穗加精液分析自动检测系统与血细胞计数板人工计数法在小鼠精液分析中的应用
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摘要
目的比较穗加精液分析(SSA)自动检测系统与血细胞计数板人工计数法在小鼠精液分析中的功能及效果。方法采用SSA自动检测系统和血细胞计数板分别对成年雄性C57BL/6小鼠的精子一般形态、浓度、运动学参数及活力进行检测,并进行统计学分析。结果采用SSA自动检测系统计数小鼠的精子浓度显著高于血细胞计数板人工计数法(P<0.05)。SSA自动检测系统检测小鼠精子直线速率为(25.41±6.42)μm/s,曲线速率为(74.13±5.82)μm/s,直线性为(24.63±8.43)%,前向性为(41.00±9.62)%。小鼠的精子活动率SSA自动检测系统高于血细胞计数板人工计数法(P<0.05)。结论 SSA自动检测系统能够直接观察精子的形态、运动并进行相应的参数分析,准确率高,操作简便,为小鼠精液的分析提供了方便可靠的方法。
Objective To compare the functions and differences of Suiplus semen analysis(SSA) automatic detection system and manual counting using hemocytometer in mouse semen analysis. Methods The general morphology, concentration, kinematic parameters and motility of sperm samples of adult male C57 BL/6 mice were measured by the above two methods respectively. The data were statistically analyzed. Results The sperm concentration detected by the SSA system was significantly higher than that detected by manual counting(P<0.05). The results of the SSA system showed that the straight line velocity was(25.41±6.42) μm/s,the elocity in curvilinear velocity was(74.13±5.82) μm/s,the linearity was(24.63±8.43) %,and the straightness was(41.00±9.62) %. The proportion of motile sperm detected by the SSA system was significantly higher than that detected by manual counting(P<0.05). Conclusion SSA automatic detection system can directly observe the morphology and movement of sperm,and analyze the corresponding parameters simply but accurately,which provides a convenient and reliable method for semen analysis.
Objective To compare the functions and differences of Suiplus semen analysis(SSA) automatic detection system and manual counting using hemocytometer in mouse semen analysis. Methods The general morphology, concentration, kinematic parameters and motility of sperm samples of adult male C57 BL/6 mice were measured by the above two methods respectively. The data were statistically analyzed. Results The sperm concentration detected by the SSA system was significantly higher than that detected by manual counting(P<0.05). The results of the SSA system showed that the straight line velocity was(25.41±6.42) μm/s,the elocity in curvilinear velocity was(74.13±5.82) μm/s,the linearity was(24.63±8.43) %,and the straightness was(41.00±9.62) %. The proportion of motile sperm detected by the SSA system was significantly higher than that detected by manual counting(P<0.05). Conclusion SSA automatic detection system can directly observe the morphology and movement of sperm,and analyze the corresponding parameters simply but accurately,which provides a convenient and reliable method for semen analysis.
引文
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[18]陆金春,岳茹倩,冯瑞祥,等.精子计数池深度对精子活力影响的实验研究[J].中华男科学杂志,2013,19(9):776-779.LU Jinchun,YUE Ruqian,FENG Ruixiang,et al.Influence of the depth of the sperm counting chamber on sperm motility[J].National Journal of Andrology,2013,19(9):776-779.
[19]Brito LF,Althouse GC,Aurich C,et al.Andrology laboratory review:evaluation of sperm concentration[J].Theriogenology,2016,85(9):1507-1527.
[20]LU Jinchun,YUE Ruqian,FENG Ruixiang,et al.Accuracy evaluation of the depth of six kinds of sperm counting chambers for both manual and computer-aided semen analyses[J].Int J Fertil Steril,2016,9(4):527-533.
[21]Auger J,Jouannet P,Eustache F.Another look at human sperm morphology[J].Hum Reprod,2016,31(1):10-23.
[22]王丽琼,陈建伟,齐德生,等.精子DNA单双链损伤与精子浓度的关系研究[J].中国性科学,2017,26(3):87-90.WANG Liqiong,CHEN Jianwei,QI Desheng,et al.Study on the relationship between sperm nuclear DNA damage and sperm production[J].The Chinese Journal of Human Sexuality,2017,26(3):87-90.
[23]刘政,刘子巍,林冲,等.亚慢性低浓度苯暴露对小鼠精子活力的影响[J].毒理学杂志,2013,27(3):207-209.
[24]Young SA,Miyata H,Satouh Y,et al.CABYR is essential for fibrous sheath integrity and progressive motility in mouse spermatozoa[J].J Cell Sci,2016,129(23):4379-4387.
[25]Miura N,Ohtani K,Hasegawa T,et al.High sensitivity of testicular function to titanium nanoparticles[J].J Toxicol Sci,2017,42(3):359-366.
[26]张永涛.男性不育患者精液常规检查结果中的异常项目分析[J].河南医学研究,2017,26(15):2715-2716.
[2]Adewoyin M,Ibrahim M,Roszaman R,et al.Male infertility:the effect of natural antioxidants and phytocompounds on seminal oxidative stress[J].Diseases,2017,5(1):9.doi:10.3390/diseases5010009
[3]吴园园,韩玉芳,王瑞红,等.计算机辅助精液分析与手工计数法检测精液的比较研究[J].国际检验医学杂志,2017,38(12):1726-1727.doi:10.3969/j.issn.1673-4130.2017.12.061
[4]PENG Nanni,ZOU Xiangli,LI Lingwei.Comparison of different counting chambers using a computer-assisted semen analyzer[J].Syst Biol Reprod Med,2015,61(5):307-313.
