28个农药产品推荐剂量对西瓜噬酸菌的抑制活性
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摘要
西瓜噬酸菌(Acidovorax citrulli,简称Ac)引起的西瓜细菌性果斑病(Bacterial Fruit Blotch,简称BFB)是西瓜和甜瓜生产上重要的毁灭性种传细菌病害。一旦发病,用于该病害防治的有效药剂的选择就极为重要。为了筛选出高防效药剂,笔者采用抑菌圈法测定了乙酸素等28个农药产品的推荐剂量对2株西瓜噬酸菌的抑制活性。试验结果表明,供试药剂0.3%四霉素AS稀释30倍液和50倍液、30%乙蒜素EC 500倍液和700倍液、80%乙蒜素EC 1 500倍液和1 800倍液对供试菌株AacJ-N和Aac7500的抑菌活性较好,抑菌圈直径均在15 mm以上;46%氢氧化铜WG 700倍液和1 000倍液、53.8%氢氧化铜WG 500倍液和600倍液、20%溴硝醇WP 1 000倍液和1 500倍液、2%春雷·45%王铜WP(江门植保)470倍液和750倍液、3%噻霉酮ME 360倍液对供试菌株AacJ-N和Aac7500的抑菌圈直径在10～15 mm;其余供试药剂在试验剂量下均无抑菌作用。不同菌株对相同药剂同一浓度的敏感性存在差异。
Bacterial fruit blotch caused by Acidovorax citrulli is a destructive disease of watermelon and melon. The selection of the control agents is very important when once it occurred. In order to screen out high efficiency control agent, the inhibitory effect of 28 reagents against two A. citrulli strains was determined by using inhibitory halo method. The inhibitory haloes of 30-and 50-fold dilution of 0.3% Tetramycin AS,500-and 700-fold dilution of 30% Ethylicin AS, 1 500 and1 800-fold dilution of 80% Ethylicin were above 15 mm against strains AacJ-N and Aac7500. The inhibitory haloes of700-and 1 000-fold dilution of 46% Sodium hydroxide WG, 500-and 600-fold dilution of 53.8% Sodium hydroxide WG,1 000-and 1 500-fold dilutions of 20% Bronopo WPl, 470-and 750-fold dilutions of 2% kasugamycin · 45% Copper(Ⅱ)busic chloride WP(Jiangmen plant protection Co., Ltd.), 360-fold dilution of 3% benziothiazolinone ME were ranged from 10-15 mm against strains AacJ-N and Aac7500, while rest of the tested reagents did not show any inhibitory effects.Additionally, the two strains showed different resistance to the same reagent at same concentration.
Bacterial fruit blotch caused by Acidovorax citrulli is a destructive disease of watermelon and melon. The selection of the control agents is very important when once it occurred. In order to screen out high efficiency control agent, the inhibitory effect of 28 reagents against two A. citrulli strains was determined by using inhibitory halo method. The inhibitory haloes of 30-and 50-fold dilution of 0.3% Tetramycin AS,500-and 700-fold dilution of 30% Ethylicin AS, 1 500 and1 800-fold dilution of 80% Ethylicin were above 15 mm against strains AacJ-N and Aac7500. The inhibitory haloes of700-and 1 000-fold dilution of 46% Sodium hydroxide WG, 500-and 600-fold dilution of 53.8% Sodium hydroxide WG,1 000-and 1 500-fold dilutions of 20% Bronopo WPl, 470-and 750-fold dilutions of 2% kasugamycin · 45% Copper(Ⅱ)busic chloride WP(Jiangmen plant protection Co., Ltd.), 360-fold dilution of 3% benziothiazolinone ME were ranged from 10-15 mm against strains AacJ-N and Aac7500, while rest of the tested reagents did not show any inhibitory effects.Additionally, the two strains showed different resistance to the same reagent at same concentration.
引文
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[6]蔡学清,黄月英,杨建珍,等.福建省西瓜细菌性果斑病的病原鉴定[J].福建农业大学学报,2005(4):434-437.
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[13]韩盛,杨渡,玉山江·麦麦提,等.新疆籽瓜细菌性果斑病的发生与分子鉴定[J].新疆农业科学,2015,52(9):1631-1639.
[14]赵廷昌,孙福在,王建荣,等.药剂处理种子防治哈密瓜细菌性果斑病[J].植物保护,2003,29(4):50-53.
[15]杜艳媚,王琳,李小妮,等.西瓜细菌性果斑病带菌部位检测及种子处理研究[J].广东农业科学,2011,38(19):121-123.
[16]何毅,韦志扬,李文信,等.药物浸种处理防治西瓜嫁接苗细菌性果斑病试验[J].南方农业学报,2011,42(5):500-504.
[17]贾丽慧,刘建华,周勇.新疆西瓜甜瓜杂交制种细菌性果斑病的发生与防治[J].中国瓜菜,2012,25(4):63-64.
[18]宋顺华,吴萍,孟淑春,等.种子处理对西瓜细菌性果斑病的防治效果[J].中国瓜菜,2013,26(3):5-9.
[19]贾云鹤,王喜庆,付永凯,等.黑龙江省西瓜细菌性果斑病防治药剂的筛选[J].中国瓜菜,2015,28(3):34-37.
