三种常见枸杞子中多糖提取方法研究进展
|
摘要
系统综述3种常见枸杞,即新疆枸杞、黑果枸杞和宁夏枸杞中枸杞多糖提取方法,包括浸提法、超声提取法、酶提取法,微波提取法4种常规方法及高压脉冲电场提取法,高速剪切技术辅助提取法,超声辅助亚临界水提取法3种新方法。重点归纳、比较并分析了各种方法的工作原理、优缺点和提取工艺参数,以期为不同枸杞子多糖的充分利用提供参考。
The extraction methods of polysaccharides from three varieties of Chinese wolfberry(L.dasystemum Pojark.,Lycium ruthenicum Murr. and Lycium barbarum L.)were systematically summarized.The methods include four traditional methods(soaking extracting method,ultrasonic assisted extraction,enzyme assisted extraction and microwave assisted extraction method)and three new methods(high intensity pulsed electric field,high-speed shear dispersing emulsifier and ultrasonic assisted subcritical water extraction).The working principles,advantages,disadvantages and technological parameter of various methods were mainly summarized,compared and analysed to provide reference for the full utilization of polysaccharides from varieties of Chinese wolfberry.
The extraction methods of polysaccharides from three varieties of Chinese wolfberry(L.dasystemum Pojark.,Lycium ruthenicum Murr. and Lycium barbarum L.)were systematically summarized.The methods include four traditional methods(soaking extracting method,ultrasonic assisted extraction,enzyme assisted extraction and microwave assisted extraction method)and three new methods(high intensity pulsed electric field,high-speed shear dispersing emulsifier and ultrasonic assisted subcritical water extraction).The working principles,advantages,disadvantages and technological parameter of various methods were mainly summarized,compared and analysed to provide reference for the full utilization of polysaccharides from varieties of Chinese wolfberry.
引文
[1]徐常青,刘赛,徐荣,等.我国枸杞主产区生产现状调研及建议[J].中国中药杂志,2014,39(11):1979-1984
[2]AMAGASE H,FARNSWORTH N R.A review of botanical characteristics,phytochemistry,clinical relevance in efficacy and safety of Lycium barbarum fruit(Goji)[J].Food Research International,2011,44(7):1702-1717
[3]JIN M L,HUANG Q S,ZHAO K,et al.Biological activities and potential health benefit effects of polysaccharides isolated from Lycium barbarum L.[J].International Journal of Biological Macromolecules,2013,54(1):16-23
[4]XIA G Q,XIN N,LIU W,et al.Inhibitory effect of Lycium barbarum polysaccharides on cell apoptosis and senescence is potentially mediated by the p53 signaling pathway[J].Molecular Medicine Reports,2014,9(4):1237-1241
[5]LI J,WANG L,LI J,et al.Lycium barbarum polysaccharide attenuates high-fat diet-induced hepatic steatosis by up-regulating SIRT1expression and deacetylase activity[J].Scientific Reports,2016,6:36209
