柳茶对家兔实验性颈颅动脉粥样硬化的影响
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摘要
目的:研究柳茶对家兔颈、颅动脉粥样硬化(AS)的影响及作用机制。方法:将30只新西兰白色家兔分为5组:正常对照组、AS模型组、阿托伐他汀(3.0 mg·kg~(-1)·d~(-1))组,柳茶高、低剂量(15和7.5g·kg~(-1)·d~(-1))组。采用高脂饲料和免疫损伤法制备AS模型。取颈动脉(carotid artery,CA)做病理检查。颈动脉超声(color doplex flow imaging,CDFI)检测颈动脉内-中膜厚度(intima media thickness,IMT)、血流速度(mean velocity,Vm)、搏动指数(pulsatility index,PI);经颅多普勒超声(transcranial doppler,TCD)检测基底动脉(basilar artery,BA)的Vm和PI。测定家兔血总胆固醇(total cholesterol,TC)、三酰甘油(triglyceride,TG)、低密度脂蛋白(low-density lipoprotein,LDL)、氧化低密度脂蛋白(oxidized low-density lipoprotein,ox-LDL)、丙二醛(malondialdehyde,MDA)、超氧化物歧化酶(suproxide dismutase,SOD)、肿瘤坏死因子(tumor necrosis factor-α,TNF-α)、白介素-6(interleukin-6,IL-6)水平。结果:模型组CA有明显病理性改变;CA和BA的IMT,Vm,PI值升高;血清TC,TG,LDL,ox-LDL,MDA,TNF-α,IL-6水平升高,SOD活性降低,与正常对照组比较有显著性差异(P<0.01)。柳茶2组以上指标均有明显改善,与AS模型组比较有显著性差异(P<0.05,P<0.01)。结论:柳茶具有抗家兔颈、颅AS的作用,机制可能与其调血脂、抗氧化及减少炎性因子有关。
Objective:To study the effects of Liu Tea on experimental atherosclerosis(AS)of carotid artery and cranium in rabbits.Methods:Thirty New Zealand rabbits were divided into five groups:normal control group,AS model group,atorvastatin(3.0 mg·kg~(-1)·d~(-1))group,Liu Tea high-dose(15 g·kg~(-1)·d~(-1))and lowdose(7.5 g·kg~(-1)·d~(-1))groups.AS model was induced by high-fat diet feeding and immune injury.The section of carotid artery(CA)were examined pathologically.The intina-media thickness(IMT),mean velocity(Vm)and pulsatility index(PI)of CA and basilar artery(BA)of rabbits were detected by using CA ultrasonography(CDFI)and transcranial Doppler(TCD).The levels of total cholesterol(TC),triglyceride(TG),low-density lipoprotein(LDL),and contents of oxidized low-density lipoprotein(ox-LDL),malondialdehyde(MDA),tumor necrosis factor-α(TNF-α),interleukin-6(IL-6)and activity of superoxide dismutase(SOD)in the blood rabbits were detected.Results:The CA of the AS model rabbits had significant pathologic changes.The IMT,Vm,PI in the CA and BA,the blood levels of TC,TG,LDL and contents of ox-LDL,MDA,TNF-α,IL-6 were significantly increased,and the activity of SOD was decreased in the AS model group compared with the normal control group(P<0.01).All indices were improved in the Liu Tea groups compared with the AS model group(P<0.05,P<0.01).Conclusion:Liu Tea showed antiatherosclerotic effect on carotid artery and cranium in rabbits.The mechanism involved may be related to that Liu Tea can regulate blood lipid,antioxidation and suppress the production of inflammation factors.
