大鼠口服艳山姜挥发油后的入血成分分析
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摘要
目的:检测艳山姜挥发油(EOFAZ)进入大鼠血浆的成分。方法:采用GC-MS技术和血清药物化学研究方法,分析大鼠经EOFAZ灌胃1 h后的入血成分。结果:建立了大鼠给药后血浆样品的分析测定方法,并与空白血浆样品及EOFAZ对比,检测并鉴定出37个入血成分,且均为挥发油原型成分,主要活性成分β-蒎烯、α-蒎烯、莰烯、1,8-桉叶油醇在EOFAZ及大鼠血浆中所占百分含量均较高。结论:艳山姜活性部位37种挥发油进入大鼠血浆,为艳山姜作用的药效基础。
Objective: To investigate the prototype components of the essential oil from Fructus Alpiniae Zerumbet(EOFAZ) in rats plasma. Methods: GC-MS and serum pharmacochemistry were used to analyze the prototype components of EOFAZ one hour after intragastric infusion in rats. Results: The developed method was applicable to the analysis and identification of metabolites of EOFAZ after oral administration. The 37 prototype components were identified in comparison with that of the blank biological samples and EOFAZ. The main active ingredients of β-pinene,α-pinene,terpene,and 1,8-Cineole were all higher in EOFAZ and blood components. Conclusion: The components of essential oil from active fractions of Alpiniae Zerumbet in rats serum provides a certain reference for the further study of the vivo process and pharmacodynamics material basis of EOFAZ.
Objective: To investigate the prototype components of the essential oil from Fructus Alpiniae Zerumbet(EOFAZ) in rats plasma. Methods: GC-MS and serum pharmacochemistry were used to analyze the prototype components of EOFAZ one hour after intragastric infusion in rats. Results: The developed method was applicable to the analysis and identification of metabolites of EOFAZ after oral administration. The 37 prototype components were identified in comparison with that of the blank biological samples and EOFAZ. The main active ingredients of β-pinene,α-pinene,terpene,and 1,8-Cineole were all higher in EOFAZ and blood components. Conclusion: The components of essential oil from active fractions of Alpiniae Zerumbet in rats serum provides a certain reference for the further study of the vivo process and pharmacodynamics material basis of EOFAZ.
引文
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[12]邵君傲,李佳川,曾敏,等.民族药艳山姜提取物对实验性动脉粥样硬化大鼠血管舒缩因子的影响[J].西南民族大学学报(自然科学版),2015,41(5):571-574.
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[14]文波,令狐克刚,徐旖旎,等.艳山姜挥发油抑制JNK1/2/3磷酸化对ox-LDL诱导的HAECs 损伤的影响[J].中国实验方剂学杂志,2015,21(22):112-115.
[15]付凌云,杨红,林丹,等.EOFAZ抑制TNF-α诱导的血管新生和炎症损伤[J].中国药理学通报,2018,34(11):1594-1599.
[16]吴朝花,严俊丽,王益,等.艳山姜挥发油自乳化释药系统的优化及评价[J].中草药,2017,48(19):3970-3976.
[17]张科,严俊丽,李婉蓉,等.氧化苦参碱磷脂复合物纳米结构脂质载体的制备[J].贵州医科大学学报,2018,43(11):1256-1262.
[18]毕肖林,马世堂,狄留庆,等.中药药效物质筛选与辨识的研究思路及进展[J].中草药,2018,49(22):5229-5234.
[19]李志红,颜仁杰,邢建国,等.基于血清药物化学研究香青兰抗心肌缺血活性部位的活性成分[J].中国医药工业杂志,2018,49(8):1142-1148.
[20]武晓伟,郝艳艳,聂春霞,等.中药血清药物化学的方法学概述及应用领域研究进展[J].中国实验方剂学杂志,2018,24(20):15-21.
[21]朱凯,张燚曼,冯青,等.人参入血成分对过氧化氢损伤的心肌细胞的保护作用[J].中国老年学杂志,2018,38(10):2457-2459.
[22]张王宁,李爱平,李科,等.中药药效物质基础研究方法进展[J].中国药学杂志,2018,53(10):761-764.
[23]汪小莉,刘晓,韩燕全,等.中药药效物质基础主要研究方法概述[J].中草药,2018,49(4):941-947.