[6]LIU S Y,CHEN L,LI X C,et al.Lycium barbarum polysaccharide protects diabetic peripheral neuropathy by enhancing autophagy via mTOR/p70S6K inhibition in Streptozotocin-induced diabetic rats[J].Journal of Chemical Neuroanatomy,2017,89:37-42
[7]Wu H T,He X J,Hong Y K,et al.Chemical characterization of Lycium barbarum polysaccharides and its inhibition against liver oxidative injury of high-fat mice[J].International Journal of Biological Macromolecules,2010,46(5):540-543
[8]XIAO J,LIONG E C,CHING Y P,et al.Lycium barbarum polysaccharides protect mice liver from carbon tetrachloride-inducedoxidative stress andnecroinflammation[J].Journal of Ethnopharmacology,2012,139(2):462-470
[9]KE M,ZHANG X J,HAN Z H,et al.Extraction,purification of Lycium barbarum polysaccharides and bioactivity of purified fraction[J].Carbohydrate Polymers,2011,86(1):136-141
[10]ZHAO R,JIN R,CHEN Y,et al.Hypoglycemic and Hypolipidemic Effects of Lycium barbarum Polysaccharide in Diabetic Rats[J].中草药(英文版),2015,7(4):310-315
[11]MAO F,XIAO B X,JIANG Z,et al.Anticancer effect of Lycium barbarum polysaccharides on colon cancer cells involves G0/G1 phase arrest[J].Medical Oncology,2011,28(1):121-126
[12]汪建红,陈晓琴,原惠.黑果枸杞果实多糖水浸提条件优化研究[J].新疆师范大学学报(自然科学版),2009,28(3):78-83
[13]张崇坚.宁夏枸杞的综合开发利用研究[D].武汉:湖北工业大学,2015
[14]谢晓霞,沈广军.新疆枸杞多糖的提取及其单糖组成分析[J].价值工程,2015(28):194-196
[15]陈亮,张炜,陈元涛,等.响应曲面法优化黑果枸杞多糖的超声提取工艺[J].食品科技,2015(1):220-227
[16]张自萍,黄文波.枸杞总黄酮和多糖的超声提取及含量测定[J].农业科学研究,2006,27(1):2 2-24
[17]倪慧,卿德刚,刘姿荣,等.新疆枸杞多糖粗粉提取工艺的研究[J].新疆中医药,2005,23(4):7-8
[18]师婷婷.生物酶与超滤纯化在枸杞多糖和总黄酮提取中的应用研究[D].兰州:甘肃中医药大学,2016
[19]白红进,汪河滨,褚志强,等.不同方法提取黑果枸杞多糖的研究[J].食品工业科技,2007,28(3):145-146
[20]邱志敏.富集多糖枸杞固体饮料关键技术研究[D].广州:华南理工大学,2012
[21]黄文书,冯作山,李瑾瑜.枸杞多糖提取条件的研究[J].食品与机械,2007,23(3):87-88
[22]许程剑,郭文波,赵玉华,等.新疆枸杞多糖提取与脱色工艺的研究[J].安徽农业科学,2012(5):2887-2889
[23]金迪,梁英,孙工兵,等.植物多糖提取技术的研究进展[J].黑龙江八一农垦大学学报,2011,23(5):76-79
[24]李丹丹.枸杞多糖的提取及其水解物的研究[D].济南:齐鲁工业大学,2014
[25]CHEN R Z,LI Y,DONG H,et al.Optimization of ultrasonic extraction process of polysaccharides from Ornithogalum Caudatum Ait and evaluation of its biological activities[J].Ultrasonics Sonochemistry,2012,19(6):1160-1168
[26]LI X,WANG Z,WANG L,et al.Ultrasonic-assisted extraction of polysaccharides from Hohenbuehelia serotina,by response surface methodology[J].International Journal of Biological Macromolecules,2012,51(4):523-530
