喀斯特山区草地施肥对放牧乌蒙半细毛羊抗氧化系统功能的影响
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摘要
为选择草地施肥的肥料种类,减少施肥对乌蒙半细毛羊产业的影响,在威宁县凉水沟种羊场多年生人工草地开展施肥和放牧试验。结果表明:凉水沟种羊场人工草地牧草铜含量相对较低,但仍高于乌蒙半细毛羊营养需要标准;施肥极显著增加牧草氮含量(P<0.01),各施肥处理之间没有明显差异。硫酸铵施肥显著增加牧草硫和锌含量(P<0.01),显著降低了牧草硒的含量(P<0.01)。放牧试验结束时,施硫酸铵草地的乌蒙半细毛羊血液中铜、铁和硒的含量极显著低于施硝酸铵草地和对照组(P<0.01),动物血液锌和硫含量极显著高于硝酸铵施肥牧场和对照组(P<0.01)。硫酸铵施肥牧场的乌蒙半细毛羊血红蛋白、红细胞压积容量极显著低于硝酸铵施肥牧场和对照牧场(P<0.01),血清铜蓝蛋白含量、血清超氧化物歧化酶活力、血清谷胱甘肽过氧化物酶活力和血清过氧化氢酶等抗氧化酶的活力极显著低于硝酸铵施肥牧场和对照牧场(P<0.01),丙二醛的含量显著高于硝酸铵施肥牧场和对照牧场(P<0.01)。血液其他矿质元素和血液指标及血清生化值在2个施肥处理和对照之间均差异不显著。因此得出结论,草地施肥的种类需要根据土壤矿物质分布和含量情况确定,铜含量低的牧场不适合硫酸铵施肥,硫酸铵施肥明显影响了乌蒙半细毛羊机体抗氧化系统功能。
To identify the effect of different nitrogen fertilizer on grassland,fertilization and grazing treatments were applied to perennial grassland grazed by Wumeng semi-fine wool sheep in Weining County.The results showed that copper content in grasses was relatively low,but was higher than the minimum requirement for sheep.Application of nitrogen significantly increase nitrogen content(P<0.01)in herbage but there were no differences among different forms of nitrogen.Ammonium sulfate significantly increased the sulfur and zinc content(P<0.01)of herbage,but significantly decreased the selenium content(P<0.01).At the end of grazing experiment,copper,iron and selenium concentration in the blood of sheep grazing grassland treated with ammonium sulfate was significantly lower(P<0.01)than in sheep grazing ammonium nitrate treated grassland;blood zinc and sulfur were also significantly higher(P<0.01).Sheep grazing ammonium sulfate treated pasture had significantly lower(P<0.01)blood hemoglobin and hematocrit capacity than sheep grazing pasture treated with ammonium nitrate and the control(no fertilizer);antioxidant enzymes including serum ceruloplasmin,serum superoxide dismutase and serum glutathione peroxidase were significantly lower(P<0.01)than in sheep grazing the pasture treated with ammonium nitrate and the control.Malondialdehyde(MDA)content was also higher(P<0.01).There were no differences in other blood mineral element concentrations,blood indexes and serum biochemical values.It was concluded that fertilizer selection should be based on soil mineral content;grassland with low copper content is not suitable for ammonium sulfate fertilizer.Ammonium sulfate fertilizer significantly affected the antioxidant system function.
To identify the effect of different nitrogen fertilizer on grassland,fertilization and grazing treatments were applied to perennial grassland grazed by Wumeng semi-fine wool sheep in Weining County.The results showed that copper content in grasses was relatively low,but was higher than the minimum requirement for sheep.Application of nitrogen significantly increase nitrogen content(P<0.01)in herbage but there were no differences among different forms of nitrogen.Ammonium sulfate significantly increased the sulfur and zinc content(P<0.01)of herbage,but significantly decreased the selenium content(P<0.01).At the end of grazing experiment,copper,iron and selenium concentration in the blood of sheep grazing grassland treated with ammonium sulfate was significantly lower(P<0.01)than in sheep grazing ammonium nitrate treated grassland;blood zinc and sulfur were also significantly higher(P<0.01).Sheep grazing ammonium sulfate treated pasture had significantly lower(P<0.01)blood hemoglobin and hematocrit capacity than sheep grazing pasture treated with ammonium nitrate and the control(no fertilizer);antioxidant enzymes including serum ceruloplasmin,serum superoxide dismutase and serum glutathione peroxidase were significantly lower(P<0.01)than in sheep grazing the pasture treated with ammonium nitrate and the control.Malondialdehyde(MDA)content was also higher(P<0.01).There were no differences in other blood mineral element concentrations,blood indexes and serum biochemical values.It was concluded that fertilizer selection should be based on soil mineral content;grassland with low copper content is not suitable for ammonium sulfate fertilizer.Ammonium sulfate fertilizer significantly affected the antioxidant system function.
