HPLC法同时测定9个不同产区人参花中5种皂苷含量
|
摘要
目的:建立HPLC法测定人参花中5种人参皂苷Re、Rb_1、Rg_2、Rb_2、Rd的含量。方法:采用Thermo HYPERSIL GOLD C_(18)(250 mm×4.6 mm,5μm)色谱柱;以乙腈-0.1%磷酸-水为流动相,梯度洗脱;流速:0.8 mL/min;柱温:30℃;检测波长:203 nm;进样量:10μL。结果:人参皂苷Re在0.987～12.325μg范围内线性关系良好,平均加样回收率为99.75%,RSD%为2.23%;人参皂苷Rb_1在1.999～24.975μg范围内线性关系良好,平均加样回收率为99.11%,RSD%为0.155%;人参皂苷Rg2在2.040～25.500μg范围内线性关系良好,平均加样回收率为99.32%,RSD%为1.37%;人参皂苷Rb_2在6.210～77.625μg范围内线性关系良好,平均加样回收率为101.27%,RSD%为0.82%;人参皂苷Rd在5.560～69.500μg范围内线性关系良好,平均加样回收率为99.72%,RSD%为1.95%。结论:该方法可用于人参花中人参皂苷Re、Rb_1、Rg_2、Rb_2、Rd的含量测定。
Objective: To establish an HPLC method to test the concentration of five different ginsenosides(Re, Rb1, Rg2, Rb2 and Rd) in flower buds of Panax ginseng C.A. Meyer. Methods: HPLC was performed with Thermo HYPERSIL GOLD C18(250 mm×4.6 mm, 5 μm); with a mobile phase of flow rate 0.8 mL/min; the column temperature was 30 ℃; the detection wavelength was at 203 nm,the injection volume was 10 mL. Results: The linear relationship of Re was good in the range of 0.987～12.325 μg, with an average recovery of 99.75% and RSD% of 2.23%. The linear relationship of Rb_1 was good in the range of 1.999～24.975 μg, with an average recovery of 99.11% and RSD% of 0.155%. The linear relationship of Rg_2 was good in the range of 2.040～25.500 μg,with an average recovery of 99.32% and RSD% of 1.37%. The linear relationship of Rb_2 was good in the range of 6.210～77.625 μg, the average recovery of sample addition was 101.27% and RSD% was 0.82%. The linear relationship of Rd was good in the range of 5.560 ～69.500 μg, the average recovery of sample addition was99.72% and RSD% was 1.95%. Conclusion: The method can be used for the determination of Re, Rb_1, Rg_2,Rb_2 and Rd in the flower Buds of Panax ginseng C.A.Meyer.
Objective: To establish an HPLC method to test the concentration of five different ginsenosides(Re, Rb1, Rg2, Rb2 and Rd) in flower buds of Panax ginseng C.A. Meyer. Methods: HPLC was performed with Thermo HYPERSIL GOLD C18(250 mm×4.6 mm, 5 μm); with a mobile phase of flow rate 0.8 mL/min; the column temperature was 30 ℃; the detection wavelength was at 203 nm,the injection volume was 10 mL. Results: The linear relationship of Re was good in the range of 0.987～12.325 μg, with an average recovery of 99.75% and RSD% of 2.23%. The linear relationship of Rb_1 was good in the range of 1.999～24.975 μg, with an average recovery of 99.11% and RSD% of 0.155%. The linear relationship of Rg_2 was good in the range of 2.040～25.500 μg,with an average recovery of 99.32% and RSD% of 1.37%. The linear relationship of Rb_2 was good in the range of 6.210～77.625 μg, the average recovery of sample addition was 101.27% and RSD% was 0.82%. The linear relationship of Rd was good in the range of 5.560 ～69.500 μg, the average recovery of sample addition was99.72% and RSD% was 1.95%. Conclusion: The method can be used for the determination of Re, Rb_1, Rg_2,Rb_2 and Rd in the flower Buds of Panax ginseng C.A.Meyer.