[27]XIAOA J,SUN J,YAO L Y,et al.Physicochemical characteristics of ultrasonic extracted polysaccharides from cordyceps cephalosporium mycelia[J].International Journal of Biological Macromolecules,2012,51(1/2):64-69
[28]王航宇,刘金荣,但建明,等.新疆枸杞多糖的超声提取及含量测定[J].中药材,2002,25(1):42-43
[29]陈栋,周永传.酶法在中药提取中的应用和进展[J].中国中药杂志,2007,32(2):99
[30]申利红,王建森,李雅,等.植物多糖的研究及应用进展[J].中国农学通报,2011,27(2):349-352
[31]陈晓琴.黑果枸杞果实多糖的制备与抗疲劳、降血糖生物功效的研究[D].乌鲁木齐:新疆师范大学,2007
[32]鲁小静,冯艳波,陈晓瑞,等.响应面法优化黑果枸杞多糖的提取工艺研究[J].中国酿造,2013,32(6):79-83
[33]娄涛涛,金玲,陀扬凌,等.黑果枸杞多糖提取工艺及其含量测定中显色条件优化研究[J].亚太传统医药,2017,13(5):16-20
[34]刘树兴,杜丁,花俊丽.黑果枸杞多糖的分离纯化及抗氧化活性研究[J].食品工业,2016(12):134-137
[35]彭强,吕晓鹏,黄琳娟,等.黑果枸杞多糖的纯化工艺研究[J].西北农业学报,2012,21(2):121-126
[36]缪文玉,吴彩娥.微波萃取技术在食品中的研究进展[J].中国酿造,2008,27(2):1-3
[37]胡冠九,徐明华.微波萃取在环境有机样品分析中的应用[J].环境科技,1997(1):31-33
[38]孙平,刘可志,赵丰.枸杞多糖的提取及其残渣处理的研究[J].食品工业,2013(1):48-50
[39]韩红.枸杞多糖GPC检测、提取工艺优化及其稳定性研究[D].广州:华南农业大学,2016
[40]王月囡,辛广,翁霞.两种不同方法提取枸杞多糖的比较研究[J]鞍山师范学院学报,2010,12(4):39-43
[41]高春燕.枸杞多糖的提取分离技术及其特性研究[D].西安:陕西师范大学,2006
[42]蔡光华,王晓玲.高压脉冲电场提取枸杞多糖工艺[J].食品科学2012,33(8):43-48
[43]杜津昊,刘建飞,刘晓风,等.高速剪切技术辅助提取枸杞多糖工艺[J].食品工业,2017(3):128-132
[44]ZHAO C,YANG R F,QIU T Q.Ultrasound-enhanced subcritical water extraction of polysaccharides from Lycium barbarum L.[J].Separation&Purification Technology,2013,120:141-147
[45]魏殿文,庄泽帅,徐海军.高压脉冲电场法提取天然产物的研究进展[J].国土与自然资源研究,2009(3):88-89
[46]张平亮.新型食品高剪切分散乳化机的结构原理及其应用[J].食品工业,2010(6):81-83
[47]张文明,杨诗斌,宋明淦,等.剪切式均质机的结构与理论研究[J].食品与机械,2001(3):8-10
[48]YE G,WANG S,HENG P W,et al.Development and optimization of solid dispersion containing pellets of itraconazole prepared by high shear pelletization[J].International Journal of Pharmaceutics,2007,337(1):80-87
[49]魏鉴腾,裴栋,刘永峰,等.高速剪切辅助提取浒苔多糖的工艺研究[J].食品工业科技,2014,35(19):267-269
[50]TANAKA M,TAKAMIZU A,HOSHINO M,et al.Extraction of dietary fiber from Citrus junos peel with subcritical water[J].Food&Bioproducts Processing,2012,90(2):180-186
[51]LUO X,CUI J,ZHANG H,et al.Ultrasound assisted extraction of polyphenolic compounds from red sorghum(Sorghum bicolor L.)bran and their biological activities and polyphenolic compositions[J].Industrial Crops&Products,2018,112:296-304
[52]SMITH R M.Extractions with superheated water[J].Journal of Chromatography A,2002,975(1):31-46
[2]AMAGASE H,FARNSWORTH N R.A review of botanical characteristics,phytochemistry,clinical relevance in efficacy and safety of Lycium barbarum fruit(Goji)[J].Food Research International,2011,44(7):1702-1717
[3]JIN M L,HUANG Q S,ZHAO K,et al.Biological activities and potential health benefit effects of polysaccharides isolated from Lycium barbarum L.[J].International Journal of Biological Macromolecules,2013,54(1):16-23
