电针“委中”对多裂肌损伤大鼠MG激活的影响
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摘要
目的:探讨电针"委中"对多裂肌损伤后小胶质细胞激活介导的神经炎症的治疗机制。方法:SD大鼠随机分为空白组、模型1天组、模型3天组、电针1天组、电针3天组,每组各8只。电针组双侧"委中"电针,治疗的1天、3天后同步取材。结果:模型1天组和3天组多裂肌IL-6、TNF-α、i NOS、脊髓和海马i NOS含量较空白组显著升高(P <0. 01);电针1天组多裂肌IL-6、TNF-α、i NOS含量降低(P <0. 01),脊髓和海马i NOS含量较模型1天组显著降低(P <0. 01);电针3天组多裂肌IL-6、i NOS含量降低(P> 0. 05),脊髓和海马i NOS含量较模型3天组未见统计学差异,多裂肌TNF-α含量显著低于模型3天组(P <0. 01)。结论:电针"委中"对腰多裂肌损伤模型大鼠损伤初期的神经炎症干预效果较佳,可能与其降低外周和中枢炎性因子IL-6、TNF-α、i NOS含量,抑制M1型小胶质细胞的过度激活有关。
Objective: To investigate the therapeutic mechanism of electro-needling Weizhong( BL40) on neuroinflammation mediated by microglia( MG) activation after multifidus muscle injury. Methods: SD rats were randomly divided into the blank group,the 1 d model group,the 3 d model group,the 1 d elctro-acupuncture group,and the 3 d electro-acupuncture group,with 8 rats in each group. Electro-needling BL40 was applied to the electro-acupuncture groups,the samples were collected after one day and three days of intervention.Results: The levels of IL-6,TNF-α and i NOS in the multifidus muscles,and the contents of i NOS in spinal cord and hippocampus were significantly increased in the 1 d model group compared to those in the blank group( P < 0. 01); they were greatly decreased in 1 d electro-acupuncture group when compared to those in the 1 d model group( P < 0. 01). There were no statistical difference in IL-6 and i NOS of multifidus muscle and in i NOS of hippocampus between the 3 d model group and the 3 d electro-acupuncture group( P > 0. 05);however,TNF-α content of multifidus muscle was significantly lower in the 3 d elctro-acupuncture group than that in the 3 d model group. Conclusion: Electro-needling BL40 has obvious curative effect on early neuroinflammation in rats with multifidus muscle injury. The mechanism may be related to reducing peripheral and central inflammatory factors of IL-6,TNF-α and i NOS,and inhibiting MG activation.
Objective: To investigate the therapeutic mechanism of electro-needling Weizhong( BL40) on neuroinflammation mediated by microglia( MG) activation after multifidus muscle injury. Methods: SD rats were randomly divided into the blank group,the 1 d model group,the 3 d model group,the 1 d elctro-acupuncture group,and the 3 d electro-acupuncture group,with 8 rats in each group. Electro-needling BL40 was applied to the electro-acupuncture groups,the samples were collected after one day and three days of intervention.Results: The levels of IL-6,TNF-α and i NOS in the multifidus muscles,and the contents of i NOS in spinal cord and hippocampus were significantly increased in the 1 d model group compared to those in the blank group( P < 0. 01); they were greatly decreased in 1 d electro-acupuncture group when compared to those in the 1 d model group( P < 0. 01). There were no statistical difference in IL-6 and i NOS of multifidus muscle and in i NOS of hippocampus between the 3 d model group and the 3 d electro-acupuncture group( P > 0. 05);however,TNF-α content of multifidus muscle was significantly lower in the 3 d elctro-acupuncture group than that in the 3 d model group. Conclusion: Electro-needling BL40 has obvious curative effect on early neuroinflammation in rats with multifidus muscle injury. The mechanism may be related to reducing peripheral and central inflammatory factors of IL-6,TNF-α and i NOS,and inhibiting MG activation.
引文
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[28]谢璐霜,吴巧凤,唐勇,等.电针对阿尔兹海默病大鼠海马区M2型小胶质细胞极化的影响[J].中华中医药杂志,2018,33(5):140-144.
[29]冀丽丽,葛东宇,任秀君,等.电针对自体髓核移植大鼠脊髓后角小胶质细胞活性的影响[J].北京中医药大学学报,2016,39(5):399-405.
[2]马益梅,李军,曹红,等.小胶质细胞极化与神经病理性疼痛的关系[J].国际麻醉学与复苏杂志,2015,36(10):925-928.
[3] Ma YM,Li J,Cao H,et al. Therelationship between mi-croglia polarization and neuropathic pain[J]. International Journal of Anesthesiology and Resuscitation,2015,36(10):925-928.
[4]何洁玉,吴建云,李良娟,等.神经胶质细胞对Gn RH神经元的调控机制研究进展[J].动物医学进展,2015,36(6):138-141.
[5]王伍超,郭晓丽,胡理,等.神经胶质细胞活化在神经病理性疼痛中的作用研究进展[J].中国疼痛医学杂志,2017,23(8):594-597.
