毒死蜱对褐飞虱的亚致死效应研究
|
摘要
为探讨毒死蜱(Chlorpyrifos)对褐飞虱Nilaparvata lugens(Stl)的亚致死效应,本文采用稻茎浸渍法,分别以LC10和LC25浓度的毒死蜱胁迫褐飞虱3龄若虫,观察受胁迫后试虫发育历期、存活数、产卵量和雌虫寿命的变化情况,并组建经LC10、LC25浓度处理后F1代种群生命表,为褐飞虱的综合防治提供数据参考。结果表明:以毒死蜱LC10和LC25浓度分别处理褐飞虱3龄若虫,其F0代的孵化率与对照相比明显下降;处理组褐飞虱的种群相对适合度分别仅为0. 86和0. 74,表明亚致死浓度毒死蜱可有效抑制褐飞虱种群的增长;通过组建F1代生命表发现,亚致死浓度处理褐飞虱后,能够显著抑制孵化率。另外,处理后褐飞虱的存活率、羽化率、孵化率与对照组相比均有所降低,对褐飞虱的发育历期有轻度延长。根据研究结果推测,将毒死蜱用于防治水稻害虫时,毒死蜱不但对褐飞虱有致死作用,其亚致死浓度胁迫能进一步抑制下一代褐飞虱的种群增长。
Rice stem dipping method was used to investigate the sublethal effect of chlorpyrifos on Nilaparvata lugensus( Stl).Third instar nymphs of N. lugensus were treated with the sublethal chlorpyrifos concentrations.The change in developmental duration,fecundity and female longevity was recorded and the time specific life table of F1 generation population was established. The results showed that the hatching rate of the F0 generation was significantly lower than that in control group. The relative fitness of N. lugens in the treatment group was only 0. 86 and 0. 74,indicating that the sublethal concentration of chlorpyrifos could effectively inhibit the growth of the population of N. lugens. The F1 generation life table indicated that exposure to LC10 and LC25 could significantly inhibit the hatching rate of N. lugens. In addition,the survival rate,emergence rate and hatchability of N. lugens after treatments were lower than those of control group,and the developmental period of N. lugens was slightly prolonged. The results suggested that chlorpyrifos could not only kill N. lugens but also further inhibit the population growth of the next generations when using it to control rice pests.
Rice stem dipping method was used to investigate the sublethal effect of chlorpyrifos on Nilaparvata lugensus( Stl).Third instar nymphs of N. lugensus were treated with the sublethal chlorpyrifos concentrations.The change in developmental duration,fecundity and female longevity was recorded and the time specific life table of F1 generation population was established. The results showed that the hatching rate of the F0 generation was significantly lower than that in control group. The relative fitness of N. lugens in the treatment group was only 0. 86 and 0. 74,indicating that the sublethal concentration of chlorpyrifos could effectively inhibit the growth of the population of N. lugens. The F1 generation life table indicated that exposure to LC10 and LC25 could significantly inhibit the hatching rate of N. lugens. In addition,the survival rate,emergence rate and hatchability of N. lugens after treatments were lower than those of control group,and the developmental period of N. lugens was slightly prolonged. The results suggested that chlorpyrifos could not only kill N. lugens but also further inhibit the population growth of the next generations when using it to control rice pests.
引文
[1]韩丽敏.当前稻飞虱的防治误区与对策[J].现代农村科技,2017(12):26.
[2]王召,杨洪,金道超,等.贵州道真县褐飞虱发生规律[J].植物保护,2014,40(4):135-139.
[3]孙梦秋.湖南省褐飞虱迁飞轨迹及其高度变化成因[D].南京:南京信息工程大学,2015. 66-74
[4]王彦华,王鸣华.近年来我国水稻褐飞虱暴发原因及治理对策[J].农药科学与管理,2007,28(2):49-54.
[5]程家安,朱金良,祝增荣,等.稻田飞虱灾变与环境调控[J].环境昆虫学报,2008,30(2):176-182.
[6]王彦华,陈进,沈晋良,等.防治褐飞虱的高毒农药替代药剂的室内筛选及交互抗性研究[J].中国水稻科学,2008,22(5):519-526.