引文
[1]Zhou S S,Sun H,Liao J F,et al.Effects of grazing on the vegetation composition of Lolium perenne and Trifolium repens mixed pasture.Pratacultural Science,2012,29(5):814-820.周姗姗,孙红,廖加法,等.放牧对黑麦草+白三叶混播草地植被构成的作用.草业科学,2012,29(5):814-820.
[2]Luo J C,Zhou M,Wang Z L,et al.Fluxion and regulation of selenium in soil,plants,and animals in grassland grazing system.Pratacultural Science,2017,34(4):869-880.罗建川,周梅,王宗礼,等.硒在草地放牧系统“土壤-植物-动物”间的流动与调控.草业科学,2017,34(4):869-880.
[3]Li K,Zhu J Z.Effect of boron and molybdenum on the seed yield alfalfa.Pratacultural Science,2009,26(1):61-63.李科,朱进忠.硼、钼元素对苜蓿种子的增产效果.草业科学,2009,26(1):61-63.
[4]Shen X Y,Chi Y K,Xiong K N,et al.Serum biochemical values and mineral contents of tissues in Guizhou semi-fine wool sheep.Journal of Animals and Veterinary Advance,2013,12(11):1078-1080.
[5]Li L J,Shen X Y.Development progress and breeding status of Guizhou semi-fine wool sheep.Guizhou Agricultural Sciences,2010,38(11):182-184.李丽娟,申小云.贵州半细毛羊的培育历程与养殖现状.贵州农业科学,2010,38(11):182-184.
[6]Shen X Y,Jiang H M,Yuan R,et al.Effect of nitrogen fertilizer source on mineral element content of forage and in the blood of grazing Guizhou semi-fine sheep.Acta Prataculturae Sinica,2012,21(3):275-280.申小云,蒋会梅,苑荣,等.草地施肥对牧草和放牧贵州半细毛羊的影响.草业学报,2012,21(3):275-280.
[7]Shen X Y.Effect of nitrogenous fertilizer treatment on mineral metabolism in grazing yaks.Agricultural Sciences in China,2009,8(3):361-368.
[8]Shen X Y,Du G Z,Li H.Studies of a naturally occurring molybdenum-induced copper deficiency in the yak.The Veterinary Journal,2006,171(2):352-357.
[9]Tiffany M E,Mc Dowell L R,O’connor G A,et al.Effects of residual and reapplied biosolids on performance and mineral status of grazing beef steers.Journal of Animal Science,2002,80:260-266.
[10]Wang K,Tang R H,Xu H B,et al.Trace Element in Life Science.Beijing:China Metrology Press,1999:321-675.王夔,唐任环,徐辉碧,等.生命科学中的微量元素.北京:中国计量出版社,1999:321-675.
[11]Wang Z Y,Cao G X,Hu Z Z,et al.Mineral Element Metabolism and Animal Disease.Shanghai:Shanghai Science and Technology Press,1995:231-356.王宗元,曹光辛,胡在朝,等.动物矿物质营养代谢与疾病.上海:上海科学技术出版社,1995:231-356.
[12]Arthington J D,Rechcigl J E,Yost G P,et al.Effect of ammonium sulfate fertilization on bahiagrass quality and copper metabolism in grazing beef cattle.Journal of Animal Science,2002,80:2507-2512.
[13]Hardt P F,Greene L W.Forage mineral concentration,animal performance,and mineral status of heifers grazing cereal pastures fertilized with sulfur.Journal of Animal Science,1991,69:2310-2320.
[14]Shen X Y,Du G Z,Chen Y M,et al.Copper deficiency in Guizhou semi-fine sheep on pasture in western China.The Canadian Veterinary Journal,2006,47:902-906.
