新型复合型生物种衣剂SN102田间防效研究
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摘要
为了安全有效地解决大豆苗期根部多种病害复合侵染的问题,利用微生物多样性及诱导抗病性理论,在辽宁省沈阳康平基地与黑龙江大庆基地,对新研发的一种兼抗大豆胞囊线虫与根腐病的新型复合型生物种衣剂SN102进行了为期2年的田间防治效果验证试验。生物种衣剂SN102是由本实验室自主研发,由巨大芽孢杆菌(Bacillus megaterium)Sneb482、简单芽孢杆菌(Bacillus simplex)Sneb545、费氏中华根瘤菌(Sinorhizobium fredii)Sneb183和产黄青霉菌(Penicillium chrysogenum)Snef805 4株生防菌株作为有效活性物质组合混配制成。结果表明:SN102对大豆幼苗生长有一定的促进作用,两年的平均胞囊抑制率分别为37.80%(康平)和28.95%(大庆),对大豆幼苗根腐病的平均防效分别为16.31%(康平)和29.57%(大庆),同时对大豆的平均增产率分别达到21.10%(康平)和12.36%(大庆)。SN102在不同地域的防病增产效果较稳定,具有很高的实际应用价值和开发潜力。
Soybean cyst nematode and root rot disease are two economically important soil-borne diseases in the northeast of China. In order to alleviate the incidence of these diseases during seedling stage,we designed a novel biological seed-coating agent SN102 by mixing the fermentation filtrate of Bacillus megaterium Sneb482,B. simplex Sneb545,Sinorhizobium fredii Sneb183 and Penicillium chrysogenum Snef805 with additives. Then we evaluated its effectiveness in the greenhouse and soybean fields of Kangping county in Liaoning province and Daqing city in Heilongjiang province,China in 2015 and in 2016.The result showed that SN102 was able to promote soybean growth during seedling stage. Application of SN102 inhibited the incidence of soybean cyst nematode by 37. 80% at Kangping and by 28. 95% at Daqing,and also of root rot disease by16. 31% at Kangping and by 29. 57% at Daqing over two years. As a result,the average yield of soybean was increased by21. 10% at Kangping and by 12. 36% at Daqing in two years. The encouraging effect of SN102 on biological control suggested that it had the potential to be applied widely in the soybean field in the future.
Soybean cyst nematode and root rot disease are two economically important soil-borne diseases in the northeast of China. In order to alleviate the incidence of these diseases during seedling stage,we designed a novel biological seed-coating agent SN102 by mixing the fermentation filtrate of Bacillus megaterium Sneb482,B. simplex Sneb545,Sinorhizobium fredii Sneb183 and Penicillium chrysogenum Snef805 with additives. Then we evaluated its effectiveness in the greenhouse and soybean fields of Kangping county in Liaoning province and Daqing city in Heilongjiang province,China in 2015 and in 2016.The result showed that SN102 was able to promote soybean growth during seedling stage. Application of SN102 inhibited the incidence of soybean cyst nematode by 37. 80% at Kangping and by 28. 95% at Daqing,and also of root rot disease by16. 31% at Kangping and by 29. 57% at Daqing over two years. As a result,the average yield of soybean was increased by21. 10% at Kangping and by 12. 36% at Daqing in two years. The encouraging effect of SN102 on biological control suggested that it had the potential to be applied widely in the soybean field in the future.
引文
[1]李沐慧,王媛媛,陈井生,等.2015年东北地区大豆田病害种类与危害程度调查研究[J].大豆科学,2016,35(4):643-648,671.(Li M H,Wang Y Y,Chen J S,et al.Incidence and disease index of soybean diseases in the northeast of China in 2015[J].Soybean Science,2016,35(4):643-648,671.)
[2]梁喜龙,郑殿峰,左豫虎.大豆根腐病的研究现状及展望[J].黑龙江八一农垦大学学报,2003,15(4):30-34.(Liang X L,Zheng D F,Zuo Y H.The status of the study and prospect on soybean root rot[J].Journal of Heilongjiang August First Land Reclamation University,2003,15(4):30-34.)
[3]段玉玺,陈立杰.大豆胞囊线虫病及其防治[M].北京:金盾出版社,2006:6-7.(Duan Y X,Chen L J.Soybean cyst nematode and its control[M].Beijing:Jindun Publishing House,2006:6-7.)
[4]武亚敬,张金香,高广瑞,等.我国种衣技术的研究进展[J].作物杂志,2007(4):62-66.(Wu J Y,Zhang J X,Gao G R,et al.Research progress on the technology of Chinese seed coating[J].Crops,2007(4):62-66.)