[7]余华洋.褐飞虱的抗药性监测及对氟啶虫胺腈的抗性风险评估[D].南京:南京农业大学,2015:1-2.
[8] Perveen F. Sublethal effects of chlorfluazuron on reproductivity and viability of Spodoptera litura(F.)(Lep. Noctuidae)[J].Journal of Applied Entomology,2010,124(5-6):223-231.
[9] James D G,Price T S. Fecundity in Twospotted Spider Mite(Acari:Tetranychidae)is Increased by Direct and Systemic Exposure to Imidacloprid[J]. Journal of Economic Entomology,2002,95(4):729-732.
[10]谢桂英,孙淑君,赵艳芹,等.亚致死浓度毒死蜱对小麦禾谷缢管蚜生长和繁殖的影响[J].农药学学报,2014,16(6):681-686.
[11]段辛乐,乔宪凤,陈茂华.亚致死浓度毒死蜱和异丙威对禾谷缢管蚜试验种群的影响[J].中国生态农业学报,2015,23(03):329-336.
[12]高君晓,梁沛,宋敦伦,等.高效氯氰菊酯亚致死剂量对大豆蚜实验种群的影响[J].植物保护学报,2008,35(4):379-380.
[13]段辛乐.二斑叶螨对甲氰菊酯和螺螨酯的抗性机理及两种药剂的亚致死效应研究[D].兰州:甘肃农业大学,2011:29-38.
[14]何恒果.桔全爪螨对甲氰菊酯和阿维菌素的抗性及其酯酶基因的克隆与表达研究[D].重庆:西南大学,2010:22-26.
[15]李锦钰,肖达,曲焱焱,等.马拉硫磷亚致死浓度对大豆蚜虫实验种群生理特征和生命表参数的影响[J].农药学学报,2014,16(2):119-124.
[16]杨保军,江云珠,刘淑华,等.几种杀虫剂亚致死剂量处理对褐飞虱繁殖力影响[C].粮食安全与植保科技创新,2009:1.
[17]蔡美艳,冯永斌,陈海波,等. 5%毒死蜱颗粒剂防治水稻不同生态位稻飞虱药效试验[J].湖北植保,2010(2):48-49.
[18]赵兴华.褐飞虱对苯基吡唑类杀虫剂抗性风险评估及交互抗性研究[D].南京:南京农业大学,2010:1-3.
[19]万秀秀.粘质沙雷氏菌对褐飞虱的亚致死效应[D].南京:南京农业大学,2015:1-5.
[20]庄永林,沈晋良.稻褐飞虱对噻嗪酮抗性的检测技术[J].南京农业大学学报,2000(3):114-117.
[21]王召,杨洪,金道超.两种方法测定氯虫苯甲酰胺对黑肩绿盲蝽的毒性[J].山地农业生物学报,2015,34(5):86-88.
[22]杨航,杨洪,金道超,等.噻虫嗪对白背飞虱试验种群繁殖力的亚致死效应[J].植物保护学报,2017,44(3):509-514.
[23]顾中言,徐德进,徐广春,等.稻田用杀虫剂对灰飞虱种群数量的影响[J].江苏农业学报,2014,30(6):1328-1335.
[24]汤爱兵.溴氰菊酯对稻褐飞虱繁殖力的影响[J].江西植保,2007,30(2):70-71.
[25]杨洪,王召,金道超.氯虫苯甲酰胺对白背飞虱实验种群的亚致死效应[J].昆虫学报,2012,55(10):1161-1167.
[26]杨洪,王召,金道超.氯虫苯甲酰胺对非靶标害虫褐飞虱实验种群的亚致死效应[J].应用生态学报,2013,24(2):549-555.
[27]杨燕涛,杨凌峰.毒死蜱、噻嗪酮对褐飞虱的防控效果分析[J].农药科学与管理,2007(8):24-27.
[2]王召,杨洪,金道超,等.贵州道真县褐飞虱发生规律[J].植物保护,2014,40(4):135-139.
[3]孙梦秋.湖南省褐飞虱迁飞轨迹及其高度变化成因[D].南京:南京信息工程大学,2015. 66-74
[4]王彦华,王鸣华.近年来我国水稻褐飞虱暴发原因及治理对策[J].农药科学与管理,2007,28(2):49-54.
