E2F-1与MDM2在食管鳞癌组织表达及意义
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摘要
目的探讨核转录因子-1(E2F-1)、小鼠双微体(MDM2)在食管鳞状细胞癌(食管鳞癌)组织中的表达及其意义。方法随机选取林州市肿瘤医院2008年8月—2009年3月手术切除的食管鳞癌标本49例,并同时取距肿瘤大于5cm处的正常食管组织作为对照。采用免疫组织化学方法检测E2F-1、MDM2在食管鳞癌和正常食管组织中的表达。结果食管鳞癌组织E2F-1、MDM2的表达均明显高于正常食管组织(χ~2=21.598、11.004,P<0.05),E2F-1、MDM2在食管鳞癌组织中的表达与有无淋巴结转移、分化程度、浸润深度及TNM分期有关(χ~2=4.871~11.002,P<0.05)。在食管鳞癌组织中E2F-1与MDM2的表达呈正相关(r=0.455,P<0.05)。结论E2F-1、MDM2的高表达与食管鳞癌的发生、发展有关,且二者的表达具有协同作用。
Objective To investigate the expression of E2 F-1 and MDM2 in esophageal squamous cell carcinoma(ESCC)tissue and its clinical significance. Methods A total of 49 ESCC tissue samples obtained by surgical resection in Linzhou Tumor Hospital from August 2008 to March 2009 were collected.Normal esophageal tissue samples located more than 5 cm away from the tumor were collected as controls.Immunohistochemistry was used to measure the expression of E2 F-1 and MDM2 in ESCC tissue and normal esophageal tissue. Results ESCC tissue samples had significantly higher expression of E2 F-1 and MDM2 than normal esophageal tissue samples(χ~2=21.598 and 11.004,P<0.05).The expression of E2 F-1 and MDM2 in ESCC tissue was associated with the presence or absence of lymph node metastasis,degree of tumor differentiation,depth of infiltration,and TNM stage(χ~2=4.871-11.002,P<0.05).In ESCC tissue,the expression of E2 F-1 was positively correlated with that of MDM2(r=0.455,P<0.05). Conclusion High expression of E2 F-1 and MDM2 is associated with the development and progression of ESCC,and there is a synergistic effect between them.
Objective To investigate the expression of E2 F-1 and MDM2 in esophageal squamous cell carcinoma(ESCC)tissue and its clinical significance. Methods A total of 49 ESCC tissue samples obtained by surgical resection in Linzhou Tumor Hospital from August 2008 to March 2009 were collected.Normal esophageal tissue samples located more than 5 cm away from the tumor were collected as controls.Immunohistochemistry was used to measure the expression of E2 F-1 and MDM2 in ESCC tissue and normal esophageal tissue. Results ESCC tissue samples had significantly higher expression of E2 F-1 and MDM2 than normal esophageal tissue samples(χ~2=21.598 and 11.004,P<0.05).The expression of E2 F-1 and MDM2 in ESCC tissue was associated with the presence or absence of lymph node metastasis,degree of tumor differentiation,depth of infiltration,and TNM stage(χ~2=4.871-11.002,P<0.05).In ESCC tissue,the expression of E2 F-1 was positively correlated with that of MDM2(r=0.455,P<0.05). Conclusion High expression of E2 F-1 and MDM2 is associated with the development and progression of ESCC,and there is a synergistic effect between them.
引文
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[15]吴红霞,梁建芳,郑绘霞,等.组织芯片研究胃肠道间质瘤中E2F1、MDM2及P53的表达及意义[J].山西医科大学学报,2010,41(5):418-422.
[2]BRAMIS J,ZACHARATOS P,PAPACONSTANTINOU I,et al.E2F-1transcription factor immunoexpression is inversely associated with tumor growth in colon adenocarcinomas[J].Anticancer Research,2004,24(5A):3041-3047.
[3]LEE T A,FARNHAM P J.Exogenous E2Fexpression is growth inhibitory before,during,and after cellular transformation[J].Oncogene,2000,19(18):2257-2268.
[4]刘文音,朱浩.基础研究E2F1调控细胞周期时程补偿机制的定量研究[J].南方医科大学学报,2013,33(6):870-873.
[5]HUART A S,MACLAINE N J,MEEK D W,et al.CK1alpha plays a central role in mediating MDM2control of p53and E2F-1protein stability[J].The Journal of Biological Chemistry,2009,284(47):32384-32394.
[6]STANELLE J,STIEWE T,THESELING C C,et al.Gene expression changes in response to E2F1activation[J].Nucleic Acids Research,2002,30(8):1859-1867.
[7]MEGA S,MIYAMOTO M,EBIHARA Y,et al.Cyclin D1,E2F1expression levels are associated with characteristics and prognosis of esophageal squamous cell carcinoma[J].Diseases of the Esophagus,2005,18(2):109-113.
[8]EBIHARA Y,MIYAMOTO M,SHICHINOHE T,et al.Over-expression of E2F-1in esophageal squamous cell carcinoma correlates with tumor progression[J].Diseases of the Esophagus,2004,17(2):150-154.
[9]PARK C,LEE I,KANG W K.E2F-1is a critical modulator of cellular senescence in human cancer[J].International Journal of Molecular Medicine,2006,17(5):715-720.
[10]FREEDMAN D A,WU L,LEVINE A J.Punctions of the MDM2oncoprotein[J].CMLS-Cellular and Molecular Life Sciences,1999,55(1):96-107.
[11]ZHANG R W,WANG H.MDM2oncogene as a novel target for human cancer therapy[J].Current Pharmaceutical Design,2000,6(4):393-416.
[12]WANG X Y,YANG J,HO B,et al.Interaction of helicobac-ter pylori with genetic variants in the MDM2promoter,is associated with gastric cancer susceptibility in Chinese patients[J].Helicobacter,2009,14(5):114-119.
[13]付昌征,钱军.Mdm-2、p16在大肠癌中的研究进展[J].中华全科医学,2011,9(3):441-442.
[14]CHENG T H,HSU P K,LI A F,et al.Correlation of p53,MDM2and p14(ARF)protein expression in human esophageal squamous cell carcinoma[J].Journal of Cancer Research and Clinical Oncology,2009,135(11):1577-1582.
[15]吴红霞,梁建芳,郑绘霞,等.组织芯片研究胃肠道间质瘤中E2F1、MDM2及P53的表达及意义[J].山西医科大学学报,2010,41(5):418-422.