3种增效剂与3种烟碱类杀虫剂混配对棉蚜的增效作用
|
摘要
【目的】分析棉蚜对传统烟碱类杀虫剂吡虫啉、啶虫脒、噻虫嗪的敏感性,研究青皮桔柚、有机硅、激健3种增效剂对棉蚜的增效作用。【方法】采用浸叶法测,定3种增效剂与3种烟碱类杀虫剂混配后对棉蚜的生物活性及增效作用。【结果】吡虫啉、啶虫脒、噻虫嗪对棉蚜24 h LC_(50)分别为11.878、24.452、12.110μg/mL,吡虫啉和噻虫嗪相对毒力分别是啶虫脒的2.059和2.019倍。添加3种增效剂后,9种农药+增效剂组合对棉蚜的毒力均有显著提高,其中吡虫啉+青皮桔油、啶虫脒+有机硅、噻虫嗪+青皮桔油的组合增效作用最佳,对棉蚜24 h LC_(50)分别为0.328、1.987、0.704μg/mL,增效比分别为36.213、12.306、17.202。【结论】青皮桔柚、有机硅、激健3种增效剂在新疆棉区棉蚜绿色防控和农药减施增效技术可应用。
【Objective】 This study aims to study the sensitivity of Aphis gossypii to traditional nicotine insecticides imidacloprid, acetamiprid and thiothiazide and clarify and synergistic effects of the three synergists: green peel orange oil(GPOO), organosilicon(OSi) and Jijian adjuvant.(JJ, active ingredients: corn germ oil, rapeseed oil and soybean oil, etc.) mixed with imidacloprid, acetamiprid and thiamethoxam on Aphis gossypii.【Method】The bioactivity and synergistic effect of three synergists mixed with three nicotine insecticides on Aphis gossypii were determined by leaf soaking method. 【Result】After 24 hours of treatment, the LC_(50 )of imidacloprid, acetamiprid and thiamethoxam to Aphis gossypii were 11.878, 24.452 and 12.110 μg/mL, respectively. The relative toxicities of imidacloprid and thiamethoxam were 2.059 and 2.019 times higher than those of acetamiprid, respectively. After adding three synergists, the bioassay of nine combinations of pesticide+synergist to Aphis gossypii was significantly increased. Among them, imidacloprid+GPOO, acetamiprid+OSi, thiamethoxam+GPOO had the best synergistic effect. After 24 hours of treatment, the LC_(50 )were 0.328, 1.987, 0.704 μg/mL, and the synergistic ratio was 36.213, 12.306 and 17.202, respectively. 【Conclusion】Green peel orange oil, organosilicon and Jijian adjuvant have good potential in the application of green control and pesticide reduction and synergism technology for cotton Aphis gossypii in Xinjiang.
【Objective】 This study aims to study the sensitivity of Aphis gossypii to traditional nicotine insecticides imidacloprid, acetamiprid and thiothiazide and clarify and synergistic effects of the three synergists: green peel orange oil(GPOO), organosilicon(OSi) and Jijian adjuvant.(JJ, active ingredients: corn germ oil, rapeseed oil and soybean oil, etc.) mixed with imidacloprid, acetamiprid and thiamethoxam on Aphis gossypii.【Method】The bioactivity and synergistic effect of three synergists mixed with three nicotine insecticides on Aphis gossypii were determined by leaf soaking method. 【Result】After 24 hours of treatment, the LC_(50 )of imidacloprid, acetamiprid and thiamethoxam to Aphis gossypii were 11.878, 24.452 and 12.110 μg/mL, respectively. The relative toxicities of imidacloprid and thiamethoxam were 2.059 and 2.019 times higher than those of acetamiprid, respectively. After adding three synergists, the bioassay of nine combinations of pesticide+synergist to Aphis gossypii was significantly increased. Among them, imidacloprid+GPOO, acetamiprid+OSi, thiamethoxam+GPOO had the best synergistic effect. After 24 hours of treatment, the LC_(50 )were 0.328, 1.987, 0.704 μg/mL, and the synergistic ratio was 36.213, 12.306 and 17.202, respectively. 【Conclusion】Green peel orange oil, organosilicon and Jijian adjuvant have good potential in the application of green control and pesticide reduction and synergism technology for cotton Aphis gossypii in Xinjiang.
