心综合征大鼠颈交感神经节去甲肾上腺素转运蛋白mRNA和心肌β1肾上腺素能受体mRNA表达的影响[J].针刺研究,2011,36(4):252-257.
[15] 林萍.脑出血患者并脑-心综合征的发病及心肌酶谱变化情况[J].中国民康医学,2007, 19(5):372.
[16] 俸军林.急性脑血管病患者心肌酶谱改变及其临床意义[J].临床荟萃,2003, 18(13):723-724.
[17] 王颖,李迪,戴俭宇,等.电针内关穴及列缺穴对心肌缺血大鼠心肌细胞蛋白激酶表达的影响[J].中国中西医结合杂志,2015,35(3):338-342.
[18] 周文娟,章薇,潘江,等. 电针心经穴、心包经穴对脑心综合征大鼠血清儿茶酚氨的影响[J]. 辽宁中医杂志,2016,43(1):177-179.
[19] 李迪,王颖,戴俭宇,等.电针内关穴对心肌缺血大鼠ATP敏感性钾离子通道蛋白的影响[J].中国中医基础医学杂志,2015,21(5):571-574.
[20] 傅立新,赵建国,赵成彬.针刺对实验性脑出血大鼠不同脑区及心肌内皮素mRNA表达的影响[J].中国针灸,2006,26(3):219-223.