龙眼核多酚对用脂多糖诱导的急性肺损伤小鼠肺组织的保护作用及相关机制
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摘要
目的 :分析龙眼核多酚对用脂多糖(LPS)诱导的急性肺损伤小鼠肺组织的保护作用及相关机制。方法 :将40只SPF级C57BL/6小鼠随机分为假手术组、模型组、地塞米松(DEX组)和龙眼核多酚组(LS组)。在造模前对4组小鼠连续进行3天的灌胃。在末次给药1h后,采用向小鼠的尾静脉内注射LPS的方法建立急性肺损伤小鼠模型(除假手术组小鼠以外)。在完成造模24h后,检测4组小鼠相关的指标。结果 :与模型组小鼠相比,DEX组小鼠、LS组小鼠肺泡灌洗液(BALF)中白细胞(WBC)的计数、蛋白的含量、肺湿干重比(W/D)、肝组织中丙二醛(MDA)的含量均较低,其血清中超氧化物歧化酶(SOD)的活性均较高,P <0.01。与模型组小鼠比较,LS组小鼠、DEX组小鼠肺泡的结构相对完整,其肺泡间隔的宽度和肺泡的出血量均减小,其肺泡间隔内的炎症细胞浸润较少。结论 :龙眼核多酚对用LPS诱导的急性肺损伤小鼠的肺组织具有保护的作用。龙眼核多酚的这一作用机制可能与其具有抗氧化损伤及抗炎等作用有关。
objective: to analyze the protective effect of longan polyphenols on lung tissue in mice induced by lipopolysaccharide(LPS) induced acute lung injury and its related mechanism. Methods: 40 SPF C57 BL/6 mice were randomly divided into sham operation group, model group, dexamethasone(DEX group) and longan polyphenol group(LS group). Mice in 4 groups were fed for 3 days before modeling. 1 h after the last administration, LPS was injected into the tail vein of mice to establish a mouse model of acute lung injury(except the sham group). After 24 h of modeling, the related indexes of 4 groups of mice were tested. Results: compared with the model group, WBC count, protein content, lung wet to dry weight ratio(W/D), MDA content in alveolar lavage fluid(BALF) of mice in DEX group and LS group were all lower,and superoxide dismutase(SOD) activity in serum was all higher(P < 0.01). Compared with the model group, the alveolar structure of the LS group and the DEX group was relatively complete, the width of alveolar septum and the amount of alveolar blood loss were reduced, and the infiltration of inflammatory cells in the alveolar septum was less. Conclusion: longan polyphenols can protect lung tissue in lps-induced acute lung injury mice. The mechanism of longan polyphenols may be related to its antioxidant damage and anti-inflammatory effects.
objective: to analyze the protective effect of longan polyphenols on lung tissue in mice induced by lipopolysaccharide(LPS) induced acute lung injury and its related mechanism. Methods: 40 SPF C57 BL/6 mice were randomly divided into sham operation group, model group, dexamethasone(DEX group) and longan polyphenol group(LS group). Mice in 4 groups were fed for 3 days before modeling. 1 h after the last administration, LPS was injected into the tail vein of mice to establish a mouse model of acute lung injury(except the sham group). After 24 h of modeling, the related indexes of 4 groups of mice were tested. Results: compared with the model group, WBC count, protein content, lung wet to dry weight ratio(W/D), MDA content in alveolar lavage fluid(BALF) of mice in DEX group and LS group were all lower,and superoxide dismutase(SOD) activity in serum was all higher(P < 0.01). Compared with the model group, the alveolar structure of the LS group and the DEX group was relatively complete, the width of alveolar septum and the amount of alveolar blood loss were reduced, and the infiltration of inflammatory cells in the alveolar septum was less. Conclusion: longan polyphenols can protect lung tissue in lps-induced acute lung injury mice. The mechanism of longan polyphenols may be related to its antioxidant damage and anti-inflammatory effects.
引文
[1]Han S,Mallampalli RK.The acute respiratory distress syndrome:from mechanism to translation[J].Immunol,2015,194(3):855-860.
[2]唐福才,姚敦琛,关天旺,等.龙眼核中多酚提取及抗氧化活性的研究[J].食品研究与开发,2015,36(12):5-9.
[3]唐福才,关天旺,姚敦琛,等.微波提取龙眼核中多酚及其抗氧化活性的研究[J].广东化工,2015,42(5):176-177.
[4]Kurosawa T,Miyoshi S,Yamazaki S,et al.A murine model of acute lung injury identifies growth factors to promote tissue repair and their biomarkers[J].Genes Cells.2018,12659.
[5]Ze-Wu Dong,Yu-Fang Yuan.Juglanin suppresses fibrosis and inflammation response caused by LPS in acute lung injury[J].Int J Mol Med,2018,41(6):3353-3365.
[6]Agnieszka Krupa,Marek Fol,Moshiur Rahman,et al.Silencing Bruton’s tyrosine kinase in alveolar neutrophils protects mice from LPS/immune complex-induced acute lung injury[J].Am J Physiol Lung Cell Mol Physiol,2014,307(6):L435-L448.
[2]唐福才,姚敦琛,关天旺,等.龙眼核中多酚提取及抗氧化活性的研究[J].食品研究与开发,2015,36(12):5-9.
[3]唐福才,关天旺,姚敦琛,等.微波提取龙眼核中多酚及其抗氧化活性的研究[J].广东化工,2015,42(5):176-177.
[4]Kurosawa T,Miyoshi S,Yamazaki S,et al.A murine model of acute lung injury identifies growth factors to promote tissue repair and their biomarkers[J].Genes Cells.2018,12659.
[5]Ze-Wu Dong,Yu-Fang Yuan.Juglanin suppresses fibrosis and inflammation response caused by LPS in acute lung injury[J].Int J Mol Med,2018,41(6):3353-3365.
[6]Agnieszka Krupa,Marek Fol,Moshiur Rahman,et al.Silencing Bruton’s tyrosine kinase in alveolar neutrophils protects mice from LPS/immune complex-induced acute lung injury[J].Am J Physiol Lung Cell Mol Physiol,2014,307(6):L435-L448.