[5]程家安,朱金良,祝增荣,等.稻田飞虱灾变与环境调控[J].环境昆虫学报,2008,30(2):176-182.
[6]王彦华,陈进,沈晋良,等.防治褐飞虱的高毒农药替代药剂的室内筛选及交互抗性研究[J].中国水稻科学,2008,22(5):519-526.
[7]余华洋.褐飞虱的抗药性监测及对氟啶虫胺腈的抗性风险评估[D].南京:南京农业大学,2015:1-2.
[8] Perveen F. Sublethal effects of chlorfluazuron on reproductivity and viability of Spodoptera litura(F.)(Lep. Noctuidae)[J].Journal of Applied Entomology,2010,124(5-6):223-231.
[9] James D G,Price T S. Fecundity in Twospotted Spider Mite(Acari:Tetranychidae)is Increased by Direct and Systemic Exposure to Imidacloprid[J]. Journal of Economic Entomology,2002,95(4):729-732.
[10]谢桂英,孙淑君,赵艳芹,等.亚致死浓度毒死蜱对小麦禾谷缢管蚜生长和繁殖的影响[J].农药学学报,2014,16(6):681-686.
[11]段辛乐,乔宪凤,陈茂华.亚致死浓度毒死蜱和异丙威对禾谷缢管蚜试验种群的影响[J].中国生态农业学报,2015,23(03):329-336.
[12]高君晓,梁沛,宋敦伦,等.高效氯氰菊酯亚致死剂量对大豆蚜实验种群的影响[J].植物保护学报,2008,35(4):379-380.
[13]段辛乐.二斑叶螨对甲氰菊酯和螺螨酯的抗性机理及两种药剂的亚致死效应研究[D].兰州:甘肃农业大学,2011:29-38.
[14]何恒果.桔全爪螨对甲氰菊酯和阿维菌素的抗性及其酯酶基因的克隆与表达研究[D].重庆:西南大学,2010:22-26.
[15]李锦钰,肖达,曲焱焱,等.马拉硫磷亚致死浓度对大豆蚜虫实验种群生理特征和生命表参数的影响[J].农药学学报,2014,16(2):119-124.
[16]杨保军,江云珠,刘淑华,等.几种杀虫剂亚致死剂量处理对褐飞虱繁殖力影响[C].粮食安全与植保科技创新,2009:1.
[17]蔡美艳,冯永斌,陈海波,等. 5%毒死蜱颗粒剂防治水稻不同生态位稻飞虱药效试验[J].湖北植保,2010(2):48-49.
[18]赵兴华.褐飞虱对苯基吡唑类杀虫剂抗性风险评估及交互抗性研究[D].南京:南京农业大学,2010:1-3.
[19]万秀秀.粘质沙雷氏菌对褐飞虱的亚致死效应[D].南京:南京农业大学,2015:1-5.
[20]庄永林,沈晋良.稻褐飞虱对噻嗪酮抗性的检测技术[J].南京农业大学学报,2000(3):114-117.
[21]王召,杨洪,金道超.两种方法测定氯虫苯甲酰胺对黑肩绿盲蝽的毒性[J].山地农业生物学报,2015,34(5):86-88.
[22]杨航,杨洪,金道超,等.噻虫嗪对白背飞虱试验种群繁殖力的亚致死效应[J].植物保护学报,2017,44(3):509-514.
[23]顾中言,徐德进,徐广春,等.稻田用杀虫剂对灰飞虱种群数量的影响[J].江苏农业学报,2014,30(6):1328-1335.
[24]汤爱兵.溴氰菊酯对稻褐飞虱繁殖力的影响[J].江西植保,2007,30(2):70-71.
[25]杨洪,王召,金道超.氯虫苯甲酰胺对白背飞虱实验种群的亚致死效应[J].昆虫学报,2012,55(10):1161-1167.
[26]杨洪,王召,金道超.氯虫苯甲酰胺对非靶标害虫褐飞虱实验种群的亚致死效应[J].应用生态学报,2013,24(2):549-555.
[27]杨燕涛,杨凌峰.毒死蜱、噻嗪酮对褐飞虱的防控效果分析[J].农药科学与管理,2007(8):24-27.