炒莱菔子水提液中萝卜苷在人工胃肠液中的稳定性研究
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摘要
目的:考察炒莱菔子水提取液中的萝卜苷在人工胃肠液中的稳定性。方法:采用高效液相色谱测定莱菔子水提液中萝卜苷在人工胃液NE(未加胃蛋白酶)、人工胃液和人工肠液NE(未加胰酶)、人工肠液中不同时间的峰面积,计算各自质量浓度及平均降解百分率。结果:萝卜苷在人工胃肠液中均较稳定,通过单因素方差分析各时间点降解百分率与0 h比较均无显著性差异(P>0.05)。结论:萝卜苷在人工胃肠液中稳定性良好。
Objective:To investigate the stability of glucoraphenin in artificial gastic and intestinal juice from water extractof roasted Raphani Semen.Method:The area of glucoraphenin at different time points in artificial gastic fluid with or without pepsin and artificial intestinal fluid with or without pancreatin was determined by HPLC,and the mass concentration and average remaining percentage were calculated respectively. Result: Glucorapheninwas stable in artificial gastric andintestinal juice. The degradation percentage did not have significant difference between 0 hour and each time point by One-way analysis of variance. Conclusion: Glucoraphenin was stable both in artificial gastric juice and in intestinal juice,no matter whether the relative enzyme was added in.
Objective:To investigate the stability of glucoraphenin in artificial gastic and intestinal juice from water extractof roasted Raphani Semen.Method:The area of glucoraphenin at different time points in artificial gastic fluid with or without pepsin and artificial intestinal fluid with or without pancreatin was determined by HPLC,and the mass concentration and average remaining percentage were calculated respectively. Result: Glucorapheninwas stable in artificial gastric andintestinal juice. The degradation percentage did not have significant difference between 0 hour and each time point by One-way analysis of variance. Conclusion: Glucoraphenin was stable both in artificial gastric juice and in intestinal juice,no matter whether the relative enzyme was added in.
引文
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[18] 任涛,吕文海. 以硫代葡萄糖苷相对含量优选莱菔子炮制工艺研究[J].中成药,2010,7:1159-1162.
[19] 毛茜,傅超美,胡慧玲,等. 川木香煨制前后主要药效成分在人工胃、肠液及大鼠离体胃肠共孵液中的稳定性研究[J]. 中国中药杂志,2012,6:785-789.
[20] 国家药典委员会.中华人民共和国药典:四部[S].北京:中国医药科技出版社,2015:119.
[21] 盛华刚,朱立俏,张津萌.金荞麦原花青素B2和表儿茶素在人工胃肠液中的含量变化[J].中国实验方剂学杂志,2014,20(4):77-80.
[22] 陈邦姣,朱立俏,孔得刚,等. 莱菔子中芥子碱硫氰酸盐在人工胃肠液中的稳定性研究[J]. 食品与药品,2014,3:188-190.
[2] 朱立俏,盛华刚,周洪雷.基于指纹图谱考察不同型号大孔树脂对莱菔子水提液的纯化效果[J].中国实验方剂学杂志,2013,19(24):21-24.
[3] Yunlun Li,Wenqing Yang,Qingjun Zhu,et al.Protective effects on vascular endothelial cell in N’-nitro-L-arginine(L-NNA)-induced hypertensive rats from the combination of effective components of Uncaria rhynchophylla and Semen Raphani[J].BioScience Trends,2015,9(4):237-244.
[4] 杨金果, 李运伦,周洪雷.钩藤和莱菔子生物碱抗高血压血管内皮细胞损伤效应[J]. 中成药, 2013,35(5):889-893.
[5] 张国侠,盖国忠.莱菔子总生物碱对Apo E基因敲除小鼠血脂的影响[J].中国老年学杂志, 2010, 30(6): 844-845.
[6] Hui Q Zhang,Shi Y Chen,An S Wang,et al. Sulforaphane induces adipocyte browning and promotes glucose and lipid utilization[J]. Molecular Nutrition & Food Research,2016,60(10):2185-2197.
[7] 谭鹏,薛玲,吕文海.莱菔子不同炮制品对呼吸系统作用的实验研究[J]. 山东中医杂志, 2005,24(5): 300-302.
[8] Patricia A Egner,Jianguo Chen,Jinbing Wang,et al.Bioavailability of sulforaphane from two broccoli sprout beverages:Results of a short term, cross-over clinical trial in Qidong, China[J]. Cancer Prevention Research (Philadelphia, Pa.), 2011,4(3):384-395.
[9] Jeries Jadoun,Ahmad Yazbak,Salwa Rushrush,et al. Identification of a New Antibacterial Sulfur Compound from Raphanus sativus Seeds[J]. Evidence-Based Complementary and Alternative Medicine,2016,2016:9271285.
[10] Kendra J Royston,Trygve O Tollefsbol. The epigenetic impact of cruciferous vegetables on cancer prevention[J]. Current Pharmacology Reports, 2015,1(1):46-51.
[11] 唐健元,张磊,彭成. 莱菔子行气消食的机制研究[J]. 中国中西医结合消化杂志, 2003,11(5): 287-289.
[12] 朱立俏.不同型号大孔吸附树脂对莱菔子水提液中萝卜苷的吸附性考察[J].山东中医药大学学报,2015,39(1):88-90.
[13] 朱立俏,盛华刚,周洪雷.莱菔子提取液中萝卜苷的大鼠肠吸收特性考察[J].中国实验方剂学杂志,2014,20(16):48-51.
[14] 王群,孟祥红,吕文海,等.炒莱菔子中萝卜苷和芥子碱乙醇提取工艺优选[J].中成药,2013,35(9):1911-1914.
[15] 夏青松,卢金清,黎强.HS-SPME-GC-MS联用分析炒制前后莱菔子中挥发性成分变化[J].中国实验方剂学杂志,2017,23(2):57-61.
[16] 金洪光,李玉全,凌云,等.莱菔子化学成分的分离与鉴定[J].食品科学,2018,39(6):35-40.
[17] Tung-Ting Sham,Ailsa Chui-Ying Yuen,Yam-Fung Ng,et al.A review of the Phytochemistry and Pharmacological Activities of Raphani Semen[J].Evidence-Based Complementary and Alternative Medicine,2013,2013:636194-636209.
[18] 任涛,吕文海. 以硫代葡萄糖苷相对含量优选莱菔子炮制工艺研究[J].中成药,2010,7:1159-1162.
[19] 毛茜,傅超美,胡慧玲,等. 川木香煨制前后主要药效成分在人工胃、肠液及大鼠离体胃肠共孵液中的稳定性研究[J]. 中国中药杂志,2012,6:785-789.
[20] 国家药典委员会.中华人民共和国药典:四部[S].北京:中国医药科技出版社,2015:119.
[21] 盛华刚,朱立俏,张津萌.金荞麦原花青素B2和表儿茶素在人工胃肠液中的含量变化[J].中国实验方剂学杂志,2014,20(4):77-80.
[22] 陈邦姣,朱立俏,孔得刚,等. 莱菔子中芥子碱硫氰酸盐在人工胃肠液中的稳定性研究[J]. 食品与药品,2014,3:188-190.