内生真菌对刺五加种子萌发过程激素及酶含量变化的影响
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摘要
目的研究内生真菌破除刺五加种子休眠的方法及其内源激素和酶的变化规律。方法用内生真菌侵染刺五加种子后对种子进行变温层积处理,利用高效液相色谱法测定刺五加种子中内源激素赤霉素(GA3)、脱落酸(ABA)、吲哚乙酸(IAA)、吲哚丁酸(IBA)及水杨酸(SA)的含量,并且检测体内酶[过氧化氢酶(CAT)、超氧化物歧化酶(SOD)、过氧化物酶(POD)、丙二醛(MDA)]活力变化。结果 5株刺五加内生真菌对种子萌发有明显的促进作用,在变温层积过程中,GA3、IAA、IBA、SA均有不同程度的上升,ABA呈现明显的下降趋势。POD活力和MDA水平显著下降,CAT和SOD的酶活力显著上升。结论内生真菌可调控刺五加种子激素和酶的含量,促进种子萌发。
Objective To study the methods of endophytic fungi to break the dormancy of Acanthopanax senticosus seeds and the changes of endogenous hormones and enzymes. Methods The seeds of A. senticosus were stimulated with endophytic fungi, then treated under thermophilic stratification. The content of endogenous hormones such as GA3, ABA, IAA, IBA, and SA, in the seeds of A. senticosus were tested by HPLC. And the changes of its in vivo enzymes(CAT, SOD, POD, and MDA) activity were tested. Results Five strains of endophytic fungi from A. senticosus apparently promoted the germination of seeds. In the process of fluctuating temperature stratification, the content of GA3, IAA, IBA, and SA were increased, at the same time, the content of ABA was reduced. The activity of POD and MDA enzymes was significantly reduced, and the enzyme activities of CAT and SOD increased obviously. Conclusion It is suggested that endophytes have significant effect on the content of seed hormones and enzymes, in addition endophytes could promote the initiation of seed germination.
Objective To study the methods of endophytic fungi to break the dormancy of Acanthopanax senticosus seeds and the changes of endogenous hormones and enzymes. Methods The seeds of A. senticosus were stimulated with endophytic fungi, then treated under thermophilic stratification. The content of endogenous hormones such as GA3, ABA, IAA, IBA, and SA, in the seeds of A. senticosus were tested by HPLC. And the changes of its in vivo enzymes(CAT, SOD, POD, and MDA) activity were tested. Results Five strains of endophytic fungi from A. senticosus apparently promoted the germination of seeds. In the process of fluctuating temperature stratification, the content of GA3, IAA, IBA, and SA were increased, at the same time, the content of ABA was reduced. The activity of POD and MDA enzymes was significantly reduced, and the enzyme activities of CAT and SOD increased obviously. Conclusion It is suggested that endophytes have significant effect on the content of seed hormones and enzymes, in addition endophytes could promote the initiation of seed germination.
引文
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[12]侯姣姣,余仲东,康永祥,等.内生真菌侵染对古侧柏种子萌发及幼苗生长的影响[J].西北林学院学报,2016,31(6):110-115.
[13]熊玉萍.地黄促生真菌的筛选及其作用机制的研究[D].郑州:河南中医药大学,2016.
[14]Shen L,Li L Y,Zhang X J,et al.A new indole derivative from endophyte Myrothecium roridum IFB-E091 in Artemisia annua[J].Acta Pharm Sin,2015,50:1305-1308.
[15]谭玲玲,胡正海.不同浸种处理对桔梗种子萌发和幼苗生长的影响[J].中草药,2013,44(4):468-472.
[16]Kucera B,Cohn M A,Leubner-Metzger G.Plant hormone interactions during seed dormancy release and germination[J].Seed Sci Res,2005,15(4):281-307.
[17]Rohde1 A,Bhalerao R P.Plant dormancy in the perennial context[J].Trends Plant Sci,2007,12(5):217-223.
[18]赵鑫,马小军,凯撒·苏来曼,等.低温解除阜康阿魏种子休眠和内源激素变化规律的研究[J].中草药,2006,37(2):268-270.
[19]秦国臣.内源激素ABA含量、抗氧化物酶活性和种子质量在丝瓜种子发育过程中的变化[D].杭州:浙江大学,2013.
[2]葛超,赵成昊,胡凯,等.刺五加种子休眠解除过程中过氧化物酶和酯酶同工酶的变化[J].种子,2010,29(1):50-52.
[3]汪松,解焱.中国物种红色名录(第一卷)[M].北京:高等教育出版社,2004.
[4]高成华.外源GA对刺五加种子后熟过程中内源激素含量变化的影响[J].种子,2016,35(1):35-37.
[5]Jia M,Chen L,Xin H L,et al.A friendly relationship between endophytic fungi and medicinal plants:Asystematic review[J].Front Microbiol,2016,7:906.
[6]Rey T,Schornack S.Interactions of beneficial and detrimental root-colonizing filamentous microbes with plant hosts[J].Genome Biol,2013,14:121-127.
[7]Ramalingam R,Abdul Latif K,Lee I J.Endophytic fungal pre-treatments of seeds alleviates salinity stress effects in soybean plants[J].J Microb,2013,5(6):850-857.
[8]陈疏影,尹品训,杨艳琼,等.变温层积对解除滇重楼种子休眠及其内源激素变化的研究[J].中草药,2011,42(4):793-795.
[9]齐永平,顾蔚,罗成,等.华中五味子种子的发育和3种内源激素含量的变化[J].植物生理学通讯,2009,45(6):615-618.
[10]秦国臣.内源激素ABA含量、抗氧化物酶活性和种子质量在丝瓜种子发育过程中的变化[D].杭州:浙江大学,2013.
[11]马淑兰.刺五加种子休眠机理研究[D].长春:吉林农业大学,2006.
[12]侯姣姣,余仲东,康永祥,等.内生真菌侵染对古侧柏种子萌发及幼苗生长的影响[J].西北林学院学报,2016,31(6):110-115.
[13]熊玉萍.地黄促生真菌的筛选及其作用机制的研究[D].郑州:河南中医药大学,2016.
[14]Shen L,Li L Y,Zhang X J,et al.A new indole derivative from endophyte Myrothecium roridum IFB-E091 in Artemisia annua[J].Acta Pharm Sin,2015,50:1305-1308.
[15]谭玲玲,胡正海.不同浸种处理对桔梗种子萌发和幼苗生长的影响[J].中草药,2013,44(4):468-472.
[16]Kucera B,Cohn M A,Leubner-Metzger G.Plant hormone interactions during seed dormancy release and germination[J].Seed Sci Res,2005,15(4):281-307.
[17]Rohde1 A,Bhalerao R P.Plant dormancy in the perennial context[J].Trends Plant Sci,2007,12(5):217-223.
[18]赵鑫,马小军,凯撒·苏来曼,等.低温解除阜康阿魏种子休眠和内源激素变化规律的研究[J].中草药,2006,37(2):268-270.
[19]秦国臣.内源激素ABA含量、抗氧化物酶活性和种子质量在丝瓜种子发育过程中的变化[D].杭州:浙江大学,2013.