参苓白术散对幼龄哮喘大鼠气道炎症的抑制作用
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摘要
目的探讨参苓白术散对幼龄哮喘大鼠气道炎症的影响。方法将3周龄SD雄性大鼠随机分为空白对照组、模型对照组、参苓白术散组、地塞米松组,每组10只。观察大鼠的一般情况、肺泡灌洗液细胞计数、细胞因子水平、肺组织病理变化。结果 (1)参苓白术散组、地塞米松组大鼠生理状态、哮喘症状均好转,体质量与模型对照组相比明显升高,差异有统计学意义(P<0.05);(2)参苓白术散组、地塞米松组大鼠肺泡灌洗液炎症细胞计数、白细胞介素17(IL-17)均显著低于模型对照组,IL-10、转化生长因子β1(TGF-β1)均显著高于模型对照组,差异均具有统计学意义(P<0.05);(3)参苓白术散组、地塞米松组大鼠肺组织炎症低于模型对照组。结论参苓白术散改善哮喘大鼠气道炎症可能与减轻肺组织炎性细胞浸润、降低IL-17水平和提升IL-10、TGF-β1水平有关。
Objective To investigate the effects of Shen Ling Baizhu powder on airway inflammation in young rats with bronchial asthma. Methods Three-week-old male SD rats were randomly divided into control group, model control group,Shen Ling Baizhu powder group and dexamethasone group,10 in each group. The general condition of the rats, the alveolar lavage fluid cell count, the cytokine levels, and the lung histopathology were observed.Results(1)The physiological status and asthma symptoms of the rats in the Shen Ling Baizhu powder group and the dexamethasone group were improved; the difference in body mass compared with the model control group was statistically significant(P < 0.05).(2)The inflammatory cell count and IL-17 of alveolar lavage fluid in rats of Shen Ling Baizhu powder and dexamethasone groups were significantly lower than that of the model control group;IL-10 and TGF-β1 levels were significantly higher than the model control group(P < 0.05).(3)The lung tissue inflammation of the rats in the Shen Ling Baizhu powder group and the dexamethasone group were less severe than that in the model control group. Conclusion Shen Ling Baizhu powder can improve airway inflammation in young asthmatic rats, which may be related to alleviating inflammatory cell infiltration, decreasing IL-17 level and increasing IL-10 and TGF-β1 levels.
Objective To investigate the effects of Shen Ling Baizhu powder on airway inflammation in young rats with bronchial asthma. Methods Three-week-old male SD rats were randomly divided into control group, model control group,Shen Ling Baizhu powder group and dexamethasone group,10 in each group. The general condition of the rats, the alveolar lavage fluid cell count, the cytokine levels, and the lung histopathology were observed.Results(1)The physiological status and asthma symptoms of the rats in the Shen Ling Baizhu powder group and the dexamethasone group were improved; the difference in body mass compared with the model control group was statistically significant(P < 0.05).(2)The inflammatory cell count and IL-17 of alveolar lavage fluid in rats of Shen Ling Baizhu powder and dexamethasone groups were significantly lower than that of the model control group;IL-10 and TGF-β1 levels were significantly higher than the model control group(P < 0.05).(3)The lung tissue inflammation of the rats in the Shen Ling Baizhu powder group and the dexamethasone group were less severe than that in the model control group. Conclusion Shen Ling Baizhu powder can improve airway inflammation in young asthmatic rats, which may be related to alleviating inflammatory cell infiltration, decreasing IL-17 level and increasing IL-10 and TGF-β1 levels.
引文
[1]《中华儿科杂志》编辑委员会.儿童支气管哮喘诊断与防治指南[M].中华医学会儿科学分会呼吸学组,2016.
[2]GUIBERT T W,MORGAN W J,ZEIGER R S,et al.Longterm inhaled corticosteroids in preschool children at high risk for asthma[J].N Engl J Med,2006,354(19):1985-1997.
[3]张睦涵,朱振刚.哮喘动物模型建立的研究进展.实验动物科学[J].2018,35(4):83-86.