[15]Chan Y H,Siu C W,Yiu K H,et al.Adverse systemic arterial function in patients with selenium deficiency.Journal of Nutrition Health and Aging,2012,16(1):85-88.
[16]Hurst R,Siyame E W,Young S D,et al.Soil-type influences human selenium status and underlies widespread selenium deficiency risks in Malawi.Scientific Reports,2013,3(2):1425.
[17]Liu C P,Fu J,Lin S L,et al.Effects of dietary selenium deficiency on mRNA levels of twenty-one selenoprotein genes in the liver of layer chicken.Biological Trace Element Research,2014,159(3):192-198.
[18]Nangliya V I,Sharma A,Yadav D,et al.Study of trace elements in liver cirrhosis patients and their role in prognosis of disease.Biological Trace Element Research,2015,160(1):135-141.
[19]Xu S W,Yao H D,Zhang J,et al.The oxidative damage and misbalance of calcium homeostasis in brain of chicken induced by selenium deficiency.Biological Trace Element Research,2013,151(2):225-233.
[20]Shen X Y.Forage strategy of Przewalski’s gazelle under selenium stress.Acta Ecologica Sinica,2009,29(6):2775-2781.申小云.硒胁迫下普氏原羚的采食对策.生态学报,2009,29(6):2775-2781.
[21]Coleman R.The importance of sulfur as a plant nutrient in world crop production.Soil Science,1966,101:230-239.
[22]Martin W E,Walker T W.Sulfur requirement and fertilization of pasture and forage crops.Soil Science,1966,101:248-257.
[23]Rechcigl J E.Sulphur fertilization improves bahiagrass pasture.Better Crops Plant Food,1991,75:22-24.
[24]Shen X Y,Li X,Zhang R D.Studies of“Unsteady Gait Disease”of the Tibetan Gazelle(Procapra picticaudata).Journal of Wildlife Diseases,2010,46(2):560-563.
[25]Kelly C W,Brian C T,Itai S,et al.Fermentation,hydrogen,and sulfur metabolism in multiple uncultivated bacterial phyla.Science,2012,337(6102):1661-1665.
[26]Jeffrey M,Duff J P,Higgins R J,et al.Polioencephalomalacia associated with the ingestion of ammonium sulphate by sheep and cattle.Veterinary Record,1994,134:343-348.
[27]Smart M E,Cohen R,Christensen D A,et al.The effect of sulphate removal from the drinking water on the plasma and liver copper and zinc concentrations of beef cows and their calves.Canadian Journal of Animal Science,1986,66:669-680.
[28]Suttle N F.Mineral Nutrition of Livestock.4th Edition.London:CABI Publishing,2010:597.
[2]Luo J C,Zhou M,Wang Z L,et al.Fluxion and regulation of selenium in soil,plants,and animals in grassland grazing system.Pratacultural Science,2017,34(4):869-880.罗建川,周梅,王宗礼,等.硒在草地放牧系统“土壤-植物-动物”间的流动与调控.草业科学,2017,34(4):869-880.
[3]Li K,Zhu J Z.Effect of boron and molybdenum on the seed yield alfalfa.Pratacultural Science,2009,26(1):61-63.李科,朱进忠.硼、钼元素对苜蓿种子的增产效果.草业科学,2009,26(1):61-63.
[4]Shen X Y,Chi Y K,Xiong K N,et al.Serum biochemical values and mineral contents of tissues in Guizhou semi-fine wool sheep.Journal of Animals and Veterinary Advance,2013,12(11):1078-1080.
[5]Li L J,Shen X Y.Development progress and breeding status of Guizhou semi-fine wool sheep.Guizhou Agricultural Sciences,2010,38(11):182-184.李丽娟,申小云.贵州半细毛羊的培育历程与养殖现状.贵州农业科学,2010,38(11):182-184.
[6]Shen X Y,Jiang H M,Yuan R,et al.Effect of nitrogen fertilizer source on mineral element content of forage and in the blood of grazing Guizhou semi-fine sheep.Acta Prataculturae Sinica,2012,21(3):275-280.申小云,蒋会梅,苑荣,等.草地施肥对牧草和放牧贵州半细毛羊的影响.草业学报,2012,21(3):275-280.
[7]Shen X Y.Effect of nitrogenous fertilizer treatment on mineral metabolism in grazing yaks.Agricultural Sciences in China,2009,8(3):361-368.