Objective:To study the effects of Liu Tea on experimental atherosclerosis(AS)of carotid artery and cranium in rabbits.Methods:Thirty New Zealand rabbits were divided into five groups:normal control group,AS model group,atorvastatin(3.0 mg·kg~(-1)·d~(-1))group,Liu Tea high-dose(15 g·kg~(-1)·d~(-1))and lowdose(7.5 g·kg~(-1)·d~(-1))groups.AS model was induced by high-fat diet feeding and immune injury.The section of carotid artery(CA)were examined pathologically.The intina-media thickness(IMT),mean velocity(Vm)and pulsatility index(PI)of CA and basilar artery(BA)of rabbits were detected by using CA ultrasonography(CDFI)and transcranial Doppler(TCD).The levels of total cholesterol(TC),triglyceride(TG),low-density lipoprotein(LDL),and contents of oxidized low-density lipoprotein(ox-LDL),malondialdehyde(MDA),tumor necrosis factor-α(TNF-α),interleukin-6(IL-6)and activity of superoxide dismutase(SOD)in the blood rabbits were detected.Results:The CA of the AS model rabbits had significant pathologic changes.The IMT,Vm,PI in the CA and BA,the blood levels of TC,TG,LDL and contents of ox-LDL,MDA,TNF-α,IL-6 were significantly increased,and the activity of SOD was decreased in the AS model group compared with the normal control group(P<0.01).All indices were improved in the Liu Tea groups compared with the AS model group(P<0.05,P<0.01).Conclusion:Liu Tea showed antiatherosclerotic effect on carotid artery and cranium in rabbits.The mechanism involved may be related to that Liu Tea can regulate blood lipid,antioxidation and suppress the production of inflammation factors.
引文
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[13]武燕,刘健.藏药柳茶挥发油对小鼠急性心肌缺血损伤的干预作用[J].中国现代应用药学,2017,34(3):315-318.
[2]王昕,马慧萍.藏药柳茶分子进化特征与主要成分研究概况[J].中国民族民间医药,2017,26(16):56-58.
[3]谢健,魏丹,沈超,等.活血通脉颗粒对兔颈动脉粥样硬化干预作用的实验研究[J].中国中医急症,2016,25(12):2249-2252.
[4]黄德章,李壮志,张学军,等.选择性椎-基底动脉造影和经颅多普勒对兔蛛网膜下腔出血脑血管痉挛的评价[J].泰山医学院学报,2013,34(6):423-426.
[5]华扬.颈动脉超声临床研究与应用进展[J].中华医学超声杂志,2015,15(4):256-259.
[6]邢英琦.颅脑与颈动脉超声诊断模板与图谱[M].北京:人民卫生出版社,2017:32-39.
[7]王进,朱萱萱,于云华,等.荨芍血脉平胶囊对高脂家兔血脂水平和动脉硬化病变进展的抑制作用[J].中华中医药学刊,2015,33(8):32-35.
[8]钟伟,王永刚,于远望,等.中医药防治动脉粥样硬化的作用机制研究进展[J].时珍国医国药,2017,28(1):190-192.
[9]陈文,王沛明,曾勇,等.基于TLR4通路初探黄芩对内毒素血症大鼠肝肺组织中细胞因子IL-1β、IL-6、TNF-α以及氧化应激因子MDA、SOD水平的影响[J].中药药理与临床,2016,32(3):87-92.
[10]彭晶.SOD、NO、TNF-α和hs-CRP对高血压合并糖尿病患者动脉粥样硬化的影响.[J].国际检验医学杂志,2016,37(23):3285-3287.
[11]HAROKOPAKIS E,HAJISHNGALLIS G.Integrin activation by bacterial fimbriae through a pathway involving CD14,Toll-like receptor-2,and phosphatidylinisitol-3-kinase[J].Eur J Immunol,2005,35(4):1201-1210.
[12]尹玉洁,马柳一,贾振华,等.“营卫通络方”对动脉粥样硬化早期管壁微血管新生的作用[J].中国新药杂志,2016,25(11):1281-1285.
[13]武燕,刘健.藏药柳茶挥发油对小鼠急性心肌缺血损伤的干预作用[J].中国现代应用药学,2017,34(3):315-318.