[5]Saikia S K,Tiwari S,Pandey R.Rhizospheric biological weapons for growth enhancement and Meloidogyne incognita,management in Withania somnifera cv.Poshita[J].Biological Control,2013,65(2):225-234.
[6]Akhtar A,Hisamuddin,Robab M I,et al.Plant growth promoting Rhizobacteria:An overview[J].Journal of Natural Product&Plant Resources,2012,2(1):19-31.
[7]郭宁,陈立杰,段玉玺,等.控制大豆胞囊线虫生物种衣剂的研制及沈阳田间小区防效的初步试验[J].植物保护,2009,35(2):158-162.(Guo N,Chen L J,Duan Y X,et al.Preparation and effects of biological seed coating on the soybean cyst nematode in soybean plots in Shenyang[J].Plant Protection,2009,35(2):158-162.)
[8]陈立杰,万传浩,朱晓峰,等.Snea253生物种衣剂防治大豆胞囊线虫的研究[J].大豆科学,2011,30(3):459-462.(Chen L J,Wan C H,Zhu X F,et al.Control effects of Snea253biological seed coating on soybean cyst nematode[J].SoybeanScience,2011,30(3):459-462.)
[9]周园园,王媛媛,朱晓峰,等.生物种衣剂SN101的研制及其对大豆胞囊线虫病的防效[J].中国油料作物学报,2014,36(4):513-518.(Zhou Y Y,Wang Y Y,Zhu X F,et al.Development of a biological seed-coating preparation and it’s efficiency in prevention of Heterodera glycines[J].Chinese Journal of Oil Crop Science,2014,36(4):513-518.)
[10]孙华,段玉玺,焦石,等.抗大豆胞囊线虫的根际促生菌的筛选及其鉴定[J].大豆科学,2009,28(3):507-510.(Sun H,Duan Y X,Jiao S,et al.Filtration and identification of plant growth promoting Rhizobacteria on resistance of soybean cyst nematode[J].Soybean Science,2009,28(3):507-510.)
[11]项鹏,陈立杰,朱晓峰,等.种子处理诱导大豆抗胞囊线虫病的生防细菌筛选与鉴定[J].中国生物防治学报,2013,29(4):661-666.(Xiang P,Chen L J,Zhu X F,et al.Screening and identification of bacterium to induce resistance of soybean against Heterodera glycines[J].Chinese Journal of Biological Control,2013,29(4):661-666.)
[12]田丰,陈立杰,王媛媛,等.根瘤菌Sneb183对大豆胞囊线虫二龄幼虫的作用方式研究[J].中国生物防治学报,2014,30(4):540-545.(Tian F,Chen L J,Wang Y Y,et al.Action mode of Sinorhizobium fredii Sneb183 on the activity of soybean cyst nematode[J],Chinese Journal of Biological Control,2014,30(4):540-545.)
[13]闫继辰,王媛媛,朱晓峰,等.不同真菌发酵液包衣种子对大豆生长和抗病性的影响[J].沈阳农业大学学报,2016,47(4):406-411.(Yan J C,Wang Y Y,Zhu X F,et al.Effect of seed coating with different fungus fermentation liquid on the growth and disease resistance of soybean[J].Journal of Shenyang Agricultural University,2016,47(4):406-411.)
[14]顾鑫,孙力,于铭,等.新型缓释甲霜灵颗粒剂对大豆根腐病主要致病菌的防治效果研究[J].农学学报,2016,30(3):12-15.(Gu X,Sun L,Yu M,et al.Control efficiency of a new type controlled-release metalaxyl granule on main pathogenic bacteria of root rot of soybean[J].Journal of Agriculture,2016,30(3):12-15.)
[15]闫继辰,王媛媛,朱晓峰,等.诱导大豆抗胞囊线虫病和根腐病的生防细菌研究[J].安徽农业科学,2016,44(9):134-139,149.(Yan J C,Wang Y Y,Zhu X F,et al.Study on the biocontrol bacteria inducing soybean cyst nematode and soybean root rot diseases[J].Journal of Anhui Agricaltural Science,2016,44(9):134-139,149.)
[16]米娜瓦尔·艾买提,刘利群.4种种衣剂对大豆根腐病的防效比较[J].安徽农业科学,2013,41(14):6264-6267.(Minawar A,Liu L Q.Comparison of four kinds of seed coating agents on root rot control efficiency of Soybean[J].Journal of Anhui Agricultural Science,2013,41(14):6264-6267.)
