没食子酸在大鼠体内的组织分布研究
|
摘要
目的:建立高效液相色谱法(HPLC)测定大鼠各组织中没食子酸含量的方法,研究没食子酸在大鼠体内的组织分布特征。方法:大鼠按50 mg·kg~(-1)灌胃给予没食子酸后取各组织,采用沉淀蛋白法预处理生物组织样品,色谱柱:Fortis-C_(18)柱(250 mm×4.6 mm,5μm),流动相:甲醇-0.1%磷酸水=25∶75;检测波长273 nm;柱温30℃;进样体积20μl;流速1 ml·min-1,测定各组织中没食子酸的含量。结果:各组织中没食子酸的浓度在0.50~128.00μg·ml~(-1)范围内线性关系良好。灌胃0.5 h后各组织中均能检测到没食子酸,在各组织分布中,小肠中含量最高,肺中含量最低。结论:建立了大鼠组织中没食子酸的HPLC测定方法,该方法简便、灵敏度高、专属性强,结果准确;由此法测得没食子酸组织分布迅速且广泛,主要分布在小肠、肾、肝脏组织中。
Objective: To establish a high performance liquid chromatography method for the determination of gallic acid in tissues of rats,and study the tissue distribution characteristics of gallic acid in rats. Methods: The tissues were obtained after the oral administration of 50 mg · kg~(-1) gallic acid,and prepared by protein precipitation. The analytical column was a Fortis-C_(18) column( 250 mm× 4. 6 mm,5 μm). The mobile phase was a mixture of methanol and 0. 1% phosphoric acid( 25 ∶ 75),the detection wavelength was set at 273 nm,the column temperature was 30℃,the injection volume was 20 μl,and the flow rate was 1 ml·min~(-1). Results: The calibration curves of gallic acid in the tissues were linear within the range of 0. 50-128. 00 μg·ml~(-1). Gallic acid could be detected out in all the tissues at 0. 5 h after the oral administration. The highest content of gallic acid was checked out in small intestine,and the lowest was detected out in the lung. Conclusion: An HPLC method was established to detect gallic acid in the tissues of rats. The method is simple,sensitive,specific and accurate. The distribution of gallic acid is quick and extensive mainly in small intestine,kidney and liver.
Objective: To establish a high performance liquid chromatography method for the determination of gallic acid in tissues of rats,and study the tissue distribution characteristics of gallic acid in rats. Methods: The tissues were obtained after the oral administration of 50 mg · kg~(-1) gallic acid,and prepared by protein precipitation. The analytical column was a Fortis-C_(18) column( 250 mm× 4. 6 mm,5 μm). The mobile phase was a mixture of methanol and 0. 1% phosphoric acid( 25 ∶ 75),the detection wavelength was set at 273 nm,the column temperature was 30℃,the injection volume was 20 μl,and the flow rate was 1 ml·min~(-1). Results: The calibration curves of gallic acid in the tissues were linear within the range of 0. 50-128. 00 μg·ml~(-1). Gallic acid could be detected out in all the tissues at 0. 5 h after the oral administration. The highest content of gallic acid was checked out in small intestine,and the lowest was detected out in the lung. Conclusion: An HPLC method was established to detect gallic acid in the tissues of rats. The method is simple,sensitive,specific and accurate. The distribution of gallic acid is quick and extensive mainly in small intestine,kidney and liver.
