白细胞分类人工镜检与全自动血细胞分析仪检查结果的比较
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摘要
选新生入学体检血标本75例,另选门诊血常规检查的患者126例中单核细胞(MO)占15%~25%的血标本43例,分别进行白细胞人工镜检分类和全自动血细胞分析仪分类。新生入学体检标本人工分类和仪器分类的中性粒细胞(NE)、淋巴细胞(LY)和MO的百分率均无显著性差异(P>0.05);嗜酸性粒细胞(EO)和嗜碱性粒细胞(BA)两组比较有显著性差异(P<0.05)。MO百分率在15%~25%的标本人工分类和仪器分类的NE、EO和MO的百分率均无显著性差异(P>0.05),人工法测得的BA和LY百分率明显低于仪器法,两组比较有显著性差异(P<0.05)。以人工镜检为金标准,全自动血细胞分析仪的白细胞分类结果准确,但不能完全取代人工镜检,尤其对于异常标本,应把两种方法结合起来才能确保检测结果的可靠。
A total of 75 blood samples from newly enrolled students and 43 cases in which the mononuclear cells accounted for 15% to 25% from 126 blood samples of outpatients with routine blood tests were selected. Neutrophil classification entrance examination specimens of artificial classification and instrument(NE)and lymphocyte(LY)showed no significant difference and the percentage of MO(P>0.05); Eosinophils(EO)and Eosinophil(BA)between the two groups had significant difference(P<0.05). There was no significant difference in the percentage of NE、EO and MO between the MO percentage of 15% to 25% specimens and the NE、EO and MO of the instrument classification. The percentage of BA and LY measured by manual method was significantly lower than that by the instrument method. There was a significant difference between the two groups(P<0.05). Taking artificial microscopy as the gold standard,the white blood cell classification results of the automatic blood cell analyzer are accurate,but they cannot completely replace artificial microscopy. Especially for abnormal specimens, the two methods should be combined to ensure the reliability of the test results.
A total of 75 blood samples from newly enrolled students and 43 cases in which the mononuclear cells accounted for 15% to 25% from 126 blood samples of outpatients with routine blood tests were selected. Neutrophil classification entrance examination specimens of artificial classification and instrument(NE)and lymphocyte(LY)showed no significant difference and the percentage of MO(P>0.05); Eosinophils(EO)and Eosinophil(BA)between the two groups had significant difference(P<0.05). There was no significant difference in the percentage of NE、EO and MO between the MO percentage of 15% to 25% specimens and the NE、EO and MO of the instrument classification. The percentage of BA and LY measured by manual method was significantly lower than that by the instrument method. There was a significant difference between the two groups(P<0.05). Taking artificial microscopy as the gold standard,the white blood cell classification results of the automatic blood cell analyzer are accurate,but they cannot completely replace artificial microscopy. Especially for abnormal specimens, the two methods should be combined to ensure the reliability of the test results.
引文
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[2]张丽娟,陈春丽,舒晓春.迈瑞BC-5500全自动血细胞分析仪异常提示的可靠性分析[J].中国医药,2010,5(11):1069-1070.
[3]周丹,许煊.检测炎症指标作为川崎病的早期诊断及冠状脉扩张高危因素探讨[J].中国临床医生杂志,2015,43(11):37-39.
[4]张茹,王志娟,杨靖娴.红细胞冷凝集对全自动血细胞分析仪检测血细胞参数的影响[J].临床误诊误治,2014,27(4):81-83.
[5]李明勇,李焱鑫,陈梅等.血细胞分析仪白细胞分类计数量值溯源性的建立[J].现代检验医学杂志,2011,26(6):150-152.
[6]韩冰,高阳,李康,等.Sysmex XT-1800i血细胞分析仪白细胞分类报警系统(Q-Flag)的临床应用价值[J].国际检验医学杂志,2015,36(14):2013-2014.
[7]杨红斌,朱庆文,喻战军,等.分离胶真空采血管中血细胞比容对血糖、钾测定结果的影响[J].中国医刊,2014,49(6):42-45.
[8]Novis DA,Walsh M,Wilkinson D,et al.Laboratory productivity and the rate of manual peripheral blood smear review:a College of American Pathologists Q-Probes study of 95,141 complete blood count determinations performed in 263 institutions[J].Arch Pathol Lab Med,2006,130(5):596-601.
[9]何振辉,翁闪凡,陈颖榆,等.Sysmex XE-5000血细胞分析仪计数胸腔积液细胞的评价[J].广东医学,2014,35(2):229-231.
[10]陈建霞,黄衍锋,张旭.XE-5000全自动血细胞分析仪检测白细胞分类与人工镜检结果对比分析[J].中国当代医药,2015,22(12):124-126.
[11]张福海.我院血细胞分析仪复检规则的制定及评价[J].临床误诊误治,2013,26(12):40-42.
[12]李瑞祥.血液分析仪白细胞分类计数结果与瑞氏染色镜检结果的比较[J].广西医学,2015,37(3):403-404.