[17]朱文静,伍辉军,高学文.芽孢杆菌对大豆根腐病防治效果研究[J].大豆科学,2011,30(4):621-625.(Zhu W J,Wu H J,Gao X W.Control efficacy of Bacillus spp.to soybean root rot[J].Soybean Science,2011,30(4):621-625.)
[18]田大伟,乔俊卿,王伟舵,等.表达Harpin蛋白的芽孢杆菌工程菌防治水稻白叶枯病的研究[J].中国生物防治学报,2012,28(2):250-254.(Tian D W,Qiao J Q,Wang W D,et al.Biocontrol efficacy on rice bacterial leaf blight of genetically engineered Bacillus expressing Harpin[J].Chinese Journal of Biological Control,2012,28(2):250-254.)
[19]Terefe M,Tefera T,Sakhuja P K.Effect of a formulation of Bacillus firmus,on root-knot nematode Meloidogyne incognita,infestation and the growth of tomato plants in the greenhouse and nursery[J].Journal of Invertebrate Pathology,2009,100(2):94-99.
[20]Castillo J D,Lawrence K S,Kloepper J W.Biocontrol of the reniform nematode by Bacillus firmus GB-126 and Paecilomyces lilacinus 251 on cotton[J].Plant Disease,2013,97(7):967-976.
[21]郭丽琢,张虎天,何亚慧,等.根瘤菌接种对豌豆/玉米间作系统作物生长及氮素营养的影响[J].草业学报,2012,21(1):43-49.(Guo L Z,Zhang H T,He Y H,et al.Effect of Rhizobium inoculation on crop growth and nitrogen nutrition of a pea/maize inter cropping system[J].Acta Prataculturae Sinica,2012,21(1):43-49.)
[22]Tian F,Wang Y Y,Zhu X F,et al.Effect of Sinorhizobium fredii strain Sneb183 on the biological control of soybean cyst nematode in soybean[J].Journal of Basic Microbiology,2014,54(11):1258-1263.
[23]么巧君,鲁旭鹏,朱晓峰,等.青霉菌Snef1216发酵液诱导番茄抗南方根结线虫研究[J].植物病理学报,2014,44(6):693-699.(Me Q J,Lu X P,Zhu X F,et al.Resistance against Meloidogyne incognita in tomato induced by fermentation liquid of Penicillium chrysogenum strain Snef1216[J].Acta Phytopathologica Sinica,2014,44(6):693-699.)
[24]王霞,王素英,高朋辉.青霉TS67菌株对大豆根腐病和玉米小斑病的防治效果评价[J].微生物学通报,2008,35(8):1246-1250.(Wang X,Wang S Y,Gao P H.Evaluation of antagonism of Penicillium TS67 against soybean root rot disease and corn southern leaf blight[J].Microbiology,2008,35(8):1246-1250.)
[25]Anastasiadis I A,Giannakou I O,Prophetou-Athanasiadou D A,et al.The combined effect of the application of a biocontrol agent Paecilomyces lilacinus,with various practices for the control of root-knot nematodes[J].Crop Protection,2008,27(3-5):352-361.
[26]刘丽,马鸣超,姜昕,等.根瘤菌与促生菌双接种对大豆生长和土壤酶活的影响[J].植物营养与肥料学报,2015,21(3):644-654.(Liu L,Ma M C,Jiang X,et al.Effect of Rhizobia and PGPR co-inoculant on soybean characteristics and soil enzyme activities[J].Journal of Plant Nutrition and Fertilizer,2015,21(3):644-654.)
[27]丁效东,张林,李淑仪,等.丛枝菌根真菌与根瘤菌接种对大豆根瘤分布及磷素吸收的影响[J].植物营养与肥料学报,2012,18(3):662-669.(Ding X D,Zhang L,Li S Y,et al.Effects of inoculations of Glomus mosseae and/or Bradyrhizobium japonicum on formation and distribution of nodules and phosphorus uptake of soybean[J].Plant Nutrition and Fertilizer Science,2012,18(3):662-669.)
[2]梁喜龙,郑殿峰,左豫虎.大豆根腐病的研究现状及展望[J].黑龙江八一农垦大学学报,2003,15(4):30-34.(Liang X L,Zheng D F,Zuo Y H.The status of the study and prospect on soybean root rot[J].Journal of Heilongjiang August First Land Reclamation University,2003,15(4):30-34.)
