智能型水凝胶用于皮肤烧伤治疗的研究进展
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摘要
缓慢的伤口愈合、感染以及增生性瘢痕是皮肤烧伤治疗领域的难点和热点,慢性伤口感染是烧伤患者的主要并发症,也是疼痛的主要原因之一。水凝胶作为一种3D网状交联高分子聚合物,具有良好的塑形性和水溶性,可以防止体液流失,保持伤口表面水化,促进愈合过程,还可作为防止细菌感染的屏障。目前水凝胶已被广泛用于促进伤口愈合,尤其是烧伤伤口。相较于普通水凝胶,新型智能型水凝胶因具有细胞黏附能力、高度生物相容性、无毒及环保等优点,且对各种生理或外部刺激具有特异的响应,而在烧伤治疗领域具有更广阔的应用前景。
Slow wound healing,infection and proliferative scar are difficult points and hot spots in the field of skin burn treatment. Chronic wound infection is still the main cause of complications and severe pain in burn patients. As a kind of polymer with three-dimensional reticulated cross-linking structure,hydrogel has good plasticity and water solubility. It can prevent the loss of body fluid,keep the wound surface hydrated,promote healing process,and act as a barrier to prevent bacterial infection. Hydrogel has been widely used to promote wound healing process,especially burn wound. Compared with ordinary hydrogels,the new intelligent hydrogels have the advantages of cell adhesion,high biocompatibility,non-toxicity and environmental protection,and have specific responses to various physiological or external stimuli,which allow broader application prospects in the field of burn treatment.
Slow wound healing,infection and proliferative scar are difficult points and hot spots in the field of skin burn treatment. Chronic wound infection is still the main cause of complications and severe pain in burn patients. As a kind of polymer with three-dimensional reticulated cross-linking structure,hydrogel has good plasticity and water solubility. It can prevent the loss of body fluid,keep the wound surface hydrated,promote healing process,and act as a barrier to prevent bacterial infection. Hydrogel has been widely used to promote wound healing process,especially burn wound. Compared with ordinary hydrogels,the new intelligent hydrogels have the advantages of cell adhesion,high biocompatibility,non-toxicity and environmental protection,and have specific responses to various physiological or external stimuli,which allow broader application prospects in the field of burn treatment.
引文
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[2]Wang Y,Beekman J,Hew J,et al.Burn injury:Challenges and advances in burn wound healing,infection,pain and scarring[J].Adv Drug Deliv Rev,2018,123:3-17.
[3]Peck MD.Epidemiology of burns throughout the world.Part I:Distribution and risk factors[J].Burns,2011,37(7):1087-1100.
[4]Buwalda SJ,Boere KW,Dijkstra PJ,et al.Hydrogels in a historical perspective:From simple networks to smart materials[J].J Control Release,2014,190:254-273.
[5]陈亮,姚峰.载细胞水凝胶在椎间盘修复中的应用[J].医学综述,2017,23(18):3651-3655.
[6]Drury JL,Mooney DJ.Hydrogels for tissue engineering:Scaffold design variables and applications[J].Biomaterials,2003,24(24):4337-4351.
[7]Archana D,Dutta J,Dutta PK.Evaluation of chitosan nano dressing for wound healing:Characterization,in vitro and in vivo studies[J].Int J Biol Macromo,2013,57:193-203.
[8]Ferreira MOG,Leite LLR,de Lima IS,et al.Chitosan hydrogel in combination with Nerolidol for healing wounds[J].Carbohydr Polym,2016,152:409-418.
[9]Nie J,Lu W,Ma J,et al.Orientation in multi-layer chitosan hydrogel:Morphology,mechanism,and design principle[J].Sci Rep,2015,5:7635.
[10]Miguel SP,Ribeiro MP,Brancal H,et al.Thermoresponsive chitosanagarose hydrogel for skin regeneration[J].Carbohydr Polym,2014,111:366-373.
[11]El-Kased RF,Amer RI,Attia D,et al.Honey-based hydrogel:In vitro and comparative In vivo evaluation for burn wound healing[J].Sci Rep,2017,7(1):9692.
[12]Zhai M,Xu Y,Zhou B,et al.Keratin-chitosan/n-ZnO nanocomposite hydrogel for antimicrobial treatment of burn wound healing:Characterization and biomedical application[J].J Photochem Photobiol B,2018,180:253-258.
[13]Ouyang QQ,Hu Z,Lin ZP,et al.Chitosan hydrogel in combination with marine peptides from tilapia for burning healing[J].In JBiol Macromol,2018,112:1191-1198.