引文
1谢晓艳,刘洪涛,张吉,等.没食子酸体外抗氧化作用研究[J].重庆医科大学学报,2011,36(3):319-322
2 李立.HPLC法测定蒙药那如三味丸中没食子酸和胡椒碱的含量[J].中国药师,2015,18(10):1830-1832
3 Rattanata N,Klaynongsruang S,Daduang S,et al.Inhibitory effects of gallic acid isolatated from caesalpinia mimosoides Lamk on cholangiocarcinoma cell lines and foodborne pathogenic bacteria[J].Asian Pac J Cancer Prev,2016,17(3):1341-1345
4 Hsu W,,Chang SP,Lin LC,et al.Limonium sinense and gallic acid suppress hepatitis C virus infection by blocking early viral entry[J].Antiviral Res,2015,118:139-147
5 张庭秀,马李杰,范贤明,等.没食子酸诱导非小细胞肺癌A549细胞凋亡的机制研究[J].中国现代医学杂志,2018,28(19):16-22
6鄢文强,李彩霞,王冬滨,等.没食子酸对人食管癌细胞生长与凋亡的影响[J].实用医学杂志,2015,31(4):540-543
7 周静,周本宏,涂杰,等.石榴皮提取物中鞣花酸和没食子酸在大鼠体内的药代动力学研究[J].中国医药导报,2015,12(3):19-23
8王琳,陈涵.药物体外吸收、分布、代谢和排泄筛选模型[J].中国组织工程研究与临床康复,2008,12(9):9957-9961
9 高林晓,郭蒙,刘凤华,等.超声浸提-高效液相色谱法测定刺三加根和茎中没食子酸含量[J].中国食品添加剂,2018,(6):145-150
10张书,林世和,郭珩,等.HPLC法测定微米大黄炭中没食子酸的含量[J].中国药师,2013,16(12):1822-1824
11 刘莉,李婷婷,孔卫东,等.HPLC法测定肠炎宁胶囊中没食子酸的含量[J].内江科技,2017(3):23,29
12 Russell J,Mazzio E,Badisa RB,et al.Autoxidation of gallic acid induces ROS-dependant death in human protate cancer LNCa P cells[J].Anticancer Res,2012,32(5):1595-1602
13 Rasool MK,Sabina EP,Ramye SR,et al.Hepatoprotective and antioxidant effects of gallic acid in paracetamol-induced liver damage in mice[J].J Pharm Pharmacol,2010,62(5):638-643
14 Peng CC,Hsieh CL,Wang HE,et al.Ferulic acid is nephrodamaging while gallic acid is renal protective in long term treatment of chronic kidney disease[J].Clin Nutr,2012,31:405-414
2 李立.HPLC法测定蒙药那如三味丸中没食子酸和胡椒碱的含量[J].中国药师,2015,18(10):1830-1832
3 Rattanata N,Klaynongsruang S,Daduang S,et al.Inhibitory effects of gallic acid isolatated from caesalpinia mimosoides Lamk on cholangiocarcinoma cell lines and foodborne pathogenic bacteria[J].Asian Pac J Cancer Prev,2016,17(3):1341-1345
4 Hsu W,,Chang SP,Lin LC,et al.Limonium sinense and gallic acid suppress hepatitis C virus infection by blocking early viral entry[J].Antiviral Res,2015,118:139-147
5 张庭秀,马李杰,范贤明,等.没食子酸诱导非小细胞肺癌A549细胞凋亡的机制研究[J].中国现代医学杂志,2018,28(19):16-22
6鄢文强,李彩霞,王冬滨,等.没食子酸对人食管癌细胞生长与凋亡的影响[J].实用医学杂志,2015,31(4):540-543
7 周静,周本宏,涂杰,等.石榴皮提取物中鞣花酸和没食子酸在大鼠体内的药代动力学研究[J].中国医药导报,2015,12(3):19-23
8王琳,陈涵.药物体外吸收、分布、代谢和排泄筛选模型[J].中国组织工程研究与临床康复,2008,12(9):9957-9961
9 高林晓,郭蒙,刘凤华,等.超声浸提-高效液相色谱法测定刺三加根和茎中没食子酸含量[J].中国食品添加剂,2018,(6):145-150
10张书,林世和,郭珩,等.HPLC法测定微米大黄炭中没食子酸的含量[J].中国药师,2013,16(12):1822-1824
11 刘莉,李婷婷,孔卫东,等.HPLC法测定肠炎宁胶囊中没食子酸的含量[J].内江科技,2017(3):23,29
12 Russell J,Mazzio E,Badisa RB,et al.Autoxidation of gallic acid induces ROS-dependant death in human protate cancer LNCa P cells[J].Anticancer Res,2012,32(5):1595-1602
13 Rasool MK,Sabina EP,Ramye SR,et al.Hepatoprotective and antioxidant effects of gallic acid in paracetamol-induced liver damage in mice[J].J Pharm Pharmacol,2010,62(5):638-643
14 Peng CC,Hsieh CL,Wang HE,et al.Ferulic acid is nephrodamaging while gallic acid is renal protective in long term treatment of chronic kidney disease[J].Clin Nutr,2012,31:405-414