[4]SHI Y H,SHI G C,WAN H Y,et al.Coexistence of Th1/Th2 and Th17/Treg imbalances in patient with allergic asthma[J].Chin Med J(Engl).2011,124:1951-1956.
[5]HARTL D,KOLLER B,MEHLHON A T,et al.Quantitative and functional impairment of Pulmonary CD4+CD25hi regulatory T cells in pediatric asthma[J].J Allergy Clin Immunol.2007,119(5):1258-1266.
[6]OBOKI K,OHNO T,SAITO H,et al.Thl7 and allergy[J].Allergol Int,2008,57(2):121-134.
[7]许珂玉,王艳杰,赵丹玉,等.补脾益气方药对哮喘大鼠Th1/Th2失衡的调节及机制[J].山东医药,2012,52(20):16-18.
[8]许珂玉,王艳杰,赵丹玉,等.补脾益气方药对哮喘大鼠嗜酸性粒细胞趋化因子表达的影响[J].山东医药,2012,52(24):4-7.
[9]刘健,万磊,刘磊,等.健脾通络法对类风湿关节炎调节性T细胞及肺功能的影响[J].中国临床保健杂志,2011,14(2):113-116.
[10]吴锋,孙悦,张彤,等.健脾清化方对CKD2-3期患者慢性微炎症状态的随机对照多中心研究[J].中国中西医结合肾病杂志,2012,13(6):504-506.
[11]KEARLEY J,BARKER J E,ROBINSON D S,et al.Resolution of airway inflammation and hyperreactivity after in vivo transfer of CD4+CD25+regulatory T cells is interleukin 10 dependent[J].J Exp Med,2005,202(11):1539-1547.
[12]李建民,向旭东,李金秀,等.急性哮喘患者外周血中Th17细胞的变化及其意义[J].中南大学学报医学版,2010,35(2):129-133.
[2]GUIBERT T W,MORGAN W J,ZEIGER R S,et al.Longterm inhaled corticosteroids in preschool children at high risk for asthma[J].N Engl J Med,2006,354(19):1985-1997.
[3]张睦涵,朱振刚.哮喘动物模型建立的研究进展.实验动物科学[J].2018,35(4):83-86.
[4]SHI Y H,SHI G C,WAN H Y,et al.Coexistence of Th1/Th2 and Th17/Treg imbalances in patient with allergic asthma[J].Chin Med J(Engl).2011,124:1951-1956.
[5]HARTL D,KOLLER B,MEHLHON A T,et al.Quantitative and functional impairment of Pulmonary CD4+CD25hi regulatory T cells in pediatric asthma[J].J Allergy Clin Immunol.2007,119(5):1258-1266.
[6]OBOKI K,OHNO T,SAITO H,et al.Thl7 and allergy[J].Allergol Int,2008,57(2):121-134.
[7]许珂玉,王艳杰,赵丹玉,等.补脾益气方药对哮喘大鼠Th1/Th2失衡的调节及机制[J].山东医药,2012,52(20):16-18.
[8]许珂玉,王艳杰,赵丹玉,等.补脾益气方药对哮喘大鼠嗜酸性粒细胞趋化因子表达的影响[J].山东医药,2012,52(24):4-7.
[9]刘健,万磊,刘磊,等.健脾通络法对类风湿关节炎调节性T细胞及肺功能的影响[J].中国临床保健杂志,2011,14(2):113-116.
[10]吴锋,孙悦,张彤,等.健脾清化方对CKD2-3期患者慢性微炎症状态的随机对照多中心研究[J].中国中西医结合肾病杂志,2012,13(6):504-506.
[11]KEARLEY J,BARKER J E,ROBINSON D S,et al.Resolution of airway inflammation and hyperreactivity after in vivo transfer of CD4+CD25+regulatory T cells is interleukin 10 dependent[J].J Exp Med,2005,202(11):1539-1547.
[12]李建民,向旭东,李金秀,等.急性哮喘患者外周血中Th17细胞的变化及其意义[J].中南大学学报医学版,2010,35(2):129-133.