[5]申涌,刘咏梅.枸杞多糖对甲醛所致雄性小鼠生殖毒性的拮抗作用[J].贵州医科大学学报,2017,42(4):440-443.SHEN Yong,LIU Yongmei.Study on the antagonism of lycium barbarum polysaccharides on formaldehyde induced male mice reproductive toxicity[J].Journal of Guizhou Medical University,2017,42(4):440-443.
[6]Kumari K,Capstick M,Cassara AM,et al.Effects of intermediate frequency magnetic fields on male fertility indicators in mice[J].Environ Res,2017,157:64-70.doi:10.1016/j.envres.2017.05.014
[7]李文娟,王晋.间歇低氧对小鼠精子生成数量及活力的影响[J].中国继续医学教育,2015,7(18):82-83.LI Wenjuan,WANG Jin.The effects of intermittent hypoxia on sperm number and activity in mice[J].China Continuing Medical Education,2015,7(18):82-83.
[8]Miller MR,Mansell SA,Meyers SA,et al.Flagellar ion channels of sperm:similarities and differences between species[J].Cell Calcium,2015,58(1):105-113.
[9]世界卫生组织.人类精液检查及处理实验室手册[M].5版.北京:人民卫生出版社,2011:117-118.
[10]Nikmehr B,Bazrafkan M,Hassanzadeh G,et al.The correlation of gene expression of inflammasome indicators and impaired fertility in rat model of spinal cord injury:a time course study[J].Urol J,2017,14(6):5057-5063.
[11]史晓蓉,刘舒瑜,陈亚,等.芹菜素对小鼠精液参数影响的研究[J].中华男科学杂志,2010,16(9):778-782.SHI Xiaorong,LIU Shuyu,CHEN Ya,et al.Apigenin affects semen parameters in male mice[J].National Journal of Andrology,2010,16(9):778-782.
[12]Nelson VL,Negrón AL,Reid I,et al.Loss of PI3K p110αin the adipose tissue results in infertility and delayed puberty onset in male mice[J].Biomed Res Int,2017,2017:3756089.doi:10.1155/2017/3756089
[13]ZANG Zhijun,FANG Youqiang,JI Suyun,et al.Formaldehyde inhibits sexual behavior and expression of steroidogenic enzymes in the testes of mice[J].J Sex Med,2017,14(11):1297-1306.
[14]Moretti E,Collodel G,Mazzi L,et al.Ultrastructural study of spermatogenesis in KSR2 deficient mice[J].Transgenic Res,2015,24(4):741-751.
[15]Dardmeh F,Alipour H,Gazerani P,et al.Lactobacillus rhamnosus PB01(DSM 14870)supplementation affects markers of sperm kinematic parameters in a diet-induced obesity mice model[J].PLo S One,2017,12(10):e0185964.doi:10.1371/journal.pone.0185964.
[16]Weng Z,Ohtani K,Suda M,et al.Assessment of the reproductive toxicity of inhalation exposure to ethyl tertiary butyl ether in male mice with normal,low active and inactive ALDH2[J].Arch Toxicol,2014,88(4):1007-1021.
[17]WANG Huafeng,CHANG Meng,PENG Tingting,et al.Exposure to cadmium impairs sperm functions by reducing Cat Sper in mice[J].Cell Physiol Biochem,2017,42(1):44-54.
[18]陆金春,岳茹倩,冯瑞祥,等.精子计数池深度对精子活力影响的实验研究[J].中华男科学杂志,2013,19(9):776-779.LU Jinchun,YUE Ruqian,FENG Ruixiang,et al.Influence of the depth of the sperm counting chamber on sperm motility[J].National Journal of Andrology,2013,19(9):776-779.
[19]Brito LF,Althouse GC,Aurich C,et al.Andrology laboratory review:evaluation of sperm concentration[J].Theriogenology,2016,85(9):1507-1527.
[20]LU Jinchun,YUE Ruqian,FENG Ruixiang,et al.Accuracy evaluation of the depth of six kinds of sperm counting chambers for both manual and computer-aided semen analyses[J].Int J Fertil Steril,2016,9(4):527-533.
[21]Auger J,Jouannet P,Eustache F.Another look at human sperm morphology[J].Hum Reprod,2016,31(1):10-23.
[22]王丽琼,陈建伟,齐德生,等.精子DNA单双链损伤与精子浓度的关系研究[J].中国性科学,2017,26(3):87-90.WANG Liqiong,CHEN Jianwei,QI Desheng,et al.Study on the relationship between sperm nuclear DNA damage and sperm production[J].The Chinese Journal of Human Sexuality,2017,26(3):87-90.
[23]刘政,刘子巍,林冲,等.亚慢性低浓度苯暴露对小鼠精子活力的影响[J].毒理学杂志,2013,27(3):207-209.
[24]Young SA,Miyata H,Satouh Y,et al.CABYR is essential for fibrous sheath integrity and progressive motility in mouse spermatozoa[J].J Cell Sci,2016,129(23):4379-4387.
[25]Miura N,Ohtani K,Hasegawa T,et al.High sensitivity of testicular function to titanium nanoparticles[J].J Toxicol Sci,2017,42(3):359-366.
[26]张永涛.男性不育患者精液常规检查结果中的异常项目分析[J].河南医学研究,2017,26(15):2715-2716.