石柱和抚松产人参叶指纹图谱分析
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摘要
[目的]建立石柱和抚松人参叶药材的高效液相色谱(HPLC)指纹图谱,以探讨两产地人参叶皂苷含量的差异。[方法]采用HPLC法分别测定采于辽宁宽甸石柱、吉林抚松的人参叶HPLC指纹图谱,运用中药色谱指纹图谱相似度评价系统对各样品进行分析。[结果]10批人参叶样品的HPLC指纹图谱相似度均大于0.9,标定了22个共有峰,不同产地人参叶共有峰相对含量存在一定的差异。[结论]建立的人参叶HPLC指纹图谱分析方法科学可靠,可为人参叶药材质量研究提供参考。
[Objective] To study the difference of ginsenoside content from different production places by establishing HPLC fingerprints of ginseng leaf. [Methods] HPLC fingerprints of ginseng leaf samples from Liaoning Province, Dandong City, Kuandian Manchu Autonomous County Township Shizhu Village and Jilin Province, Fusong County were analyzed by HPLC. The similarity of each sample was analyzed by similarity evaluation system for chromatographic fingerprint of chinese materia media. [Results] The chromatographic fingerprint similarity of ten batches of ginseng leaf samples is more than 0.9 and there are altogether 22 common peaks, but has some differences between the relative area of the common peaks. [Conclusion] HPLC fingerprints of ginseng leaf from different production places will provide a reference standard for the study of ginseng leaf from different production places.
[Objective] To study the difference of ginsenoside content from different production places by establishing HPLC fingerprints of ginseng leaf. [Methods] HPLC fingerprints of ginseng leaf samples from Liaoning Province, Dandong City, Kuandian Manchu Autonomous County Township Shizhu Village and Jilin Province, Fusong County were analyzed by HPLC. The similarity of each sample was analyzed by similarity evaluation system for chromatographic fingerprint of chinese materia media. [Results] The chromatographic fingerprint similarity of ten batches of ginseng leaf samples is more than 0.9 and there are altogether 22 common peaks, but has some differences between the relative area of the common peaks. [Conclusion] HPLC fingerprints of ginseng leaf from different production places will provide a reference standard for the study of ginseng leaf from different production places.
引文
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[5]李连昆,程俊霖,赵妍妍,等.人参茎叶总皂苷对衰老皮肤胶原纤维和弹力纤维的保护作用[J].四川生理科学杂志,2007,29(1):26-28.
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[11]刘菲,赵文杰,刘南,等.人参茎叶皂苷对毛囊间充质干细胞增殖的影响[J].黑龙江医药,2012,25(2):232-234.
[12]程俊霖,周黎明,朱玲,等.人参茎叶总皂苷对衰老小鼠的作用研究[J].四川生理科学杂志,2004,26(3):97-99.
[13]马小琼,王龙星,徐青,等.人参皂甙的反相高效液相色谱多台阶梯度优化方法[J].高等学校化学学报,2004,25(2):238-242.
[2]国家药典委员会.中华人民共和国药典(一部)[S].北京:中国医药科技出版社,2015:9-10.
[3]Park EK,Choo MK,Kim EJ,et al.Antiallergic activity of ginseoside Rh2[J].Biological Pharmaceutical Bulletin,2003,26(11):1581-1584.
[4]张晓文,宋清,徐志伟.人参茎叶总皂苷对心理应激荷瘤小鼠肿瘤生长及化疗效果的影响[J].现代食品科技,2010,26(4):348-350.
[5]李连昆,程俊霖,赵妍妍,等.人参茎叶总皂苷对衰老皮肤胶原纤维和弹力纤维的保护作用[J].四川生理科学杂志,2007,29(1):26-28.
[6]程秀娟.人参、人参茎叶皂甙和参杞安泰抗衰老作用的比较研究[J].老年学杂志,1992,12(1):52-54.
[7]王秋静,刘芬,崔新明,等.人参茎叶三醇皂苷对心肌缺血大鼠心肌酶及超微结构的影响[J].中成药,2011,33(2):2153-2154.
[8]娄建石,白凤菊,田秀英,等.人参茎叶皂甙对大鼠心功能的作用研究[J].天津医科大学学报,2001,7(4):488-491.
[9]胡巢凤,陆大祥,孙丽萍,等.人参茎叶皂苷对小鼠脂肪肝的作用及机制研究[J].中国药理学通报,2009,25(5):663-667.
[10]马肃,王北辰,吕俊华,等.人参茎叶皂甙对小鼠免疫功能的影响[J].中国病理生理杂志,1991,7(2):126-128.
[11]刘菲,赵文杰,刘南,等.人参茎叶皂苷对毛囊间充质干细胞增殖的影响[J].黑龙江医药,2012,25(2):232-234.
[12]程俊霖,周黎明,朱玲,等.人参茎叶总皂苷对衰老小鼠的作用研究[J].四川生理科学杂志,2004,26(3):97-99.
[13]马小琼,王龙星,徐青,等.人参皂甙的反相高效液相色谱多台阶梯度优化方法[J].高等学校化学学报,2004,25(2):238-242.