人参皂甙Rg1慢性阻塞性肺疾病大鼠肺组织上皮间充质转化的影响
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摘要
目的通过烟熏法建立慢性阻塞性肺疾病(COPD)大鼠模型,用人参皂甙Rg1灌胃,研究人参皂甙Rg1对小气道上皮细胞上皮间充质转化(EMT)的影响。方法烟熏诱导大鼠COPD模型,观察肺功能变化,肺组织HE染色检测大鼠肺气肿和小气道破坏情况。免疫组织化学、实时PCR检测肺组织E-cad和α-SMA的表达,观察人参皂甙Rg1对肺内EMT的影响。结果人参皂甙Rg1可以减轻烟熏对大鼠肺结构破坏,改善肺功能,降低肺内EMT。结论人参皂甙Rg1可能通过抑制EMT来改善COPD大鼠小气道纤维化。
Objective To investigate the effect and the related mechanism of ginsenoside Rg1 in epithelial mesenchymal transition(EMT) in chronic obstructive pulmonary disease(COPD).Methods The COPD rat models were induced by exposure to cigarette smoke.Ginsenoside Rg1 was oral gavage dosed.The pulmonary function test and lung histologic HE staining were used to evaluate emphysema and small airway destruction.The expressions of E-cad and α-SMA were measured using immunohistochemistry assay,real-time PCR to evaluate the effect of ginsenoside Rg1 on EMT.Results Ginsenoside Rg1 ameliorated emphysema and airway destruction in COPD rats,improved pulmonary function,alleviated airway EMT.Conclusion Ginsenoside Rg1 can protest EMT in lung tissue,and may be act as potent intervention against small airway fibrosis in COPD.
Objective To investigate the effect and the related mechanism of ginsenoside Rg1 in epithelial mesenchymal transition(EMT) in chronic obstructive pulmonary disease(COPD).Methods The COPD rat models were induced by exposure to cigarette smoke.Ginsenoside Rg1 was oral gavage dosed.The pulmonary function test and lung histologic HE staining were used to evaluate emphysema and small airway destruction.The expressions of E-cad and α-SMA were measured using immunohistochemistry assay,real-time PCR to evaluate the effect of ginsenoside Rg1 on EMT.Results Ginsenoside Rg1 ameliorated emphysema and airway destruction in COPD rats,improved pulmonary function,alleviated airway EMT.Conclusion Ginsenoside Rg1 can protest EMT in lung tissue,and may be act as potent intervention against small airway fibrosis in COPD.
引文
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[2]Heijink IH,Brandenburg SM,Postma DS,et al.Cigarette smoke impairs airway epithelial barrier function and cell-cell contact recovery[J].Eur Respir J,2012,39(2):419-428.
[3]Proud D,Hudy MH,Wiehler S,et al.Cigarette smoke modulates expression of human rhinovirus-induced airway epithelial host defense genes[J].PLoS One,2012,7(7):e40762.
[4]Xie XS,Yang M,Liu HC,et al.Ginsenoside Rg1,a major active component isolated from Panax notoginseng,restrains tubular epithelial to myofibroblast transition in vitro[J].J Ethnopharmacol,2009,122(1):35-41.
[5]Yu M,Yu X,Guo D,et al.Ginsenoside Rg1 attenuates invasion and migration by inhibiting transforming growth factor-beta1-induced epithelial to mesenchymal transition in HepG2 cells[J].Mol Med Rep,2015,11(4):3167-3173.
[6]Xie CL,Wang WW,Xue XD,et al.A systematic review and meta-analysis of Ginsenoside-Rg1(G-Rg1)in experimental ischemic stroke[J].Sci Rep-Uk,2015,5:7790.
[7]Yu HT,Zhen J,Pang B,et al.Ginsenoside Rg1 ameliorates oxidative stress and myocardial apoptosis in streptozotocininduced diabetic rats[J].J Zhejiang Uuiv Sci B,2015,16(5):344-354.
[8]Li SS,Ye JM,Deng ZY,et al.Ginsenoside-Rg1 inhibits endoplasmicreticulum stress-inducedapoptosisafter unilateral ureteral obstruction in rats[J].Ren Fail,2015(5),37:890-895.
[9]Wang Y,Liu Y,Zhang XY,et al.Ginsenoside Rg1 regulates innate immune responses in macrophages through differentially modulating the NF-kappaB and PI3K/Akt/mTORpathways[J].Int Immunopharmacol,2014,23(1):77-84.
[10]Xue CC,Shergis JL,Zhang AL,et al.Panax ginseng C.A Meyer root extract for moderate chronic obstructive pulmonary disease(COPD):study protocol for a randomised controlled trial[J].Trials,2011,12(1):164.
[11]An X,Zhang AL,Yang AW,et al.Oral ginseng formulae for stable chronic obstructive pulmonary disease:a systematic review[J].Resp Med,2011,105(2):165-176.
[12]刘乾,刘松,徐卫国,等.泛素特异性蛋白酶19对烟熏诱导慢性阻塞性肺疾病大鼠模型骨骼肌萎缩的作用和机制[J].实验动物与比较医学,2017,37(3):185-190.
[13]Zhao YL,Zhu RT,Sun YL.Epithelial-mesenchymal transition in liver fibrosis[J].Biomed Rep,2016,4(5):269-274.
[14]Bennett J,Cassidy H,Slattery C,et al.Tacrolimus modulates TGF-beta signaling to induce epithelial-mesenchymal transition in human renal proximal tubule epithelial cells[J].J Clin Med,2016,5(5):50.
[15]Zidar N,Bostjancic E,Jerala M,et al.Downregulation of microRNAs of the miR-200 family and up-regulation of Snail and Slug in inflammatory bowel diseases-hallmark of epithelial-mesenchymal transition[J].J Cell Mol Med,2016,20(10):1813-1820.
[16]Ijaz T,Pazdrak K,Kalita M,et al.Systems biology approaches to understanding epithelial mesenchymal transition(EMT)in mucosal remodeling and signaling in asthma[J].World Allergy Organ J,2014,7(1):1-14.
[17]Sun H,Chen J,Qian W,et al.Integrated long non-coding RNA analyses identify novel regulators of epithelial-mesenchymal transition in the mouse model of pulmonary fibrosis[J].J Cell Mol Med,2016,20(7):1234-1246.
[18]Mahmood MQ,Sohal SS,Shukla SD,et al.Epithelial mesenchymal transition in smokers:large versus small airways and relation to airflow obstruction[J].Int J Clin Exp Med,2015,10(1):1515-1524.