HPLC法同时测定小儿消积化虫散中6种成分的含量
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摘要
目的:建立HPLC梯度洗脱法同时测定小儿消积化虫散中天名精内酯酮、莪术二酮、莪术醇、去氢土莫酸、猪苓酸C和茯苓酸的含量。方法:采用Agilent Eclipse XDB-C_(18)色谱柱(250 mm×4. 6 mm,5μm),以乙腈-0. 05%磷酸溶液为流动相,梯度洗脱,检测波长为210 nm,流速为0. 9 ml·min~(-1),柱温为30℃,进样体积为10μl。结果:天名精内酯酮、莪术二酮、莪术醇、去氢土莫酸、猪苓酸C和茯苓酸分别在1. 19～29. 75,2. 46～61. 50,1. 52～38. 00,3. 09～77. 25,0. 87～21. 75,3. 78～94. 50μg·ml~(-1)范围内线性关系良好,r均≥0. 999 2;平均加样回收率分别为97. 76%,98. 07%,98. 70%,99. 49%,96. 98%和100. 07%,RSD分别为1. 31%,1. 06%,1. 26%,0. 75%,1. 41%和0. 62%(n=6)。结论:本文所建立的方法操作简便、数据准确、重复性好,能够同时对小儿消积化虫散中6种成分进行含量测定,为提高和完善小儿消积化虫散的质量控制标准提供了有效的方法。
Objective: To establish an HPLC gradient elution method for the simultaneous determination of carabrone,curdione,curcumol,dehydrotumulosic acid,polyporenic acid C and pachymic acid in Xiao'er Xiaoji Huachong powder. Methods: The separation was carried out on an Agilent Eclipse XDB-C_(18) chromatographic column (250 mm × 4. 6 mm,5 μm). The mobile phase consisted of acetonitrile-0. 05% phosphoric acid solution with gradient elution. The detection wavelength was set at 210 nm. The flow rate was0. 9 ml·min~(-1),the temperature of column was set at 30 ℃,and the injection volume was 10 μl. Results: The linear range of carabrone,curdione,curcumol,dehydrotumulosic acid,polyporenic acid C and pachymic acid was 1. 19-29. 75,2. 46-61. 50,1. 52-38. 00,3. 09-77. 25,0. 87-21. 75 and 3. 78-94. 50 μg · ml~(-1) (r≥0. 999 2),respectively. The average recovery was 97. 76%,98. 07%,98. 70%,99. 49%,96. 98% and 100. 07%,and the RSD was 1. 31%,1. 06%,1. 26%,0. 75%,1. 41% and 0. 62% (n =6),respectively. Conclusion: The method with good reproducibility is simple and accurate,which can be applied in the simultaneous determination of six components in Xiao'er Xiaoji Huachong powder,and supplies another effective method for improving and perfecting the quality control of Xiao'er Xiaoji Huachong powder.
Objective: To establish an HPLC gradient elution method for the simultaneous determination of carabrone,curdione,curcumol,dehydrotumulosic acid,polyporenic acid C and pachymic acid in Xiao'er Xiaoji Huachong powder. Methods: The separation was carried out on an Agilent Eclipse XDB-C_(18) chromatographic column (250 mm × 4. 6 mm,5 μm). The mobile phase consisted of acetonitrile-0. 05% phosphoric acid solution with gradient elution. The detection wavelength was set at 210 nm. The flow rate was0. 9 ml·min~(-1),the temperature of column was set at 30 ℃,and the injection volume was 10 μl. Results: The linear range of carabrone,curdione,curcumol,dehydrotumulosic acid,polyporenic acid C and pachymic acid was 1. 19-29. 75,2. 46-61. 50,1. 52-38. 00,3. 09-77. 25,0. 87-21. 75 and 3. 78-94. 50 μg · ml~(-1) (r≥0. 999 2),respectively. The average recovery was 97. 76%,98. 07%,98. 70%,99. 49%,96. 98% and 100. 07%,and the RSD was 1. 31%,1. 06%,1. 26%,0. 75%,1. 41% and 0. 62% (n =6),respectively. Conclusion: The method with good reproducibility is simple and accurate,which can be applied in the simultaneous determination of six components in Xiao'er Xiaoji Huachong powder,and supplies another effective method for improving and perfecting the quality control of Xiao'er Xiaoji Huachong powder.
引文
1原卫生部药品标准[S].中药成方制剂.第10册.1995.12
2刘敏,周小江.HPLC同时测定天名精中5种倍半萜内酯的含量[J].中国中药杂志,2015,40(9):1783-1786
3姜国非,陆兔林,毛春芹,等.不同产地醋莪术饮片中莪术二酮,莪术醇,吉马酮和β-榄香烯含量的比较[J].中国中药杂志,2010,35(21):2834-2837
4车爽,李清,霍艳双,等.波长转换RP-HPLC法同时测定茯苓不同部位中5种三萜酸含量[J].药学学报,2010,45(4):494-497
5张靓琦,贾英,罗洁,等.超高效液相色谱法同时测定茯苓中去氢土莫酸等6种活性成分的含量[J].中国药学杂志,2012,47(13):1080-1083
6彭玮,付琴琴.基于HPLC波长切换法对舒肝益脾液中7种成分的质量控制研究[J].中国药师,2018,21(9):1667-1670
7李习平,庞雪,周逸群,等.不同加工方法对茯苓及茯苓皮中茯苓酸含量的影响[J].中国药师,2015,18(9):1453-1455
8何欢,马双成,田颂九,等.HPLC测定莪术油及其注射液中6种成分的含量[J].中国中药杂志,2010,35(5):593-597
9毛春芹,陆兔林,姜国非,等.HPLC法测定不同主产地莪术饮片中莪术二酮,莪术醇,吉马酮和β-榄香烯[J].中草药,2013,44(3):305-308
10沈玉萍,杨欢,陈斌,等.反相高效液相色谱法测定中药茯苓中4种三萜酸的含量[J].中国药学杂志,2011,46(5):388-390
2刘敏,周小江.HPLC同时测定天名精中5种倍半萜内酯的含量[J].中国中药杂志,2015,40(9):1783-1786
3姜国非,陆兔林,毛春芹,等.不同产地醋莪术饮片中莪术二酮,莪术醇,吉马酮和β-榄香烯含量的比较[J].中国中药杂志,2010,35(21):2834-2837
4车爽,李清,霍艳双,等.波长转换RP-HPLC法同时测定茯苓不同部位中5种三萜酸含量[J].药学学报,2010,45(4):494-497
5张靓琦,贾英,罗洁,等.超高效液相色谱法同时测定茯苓中去氢土莫酸等6种活性成分的含量[J].中国药学杂志,2012,47(13):1080-1083
6彭玮,付琴琴.基于HPLC波长切换法对舒肝益脾液中7种成分的质量控制研究[J].中国药师,2018,21(9):1667-1670
7李习平,庞雪,周逸群,等.不同加工方法对茯苓及茯苓皮中茯苓酸含量的影响[J].中国药师,2015,18(9):1453-1455
8何欢,马双成,田颂九,等.HPLC测定莪术油及其注射液中6种成分的含量[J].中国中药杂志,2010,35(5):593-597
9毛春芹,陆兔林,姜国非,等.HPLC法测定不同主产地莪术饮片中莪术二酮,莪术醇,吉马酮和β-榄香烯[J].中草药,2013,44(3):305-308
10沈玉萍,杨欢,陈斌,等.反相高效液相色谱法测定中药茯苓中4种三萜酸的含量[J].中国药学杂志,2011,46(5):388-390