引文
[1]安镁,叶柏.叶柏运用厚朴生姜半夏甘草人参汤经验举隅[J].中医药导报,2016,22(1):116.
[2]王海南.人参皂苷药理研究进展[J].中国临床药理学与治疗学,2006,11(11):1201-1206.
[3]高明菊,崔秀明,曾江,等.三七花的研究进展[J].人参研究,2009,6(2):6.
[4]王肖,尤昭玲.浅析尤昭玲教授妇科临证巧用三七花、人参花的经验[J].中医药导报,2013,9(19):16-17.
[5]田成刚.珍贵的人参花蕾[J].科学时代,2012(5):334-335.
[6]潘小玲,李振麟,钱士辉,等.中国人参花化学成分研究[J].中国野生植物资源,2014,33(2):13-15.
[7]潘小玲.人参花的化学成分研究[D].南京:中国药科大学,2014.
[8]朱艳琴,殷勤红,虞泓,等.HPLC法同时测定三七花中3种皂苷[J].中成药,2013,2(35):339-341.
[9]张伟云,陈全成,郑毅男,等.RP-HPLC测定人参个部位人参皂苷compound K的含量[J].药物分析杂志,2007,27(1):1-3.
[10] Sung Kwon Ko, Ok Sun Cho, Hye Min Bae, et al.Quantitative analysis of Ginsenosides composition in Flower Buds of Various Ginseng Plants[J].Journal of the Korean Society for Applied Biological Chemistry,2011,54(1):154-157.
[11]陈斌,赵伯涛,钱骅,等.人参花超微粉碎扫描电镜观察及人参皂苷测定[J].中成药,2012,10(34):1974-1978.
[12] Xin Jin, Ling-Ying Zhu, Hong Shen, et al.Influence of sulphur-fumigation on the quality of white ginseng:A quantitative evaluation of major ginsenosides by high performance liquid chromatography[J].Food Chemistry,2012(135):1141-1147.
[13]卓丹如,钱丽萍,章宁,等.HPLC指纹图谱法测定不同粉碎度野山花旗参粉末中人参皂苷的含量[J].中国执业药师,2014,8(1):20-23.
[2]王海南.人参皂苷药理研究进展[J].中国临床药理学与治疗学,2006,11(11):1201-1206.
[3]高明菊,崔秀明,曾江,等.三七花的研究进展[J].人参研究,2009,6(2):6.
[4]王肖,尤昭玲.浅析尤昭玲教授妇科临证巧用三七花、人参花的经验[J].中医药导报,2013,9(19):16-17.
[5]田成刚.珍贵的人参花蕾[J].科学时代,2012(5):334-335.
[6]潘小玲,李振麟,钱士辉,等.中国人参花化学成分研究[J].中国野生植物资源,2014,33(2):13-15.
[7]潘小玲.人参花的化学成分研究[D].南京:中国药科大学,2014.
[8]朱艳琴,殷勤红,虞泓,等.HPLC法同时测定三七花中3种皂苷[J].中成药,2013,2(35):339-341.
[9]张伟云,陈全成,郑毅男,等.RP-HPLC测定人参个部位人参皂苷compound K的含量[J].药物分析杂志,2007,27(1):1-3.
[10] Sung Kwon Ko, Ok Sun Cho, Hye Min Bae, et al.Quantitative analysis of Ginsenosides composition in Flower Buds of Various Ginseng Plants[J].Journal of the Korean Society for Applied Biological Chemistry,2011,54(1):154-157.
[11]陈斌,赵伯涛,钱骅,等.人参花超微粉碎扫描电镜观察及人参皂苷测定[J].中成药,2012,10(34):1974-1978.
[12] Xin Jin, Ling-Ying Zhu, Hong Shen, et al.Influence of sulphur-fumigation on the quality of white ginseng:A quantitative evaluation of major ginsenosides by high performance liquid chromatography[J].Food Chemistry,2012(135):1141-1147.
[13]卓丹如,钱丽萍,章宁,等.HPLC指纹图谱法测定不同粉碎度野山花旗参粉末中人参皂苷的含量[J].中国执业药师,2014,8(1):20-23.