[2] 吴林菁,肖瑞瑶,李晨,等.艳山姜果实的生药学及气相色谱研究[J].广西植物,2017,37(8):1043-1048.
[3] 吴林菁,姜丰,苏菊,等.艳山姜挥发油提取工艺优选及化学成分GC-MS分析[J].贵州医科大学学报,2017,42(6):655-660.
[4] 张旭,何明辉,郑伟耀,等.艳山姜果实挥发油的提取工艺优化及其化学成分分析[J].中国民族民间医药,2017,26(8):14-17.
[5] 田璧榕,沈祥春,潘迪,等.艳山姜叶的化学成分研究[J].中药材,2018,41(11):2316-2319.
[6] 张彦燕,赵爽 ,付凌云,等.艳山姜挥发油对TGF-β-1诱导内皮细胞氧化应激损伤的保护作用研究[J].西南民族大学学报(自然科学版),2018,44(6):556-560.
[7] 张彦燕,令狐克刚,陈妍,等.正交试验法研究艳山姜挥发油保护脂多糖诱导HUVEC损伤的活性组分[J].中国实验方剂学志,2014,20(20):9-12.
[8] 林丹,石廷雨,杨红,等.艳山姜挥发油调控NF-κB信号抑制LPS诱导HAECs炎性损伤[J].中国实验方剂学杂志,2018,24(19):139-144.
[9] 杨红,林丹,李晨,等.艳山姜挥发油对高糖诱导大鼠视网膜Müller细胞VEGF表达的调控作用[J].中药材,2018,41(3):686-690.
[10]李晨,赵爽,徐旖旎,等.艳山姜挥发油对TGF-β1诱导人脐静脉内皮细胞间质转分化的保护作用[J].中国实验方剂学杂志,2017,23(22):160-164.
[11]HUANG N,XU Y,ZHOU H,et al.Essential oil from fructus Alpiniae zerumbet protects human umbilical vein endothelial cells in vitro from injury induced by high glucose levels by suppressing nuclear transcription factor-Kappa B signaling.[J].Med Sci Monit,2017,4(23):4760-4767.
[12]邵君傲,李佳川,曾敏,等.民族药艳山姜提取物对实验性动脉粥样硬化大鼠血管舒缩因子的影响[J].西南民族大学学报(自然科学版),2015,41(5):571-574.
[13]XIAO R Y,WU L J,HONG X X,et al.Screening of analgesic and anti-inflammatory active component in Fructus Alpiniae zerumbet based on spectrum-effect relationship and GC-MS[J].Biomedical Chromatography,2018,32(3):e4112.
[14]文波,令狐克刚,徐旖旎,等.艳山姜挥发油抑制JNK1/2/3磷酸化对ox-LDL诱导的HAECs 损伤的影响[J].中国实验方剂学杂志,2015,21(22):112-115.
[15]付凌云,杨红,林丹,等.EOFAZ抑制TNF-α诱导的血管新生和炎症损伤[J].中国药理学通报,2018,34(11):1594-1599.
[16]吴朝花,严俊丽,王益,等.艳山姜挥发油自乳化释药系统的优化及评价[J].中草药,2017,48(19):3970-3976.
[17]张科,严俊丽,李婉蓉,等.氧化苦参碱磷脂复合物纳米结构脂质载体的制备[J].贵州医科大学学报,2018,43(11):1256-1262.
[18]毕肖林,马世堂,狄留庆,等.中药药效物质筛选与辨识的研究思路及进展[J].中草药,2018,49(22):5229-5234.
[19]李志红,颜仁杰,邢建国,等.基于血清药物化学研究香青兰抗心肌缺血活性部位的活性成分[J].中国医药工业杂志,2018,49(8):1142-1148.
[20]武晓伟,郝艳艳,聂春霞,等.中药血清药物化学的方法学概述及应用领域研究进展[J].中国实验方剂学杂志,2018,24(20):15-21.
[21]朱凯,张燚曼,冯青,等.人参入血成分对过氧化氢损伤的心肌细胞的保护作用[J].中国老年学杂志,2018,38(10):2457-2459.
[22]张王宁,李爱平,李科,等.中药药效物质基础研究方法进展[J].中国药学杂志,2018,53(10):761-764.
[23]汪小莉,刘晓,韩燕全,等.中药药效物质基础主要研究方法概述[J].中草药,2018,49(4):941-947.