[8]Shen X Y,Du G Z,Li H.Studies of a naturally occurring molybdenum-induced copper deficiency in the yak.The Veterinary Journal,2006,171(2):352-357.
[9]Tiffany M E,Mc Dowell L R,O’connor G A,et al.Effects of residual and reapplied biosolids on performance and mineral status of grazing beef steers.Journal of Animal Science,2002,80:260-266.
[10]Wang K,Tang R H,Xu H B,et al.Trace Element in Life Science.Beijing:China Metrology Press,1999:321-675.王夔,唐任环,徐辉碧,等.生命科学中的微量元素.北京:中国计量出版社,1999:321-675.
[11]Wang Z Y,Cao G X,Hu Z Z,et al.Mineral Element Metabolism and Animal Disease.Shanghai:Shanghai Science and Technology Press,1995:231-356.王宗元,曹光辛,胡在朝,等.动物矿物质营养代谢与疾病.上海:上海科学技术出版社,1995:231-356.
[12]Arthington J D,Rechcigl J E,Yost G P,et al.Effect of ammonium sulfate fertilization on bahiagrass quality and copper metabolism in grazing beef cattle.Journal of Animal Science,2002,80:2507-2512.
[13]Hardt P F,Greene L W.Forage mineral concentration,animal performance,and mineral status of heifers grazing cereal pastures fertilized with sulfur.Journal of Animal Science,1991,69:2310-2320.
[14]Shen X Y,Du G Z,Chen Y M,et al.Copper deficiency in Guizhou semi-fine sheep on pasture in western China.The Canadian Veterinary Journal,2006,47:902-906.
[15]Chan Y H,Siu C W,Yiu K H,et al.Adverse systemic arterial function in patients with selenium deficiency.Journal of Nutrition Health and Aging,2012,16(1):85-88.
[16]Hurst R,Siyame E W,Young S D,et al.Soil-type influences human selenium status and underlies widespread selenium deficiency risks in Malawi.Scientific Reports,2013,3(2):1425.
[17]Liu C P,Fu J,Lin S L,et al.Effects of dietary selenium deficiency on mRNA levels of twenty-one selenoprotein genes in the liver of layer chicken.Biological Trace Element Research,2014,159(3):192-198.
[18]Nangliya V I,Sharma A,Yadav D,et al.Study of trace elements in liver cirrhosis patients and their role in prognosis of disease.Biological Trace Element Research,2015,160(1):135-141.
[19]Xu S W,Yao H D,Zhang J,et al.The oxidative damage and misbalance of calcium homeostasis in brain of chicken induced by selenium deficiency.Biological Trace Element Research,2013,151(2):225-233.
[20]Shen X Y.Forage strategy of Przewalski’s gazelle under selenium stress.Acta Ecologica Sinica,2009,29(6):2775-2781.申小云.硒胁迫下普氏原羚的采食对策.生态学报,2009,29(6):2775-2781.
[21]Coleman R.The importance of sulfur as a plant nutrient in world crop production.Soil Science,1966,101:230-239.
[22]Martin W E,Walker T W.Sulfur requirement and fertilization of pasture and forage crops.Soil Science,1966,101:248-257.
[23]Rechcigl J E.Sulphur fertilization improves bahiagrass pasture.Better Crops Plant Food,1991,75:22-24.
[24]Shen X Y,Li X,Zhang R D.Studies of“Unsteady Gait Disease”of the Tibetan Gazelle(Procapra picticaudata).Journal of Wildlife Diseases,2010,46(2):560-563.
[25]Kelly C W,Brian C T,Itai S,et al.Fermentation,hydrogen,and sulfur metabolism in multiple uncultivated bacterial phyla.Science,2012,337(6102):1661-1665.
[26]Jeffrey M,Duff J P,Higgins R J,et al.Polioencephalomalacia associated with the ingestion of ammonium sulphate by sheep and cattle.Veterinary Record,1994,134:343-348.
[27]Smart M E,Cohen R,Christensen D A,et al.The effect of sulphate removal from the drinking water on the plasma and liver copper and zinc concentrations of beef cows and their calves.Canadian Journal of Animal Science,1986,66:669-680.
[28]Suttle N F.Mineral Nutrition of Livestock.4th Edition.London:CABI Publishing,2010:597.