抗人SDF-1多克隆抗体对糖尿病大鼠视网膜血管内皮生长因子表达的影响
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摘要
目的:研究抗人基质细胞衍生因子(SDF-1)多克隆抗体对糖尿病大鼠(DM)视网膜血管内皮生长因子(VEGF)表达的影响,为抗SDF-1多克隆抗体用于防治糖尿病视网膜病变(Diabetic retinopa-thy,DR)提供实验依据。方法:采用链脲佐菌素腹腔注射,建立DM动物模型。治疗组,用抗人SDF-1多克隆抗体治疗,对照组和非治疗组只给予等量生理盐水。结果:对照组大鼠视网膜VEGF的含量为(4.25±0.81)ng/ml,未治疗组大鼠视网膜VEGF的含量为(13.32±1.12)ng/ml,两组比较差异有统计学意义(P<0.01);治疗组大鼠视网膜VEGF的含量为(6.73±1.25)ng/ml,同未治疗组比较,差异有统计学意义(P<0.01)。结论:抗人SDF-1多克隆抗体抑制糖尿病大鼠视网膜血管内皮生长因子的表达。
Objective: To study the effect of anti-human stromal cell derived factor(SDF-1) polyclonal antibody on rat diabetic retinal(DM)vascular endothelial growth factor(VEGF) expression,to provide experimental basis for anti SDF-1 polyclonal antibody to prevent and treat diabetic retinopathy(DR).Methods: By intraperitoneal injection of streptozotocin to establish DM animal model.The treatment group was given the anti-human SDF-1 polyclonal antibody therapy.The control group and untreated group were given saline.Results: In non-treatment group,the VEGF level in retina of rats with diabetes was 13.32±1.12 ng/ml,significantly higher than the normal control group(4.25±0.81 ng/ml)(P<0.01) and the treatment group(6.73±1.25 ng/ml)(P<0.01).Conclusion: Anti-human SDF-1 polyclonal antibody can inhibit the expression of VEGF in diabetic rat retina.
Objective: To study the effect of anti-human stromal cell derived factor(SDF-1) polyclonal antibody on rat diabetic retinal(DM)vascular endothelial growth factor(VEGF) expression,to provide experimental basis for anti SDF-1 polyclonal antibody to prevent and treat diabetic retinopathy(DR).Methods: By intraperitoneal injection of streptozotocin to establish DM animal model.The treatment group was given the anti-human SDF-1 polyclonal antibody therapy.The control group and untreated group were given saline.Results: In non-treatment group,the VEGF level in retina of rats with diabetes was 13.32±1.12 ng/ml,significantly higher than the normal control group(4.25±0.81 ng/ml)(P<0.01) and the treatment group(6.73±1.25 ng/ml)(P<0.01).Conclusion: Anti-human SDF-1 polyclonal antibody can inhibit the expression of VEGF in diabetic rat retina.
引文
[1]Grant M B,Afzal A,Spoerri P,et al.The role of growthfactors in the pathogenesis of diabetic retinopathy[J].Ex-pert Opine Investing Drugs,2004,13(10):1275-1293.
[2]袁源智,袁非.实验性糖尿病对视网膜基质细胞衍生因子1表达的影响[J].中华眼科杂志,2007,43(10):912-916.
[3]Asahara T,Murohara T,Sullivan A,et al.Isolation of pu-tative progenitor endothelial cells for angiogenesis[J].Sci-ence,1997,275:964-967.
[4]Simons M.Angiogenesis:Where do we stand now[J].Cir-culation,2005,111(12):1556-1566.
[5]Grant M B,Afzal A,Spoerri P,et al.The role of growthfactors in the pathogenesis of diabetic retinopathy[J].Ex-pert Opin Investig Drugs,2004,13(10):1275-1293.
[6]蒋晓萌,唐宁,张奕霞,等.早期糖尿病大鼠视网膜中血管生成素-1、血管内皮生长因子的表达[J].现代生物医学进展,2010,10(2):263-266.
[7]Salcedo R,Oppenheim J J.Role of chemokines in angio-genesis:CXCL12/SDF-1and CXCR4interaction,a keyregulator of endothelial cell responses[J].Microcircula-tion,2003,10:359-370.
[8]游逸安,许雯怡,朱东如,等.增殖性糖尿病视网膜病变玻璃体与血清SDF-1、VEGF含量分析[J].医学研究杂志,2013,42(1):100-104.
[9]林安华,雷闽湘,谢晓芸,等.糖尿病大鼠视网膜病变中促血管生成因子的表达及意义[J].中南大学学报:医学版,2009,34(12):1243-1250.
[2]袁源智,袁非.实验性糖尿病对视网膜基质细胞衍生因子1表达的影响[J].中华眼科杂志,2007,43(10):912-916.
[3]Asahara T,Murohara T,Sullivan A,et al.Isolation of pu-tative progenitor endothelial cells for angiogenesis[J].Sci-ence,1997,275:964-967.
[4]Simons M.Angiogenesis:Where do we stand now[J].Cir-culation,2005,111(12):1556-1566.
[5]Grant M B,Afzal A,Spoerri P,et al.The role of growthfactors in the pathogenesis of diabetic retinopathy[J].Ex-pert Opin Investig Drugs,2004,13(10):1275-1293.
[6]蒋晓萌,唐宁,张奕霞,等.早期糖尿病大鼠视网膜中血管生成素-1、血管内皮生长因子的表达[J].现代生物医学进展,2010,10(2):263-266.
[7]Salcedo R,Oppenheim J J.Role of chemokines in angio-genesis:CXCL12/SDF-1and CXCR4interaction,a keyregulator of endothelial cell responses[J].Microcircula-tion,2003,10:359-370.
[8]游逸安,许雯怡,朱东如,等.增殖性糖尿病视网膜病变玻璃体与血清SDF-1、VEGF含量分析[J].医学研究杂志,2013,42(1):100-104.
[9]林安华,雷闽湘,谢晓芸,等.糖尿病大鼠视网膜病变中促血管生成因子的表达及意义[J].中南大学学报:医学版,2009,34(12):1243-1250.