[4]XIA G Q,XIN N,LIU W,et al.Inhibitory effect of Lycium barbarum polysaccharides on cell apoptosis and senescence is potentially mediated by the p53 signaling pathway[J].Molecular Medicine Reports,2014,9(4):1237-1241
[5]LI J,WANG L,LI J,et al.Lycium barbarum polysaccharide attenuates high-fat diet-induced hepatic steatosis by up-regulating SIRT1expression and deacetylase activity[J].Scientific Reports,2016,6:36209
[6]LIU S Y,CHEN L,LI X C,et al.Lycium barbarum polysaccharide protects diabetic peripheral neuropathy by enhancing autophagy via mTOR/p70S6K inhibition in Streptozotocin-induced diabetic rats[J].Journal of Chemical Neuroanatomy,2017,89:37-42
[7]Wu H T,He X J,Hong Y K,et al.Chemical characterization of Lycium barbarum polysaccharides and its inhibition against liver oxidative injury of high-fat mice[J].International Journal of Biological Macromolecules,2010,46(5):540-543
[8]XIAO J,LIONG E C,CHING Y P,et al.Lycium barbarum polysaccharides protect mice liver from carbon tetrachloride-inducedoxidative stress andnecroinflammation[J].Journal of Ethnopharmacology,2012,139(2):462-470
[9]KE M,ZHANG X J,HAN Z H,et al.Extraction,purification of Lycium barbarum polysaccharides and bioactivity of purified fraction[J].Carbohydrate Polymers,2011,86(1):136-141
[10]ZHAO R,JIN R,CHEN Y,et al.Hypoglycemic and Hypolipidemic Effects of Lycium barbarum Polysaccharide in Diabetic Rats[J].中草药(英文版),2015,7(4):310-315
[11]MAO F,XIAO B X,JIANG Z,et al.Anticancer effect of Lycium barbarum polysaccharides on colon cancer cells involves G0/G1 phase arrest[J].Medical Oncology,2011,28(1):121-126
[12]汪建红,陈晓琴,原惠.黑果枸杞果实多糖水浸提条件优化研究[J].新疆师范大学学报(自然科学版),2009,28(3):78-83
[13]张崇坚.宁夏枸杞的综合开发利用研究[D].武汉:湖北工业大学,2015
[14]谢晓霞,沈广军.新疆枸杞多糖的提取及其单糖组成分析[J].价值工程,2015(28):194-196
[15]陈亮,张炜,陈元涛,等.响应曲面法优化黑果枸杞多糖的超声提取工艺[J].食品科技,2015(1):220-227
[16]张自萍,黄文波.枸杞总黄酮和多糖的超声提取及含量测定[J].农业科学研究,2006,27(1):2 2-24
[17]倪慧,卿德刚,刘姿荣,等.新疆枸杞多糖粗粉提取工艺的研究[J].新疆中医药,2005,23(4):7-8
[18]师婷婷.生物酶与超滤纯化在枸杞多糖和总黄酮提取中的应用研究[D].兰州:甘肃中医药大学,2016
[19]白红进,汪河滨,褚志强,等.不同方法提取黑果枸杞多糖的研究[J].食品工业科技,2007,28(3):145-146
[20]邱志敏.富集多糖枸杞固体饮料关键技术研究[D].广州:华南理工大学,2012
[21]黄文书,冯作山,李瑾瑜.枸杞多糖提取条件的研究[J].食品与机械,2007,23(3):87-88
[22]许程剑,郭文波,赵玉华,等.新疆枸杞多糖提取与脱色工艺的研究[J].安徽农业科学,2012(5):2887-2889
[23]金迪,梁英,孙工兵,等.植物多糖提取技术的研究进展[J].黑龙江八一农垦大学学报,2011,23(5):76-79
[24]李丹丹.枸杞多糖的提取及其水解物的研究[D].济南:齐鲁工业大学,2014
[25]CHEN R Z,LI Y,DONG H,et al.Optimization of ultrasonic extraction process of polysaccharides from Ornithogalum Caudatum Ait and evaluation of its biological activities[J].Ultrasonics Sonochemistry,2012,19(6):1160-1168
