脂血标本对时间分辨荧光免疫分析法测定游离雌三醇水平的影响
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摘要
目的探讨脂血标本对时间分辨荧光免疫分析法检测游离雌三醇水平的影响。方法收集男性呈正常和乳糜样外观的不同水平脂血标本,与不同水平的游离雌三醇定值血清混匀,制成混合血清,用时间分辨荧光分析法测定其游离雌三醇水平以评价不同乳糜样和正常外观脂血标本对游离雌三醇水平的影响。结果对于乳糜样外观标本,轻度脂血可使游离雌三醇水平增高,中度及高度脂血则使游离雌三醇水平降低,且对游离雌三醇水平的影响十分接近。外观正常的中度脂血对游离雌三醇水平无显著干扰。结论乳糜样外观脂血对时间分辨荧光分析法测定游离雌三醇存在不同的影响,进而影响唐氏筛查的准确度。
Objective To investigate the effect of different degree lipidemia on time-resolved fluoroimmunoassay(TRFIA)for determination of unconjugated estriol(uE3).Methods Mixed serum was prepared by collecting different levels of lipidemia samples which were normal and chylous appearance from male and by mixing with definite value serum of uE3.The levels of uE3 in the samples were measured by TRFIA and the effect of lipidemia on TRFIA for determination of uE3 was evaluated.Results For the appearance of chylous specimens,mild lipidemia increased uE3,mid-or hiper-lipidemia samples reduced uE3 and the effect of both was considerable.Conclusion The chylous lipidemia has variant degree of influence to TRFIA for determination of uE3,then the results effect accuracy of Down′s screening.
Objective To investigate the effect of different degree lipidemia on time-resolved fluoroimmunoassay(TRFIA)for determination of unconjugated estriol(uE3).Methods Mixed serum was prepared by collecting different levels of lipidemia samples which were normal and chylous appearance from male and by mixing with definite value serum of uE3.The levels of uE3 in the samples were measured by TRFIA and the effect of lipidemia on TRFIA for determination of uE3 was evaluated.Results For the appearance of chylous specimens,mild lipidemia increased uE3,mid-or hiper-lipidemia samples reduced uE3 and the effect of both was considerable.Conclusion The chylous lipidemia has variant degree of influence to TRFIA for determination of uE3,then the results effect accuracy of Down′s screening.
引文
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[4]薛艳春,朱伟,沈丽,等.测定孕妇血清游离雌三醇含量预测早产的发生[J].中外医疗,2012,31(21):38-39.
[5]白雪,范祎.游离雌三醇和人胎盘催乳素测定在子痫前期的意义[J].国际检验医学杂志,2015,36(13):1870-1871.
[6]张辉亮,黄树华,刘健玲.重度乳糜血标本对三种常见类型生化检测方法的影响[J].现代检验医学杂志,2015,30(1):128-131.
[7]徐玉兵,高春芳,赵琳.脂血对Sysmex XE-2100D血液分析仪检测指标的影响研究[J].检验医学,2012,27(12):1017-1020.
[8]郭华国,桂瑞丰,汪宏,等.高血脂、肝素对乙型肝炎病毒DNA实时荧光定量PCR测定干扰机制的探讨[J].现代检验医学杂志,2007,22(1):58-60.
[9]温秀明,彭及良,李丹红,等.中、重度乳糜血对血液5项检验指标检测结果的影响分析[J].国际输血及血液学杂志,2015,38(4):312-316.
[2]Reynolds T,Vranken G,Van Nueten J,et al.Down′s syndrome screening:populationstatistic dependency of screening performanc[J].Clin Chem Lab Med,2008,46(5):639-647.
[3]巩敏红,王玉.孕中期唐氏综合征产前筛查两种方式筛查效率比较[J].吉林医学,2014,35(11):2392-2393.
[4]薛艳春,朱伟,沈丽,等.测定孕妇血清游离雌三醇含量预测早产的发生[J].中外医疗,2012,31(21):38-39.
[5]白雪,范祎.游离雌三醇和人胎盘催乳素测定在子痫前期的意义[J].国际检验医学杂志,2015,36(13):1870-1871.
[6]张辉亮,黄树华,刘健玲.重度乳糜血标本对三种常见类型生化检测方法的影响[J].现代检验医学杂志,2015,30(1):128-131.
[7]徐玉兵,高春芳,赵琳.脂血对Sysmex XE-2100D血液分析仪检测指标的影响研究[J].检验医学,2012,27(12):1017-1020.
[8]郭华国,桂瑞丰,汪宏,等.高血脂、肝素对乙型肝炎病毒DNA实时荧光定量PCR测定干扰机制的探讨[J].现代检验医学杂志,2007,22(1):58-60.
[9]温秀明,彭及良,李丹红,等.中、重度乳糜血对血液5项检验指标检测结果的影响分析[J].国际输血及血液学杂志,2015,38(4):312-316.