[3]段玉玺,陈立杰.大豆胞囊线虫病及其防治[M].北京:金盾出版社,2006:6-7.(Duan Y X,Chen L J.Soybean cyst nematode and its control[M].Beijing:Jindun Publishing House,2006:6-7.)
[4]武亚敬,张金香,高广瑞,等.我国种衣技术的研究进展[J].作物杂志,2007(4):62-66.(Wu J Y,Zhang J X,Gao G R,et al.Research progress on the technology of Chinese seed coating[J].Crops,2007(4):62-66.)
[5]Saikia S K,Tiwari S,Pandey R.Rhizospheric biological weapons for growth enhancement and Meloidogyne incognita,management in Withania somnifera cv.Poshita[J].Biological Control,2013,65(2):225-234.
[6]Akhtar A,Hisamuddin,Robab M I,et al.Plant growth promoting Rhizobacteria:An overview[J].Journal of Natural Product&Plant Resources,2012,2(1):19-31.
[7]郭宁,陈立杰,段玉玺,等.控制大豆胞囊线虫生物种衣剂的研制及沈阳田间小区防效的初步试验[J].植物保护,2009,35(2):158-162.(Guo N,Chen L J,Duan Y X,et al.Preparation and effects of biological seed coating on the soybean cyst nematode in soybean plots in Shenyang[J].Plant Protection,2009,35(2):158-162.)
[8]陈立杰,万传浩,朱晓峰,等.Snea253生物种衣剂防治大豆胞囊线虫的研究[J].大豆科学,2011,30(3):459-462.(Chen L J,Wan C H,Zhu X F,et al.Control effects of Snea253biological seed coating on soybean cyst nematode[J].SoybeanScience,2011,30(3):459-462.)
[9]周园园,王媛媛,朱晓峰,等.生物种衣剂SN101的研制及其对大豆胞囊线虫病的防效[J].中国油料作物学报,2014,36(4):513-518.(Zhou Y Y,Wang Y Y,Zhu X F,et al.Development of a biological seed-coating preparation and it’s efficiency in prevention of Heterodera glycines[J].Chinese Journal of Oil Crop Science,2014,36(4):513-518.)
[10]孙华,段玉玺,焦石,等.抗大豆胞囊线虫的根际促生菌的筛选及其鉴定[J].大豆科学,2009,28(3):507-510.(Sun H,Duan Y X,Jiao S,et al.Filtration and identification of plant growth promoting Rhizobacteria on resistance of soybean cyst nematode[J].Soybean Science,2009,28(3):507-510.)
[11]项鹏,陈立杰,朱晓峰,等.种子处理诱导大豆抗胞囊线虫病的生防细菌筛选与鉴定[J].中国生物防治学报,2013,29(4):661-666.(Xiang P,Chen L J,Zhu X F,et al.Screening and identification of bacterium to induce resistance of soybean against Heterodera glycines[J].Chinese Journal of Biological Control,2013,29(4):661-666.)
[12]田丰,陈立杰,王媛媛,等.根瘤菌Sneb183对大豆胞囊线虫二龄幼虫的作用方式研究[J].中国生物防治学报,2014,30(4):540-545.(Tian F,Chen L J,Wang Y Y,et al.Action mode of Sinorhizobium fredii Sneb183 on the activity of soybean cyst nematode[J],Chinese Journal of Biological Control,2014,30(4):540-545.)
[13]闫继辰,王媛媛,朱晓峰,等.不同真菌发酵液包衣种子对大豆生长和抗病性的影响[J].沈阳农业大学学报,2016,47(4):406-411.(Yan J C,Wang Y Y,Zhu X F,et al.Effect of seed coating with different fungus fermentation liquid on the growth and disease resistance of soybean[J].Journal of Shenyang Agricultural University,2016,47(4):406-411.)
[14]顾鑫,孙力,于铭,等.新型缓释甲霜灵颗粒剂对大豆根腐病主要致病菌的防治效果研究[J].农学学报,2016,30(3):12-15.(Gu X,Sun L,Yu M,et al.Control efficiency of a new type controlled-release metalaxyl granule on main pathogenic bacteria of root rot of soybean[J].Journal of Agriculture,2016,30(3):12-15.)
[15]闫继辰,王媛媛,朱晓峰,等.诱导大豆抗胞囊线虫病和根腐病的生防细菌研究[J].安徽农业科学,2016,44(9):134-139,149.(Yan J C,Wang Y Y,Zhu X F,et al.Study on the biocontrol bacteria inducing soybean cyst nematode and soybean root rot diseases[J].Journal of Anhui Agricaltural Science,2016,44(9):134-139,149.)