[14]Sun G,Zhang X,Shen YI.Dextran hydrogel scaffolds enhance angiogenic responses and promote complete skin regeneration during burn wound healing[J].Proc Nati Acad Sci U S A,2011,108(52):20976-20981.
[15]Shen YI,Song HG,Papa A,et al.Acellular hydrogels for regenerative burn wound healing:Translation from a porcine model[J].J Invest Dermatol,2015,135(10):2519-2529.
[16]Greaves NS,Iqbal SA,Baguneid M,et al.The role of skin substitutes in the management of chronic cutaneous wounds[J].Wound Repair Regen,2013,21(2):194-210.
[17]Nicholas MN,Jeschke MG,Amini-Nik S.Cellularized bilayer pullulan-gelatin hydrogel for skin regeneration[J].Tissue Eng Part A,2016,22(9/10):754-764.
[18]Ratner BD,Hoffman AS.Synthetic hydrogels for biomedical applications[J].Adv Drug Deliv Rev,1976,54(1):3-12.
[19]Kim MH,Park H,Nam HC,et al.Injectable methylcellulose hydrogel containing silver oxide nanoparticles for burn wound healing[J].Carbohydr Polym,2018,181:579-586.
[20]Zaborowska M,Bodin A,Bckdahl H,et al.Microporous bacterial cellulose as a potential scaffold for bone regeneration[J].Acta Biomater,2010,6(7):2540-2547.
[21]Lin WC,Lien CC,Yeh HJ,et al.Bacterial cellulose and bacterial cellulose-chitosan membranes for wound dressing applications[J].Carbohydr Polym,2013,94(1):603-611.
[22]Kwak MH,Kim JE,Go J,et al.Bacterial cellulose membrane produced by Acetobacter sp.A10 for burn wound dressing applications[J].Carbohydr Polym,2015,122:387-398.
[23]Pandey M,Mohamad N,Low WL,et al.Microwaved bacterial cellulose-based hydrogel microparticles for the healing of partial thickness burn wounds[J].Drug Deliv Transl Res,2017,7(1):88-89.
[24]Mohamad N,Mohd Amin MC,Pandey M,et al.Bacterial cellulose/acrylic acid hydrogel synthesized via electron beam irradiation:Accelerated burn wound healing in an animal model[J].Carbohydr Polym,2014,114:312-320.
[25]Mohamad N,Loh EYX,Fauzi MB,et al.In vivo evaluation of bacterial cellulose/acrylic acid wound dressing hydrogel containing keratinocytes and fibroblasts for burn wounds[J].Drug Deliv Transl Res,2018(6):1-9.
[26]Loh EYX,Mohamad N,Fauzi MB,et al.Development of a bacterial cellulose-based hydrogel cell carrier containing keratinocytes and fibroblasts for full-thickness wound healing[J].Sci Rep,2018,8(1):2875.
[27]Nalampang K,Panjakha R,Molloy R,et al.Structural effects in photopolymerized sodium AMPS hydrogels crosslinked with poly(ethylene glycol)diacrylate for use as burn dressings[J].J Biomater Sci Polym Ed,2013,24(11):1291-1304.
[28]Wang H,Cheng F,He W,et al.Poly(ethylene)glycol hydrogel based on oxa-Michael reaction:Precursor synthesis and hydrogel formation[J].Biointerphases,2017,12(2):02C414.
[29]Campos EVR,Oliveira JL,Fraceto LF.Poly(ethylene glycol)and cyclodextrin-grafted chitosan:From methodologies to preparation and potential biotechnological applications[J].Front Chem,2017,5:93.
[30]Yan Q,Xiao LQ,Tan L,et al.Controlled release of simvastatinloaded thermo-sensitive PLGA-PEG-PLGA hydrogel for bone tissue regeneration:In vitro and in vivo characteristics[J].J Biomed Mater Res A,2015,103(11):3580-3589.
[31]Burmeister DM,Stone R 2nd,Wrice N,et al.Delivery of allogeneic adipose stem cells in polyethylene glycol-fibrin hydrogels as an adjunct to meshed autografts after sharp debridement of deep partial thickness burns[J].Stem Cells Transl Med,2018,7(4):360-372.
[32]Konieczynska MD,Villa-Camacho JC,Ghobril C,et al.On-demand dissolution of a dendritic hydrogel-based dressing for second-degree burn wounds through thiol-thioester exchange reaction[J].Angew Chem Int Ed Engl,2016,55(34):9984-9987.
[33]Wu J,Xiao Z,Chen A,et al.Sulfated zwitterionic poly(sulfobetaine methacrylate)hydrogels promote complete skin regeneration[J].Acta Biomater,2018,71:293-305.