引文
[1] 郭天凤,马野萍,丁荣荣,等.新疆主要植棉区棉蚜对吡虫啉和啶虫脒的抗性评价[J].中国棉花,2012,39(12):4-6.GUO Tian-feng,MA Ye-ping,DING Rong-rong,et al.(2012).Resistance of the Aphis gossypii populations from main cotton area in Xinjiang to imidacloprid and acetamiprid [J].China Cotton,39(12):4-6.(in Chinese)
[2] 柳建伟,张东海,陈晋忠,等.棉蚜对12种杀虫剂的敏感性测定[J].农药,2012,51(8):613-615.LIU Jian-wei,ZHANG Dong-hai,CHEN Jin-zhong,et al.(2012).Determination of Sensitivity of Aphis gossypii to 12 Insecticides [J].Agrochemicals,51(8):613-615.(in Chinese)
[3] 郭天凤,周晶,叶玉霞,等.棉蚜啶虫脒不同品系解毒酶活性测定和增效剂作用的研究[J].新疆农业科学,2015,52(7):1 334-1 339.GUO Tian-feng,ZHOU Jing,YE Yu-xia,et al.(2015).Study on the activity of the detoxification enzymes and synergist synergism of cotton aphid (Aphis gossypii) on acetamiprid of different strains [J].Xinjiang Agricultural Sciences,52(7):1,334-1,339.(in Chinese)
[4] 崔丽,张靖,齐浩亮,等.我国棉花主产区棉蚜对吡虫啉的抗性监测及抗性机理[J].昆虫学报,2016,59(11):1 246-1 253.CUI Li,ZHANG Jing,QI Hao-liang,et al.(2016).Monitoring and mechanisms of imidacloprid resistance in Aphis gossypii (Hemiptera:Aphidiidae) in the main cotton production areas of China [J].Acta Entomologica Sinica,59(11):1,246-1,253.(in Chinese)
[5] 赵鹏程,李焱,闫文静,等.新疆棉蚜不同地理种群对杀虫剂的敏感性[J].石河子大学学报(自然科学版),2018,36(2):159-163.ZHAO Peng-cheng,LI Yan,YAN Wen-jing,et al.(2018).Sensitivity of different geographical populations of Aphis gossypii (Glover) in Xinjiang to different insecticides [J].Journal of Shihezi University (Natural Science Ed.),36(2):159-163.(in Chinese)
[6] 李进,张军高,王伟,等.不同增效剂对噻虫嗪防治棉蚜的减量增效作用[J].农药,2018,57(12):912-915.LI Jin,ZHANG Jun-gao,WANG Wei,et al.(2018).Decrement and synergistic effect of synergists in preventing cotton aphids with thiamethoxam [J].Agrochemicals,57(12):912-915.(in Chinese)
[7] Entwistle,G.,Rees,T.A.,& Rees,T.A.(1988).Enzymic capacities of amyloplasts from wheat (Triticum aestivum) endosperm.Biochemical Journal,255(2):391-396.
[8] 张学涛,柳建伟,李芬,等.北疆地区棉蚜对不同杀虫剂敏感度水平测定[J].植物保护,2012,38(2):163-166.ZHANG Xue-tao,LIU Jian-wei,LI Fen,et al.(2012).Susceptible level of Aphis gossypii to different insecticides in the north of Xinjiang [J].Plant Protection,38(2):163-166.(in Chinese)
[9] 郭天凤,史雪岩,高希武,等.棉蚜啶虫脒抗性种群交互抗性和增效剂增效作用的研究[J].应用昆虫学报,2014,51(3):819-826.GUO Tian-feng,SHI Xue-yan,GAO Xi-wu,et al.(2014).Cross-resistance to,and synergists of,acetamiprid in Aphis gossypii [J].Chinese Journal of Applied Entomology,51(3):819-826.(in Chinese)
[10] 郭天凤.新疆棉区棉蚜对新烟碱类杀虫药剂抗性监测、风险评估及抗性机制的研究[D].北京:中国农业大学博士论文,2014.GUO Tian-feng.(2014).Monitoring,risk assessment and mechanisms of Aphis gossypii (Glover) resistance to neonicotinoid insecticides in Xinjiang [D].PhD Dissertation.China Agricultural University,Beijing.(in Chinese)