[20]黄武仁,文衍堂.西瓜细菌性果斑病菌的室内药剂筛选试验[J].华南热带农业大学学报,2001(3):10-11.
[21]王雪,王秀华,刘晓畅,等.三十七种生物农药对西瓜细菌性果斑病菌的室内抑菌效果测定[J].北方园艺,2015(19):111-113.
[22]王雪,高洁,张静,等. 63种杀菌剂对西瓜、甜瓜细菌性果斑病菌的室内毒力测定[J].吉林农业大学学报,2012,34(6):612-617.
[23]王中武,吴广成.西瓜细菌性果斑病药剂防治试验[J].北方园艺,2009(1):109-110.
[24]金岩,张俊杰,高洁.西瓜细菌性果斑病化学药剂防治研究[J].北方园艺,2010(2):193-195.
[25]武芳,李可,王云鹏,等.生物农药“蔬得康”对西瓜细菌性果斑病的温室防效评估[J].中国生物防治学报,2014,30(2):266-270.
[26]万秀琴,王惠林.不同药剂对瓜类幼苗细菌性果斑病防治效果比较[J].北方园艺,2017(12):129-132.
[2] SOMODI G C,JONES J B,HOPKINS D L,et al. Occurrence of a bacterial watermelon fruit blotch in Florida[J]. Plant disease,1991,75(10):1053-1056.
[3] RANE K K,LATIN R X. Bacterial fruit blotch of watermelon:Association of the pathogen with seed[J]. Plant disease,1992,76(5):509-512.
[4]赵廷昌,孙福在,王兵万.西瓜细菌性果斑病研究进展[J].植保技术与推广,2001(3):37-38.
[5]金岩,张俊杰,吴燕华,等.西瓜细菌性果斑病的发生与病原菌鉴定[J].吉林农业大学学报,2004(3):263-266.
[6]蔡学清,黄月英,杨建珍,等.福建省西瓜细菌性果斑病的病原鉴定[J].福建农业大学学报,2005(4):434-437.
[7]赵廷昌,赵洪海,王怀松.山东省西瓜、甜瓜发生瓜类细菌性果斑病[J].植物保护,2009,35(5):170-171.
[8]高天一.湖北省西瓜细菌性果斑病病原鉴定[A].中国植物病理学会2011年学术年会论文集[C].中国植物病理学会,2011.
[9]安心,孙柏欣,刘志恒,等.辽宁省西瓜发生瓜类细菌性果斑病[J].中国蔬菜,2013(23):22-23.
[10]王文博,刘东,张艳菊.黑龙江省西瓜细菌性果斑病发生初报[J].中国蔬菜,2013(2):82-86.
[11]朱晓媛,王文成,徐烨,等.云南省瓜类细菌性果斑病的鉴定[J].云南农业大学学报(自然科学),2013,28(2):175-179.
[12]张悦丽,齐军山,张博,等.山东省西瓜细菌性果斑病的病原菌鉴定[J].山东农业科学,2014,46(3):83-85.
[13]韩盛,杨渡,玉山江·麦麦提,等.新疆籽瓜细菌性果斑病的发生与分子鉴定[J].新疆农业科学,2015,52(9):1631-1639.
[14]赵廷昌,孙福在,王建荣,等.药剂处理种子防治哈密瓜细菌性果斑病[J].植物保护,2003,29(4):50-53.
[15]杜艳媚,王琳,李小妮,等.西瓜细菌性果斑病带菌部位检测及种子处理研究[J].广东农业科学,2011,38(19):121-123.
[16]何毅,韦志扬,李文信,等.药物浸种处理防治西瓜嫁接苗细菌性果斑病试验[J].南方农业学报,2011,42(5):500-504.
[17]贾丽慧,刘建华,周勇.新疆西瓜甜瓜杂交制种细菌性果斑病的发生与防治[J].中国瓜菜,2012,25(4):63-64.
[18]宋顺华,吴萍,孟淑春,等.种子处理对西瓜细菌性果斑病的防治效果[J].中国瓜菜,2013,26(3):5-9.
[19]贾云鹤,王喜庆,付永凯,等.黑龙江省西瓜细菌性果斑病防治药剂的筛选[J].中国瓜菜,2015,28(3):34-37.
[20]黄武仁,文衍堂.西瓜细菌性果斑病菌的室内药剂筛选试验[J].华南热带农业大学学报,2001(3):10-11.
[21]王雪,王秀华,刘晓畅,等.三十七种生物农药对西瓜细菌性果斑病菌的室内抑菌效果测定[J].北方园艺,2015(19):111-113.
[22]王雪,高洁,张静,等. 63种杀菌剂对西瓜、甜瓜细菌性果斑病菌的室内毒力测定[J].吉林农业大学学报,2012,34(6):612-617.
[23]王中武,吴广成.西瓜细菌性果斑病药剂防治试验[J].北方园艺,2009(1):109-110.
[24]金岩,张俊杰,高洁.西瓜细菌性果斑病化学药剂防治研究[J].北方园艺,2010(2):193-195.
[25]武芳,李可,王云鹏,等.生物农药“蔬得康”对西瓜细菌性果斑病的温室防效评估[J].中国生物防治学报,2014,30(2):266-270.
[26]万秀琴,王惠林.不同药剂对瓜类幼苗细菌性果斑病防治效果比较[J].北方园艺,2017(12):129-132.