受损金黄色葡萄球菌对检测不确定度的影响
|
摘要
为探讨受损金黄色葡萄球菌对定量检验工作的影响,本文采用GB法、AOAC法分别对热损伤与正常金黄色葡萄球菌进行重复检测,采用A类不确定度评定方法计算其不确定度,进而评估受损菌对当前检测方法的影响。损伤菌和正常菌用GB法的扩展不确定度分别是0.052和0.032,AOAC法的扩展不确定度分别是0.047和0.014,两种方法受损菌的扩展不确定度值均大于正常菌的值,实验表明损伤状态下菌体细胞的存在会引起检测不确定度的增加,检测人员对此应引起重视。
This study discussed the influence of injured S. aureus to daily quantitative detection work, by comparing the evaluation uncertainty result between normal and hot-injured S. aureus experiment. According to the method of GB 4789.10 2010 and AOAC 2003.07, we detected the normal and hot-injured S. aureus enumeration 15 times respectively, and calculated the uncertainty value with type A evaluation uncertainty method, then assessed the injured microorganisms impaction on current detection methods. The expanded uncertainty result of injured and normal S. aureus enumeration detected with GB method was 0.052 and 0.032, the other method values was 0.047 and 0.014, respectively. Both injured microorganisms expanded uncertainty value were lager than the normal ones. This assay presented injured microorganisms would increase the uncertainty in our work, and inspector need to pay seriously attention on it.
This study discussed the influence of injured S. aureus to daily quantitative detection work, by comparing the evaluation uncertainty result between normal and hot-injured S. aureus experiment. According to the method of GB 4789.10 2010 and AOAC 2003.07, we detected the normal and hot-injured S. aureus enumeration 15 times respectively, and calculated the uncertainty value with type A evaluation uncertainty method, then assessed the injured microorganisms impaction on current detection methods. The expanded uncertainty result of injured and normal S. aureus enumeration detected with GB method was 0.052 and 0.032, the other method values was 0.047 and 0.014, respectively. Both injured microorganisms expanded uncertainty value were lager than the normal ones. This assay presented injured microorganisms would increase the uncertainty in our work, and inspector need to pay seriously attention on it.
引文
[1]中国合格评定国家认可委员会.CNAS-CL07:2011测量不确定度评估和报告通用要求,2011.
[2]中华人民共和国卫生部.GB 29921-2013食品安全国家标准食品中致病菌限量[S].2013.
[3]曹小红,杨政,鲁梅芳,等.两种卫生指标菌在瓶装水中“活的非可培养状态”的研究[J].食品科学,2009,30(11):197-201.
[4]中国合格评定国家认可委员会.CNAS-GL05:2011测量不确定度要求的实施指南,2011.
[5]中华人民共和国卫生部.GB 4789.10-2010食品安全国家标准食品微生物学检验金黄色葡萄球菌检验[S].2010.
[6]AOAC.The official methods of analysis of AOAC international.2003,07.
[7]汤水平,王力清,李静芳,等.婴幼儿配方奶粉菌落总数检验中不确定度的评定[J].微生物学杂志,2009,29(5):100-103.
[8]林雅慧,陈海宁,陈红香,等.损伤菌对大肠菌群检测不确定度评价的影响[J].广东化工,2016,43(20):95-97.
[9]黄忠民,吕海鹏,艾志录,等.冷冻致亚致死损伤的金黄色葡萄球菌修复机制[J].微生物学报,2015,55(11):1409-1417.
[2]中华人民共和国卫生部.GB 29921-2013食品安全国家标准食品中致病菌限量[S].2013.
[3]曹小红,杨政,鲁梅芳,等.两种卫生指标菌在瓶装水中“活的非可培养状态”的研究[J].食品科学,2009,30(11):197-201.
[4]中国合格评定国家认可委员会.CNAS-GL05:2011测量不确定度要求的实施指南,2011.
[5]中华人民共和国卫生部.GB 4789.10-2010食品安全国家标准食品微生物学检验金黄色葡萄球菌检验[S].2010.
[6]AOAC.The official methods of analysis of AOAC international.2003,07.
[7]汤水平,王力清,李静芳,等.婴幼儿配方奶粉菌落总数检验中不确定度的评定[J].微生物学杂志,2009,29(5):100-103.
[8]林雅慧,陈海宁,陈红香,等.损伤菌对大肠菌群检测不确定度评价的影响[J].广东化工,2016,43(20):95-97.
[9]黄忠民,吕海鹏,艾志录,等.冷冻致亚致死损伤的金黄色葡萄球菌修复机制[J].微生物学报,2015,55(11):1409-1417.