[11] 张国福.棉蚜对吡虫啉的代谢抗性分子机制研究[D].泰安:山东农业大学硕士论文,2015.ZHANG Guo-fu.(2015).Molecular mechanisms of metabolism for imidacloprid resistance in Aphis gossypii[D].Master Dissertation.Shandong Agricultural University,Taian.(in Chinese)
[2] 柳建伟,张东海,陈晋忠,等.棉蚜对12种杀虫剂的敏感性测定[J].农药,2012,51(8):613-615.LIU Jian-wei,ZHANG Dong-hai,CHEN Jin-zhong,et al.(2012).Determination of Sensitivity of Aphis gossypii to 12 Insecticides [J].Agrochemicals,51(8):613-615.(in Chinese)
[3] 郭天凤,周晶,叶玉霞,等.棉蚜啶虫脒不同品系解毒酶活性测定和增效剂作用的研究[J].新疆农业科学,2015,52(7):1 334-1 339.GUO Tian-feng,ZHOU Jing,YE Yu-xia,et al.(2015).Study on the activity of the detoxification enzymes and synergist synergism of cotton aphid (Aphis gossypii) on acetamiprid of different strains [J].Xinjiang Agricultural Sciences,52(7):1,334-1,339.(in Chinese)
[4] 崔丽,张靖,齐浩亮,等.我国棉花主产区棉蚜对吡虫啉的抗性监测及抗性机理[J].昆虫学报,2016,59(11):1 246-1 253.CUI Li,ZHANG Jing,QI Hao-liang,et al.(2016).Monitoring and mechanisms of imidacloprid resistance in Aphis gossypii (Hemiptera:Aphidiidae) in the main cotton production areas of China [J].Acta Entomologica Sinica,59(11):1,246-1,253.(in Chinese)
[5] 赵鹏程,李焱,闫文静,等.新疆棉蚜不同地理种群对杀虫剂的敏感性[J].石河子大学学报(自然科学版),2018,36(2):159-163.ZHAO Peng-cheng,LI Yan,YAN Wen-jing,et al.(2018).Sensitivity of different geographical populations of Aphis gossypii (Glover) in Xinjiang to different insecticides [J].Journal of Shihezi University (Natural Science Ed.),36(2):159-163.(in Chinese)
[6] 李进,张军高,王伟,等.不同增效剂对噻虫嗪防治棉蚜的减量增效作用[J].农药,2018,57(12):912-915.LI Jin,ZHANG Jun-gao,WANG Wei,et al.(2018).Decrement and synergistic effect of synergists in preventing cotton aphids with thiamethoxam [J].Agrochemicals,57(12):912-915.(in Chinese)
[7] Entwistle,G.,Rees,T.A.,& Rees,T.A.(1988).Enzymic capacities of amyloplasts from wheat (Triticum aestivum) endosperm.Biochemical Journal,255(2):391-396.
[8] 张学涛,柳建伟,李芬,等.北疆地区棉蚜对不同杀虫剂敏感度水平测定[J].植物保护,2012,38(2):163-166.ZHANG Xue-tao,LIU Jian-wei,LI Fen,et al.(2012).Susceptible level of Aphis gossypii to different insecticides in the north of Xinjiang [J].Plant Protection,38(2):163-166.(in Chinese)
[9] 郭天凤,史雪岩,高希武,等.棉蚜啶虫脒抗性种群交互抗性和增效剂增效作用的研究[J].应用昆虫学报,2014,51(3):819-826.GUO Tian-feng,SHI Xue-yan,GAO Xi-wu,et al.(2014).Cross-resistance to,and synergists of,acetamiprid in Aphis gossypii [J].Chinese Journal of Applied Entomology,51(3):819-826.(in Chinese)
[10] 郭天凤.新疆棉区棉蚜对新烟碱类杀虫药剂抗性监测、风险评估及抗性机制的研究[D].北京:中国农业大学博士论文,2014.GUO Tian-feng.(2014).Monitoring,risk assessment and mechanisms of Aphis gossypii (Glover) resistance to neonicotinoid insecticides in Xinjiang [D].PhD Dissertation.China Agricultural University,Beijing.(in Chinese)
[11] 张国福.棉蚜对吡虫啉的代谢抗性分子机制研究[D].泰安:山东农业大学硕士论文,2015.ZHANG Guo-fu.(2015).Molecular mechanisms of metabolism for imidacloprid resistance in Aphis gossypii[D].Master Dissertation.Shandong Agricultural University,Taian.(in Chinese)