[26]LI X,WANG Z,WANG L,et al.Ultrasonic-assisted extraction of polysaccharides from Hohenbuehelia serotina,by response surface methodology[J].International Journal of Biological Macromolecules,2012,51(4):523-530
[27]XIAOA J,SUN J,YAO L Y,et al.Physicochemical characteristics of ultrasonic extracted polysaccharides from cordyceps cephalosporium mycelia[J].International Journal of Biological Macromolecules,2012,51(1/2):64-69
[28]王航宇,刘金荣,但建明,等.新疆枸杞多糖的超声提取及含量测定[J].中药材,2002,25(1):42-43
[29]陈栋,周永传.酶法在中药提取中的应用和进展[J].中国中药杂志,2007,32(2):99
[30]申利红,王建森,李雅,等.植物多糖的研究及应用进展[J].中国农学通报,2011,27(2):349-352
[31]陈晓琴.黑果枸杞果实多糖的制备与抗疲劳、降血糖生物功效的研究[D].乌鲁木齐:新疆师范大学,2007
[32]鲁小静,冯艳波,陈晓瑞,等.响应面法优化黑果枸杞多糖的提取工艺研究[J].中国酿造,2013,32(6):79-83
[33]娄涛涛,金玲,陀扬凌,等.黑果枸杞多糖提取工艺及其含量测定中显色条件优化研究[J].亚太传统医药,2017,13(5):16-20
[34]刘树兴,杜丁,花俊丽.黑果枸杞多糖的分离纯化及抗氧化活性研究[J].食品工业,2016(12):134-137
[35]彭强,吕晓鹏,黄琳娟,等.黑果枸杞多糖的纯化工艺研究[J].西北农业学报,2012,21(2):121-126
[36]缪文玉,吴彩娥.微波萃取技术在食品中的研究进展[J].中国酿造,2008,27(2):1-3
[37]胡冠九,徐明华.微波萃取在环境有机样品分析中的应用[J].环境科技,1997(1):31-33
[38]孙平,刘可志,赵丰.枸杞多糖的提取及其残渣处理的研究[J].食品工业,2013(1):48-50
[39]韩红.枸杞多糖GPC检测、提取工艺优化及其稳定性研究[D].广州:华南农业大学,2016
[40]王月囡,辛广,翁霞.两种不同方法提取枸杞多糖的比较研究[J]鞍山师范学院学报,2010,12(4):39-43
[41]高春燕.枸杞多糖的提取分离技术及其特性研究[D].西安:陕西师范大学,2006
[42]蔡光华,王晓玲.高压脉冲电场提取枸杞多糖工艺[J].食品科学2012,33(8):43-48
[43]杜津昊,刘建飞,刘晓风,等.高速剪切技术辅助提取枸杞多糖工艺[J].食品工业,2017(3):128-132
[44]ZHAO C,YANG R F,QIU T Q.Ultrasound-enhanced subcritical water extraction of polysaccharides from Lycium barbarum L.[J].Separation&Purification Technology,2013,120:141-147
[45]魏殿文,庄泽帅,徐海军.高压脉冲电场法提取天然产物的研究进展[J].国土与自然资源研究,2009(3):88-89
[46]张平亮.新型食品高剪切分散乳化机的结构原理及其应用[J].食品工业,2010(6):81-83
[47]张文明,杨诗斌,宋明淦,等.剪切式均质机的结构与理论研究[J].食品与机械,2001(3):8-10
[48]YE G,WANG S,HENG P W,et al.Development and optimization of solid dispersion containing pellets of itraconazole prepared by high shear pelletization[J].International Journal of Pharmaceutics,2007,337(1):80-87
[49]魏鉴腾,裴栋,刘永峰,等.高速剪切辅助提取浒苔多糖的工艺研究[J].食品工业科技,2014,35(19):267-269
[50]TANAKA M,TAKAMIZU A,HOSHINO M,et al.Extraction of dietary fiber from Citrus junos peel with subcritical water[J].Food&Bioproducts Processing,2012,90(2):180-186
[51]LUO X,CUI J,ZHANG H,et al.Ultrasound assisted extraction of polyphenolic compounds from red sorghum(Sorghum bicolor L.)bran and their biological activities and polyphenolic compositions[J].Industrial Crops&Products,2018,112:296-304
[52]SMITH R M.Extractions with superheated water[J].Journal of Chromatography A,2002,975(1):31-46