[6] Wang WC,Guo XL,Hu L,et al. Research progress on roleof neuroglia activation in neuropathic pain[J]. Chinese Journal of Pain Medicine,2017,23(8):594-597.
[7] Zhao H,Alam A,Chen Q,et al. The role of microglia in the pathobiology of neuropathic pain development:what do we know?[J]. Br J Anaesth,2017,118(4):504-516.
[8] Guo JR,Wang H,Jin XJ,et al. Effect and mechanism of inhibition ofPI3K/Akt/m TOR signal pathway on chronic neuropathic pain andspinal microglia in a rat model of chronic constriction injury[J]. Oncotarget,2017,8(32):52923-52934.
[9] Echeverry S,Shi XQ,Yang M,et al. Spinal microglia are required forlong-term maintenance of neuropathic pain[J]. Pain,2017,158(9):1792-1801.
[10] Pevida M,González-Rodríguez S,Lastra A,et al. Involvement ofspinal chemokine CCL2 in the hyperalgesia evoked by bone cancer inmice:a role for astroglia and microglia[J]. Cell Mol Neurobiol,2014,34(1):143-156.
[11] Sellner S,Paricio-Montes i NOSR,Spie A,et al. Microglial CX3CR1promotes adult neurogenesis by inhibiting Sirt 1/p65 signaling inde-pendent of CX3CL1[J]. Acta Neuropathol Commun,2016,4(1):102.
[12]刘巍,陈佳,唐晓婷,等.大鼠坐骨神经损伤后早期脊髓背角小胶质细胞活化状态和活化类型的变化规律[J].中国比较医学杂志,2015,25(12):37-41,104.
[13]马龙飞,章旭,孟纯阳.神经元-小胶质细胞信号在神经病理性疼痛中的作用机制[J].中华临床医师杂志(电子版),2017(10):1807-1811.
[14] Baliki MN,Petre B,Torbey S,et al. Corticostriatal func-tional connectivity predicts transition to chronic backpain[J]. Nature Neuroscience,2012,15(8):1117-1119.
[15] Mutso AA,Radzicki D,Baliki MN,et al. Abnormalities in hippocampal functioning with persistent pain[J]. Journal of Neuroscience,2012,32(17):5747-5756.
[16] Kuwatsuka K,Hayashi H,Onoue Y,et al. The mecha-nisms of electroconvulsive stimuli in BrdU-positive cellsof the dentate gyrus in ACTH-treated rats[J]. Journal of Pharmacological Sciences,2013,122(1):34-41.
[17] Mc Ewen BS,Kalia M. The role of corticosteroids andstress in chronic pain conditions[J]. Metabolism,2010,59(1):S9-S15.
[18] Mutso AA,Petre B,Huang L,et al. Reorganization ofhippocampal functional connectivity with transition tochronic back pain[J]. Journal of Neurophysiology,2014,111(5):1065-1076.
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[20]彭园园,张莉,刘通,等.电针“委中”穴对大鼠腰多裂肌损伤后的再生促进作用及对IGF-1表达的影响[J].环球中医药,2015,210(5):513-517.
[21]邹德辉,陈玉佩,刘通,等.电针“委中”对布比卡因致大鼠腰多裂肌损伤后形态学及CK、IL-17表达的影响[J].中国针灸,2017,37(9):971-976.
[22]刘金生,李鹏,姜苏明,等.下腰部多裂肌周围神经血管分布特点及临床意义[J].局解手术学杂志,2017,26(3):167-170.
[23] Xiong XY,Liu L,Yang QW. Functions and me-cha-nisms of microglia/macrophages in neuroinflam-mation and neurogenesis after stroke[J]. Progress in Neurobiology,2016,142(7):23-44.
[24] Cherry JD,Olschowka JA,O'Banion MK. Neu-roinf-lammation and M2 microglia:the good,the bad,and the inflamed[J]. J Neuroinflamm,2014,11(1):98-112.
[25] David S,Kroner A. Repertoire of microglial and mac-rophage responses after spinal cord injury[J]. Nat Rev Neurosci,2011,12(7):388-399.
[26]赵宏,刘志顺,谢利民,等.《腰痛针灸临床实践指南》解读[J].中国针灸,2015,35(10):1065-1068.
[27]刘通,于佳妮,邹德辉,等.电针血清对多裂肌卫星细胞增殖及Pax-7、成肌分化抗原、磷酸化蛋白激酶B表达的影响[J].针刺研究,2016,41(5):402-409.
[28]谢璐霜,吴巧凤,唐勇,等.电针对阿尔兹海默病大鼠海马区M2型小胶质细胞极化的影响[J].中华中医药杂志,2018,33(5):140-144.
[29]冀丽丽,葛东宇,任秀君,等.电针对自体髓核移植大鼠脊髓后角小胶质细胞活性的影响[J].北京中医药大学学报,2016,39(5):399-405.