[16]米娜瓦尔·艾买提,刘利群.4种种衣剂对大豆根腐病的防效比较[J].安徽农业科学,2013,41(14):6264-6267.(Minawar A,Liu L Q.Comparison of four kinds of seed coating agents on root rot control efficiency of Soybean[J].Journal of Anhui Agricultural Science,2013,41(14):6264-6267.)
[17]朱文静,伍辉军,高学文.芽孢杆菌对大豆根腐病防治效果研究[J].大豆科学,2011,30(4):621-625.(Zhu W J,Wu H J,Gao X W.Control efficacy of Bacillus spp.to soybean root rot[J].Soybean Science,2011,30(4):621-625.)
[18]田大伟,乔俊卿,王伟舵,等.表达Harpin蛋白的芽孢杆菌工程菌防治水稻白叶枯病的研究[J].中国生物防治学报,2012,28(2):250-254.(Tian D W,Qiao J Q,Wang W D,et al.Biocontrol efficacy on rice bacterial leaf blight of genetically engineered Bacillus expressing Harpin[J].Chinese Journal of Biological Control,2012,28(2):250-254.)
[19]Terefe M,Tefera T,Sakhuja P K.Effect of a formulation of Bacillus firmus,on root-knot nematode Meloidogyne incognita,infestation and the growth of tomato plants in the greenhouse and nursery[J].Journal of Invertebrate Pathology,2009,100(2):94-99.
[20]Castillo J D,Lawrence K S,Kloepper J W.Biocontrol of the reniform nematode by Bacillus firmus GB-126 and Paecilomyces lilacinus 251 on cotton[J].Plant Disease,2013,97(7):967-976.
[21]郭丽琢,张虎天,何亚慧,等.根瘤菌接种对豌豆/玉米间作系统作物生长及氮素营养的影响[J].草业学报,2012,21(1):43-49.(Guo L Z,Zhang H T,He Y H,et al.Effect of Rhizobium inoculation on crop growth and nitrogen nutrition of a pea/maize inter cropping system[J].Acta Prataculturae Sinica,2012,21(1):43-49.)
[22]Tian F,Wang Y Y,Zhu X F,et al.Effect of Sinorhizobium fredii strain Sneb183 on the biological control of soybean cyst nematode in soybean[J].Journal of Basic Microbiology,2014,54(11):1258-1263.
[23]么巧君,鲁旭鹏,朱晓峰,等.青霉菌Snef1216发酵液诱导番茄抗南方根结线虫研究[J].植物病理学报,2014,44(6):693-699.(Me Q J,Lu X P,Zhu X F,et al.Resistance against Meloidogyne incognita in tomato induced by fermentation liquid of Penicillium chrysogenum strain Snef1216[J].Acta Phytopathologica Sinica,2014,44(6):693-699.)
[24]王霞,王素英,高朋辉.青霉TS67菌株对大豆根腐病和玉米小斑病的防治效果评价[J].微生物学通报,2008,35(8):1246-1250.(Wang X,Wang S Y,Gao P H.Evaluation of antagonism of Penicillium TS67 against soybean root rot disease and corn southern leaf blight[J].Microbiology,2008,35(8):1246-1250.)
[25]Anastasiadis I A,Giannakou I O,Prophetou-Athanasiadou D A,et al.The combined effect of the application of a biocontrol agent Paecilomyces lilacinus,with various practices for the control of root-knot nematodes[J].Crop Protection,2008,27(3-5):352-361.
[26]刘丽,马鸣超,姜昕,等.根瘤菌与促生菌双接种对大豆生长和土壤酶活的影响[J].植物营养与肥料学报,2015,21(3):644-654.(Liu L,Ma M C,Jiang X,et al.Effect of Rhizobia and PGPR co-inoculant on soybean characteristics and soil enzyme activities[J].Journal of Plant Nutrition and Fertilizer,2015,21(3):644-654.)
[27]丁效东,张林,李淑仪,等.丛枝菌根真菌与根瘤菌接种对大豆根瘤分布及磷素吸收的影响[J].植物营养与肥料学报,2012,18(3):662-669.(Ding X D,Zhang L,Li S Y,et al.Effects of inoculations of Glomus mosseae and/or Bradyrhizobium japonicum on formation and distribution of nodules and phosphorus uptake of soybean[J].Plant Nutrition and Fertilizer Science,2012,18(3):662-669.)